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Preparedness training programs improved the emergency response and crucial outcomes among nursing students:a scoping review
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作者 Budi Mulyana Rian Adi Pamungkas +3 位作者 Widia Sari Anita Sukarno Ernalinda Rosya Wiwik Wariani 《Frontiers of Nursing》 2024年第2期159-167,共9页
Objective:The study aims to review and discuss the outcomes of emergency preparedness training programs among nursing students.Methods:A scoping review was conducted based on the guidelines of Arskey and O’Malley.Dat... Objective:The study aims to review and discuss the outcomes of emergency preparedness training programs among nursing students.Methods:A scoping review was conducted based on the guidelines of Arskey and O’Malley.Databases through PubMed,Science Direct,and Google Scholar were used to obtain the relevant articles within the scoping review.Results:The researchers found 1175 articles.After manual review,28 articles were identified and assessed.The review reported that the emergency preparedness training program significantly affects preparedness,knowledge,performance,self-efficacy,learning motivation,self-confidence,satisfaction,critical decision-making,and problem-solving.Conclusions:Emergency training effectively increases knowledge,skills,confidence,satisfaction,and team performance. 展开更多
关键词 emergency preparedness first aid nursing students training program emergency response disaster management team performance
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扩展目标跟踪Student’s t逆Wishart平滑算法
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作者 陈辉 张丁丁 +1 位作者 连峰 韩崇昭 《电子与信息学报》 EI CAS CSCD 北大核心 2024年第8期3353-3362,共10页
脉冲干扰和离群量测信息等因素通常会导致异常的厚尾噪声,这使得以高斯假设为前提的扩展目标跟踪(ETT)估计器的性能急剧降低,针对该问题该文提出一种基于扩展目标随机矩阵模型(RMM)的Student’s t逆Wishart平滑(StIWS)算法。首先,将目... 脉冲干扰和离群量测信息等因素通常会导致异常的厚尾噪声,这使得以高斯假设为前提的扩展目标跟踪(ETT)估计器的性能急剧降低,针对该问题该文提出一种基于扩展目标随机矩阵模型(RMM)的Student’s t逆Wishart平滑(StIWS)算法。首先,将目标的运动状态以及过程噪声和量测噪声建模为Student’s t分布以表征异常噪声对扩展目标概率分布的影响,将目标扩展状态建模为服从逆Wishart分布的随机矩阵。然后,在Student’s t贝叶斯平滑框架下,详细推导了能在扩展目标的多重特征动态演变的过程中有效估计目标状态的StIWS算法。最后,通过扩展目标跟踪的仿真实验结果和真实场景实验结果验证了所提算法的有效性。 展开更多
关键词 扩展目标跟踪 students t平滑 逆Wishart分布 厚尾噪声
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Analyzing Student Behavior in Online Programming Courses 被引量:1
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作者 Xinyu You Bohong Liu +3 位作者 Menghua Cao Tao Wang Yue Yu Gang Yin 《计算机教育》 2018年第12期48-56,共9页
Rather than maintaining the classic teaching approach, a growing number of schools use the blended learning system in higher education. The traditional method of teaching focuses on the result of students' progres... Rather than maintaining the classic teaching approach, a growing number of schools use the blended learning system in higher education. The traditional method of teaching focuses on the result of students' progress. However, many student activities are recorded by an online programming learning platform at present. In this paper, we focus on student behavior when completing an online open-ended programming task. First, we conduct statistical analysis to examine student behavior on the basis of test times and completed time. By combining these two factors, we then classify student behavior into four types by using k-means algorithm. The results are useful for teachers to enhance their understanding of student learning and for students to know their learning style in depth. The findings are also valuable to re-design the learning platform. 展开更多
关键词 EDUCATIONAL data mining LEARNING analysis student behavior online programMING BLENDED LEARNING environment
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Cross-Cultural Adjustment and Coping:U.S.Students in Taiwan Residents Higher Education
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作者 Wen-Ling Lou 《Sino-US English Teaching》 2024年第1期26-42,共17页
The aim of this study was to investigate the adjustment problems of students from the United States enrolled in universities in the East,specifically in Taiwan,their problems related to cultural adaptation,and the pro... The aim of this study was to investigate the adjustment problems of students from the United States enrolled in universities in the East,specifically in Taiwan,their problems related to cultural adaptation,and the process of adjustment to student life in Taiwan.Under investigation were cultural adjustment and coping skills as these students transitioned from West to East.Qualitative data were collected from interviews with participants and faculty members as well as participant observations.Results indicated that U.S.students found their own ways to acclimate to their new academic setting as well as to social relations,cross-cultural issues,and the linguistic environment in Taiwan to achieve effective adaptation.They made changes in themselves to cope with all situations they encountered.This study provides suggestions for international students abroad in Taiwan,for the Taiwan Residents government,and for universities or colleges in terms of what they should offer to current and future international students. 展开更多
