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Reliability Bounds based on Universal Generating Function and Discrete Stress-strength Interference Model
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作者 GUO Hui-xin SUO Bin ZHANG Gan-qing 《International Journal of Plant Engineering and Management》 2017年第3期175-187,共13页
A method for estimating the component reliability is proposed when the probability density functions of stress and strength can not be exactly determined. For two groups of finite experimental data about the stress an... A method for estimating the component reliability is proposed when the probability density functions of stress and strength can not be exactly determined. For two groups of finite experimental data about the stress and strength, an interval statistics method is introduced. The processed results are formulated as two interval-valued random variables and are graphically represented component reliability are proposed based on the by using two histograms. The lower and upper bounds of universal generating function method and are calculated by solving two discrete stress-strength interference models. The graphical calculations of the proposed reliability bounds are presented through a numerical example and the confidence of the proposed reliability bounds is discussed to demonstrate the validity of the proposed method. It is showed that the proposed reliability bounds can undoubtedly bracket the real reliability value. The proposed method extends the exciting universal generating function method and can give an interval estimation of component reliability in the case of lake of sufficient experimental data. An application example is given to illustrate the proposed method 展开更多
关键词 universal generating function discrete stress-strength interference model reliability bounds reliability estimation
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Improved Discrete Interval-Valued Stress-Strength Interference Model Based on Extended Universal Generating Function
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作者 高建雄 安宗文 白学宗 《Journal of Donghua University(English Edition)》 EI CAS 2016年第2期305-307,共3页
In practical engineering,sometimes the probability density functions( PDFs) of stress and strength can not be exactly determined,or only limited experiment data are available. In these cases,the traditional stress-str... In practical engineering,sometimes the probability density functions( PDFs) of stress and strength can not be exactly determined,or only limited experiment data are available. In these cases,the traditional stress-strength interference( SSI) model based on classical probabilistic approach can not be used to evaluate reliabilities of components. To solve this issue, the traditional universal generating function( UGF) is introduced and then it is extended to represent the discrete interval-valued random variable.Based on the extended UGF,an improved discrete interval-valued SSI model is proposed, which has higher calculation precision compared with the existing methods. Finally,an illustrative case is given to demonstrate the validity of the proposed model. 展开更多
关键词 RELIABILITY universal generating function(UGF) discrete interval-valued stress-strength interference(SSI) model
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Refinement of Adaptive Dynamical Simulation of Quantum Mechanical Double Slit Interference Phenomenon
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作者 Tadashi Ando Andrei Khrennikov Ichiro Yamato 《Journal of Modern Physics》 2024年第3期239-249,共11页
We applied adaptive dynamics to double slit interference phenomenon using particle model and obtained partial successful results in our previous report. The patterns qualitatively corresponded well with experiments. S... We applied adaptive dynamics to double slit interference phenomenon using particle model and obtained partial successful results in our previous report. The patterns qualitatively corresponded well with experiments. Several properties such as concave single slit pattern and large influence of slight displacement of the emission position were different from the experimental results. In this study we tried other slit conditions and obtained consistent patterns with experiments. We do not claim that the adaptive dynamics is the principle of quantum mechanics, but the present results support the probability of adaptive dynamics as the candidate of the basis of quantum mechanics. We discuss the advantages of the adaptive dynamical view for foundations of quantum mechanics. 展开更多
关键词 Double Slit interference Adaptive Dynamics Quantum Mechanics Particle model Simulation
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Reliability Analysis of Systems with Common Cause Failure Based on Stress-Strength Interference Model 被引量:3
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作者 ZHANG Shuai LU Ruoning +1 位作者 SI Shubin REN Fangyu 《Journal of Shanghai Jiaotong university(Science)》 EI 2018年第5期707-710,共4页
Many mechanical systems have the characteristics of multiple failure modes and complex failure mech- anisms. On the basis of stress-strength interference (SSI) model, this paper takes the mechanical system with comm... Many mechanical systems have the characteristics of multiple failure modes and complex failure mech- anisms. On the basis of stress-strength interference (SSI) model, this paper takes the mechanical system with common cause failure (CCF) as the research object. The relationship between the stress distribution and the strength distribution is studied, and the failures of components are independent of each other under the determin- istic stress. Then, the concept of conditional reliability is introduced to build the system reliability models under the action of one-stress and multi-stress for both series and parallel systems. Finally, the corresponding properties of the DrODosed methods are discussed to show their advantages. 展开更多
