A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 meso...A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.展开更多
In specific condition, when wrapping angle of cold rolling strip covering surface of shape detecting roll dynamically changes, online radial compression of the shape detecting roll is changed too, and it seriously aff...In specific condition, when wrapping angle of cold rolling strip covering surface of shape detecting roll dynamically changes, online radial compression of the shape detecting roll is changed too, and it seriously affects online shape detecting precision of cold strip. Based on the latest intelligent shape meter developed by Yanshan University, using PSO-BP neural network and actual working condition datum, the cold strip online dynamic wrapping angle compensation model is established, and successfully applied in 1250 mm 6-high cold mill, remarkable results are achieved. The error between calculated values and measured values of total tensions is within 3 %展开更多
文摘A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.
基金Item Sponsored by National Science and Technology Support Plan of China(2007BAF02B10)Natural Science Foundation of Hebei Province of China(E2011203004)
文摘In specific condition, when wrapping angle of cold rolling strip covering surface of shape detecting roll dynamically changes, online radial compression of the shape detecting roll is changed too, and it seriously affects online shape detecting precision of cold strip. Based on the latest intelligent shape meter developed by Yanshan University, using PSO-BP neural network and actual working condition datum, the cold strip online dynamic wrapping angle compensation model is established, and successfully applied in 1250 mm 6-high cold mill, remarkable results are achieved. The error between calculated values and measured values of total tensions is within 3 %