关键词 adjustment cultural adjustment cross-cultural adaptation COPING U.s.students
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Analysis of New Senior English for China Student’s Book 1
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作者 Xia Qian 《语言与文化研究》 2024年第4期128-132,共5页
This article will firstly discuss the context overview of English teaching and learning in China. Under thiscircumstance, a problematic element will be examined of the New Senior English for China Student’s Book 1 an... This article will firstly discuss the context overview of English teaching and learning in China. Under thiscircumstance, a problematic element will be examined of the New Senior English for China Student’s Book 1 and critical appraisal ofhow this element relates to other elements of curriculum development will be analyzed. The recommended changes and demonstrationmechanisms for evaluating the changes are put forward in the last two parts. 展开更多
关键词 New senior English for China students Book 1 Assessment Critical appraisal Curriculum development CHANGEs
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The DNP: Knowledge and perceptions of students in an accelerated master’s program in nursing
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作者 Young-Me Lee Karyn Holm +2 位作者 Elizabeth Florez Megan Glauser Erin Haswell 《Open Journal of Nursing》 2013年第1期138-146,共9页
While the nursing community generally agrees that the Doctorate of Nursing Practice (DNP) degree will strengthen nursing as an academic discipline, there is little known about students’ perceptions of the advanced de... While the nursing community generally agrees that the Doctorate of Nursing Practice (DNP) degree will strengthen nursing as an academic discipline, there is little known about students’ perceptions of the advanced degree. Nursing students enrolled in an accelerated master’s program in nursing (N = 45) were surveyed to assess their knowledge of the DNP degree while also identifying the perceived effect a DNP might have on their careers, on nursing as a discipline, and on public perceptions of nursing practice. In this study, 51% of participants supported the transition to the DNP as the standard degree for practice nursing while 29% were opposed. The majority of participants (71%) planned to pursue an advanced practice nursing degree/certification with 81% of this group signifying that they would do so even if a DNP is required. The majority of participants agreed that the DNP will improve public perception of advanced practice nursing, but 71% thought the title of “doctor” would confuse patients. While current nursing students are generally informed of the upcoming DNP transition, there is disagreement regarding its implications for their careers and for the extent of public understanding. 展开更多
关键词 KNOWLEDGE Perception DNP NURsING students ACCELERATED Master’s program in NURsING
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Effect of Intervention Educational Programs on Improving Quality of Life among Overweight/Obese University Students in Gaza Strip
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作者 Ahmed A. Najim 《Open Journal of Nursing》 2014年第12期886-895,共10页
Background: Overweight and obesity are among the most frequently encountered multi-factorial disorders in most populations of the world. In addition, to its health impacts, overweight/obesity leads to many health prob... Background: Overweight and obesity are among the most frequently encountered multi-factorial disorders in most populations of the world. In addition, to its health impacts, overweight/obesity leads to many health problems which reflect on the university students’ physical, psychological, social and environmental aspects of their quality of life. The study aim was to evaluate the effect of an educational program on improving quality of life of overweight/obese university students in Gaza strip. Materials and Methods: quasi-experimental nonequivalent pretest-posttest control group design utilized in this study 74 and 69 students in the intervention and control group respectively. The study conducted at Islamic university of Gaza and data were collected in two stages before and after five months from the application of the educational program, using interviewing questionnaire and world health organization quality of life-BREF instrument;pre-post test and body mass index were calculated. Results: the study results revealed that overall students’ knowledge for posttest was significantly higher than pretest (p-value ≤ 0.05). Overall, mean score of quality of life after the program was significantly (p-value = 0.001) greater than before and greater than control group for all domains. Conclusion: The study concluded that the health educational intervention program had positive effects on knowledge, practices and attitudes regarding overweight/obesity with improving university students’ mean score of quality of life. 展开更多