关键词 system reliability stress-strength interference (SSI) model common cause failure (CCF)
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Reliability Assessment Method Based on Interference Variable for Stress-Strength Model
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作者 吴琼 杨建中 +2 位作者 王晶燕 满剑锋 孙国鹏 《Journal of Donghua University(English Edition)》 EI CAS 2015年第6期1047-1051,共5页
Stress-strength model is a basic and important tool for reliability analysis.There are few methods to assess the confidence limit of interference reliability when the distribution parameters of stress and strength are... Stress-strength model is a basic and important tool for reliability analysis.There are few methods to assess the confidence limit of interference reliability when the distribution parameters of stress and strength are all unknown.A new assessment method of interference reliability is proposed and the estimates of the distribution parameters are accordingly given.The lower confidence limit of interference reliability with given confidence can be obtained with the method even though the parameters are all unknown.Simulation studies and an engineering application are conducted to validate the method,which suggest that the method provides precise estimates even for sample size of approximately. 展开更多
关键词 interference reliability stress-strength mode(SSM) lower confidence limit of reliability small sample size highly reliable product
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Well interference evaluation considering complex fracture networks through pressure and rate transient analysis in unconventional reservoirs 被引量:2
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作者 Jia-Zheng Qin Qian-Hu Zhong +2 位作者 Yong Tang Wei Yu Kamy Sepehrnoori 《Petroleum Science》 SCIE EI CAS CSCD 2023年第1期337-349,共13页
Severe well interference through complex fracture networks(CFNs)can be observed among multi-well pads in low permeability reservoirs.The well interference analysis between multi-fractured horizontal wells(MFHWs)is vit... Severe well interference through complex fracture networks(CFNs)can be observed among multi-well pads in low permeability reservoirs.The well interference analysis between multi-fractured horizontal wells(MFHWs)is vitally important for reservoir effective development.Well interference has been historically investigated by pressure transient analysis,while it has shown that rate transient analysis has great potential in well interference diagnosis.However,the impact of complex fracture networks(CFNs)on rate transient behavior of parent well and child well in unconventional reservoirs is still not clear.To further investigate,this paper develops an integrated approach combining pressure and rate transient analysis for well interference diagnosis considering CFNs.To perform multi-well simulation considering CFNs,non-intrusive embedded discrete fracture model approach was applied for coupling fracture with reservoir models.The impact of CFN including natural fractures and frac-hits on pressure and rate transient behavior in multi-well system was investigated.On a logelog plot,interference flow and compound linear flow are two new flow regimes caused by nearby producers.When both NFs and frac-hits are present in the reservoir,frac-hits have a greater impact on well#1 which contains frac-hits,and NFs have greater impact on well#3 which does not have frac-hits.For all well producing circumstances,it might be challenging to see divergence during pseudosteady state flow brought on by frac-hits on the logelog plot.Besides,when NFs occur,reservoir depletion becomes noticeable in comparison to frac-hits in pressure distribution.Application of this integrated approach demonstrates that it works well to characterize the well interference among different multi-fractured horizontal wells in a well pad.Better reservoir evaluation can be acquired based on the new features observed in the novel model,demonstrating the practicability of the proposed approach.The findings of this study can help for better evaluating well interference degree in multi-well systems combing PTA and RTA,which can reduce the uncertainty and improve the accuracy of the well interference analysis based on both field pressure and rate data. 展开更多
关键词 Well interference Numerical rate transient analysis Numerical pressure transient analysis Complex fracture networks Embedded discrete fracture model
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Digital Cancellation Scheme and Hardware Implementation for High-Order Passive Intermodulation Interference Based on Hammerstein Model 被引量:5
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作者 Xiaqing Miao Lu Tian 《China Communications》 SCIE CSCD 2019年第9期165-176,共12页
