Fabrication, in vitro degradation and cytotoxic assay of different crystalline phases calcium polyphosphate（CPP） were reported. The CPP ceramics were fabricated by crystallizing the amorptous frits, and sintered at ...Fabrication, in vitro degradation and cytotoxic assay of different crystalline phases calcium polyphosphate（CPP） were reported. The CPP ceramics were fabricated by crystallizing the amorptous frits, and sintered at 550℃ ,875℃, 1000℃ for 1 h to obtain the 7-CPP, β-CPP and α-CPP respectively. The efffects of the different crystalline phases on their weight loss and released PO4^3- were investigated during the degradation. And the surface change was observed by the SEM, The osteoblastic ROS17/2.8 cell line was used to estimate the cytotoxicity of CPP. The effects of CPP on cells ＇ proliferation were evaluated by using MTT assay. The experimental restdts showed that γ-CPP, β-CPP and α-CPP did not exert cytotoxic effect on the cells. In addition, the proliferation of the growth of ROS17/2.8 cells on β-CPP was optimal.展开更多
以聚磷酸钙(CPP)为对照,研究掺锶聚磷酸钙(SCPP)微观结构、体外降解产物和速率以及血管内皮细胞相容性,探讨了掺锶对CPP结构性能的影响。结果表明,掺锶改变材料的微观结构与降解性能。与CPP相比,SCPP材料晶粒变大,晶粒间连接更紧密形成...以聚磷酸钙(CPP)为对照,研究掺锶聚磷酸钙(SCPP)微观结构、体外降解产物和速率以及血管内皮细胞相容性,探讨了掺锶对CPP结构性能的影响。结果表明,掺锶改变材料的微观结构与降解性能。与CPP相比,SCPP材料晶粒变大,晶粒间连接更紧密形成平整表面;14 d SCPP降解释放的Ca2+和P i浓度显著下降,即降解速率变慢,降解产物中出现Sr2+,且浓度随时间增加。体外细胞培养结果显示,掺锶能提高材料与血管内皮细胞的相容性。SCPP表面内皮细胞更易粘附、铺展,融合生长形成单层内皮覆盖材料表面。SCPP降解液能显著促进内皮细胞的增殖(p<0.05),经多元逐步回归分析知,降解液中的Sr2+为影响增殖的主要因素(R2=0.670,p<0.01)。SCPP较CPP具有更好的血管内皮细胞相容性,可能促进血管的形成,是更理想的骨组织工程支架材料。展开更多
文摘Fabrication, in vitro degradation and cytotoxic assay of different crystalline phases calcium polyphosphate（CPP） were reported. The CPP ceramics were fabricated by crystallizing the amorptous frits, and sintered at 550℃ ,875℃, 1000℃ for 1 h to obtain the 7-CPP, β-CPP and α-CPP respectively. The efffects of the different crystalline phases on their weight loss and released PO4^3- were investigated during the degradation. And the surface change was observed by the SEM, The osteoblastic ROS17/2.8 cell line was used to estimate the cytotoxicity of CPP. The effects of CPP on cells ＇ proliferation were evaluated by using MTT assay. The experimental restdts showed that γ-CPP, β-CPP and α-CPP did not exert cytotoxic effect on the cells. In addition, the proliferation of the growth of ROS17/2.8 cells on β-CPP was optimal.
文摘以聚磷酸钙(CPP)为对照,研究掺锶聚磷酸钙(SCPP)微观结构、体外降解产物和速率以及血管内皮细胞相容性,探讨了掺锶对CPP结构性能的影响。结果表明,掺锶改变材料的微观结构与降解性能。与CPP相比,SCPP材料晶粒变大,晶粒间连接更紧密形成平整表面;14 d SCPP降解释放的Ca2+和P i浓度显著下降,即降解速率变慢,降解产物中出现Sr2+,且浓度随时间增加。体外细胞培养结果显示,掺锶能提高材料与血管内皮细胞的相容性。SCPP表面内皮细胞更易粘附、铺展,融合生长形成单层内皮覆盖材料表面。SCPP降解液能显著促进内皮细胞的增殖(p<0.05),经多元逐步回归分析知,降解液中的Sr2+为影响增殖的主要因素(R2=0.670,p<0.01)。SCPP较CPP具有更好的血管内皮细胞相容性,可能促进血管的形成,是更理想的骨组织工程支架材料。