关键词 Education program Quality of Life OVERWEIGHT OBEsITY University students
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Influencing program satisfaction for undergraduate students in STEM areas: The impact of interdisciplinary features, disciplinary connectedness, faculty support, and examination difficulty 被引量:1
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作者 Tengteng Zhuang Heng Wang 《教育技术与创新》 2021年第1期14-30,共17页
Ensuring program satisfaction for undergraduate students in the areas of science,technology,engineering and mathematics(STEM)matters in student retention and education quality improvement.This study explores how four ... Ensuring program satisfaction for undergraduate students in the areas of science,technology,engineering and mathematics(STEM)matters in student retention and education quality improvement.This study explores how four rarely examined variables support from faculty members,interdisciplinary features of STEM program courses,disciplinary connectedness of STEM program core courses,and examination difficulty impact Chinese STEM undergraduates’program satisfaction.With data from 619 Chinese STEM undergraduates,structural equation modeling shows that course satisfaction partially mediates the impact of support from faculty members on program satisfaction,while fully mediating that of interdisciplinary features of STEM program courses and disciplinary connectedness of STEM program core courses on program satisfaction.Examination difficulty exerts no significant impact on program satisfaction neither directly nor indirectly.Support from faculty members impact course satisfaction significantly stronger for junior and senior students than for freshmen and sophomores,while interdisciplinary features of STEM program courses impact course satisfaction stronger for freshmen and sophomores than for juniors and seniors.The study ends with practical implications for the higher education reform in relevant areas. 展开更多
关键词 undergraduate sTEM students program satisfaction higher education interdisciplinary and disciplinary features faculty support examination difficulty
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The Influence of Programming Learning on Mathematics Scores of Middle School Students
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作者 Jian Wang Tao Lin +2 位作者 Zhiming Wu Rongsen Zhao Wei Yuan 《计算机教育》 2021年第12期68-73,共6页
Programming teaching plays an important role in improving students’computational thinking and practice.However,due to the fact that programming has not been included in the entrance examination,the rate of having pro... Programming teaching plays an important role in improving students’computational thinking and practice.However,due to the fact that programming has not been included in the entrance examination,the rate of having programming lessons in primary and secondary schools is low.At present,there is still a lack of empirical research on how programming affects the performance of other subjects.This paper studies the influence of programming on students’mathematics achievement for the students of an experimental middle school.The results show that programming has a significant impact on students’math scores,and the students who had not learned code were more likely to show a significant decline in math scores.In addition,learning programming makes it easier for middle-grade students to improve their math scores. 展开更多
关键词 programMING Math score Influence Middle school student
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Appeal of 3 + 4 Program: Good Students are Few but Growing at Secondary Vocational Schools in Shanghai
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作者 XU Hui XIE Peng-yang 《Sino-US English Teaching》 2018年第6期278-283,共6页