Passive intermodulation(PIM)interference urgently needs to be solved in the satellite communication system,owing to degrading the whole performance.Mainstream research contributions to the cancellation method for PIM ... Passive intermodulation(PIM)interference urgently needs to be solved in the satellite communication system,owing to degrading the whole performance.Mainstream research contributions to the cancellation method for PIM were focused on the analog domain,however,the PIM distortion cannot be eliminated completely with the approaches.Meanwhile,some researchers attempt to tackle the problem through digital signal processing,nevertheless,the proposed methods were not suitable for the practical satellite communication scenario.In this paper,we present a general scheme for the adaptive feedforward PIM cancellation.High-order PIM signals at baseband are estimated by modeling the PIM distortion with Hammerstein model in the digital domain.Based on the reconstructed PIM signal,we adopt the least mean square algorithm to adaptively mitigate the PIM interference for tracking the variation of PIM.The time and frequency synchronization of PIM are based on the correlation of the peak of received signals with the corresponding reconstructed PIM signal.Practical experimental results show that the scheme can effectively cancel the PIM interference,and achieve an interference suppression gain more than 20dB. 展开更多
关键词 satellite communication passiveintermodulation interference DIGITAL CANCELLATION HAMMERSTEIN model
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Physics-based analysis and simulation model of electromagnetic interference induced soft logic upset in CMOS inverter 被引量:3
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作者 Yu-Qian Liu Chang-Chun Chai +4 位作者 Yu-Hang Zhang Chun-Lei Shi Yang Liu Qing-Yang Fan Yin-Tang Yang 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第6期531-538,共8页
The instantaneous reversible soft logic upset induced by the electromagnetic interference(EMI) severely affects the performances and reliabilities of complementary metal–oxide–semiconductor(CMOS) inverters. This... The instantaneous reversible soft logic upset induced by the electromagnetic interference(EMI) severely affects the performances and reliabilities of complementary metal–oxide–semiconductor(CMOS) inverters. This kind of soft logic upset is investigated in theory and simulation. Physics-based analysis is performed, and the result shows that the upset is caused by the non-equilibrium carrier accumulation in channels, which can ultimately lead to an abnormal turn-on of specific metal–oxide–semiconductor field-effect transistor(MOSFET) in CMOS inverter. Then a soft logic upset simulation model is introduced. Using this model, analysis of upset characteristic reveals an increasing susceptibility under higher injection powers, which accords well with experimental results, and the influences of EMI frequency and device size are studied respectively using the same model. The research indicates that in a range from L waveband to C waveband, lower interference frequency and smaller device size are more likely to be affected by the soft logic upset. 展开更多
关键词 electromagnetic interference soft logic upset non-equilibrium carrier upset model
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Interference Resistance of Pentamaran Ship Model With Asymmetric Outrigger Configurations 被引量:2
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作者 Yanuar Ibadurrahman +2 位作者 Kurniawan T. Waskito S. Karim M. Ichsan 《Journal of Marine Science and Application》 CSCD 2017年第1期42-47,共6页
An experimental investigation is performed to assess the relation of interference performance on the total resistance of a pentamaran model advancing in calm water. For this motivation, the total drag of the ship is ... An experimental investigation is performed to assess the relation of interference performance on the total resistance of a pentamaran model advancing in calm water. For this motivation, the total drag of the ship is performed for several values of asymmetric outrigger configuration and hull separation, altering the Froude number in the range 0.3–0.9. Our results indicate that remarkable changes in resistance require notable changes in transverse distance values (hull separation) when wave interference may occur. In addition, there is no single configuration that consistently outperforms the other configurations across the entire speed range and the optimum interference factor -0.2 appears at a Froude number of 0.45 in S/L=0.33 with the outrigger outer position: asymmetric outboard for A3 configuration. 展开更多
关键词 interference resistance interference factor asymmetric outrigger multihull ship PENTAMARAN ship model
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Stability Analysis and Hopf Bifurcation for ODE System of Predator-Prey Model with Mutual Interference 被引量:3
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作者 Khalid Ahmed Abbakar Yafei Yang +3 位作者 Alhussein Mohamed Songchen Xia Mogahid Mamoon Abkar Omer Bushra Elfadil Hassan 《Applied Mathematics》 2021年第9期793-802,共10页
In this paper, the temporal and spatial patterns of a diffusive predator-prey model with mutual interference under homogeneous Neumann boundary conditions were studied. Specifically, first, taking the intrinsic growth... In this paper, the temporal and spatial patterns of a diffusive predator-prey model with mutual interference under homogeneous Neumann boundary conditions were studied. Specifically, first, taking the intrinsic growth rate of the predator as the parameter, we give a computational and theoretical analysis of Hopf bifurcation on the positive equilibrium for the ODE system. As well, we have discussed the conditions for determining the bifurcation direction and the stability of the bifurcating periodic solutions. 展开更多
关键词 Predator-Prey model Mutual interference Hopf Bifurcation Functional Response
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Horizontal Well Interference Performance and Water Injection Huff and Puff Effect on Well Groups with Complex Fracture Networks:A Numerical Study
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作者 Haoyu Fu Hua Liu +1 位作者 Xiaohu Hu Lei Wang 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第12期2285-2309,共25页
Well interference has become a common phenomenon with the increasing scale of horizontal well fracturing.Recent studies on well interference in horizontal wells do not properly reflect the physical model of the postfr... Well interference has become a common phenomenon with the increasing scale of horizontal well fracturing.Recent studies on well interference in horizontal wells do not properly reflect the physical model of the postfracturing well groups and the realistic fracturing process of infill wells.Establishing the correspondence between well interference causative factors and manifestations is of great significance for infill well deployment and secondary oil recovery.In this work,we develop a numerical model that considers low velocity non-Darcy seepage inshale reservoirs to study the inter-well interferencephenomenon that occurs in theSantanghufield,andconstruct an explicit hydraulic fracture and complex natural fracture network model with an embedded discrete fracture model,focusing on the effect of fracture network morphology on well interactions.The model also considers a multi-segment wellbore model to accommodate the effect of inter-well crossflow on wellbore tubular flow.The changes in formation pressure and water saturation during fracturing are performed by controlling the injection pressure and water injection rate.The result shows that the shape of the fracture network generated by the infill well with the old well determines the subsequent fluid and oil-increasing performance of the disturbed well.The synergistic production or competitive relationship formed by fractures with different connectivity between the two wells determines the positive and negative effects of the interference.The paper also investigates the adaptation study of water injection huff and puff schemes for well groups with different connectivity,and demonstrated a potential yield increase of up to 10.85%under adaptation injection.This method of identifying well interference based on the production dynamics of affected wells and the subsequent corresponding water injection method provides valuable references for the selection of secondary oil recovery measures. 展开更多
关键词 Unconventional reservoirs well interference embedded discrete fracture model water injection huff and puff complex fracture networks
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Interferences in Photodetachment of a Negative Molecular Ion Model 被引量:1
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作者 A. Afaq DU Meng-Li 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第12期1401-1406,共6页
By employing a two-center model, the total and differential cross sections in the photodetachment of "a negative molecular ion" are studied theoreticedly and obtained for the case of light polarization paredlel to t... By employing a two-center model, the total and differential cross sections in the photodetachment of "a negative molecular ion" are studied theoreticedly and obtained for the case of light polarization paredlel to the molecular axis. We find that in contrast to the smooth behavior of the total cross section for perpendicular polarized light, the cross section for parallel polarized light shows an interesting oscillatory structure. The oscillations in the toted cross section may provide a method to determine the distance between the two centers. We explain the oscillation in the toted cross section as an interference effect using closed-orbit theory. We also cedculated the detached-electron flux distributions on a screen placed at a large distance from the negative molecular ion. The distributions display multiple-ring-like interference patterns. Such interference patterns are similar to those in the photodetachment microscopy experiments. 展开更多
关键词 quantum interference two-center model PHOTODETACHMENT
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Calculating Model of Interference Amount for Miniaturized Gear and Shaft Shrink Fit
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作者 金鑫 张之敬 +1 位作者 叶鑫 李忠新 《Journal of Beijing Institute of Technology》 EI CAS 2006年第2期138-143,共6页
Based on miniaturized components' characteristics, the method of assembling miniaturized gear and shaft together with corresponding calculating model of the interference amount are proposed. On the basis of main effe... Based on miniaturized components' characteristics, the method of assembling miniaturized gear and shaft together with corresponding calculating model of the interference amount are proposed. On the basis of main effecting factors analysis on the gear and shaft assembling interference amount, calculating formula including all factors effective on the interference amount necessary for reliable system running was built up. The methods of reverse calculating theoretical model was used to build up the equivalent simulation model of the theoretical one, together with simulation verification and case study for calculating formula. The results show that the cold assembling method is applicable for miniaturized gear and shaft, but in the assembling process, the interference amount compensating the shape error of contacting surfaces takes a large proportion, which is the main cause of stress variance on contacting surfaces. 展开更多
关键词 MINIATURIZED interference amount calculating model cold assembling simulation model