Secondary vocational education in Shanghai continues to experiment in an attempt to find ways to solve the decline of student enrollment and adjust to rapidly changing social and economic environments. The 3 + 4 Pilo... Secondary vocational education in Shanghai continues to experiment in an attempt to find ways to solve the decline of student enrollment and adjust to rapidly changing social and economic environments. The 3 + 4 Pilot Program (three-year secondary vocational education and four-year undergraduate education) has been under experiment since 2014. It has attracted more and more academically good junior middle school graduates to secondary vocational schools with the national and local favorable policies and laws. Except the three-year’s hand-on practice, what appeals most to the junior middle school graduates is the academic transfer under the Program, a breakthrough in history of vocation education in China. However, only initiating a new program is not enough. Shanghai government should map out a long-term plan to set up promotion to increase the public awareness to change the long prevailing outdated ideology in order to add more to its appeal of the Program. There is still much work to be done before expanding the scope of the Program. 展开更多
关键词 APPEAL Pilot program student enrollment secondary vocational schools
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A Case Study of Intercultural Interventions for Chinese University Students During Study Abroad Program in the University of Minnesota
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作者 WANG Ju-lan 《Journal of Literature and Art Studies》 2018年第3期457-464,共8页
In 21st Century, Chinese and American universities are experiencing great development in the number of students studying abroad. This research adopts the method of natural investigation, selecting Chinese student samp... In 21st Century, Chinese and American universities are experiencing great development in the number of students studying abroad. This research adopts the method of natural investigation, selecting Chinese student samples during study abroad program in the University of Minnesota to complete the designed questionnaires to examine the intercultural interventions taken before, during and after this type of study abroad in Chinese universities. Also, after students complete the questionnaires, email communication is made to analyze the reasons for their answers to the questionnaires. The questionnaire results and email communication show that there is a great gap in the practice of intercultural interventions in study abroad programs between Chinese universities and students expectations. It is time for Chinese universities to design appropriate intercultural interventions in order to maximize Chinese university students' intercultural development during study abroad. These intercultural interventions should be run consistently to cover the phrases of pre-departure, during and after the study abroad program. Also, more dynamic and interactive intercultural interventions rather than knowledge-based intercultural instructions or guidance should be the focus for intercultural interventions. 展开更多
关键词 intercultural interventions study abroad program intercultural competence Chinese university students
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An effective Australian-Chinese peer education program on HIV/AIDS/STDs for university students in China
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作者 高源 施榕 +3 位作者 吕姿之 仇玉兰 钮文异 Short RV 《生殖医学杂志》 CAS 2002年第z1期7-12,共6页
  IntroductionCurrently, there are at least 850,000 people living with human immuno-deficiency virus (HIV) and acquired immuno-deficiency syndrome (AIDS) in China. Among them 16-29 years old account for about 65%. A...   IntroductionCurrently, there are at least 850,000 people living with human immuno-deficiency virus (HIV) and acquired immuno-deficiency syndrome (AIDS) in China. Among them 16-29 years old account for about 65%. According to the Chinese health authorities, if the preventive measures are not effective, the figure could reach as many as 10 million by the year 2010[1]. Since there are currently no cure or vaccines for HIV/AIDS, changing people's high-risk behaviors is the main approach that is taken to slow down the epidemic. This can be done through effective HIV/AIDS education, especially for young people. In 1997, a 4-year adolescent HIV/AIDS peer education program was introduced to in Beijing and Shanghai, which was based on a successful Australian model developed by Professor Roger Short, in which senior medical students were trained and used as peer educators to teach their junior peers about HIV/AIDS[2].…… 展开更多
关键词 AIDs sTD HIV An effective Australian-Chinese peer education program on HIV/AIDs/sTDs for university students in China
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Strangers in a New Land: Rural School Personnel’s Perceptions of the Implementation of an International Student Program
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作者 Caryn A Lasky 《Journal of International Education and Practice》 2019年第2期24-36,共13页