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Reliability analysis of diesel engine crankshaft based on 2D stress strength interference model
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作者 周迅 俞小莉 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2006年第3期391-397,共7页
A 2D stress strength interference model (2D-SSIM) considering that the fatigue reliability of engineering structural components has close relationship to load asymmetric ratio and its variability to some extent is put... A 2D stress strength interference model (2D-SSIM) considering that the fatigue reliability of engineering structural components has close relationship to load asymmetric ratio and its variability to some extent is put forward. The principle, geo-metric schematic and limit state equation of this model are presented. Reliability evaluation for a kind of diesel engine crankshaft was made based on this theory, in which multi-axial loading fatigue criteria was employed. Because more important factors, i.e. stress asymmetric ratio and its variability, are considered, it theoretically can make more accurate evaluation for structural com-ponent reliability than the traditional interference model. Correspondingly, a Monte-Carlo Method simulation solution is also given. The computation suggests that this model can yield satisfactory reliability evaluation. 展开更多
关键词 Fatigue reliability 2-dimensional interference model (2D-SSIM) Monte-Carlo Method Load asymmetric ratio Multi-axial fatigue criteria
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Distributed link scheduling method with physical interference model in wireless multi-hop networks
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作者 樊帅 Zhang Lin +1 位作者 Feng Wei Ren Yong 《High Technology Letters》 EI CAS 2013年第4期353-358,共6页
To further increase the throughput of wireless multi-hop networks,a distributed scheduling method is proposed,which takes physical interference model into account.It is assumed that nodes in the network can perform ph... To further increase the throughput of wireless multi-hop networks,a distributed scheduling method is proposed,which takes physical interference model into account.It is assumed that nodes in the network can perform physical carrier sensing,and the carrier sensing range can be set to different values.In the traditional carrier sensing mechanism,the carrier sensing range is computed under the protocol interference model,which is not accurate.Here the optimal carrier sensing range with physical interference model is achieved.Each sending node implements the distributed approach in three phases at each time slot,and all the concurrent transmissions are interference free.Good performance can be achieved under this scheduling approach.The approximation ratio of the distributed method to the optimal one is also proved. 展开更多
关键词 wireless multi-hop network physical interference model distributed scheduling physical carrier sensing
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Study on intercarrier interference in mobile MIMO-OFDM based on the geometrical one-ring model
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作者 Li Juhu Zhi Xiyun +1 位作者 He Zhiqiang Wu Weiling 《High Technology Letters》 EI CAS 2012年第1期20-25,共6页
This paper investigates the distribution of intercarrier interference (ICI) in multiple-input multiple-output orthogonal frequency division multiplexing (MIMO-OFDM) systems based on the geometrical one-ring model.... This paper investigates the distribution of intercarrier interference (ICI) in multiple-input multiple-output orthogonal frequency division multiplexing (MIMO-OFDM) systems based on the geometrical one-ring model. Using the spatial and temporal correlation of a geometrical one-ring model, a close-formed expression of intercarrier interference due to the Doppler effect caused by the movement of receiver is derived under the isotropic scattering conditions and non-isotropic scattering conditions. The analytical results are verified by Monte Carlo simulations. We use the generated channels to investigate MIMO-OFDM intercarrier interference under various channel parameters. It can be shown that more than 95% oflCI power comes from five neighboring subcarriers. 展开更多
关键词 intercarrier interference (ICI) one-ring model spatial and temporal correlation isotropicscattering non-isotropic scattering
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Interference Cancellation with an Achievable Angular Channel Model Based on Coordinate Multi-Point
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作者 Md. Abdul Latif Sarker Moon Ho Lee 《通讯和计算机(中英文版)》 2013年第3期301-308,共8页
关键词 信道模型 干扰消除 点坐标 最小均方误差 LTE 蜂窝系统 干扰抵消 协调系统
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Radio Interference Characteristic of Ultra-high Voltage AC Transmission Lines by Using Stochastic Modeling
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作者 HE Wangling WAN Baoquan +3 位作者 PEI Chunming ZHANG Jiangong HE Junjia GUO Haozhou 《高电压技术》 EI CAS CSCD 北大核心 2013年第10期2438-2444,共7页
For developing ultra-high voltage(UHV) AC power transmission systems,it is important to precisely estimate and to limit the radio interference(RI) level of power lines.Based on the stochastic characteristics in amplit... For developing ultra-high voltage(UHV) AC power transmission systems,it is important to precisely estimate and to limit the radio interference(RI) level of power lines.Based on the stochastic characteristics in amplitude and repetition rate of induced corona current,by using the probability theory and mathematical statistics,we establish a stochastic model for the wide-sense stationary random process of corona discharges.Then combining the stochastic model with model-propagation-analysis method,the RI levels under three-phase UHV AC transmission lines are calculated.The results of the calculation based on stochastic model method and International Council on Large Electric Systems(CIGRE) excitation function are compared with that based on semi-empirical method and some other excitation functions.The stochastic model based on different excitation functions is also adopted to simulate the RI levels under finite test lines with two opened terminations.The results indicate that with the same average maximum gradient on conductor surface and the same conductor type,the number of corona discharge per unit length is one of the main reasons that causes the difference between different excitation functions.It is also concluded that for a long test line,the effect of standing wave on RI field strength is negligible in the middle of the line,but obvious near both terminations: for a 10-km line,the maximum difference in RI field strength is 2.78 dB,between the peak value of the standing wave near the ends and the steady value near the middle of the line. 展开更多
关键词 特高压输电线路 交流输电线路 无线电干扰 随机建模 干扰特性 概率论与数理统计 电力传输系统 平稳随机过程
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Stress-Strength Structural Reliability Model with a Stochastic Strength Aging Deterioration Process 被引量:5
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作者 叶欢 陈庆华 《Journal of Donghua University(English Edition)》 EI CAS 2014年第6期847-849,共3页
A stress-strength structural reliability model was proposed with a stochastic strength aging deterioration process. In structural engineering,the deterioration of structure's strength should be the total of the de... A stress-strength structural reliability model was proposed with a stochastic strength aging deterioration process. In structural engineering,the deterioration of structure's strength should be the total of the deterioration owing to continual wear, fatigue,corrosion,etc.,and the abrupt deterioration as a result of randomly variable loads. The deterioration of structure's strength should be influenced by both the internal deterioration owing to direct wear and the external deterioration due to randomly variable loads.Meanwhile,the load process was given as Poisson square wave process. The reliability was derived using stress-strength interference theory. In particular,the reliability was also given when random variables followed the normal distribution. 展开更多
关键词 stress-strength interference internal DETERIORATION external DETERIORATION randomly variable loads POISSON SQUARE wave process
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Silencing of signal transducer and activator of transcription 3 expression by RNA interference suppresses growth of human hepatocellular carcinoma in tumor-bearing nude mice 被引量:13
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作者 Jing Li Yun-Feng Piao +2 位作者 Zheng Jiang Li Chen Hai-Bo Sun 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第21期2602-2608,共7页
AIM: To explore the effect of silencing of signal transducer and activator of transcription 3 (STAT3) expression by RNA interference (RNAi) on growth of human hepatocellular carcinoma (HCC) in tumorbearing nude... AIM: To explore the effect of silencing of signal transducer and activator of transcription 3 (STAT3) expression by RNA interference (RNAi) on growth of human hepatocellular carcinoma (HCC) in tumorbearing nude mice in vivo.METHODS: To construct the recombinant plasmid of pSilencer 3.0-H1-STAT3-siRNA-GFP (pSHI-siRNA- STAT3) and establish the tumor-bearing nude mouse model of the HCC cell line SMMC7721, we used intratumoral injection together with electroblotting to transfect the recombinant plasmid pSHI-siRNA- STAT3 into the transplanted tumor. The weight of the nude mice and tumor volumes were recorded. STAT3 gene transcription was detected by semi-quantitative reverse transcription polymerase chain reaction (RT- PCR). Level of protein expression and location of STAT3 were determined by Western blotting and immunohistochemical staining. STAT3-related genes such as survivin, c-myc, VEGF, p53 and caspase3 mRNA and protein expression were detected in tumor tissues at the same time. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was used to detect apoptosis of tumor cells.RESULTS: The weight of the treated nude mice increased, and the tumor volume decreased markedly compared with those of the mock-treated and negative control groups (P 〈 0.01). The results of RT-PCR and Western blotting showed that mRNA and protein levels of STAT3 declined markedly in the treated group. The change in STAT3-related gene expression in tumor tissues at the mRNA and protein level also varied, the expression of survivin, VEGF and c-myc were obviously reduced, and expression of p53 and caspase3 increased (P 〈 0.01). Most of the tumor tissue ceils in the treated group developed apoptosis that was detected by TUNEL assay.CONCLUSION: Silencing of STAT3 expression by RNAi significantly inhibits expression of STAT3 mRNA and protein, and suppresses growth of human HCC in tumor-bearing nude mice. The mechanism may be related to down-regulation of survivin, VEGF and c-myc and up-regulation of p53 and caspase3 expression. Accordingly, the STAT3 gene may act as an important and effective target in gene therapy of HCC. 展开更多
关键词 RNA interference Signal transducerand activator of transcription 3 transcription factor Hepatocellular carcinoma Xenograft model antitumorassays Nude mouse
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