Many high schools in the northeastern United States have suffered from declining enrollment due to a declining population of school-age children in the region.Administrators,counselors and teachers perceive many impac... Many high schools in the northeastern United States have suffered from declining enrollment due to a declining population of school-age children in the region.Administrators,counselors and teachers perceive many impacts of the implementation of an international student program at a rural high school.This qualitative case study reveals that the rural high school’s unique nature impacts international student programs,international students influence both school culture and programming,and international students suffer from isolation.School leaders would benefit from considerations in the form of professional development both prior to implementation of an international student program and ongoing throughout its duration.It is important for schools to find ways to respectfully honor student cultures and optimize learning experiences.School leaders would be wise to determine desired size of international student programs and make efforts accordingly to achieve that size for optimal effectiveness for the benefit of both host and international students.Implications and recommendations for future research include further exploration of the perceived experiences of European and Chinese international students,understanding the unique nature of rural high schools and international student experiences,and examination of school practices. 展开更多
关键词 International student program school Personnel Perceptions Rural High schools Host Group Interactive Acculturation Model
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Level of Insomnia, Symptoms of Depression, and Anxiety among College Students with Mobile Phone Addiction: Basis for Guidance and Counseling Program Enhancement
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作者 Xiaowei He Elna Lopez 《Open Journal of Applied Sciences》 2023年第10期1641-1648,共8页
Mobile phone addiction is a growing concern among college students, with significant implications for their mental health. This study examines the complex relationship between mobile phone addiction and its impact on ... Mobile phone addiction is a growing concern among college students, with significant implications for their mental health. This study examines the complex relationship between mobile phone addiction and its impact on college students’ insomnia, symptoms of depression, and anxiety. A sample of 399 college students from traditional Chinese medicine colleges in Guangxi, China, completed the Mobile Phone Addiction Tendency Scale questionnaire. Among them, 155 students with high Mobile Phone Addiction Tendency scores were selected for the correlation study. The analysis revealed a strong association between mobile phone addiction and insomnia. Furthermore, mobile phone addiction was significantly associated with symptoms of depression, including feelings of sadness, worthlessness, and hopelessness, as well as heightened anxiety symptoms, such as excessive worry and restlessness. These findings emphasized the importance of targeted interventions and preventive measures to address the negative impact of mobile phone addiction on college students’ mental health. 展开更多
关键词 College students Mobile Phone Addiction INsOMNIA symptoms of Depression ANXIETY Mental Health Intervention program
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Correlates of the School-Based Guidance Program to Freshman Students in Guangdong Province,China:Basis for Enhanced Guidance Program
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作者 Cijiang Xiong 《Journal of Contemporary Educational Research》 2022年第9期53-64,共12页
Guidance programs have significant roles in preparing students for the future.The study recognizes that guidance and counseling programs are a transformative process of helping students strengthen their career plannin... Guidance programs have significant roles in preparing students for the future.The study recognizes that guidance and counseling programs are a transformative process of helping students strengthen their career planning,education,and preparation for employment.The guidance programs of first-year college students from Guangdong Peizheng College(GPC)in Guangdong Province,China was investigated,with 357 freshman students currently enrolled in the participating school involved in this study.Moreover,12 guidance counselors were interviewed through focused-group discussions,including the school director,for the qualitative component of the study.Utilizing the convergent parallel design mixed method,the study was able to elucidate the quantitative and qualitative relevance of the data gathered from the selected participants.The emerging results found that Chinese freshman students were satisfied with all of the parameters of the guidance programs of the school.The school counselors are able to provide students with a rich reservoir of academic cand guidance support,career mentoring,and career guidance.There were contributing factors that need to be addressed if improvement and innovation of the guidance program is the primary goal.Support and resources should be prioritized in order to enrich the development not only of the students but also of the guidance counselors.The study serves as empirical evidence for proposing an improved school-based guidance program as practical outputs of the study further to innovate the effectiveness of the implementation of guidance services. 展开更多
关键词 Guidance program Career development Counselors Freshman students
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厚尾噪声条件下星凸形扩展目标student’s t滤波器 被引量:2
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作者 陈辉 张星星 杨文瑜 《兰州理工大学学报》 CAS 北大核心 2021年第5期85-92,共8页
针对非线性非高斯厚尾噪声条件下的不规则形状的扩展目标跟踪问题,提出了基于随机超曲面模型(RHM)的星凸形扩展目标student’s t滤波算法.首先,在带有非高斯厚尾过程噪声和厚尾量测噪声的系统中,基于student’s t分布给出鲁棒student’... 针对非线性非高斯厚尾噪声条件下的不规则形状的扩展目标跟踪问题,提出了基于随机超曲面模型(RHM)的星凸形扩展目标student’s t滤波算法.首先,在带有非高斯厚尾过程噪声和厚尾量测噪声的系统中,基于student’s t分布给出鲁棒student’s t滤波器.其次,利用随机超曲面模型描述任意星凸形扩展目标的量测源分布,提出带厚尾噪声的星凸形扩展目标student’s t滤波器.最后通过仿真验证了所提方法的正确性和有效性. 展开更多
关键词 扩展目标跟踪 随机超曲面模型 鲁棒students t滤波器 students t分布
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基于数据流的K-S变化检测的动态多目标规划算法 被引量:1
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作者 张涛 周晨 +2 位作者 杜锋 陈芳 刘瑞林 《长江大学学报(自然科学版)》 2024年第1期109-116,共8页
为了更加准确地判断环境是否发生变化并快速追踪动态多目标规划问题(dynamicmulti-objectiveoptimization problem,DMOP)当前时刻的Pareto前沿,提出了一种基于数据流的Kolmogorov-Smirnov(K-S)变化检测的动态多目标规划(DSK-SDMOP)算法... 为了更加准确地判断环境是否发生变化并快速追踪动态多目标规划问题(dynamicmulti-objectiveoptimization problem,DMOP)当前时刻的Pareto前沿,提出了一种基于数据流的Kolmogorov-Smirnov(K-S)变化检测的动态多目标规划(DSK-SDMOP)算法。该算法以NSGA-Ⅱ为基础,通过数据流建立2个时刻的检验窗口,再利用K-S检验基于数据流的Pareto最优前沿是否发生变化,检测2个窗口的数据是否服从同一分布来判断环境是否发生变化,并就环境变化的剧烈程度实行相应的应答机制,以提高对环境的适应程度。利用基于数据流的K-S检测方法,对环境变化不会过于敏感,而且不用提前假设对应目标值的分布,易于操作。通过5个动态多目标规划标准测试函数对该算法进行测试,并和现有的2种算法进行对比分析,结果表明该算法处理动态多目标规划问题具有良好的性能。 展开更多
关键词 动态多目标规划 数据流 K-s检验 NsGA-Ⅱ
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A Research on Middle School Students'English Reading Obstacles and Strategies
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作者 杨维玱 《海外英语》 2012年第15期43-46,共4页
English reading,a key procedure of English learning and teaching in middle school,is an essential way of getting information from the globe and communicating with others.Therefore,efficient comprehension on reading ma... English reading,a key procedure of English learning and teaching in middle school,is an essential way of getting information from the globe and communicating with others.Therefore,efficient comprehension on reading materials will directly serve for accepting the information.And it is extremely important for middle school students to acquire certain reading ability.At present,great attention has been paid to the course of reading in the instruction of English by the Chinese Education Department.But middle school students'read ing ability is still much lower than the target they should reach because of a set of existing obstacles that prevent them from being effective English readers.What are these obstacles? How to break them down? This paper will first point out and then analyze the obstacles that af fect middle school students'reading ability from two aspects:linguistic obstacles(obstacles of lexicon,grammar and background knowl edge) and non-linguistic obstacles(obstacles of psychology,interest,skill,and habit).After demonstrating different obstacles,the paper also come up with some corresponding strategies in the hope of helping middle school students to be effective English readers. 展开更多
关键词 English READING MIDDLE sCHOOL students obstacles s
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基于MRI影像组学构建PD-1/PD-L1抑制剂治疗dMMR/MSI-H直肠癌疗效的预测模型
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作者 张岚 周彦汝 +3 位作者 韩鼎盛 张嘉诚 何旭 刘鹏 《中国医学计算机成像杂志》 CSCD 北大核心 2024年第3期343-348,共6页
目的:探讨MRI影像组学模型在程序性细胞死亡蛋白-1(PD-1)/程序性细胞死亡-配体1(PD-L1)抑制剂联合全程新辅助治疗(TNT)局部进展期直肠癌(LARC)的疗效预测价值。方法:收集河南中医药大学第一附属医院PD-1/PD-L1抑制剂联合TNT治疗的80例... 目的:探讨MRI影像组学模型在程序性细胞死亡蛋白-1(PD-1)/程序性细胞死亡-配体1(PD-L1)抑制剂联合全程新辅助治疗(TNT)局部进展期直肠癌(LARC)的疗效预测价值。方法:收集河南中医药大学第一附属医院PD-1/PD-L1抑制剂联合TNT治疗的80例错配修复基因缺陷(dMMR)/微卫星高度不稳定(MSI-H)基因型中低位LARC患者的临床和影像资料。将入组患者按7∶3比例分为训练集和测试集,提取影像组学特征,从中筛选并构建影像组学模型。描绘影像组学模型的Rad-score与病理金标准之间的受试者工作特征(ROC)曲线,计算曲线下面积(AUC),并评价模型的诊断效能。采用决策曲线分析(DCA)计算风险阈值的范围,并评估临床获益情况。收集湖南省人民医院25例dMMR/MSI-H基因型LARC患者的影像资料作为外部验证集。结果:训练集、测试集及外部验证集三者之间的临床特征无统计学差异(P>0.05)。经过降维处理、t检验及一致性检验以及LASSO交叉验证后,筛选出一阶偏度特征和体积2个特征构建影像组学模型。训练集、测试集和外部验证集的影像组学预测模型ROC曲线的AUC、灵敏度、特异度、阳性预测值和阴性预测值分别为0.920、97.1%、85.7%、91.9%、94.7%;0.885、80.0%、88.9%、92.3%、72.7%;0.875、87.5%、88.9%、93.3%、80.0%。DCA曲线显示,当风险阈值范围为0%~82%时,采用影像组学模型预测LARC患者为病理完全缓解(pCR)的获益大于将所有患者都视为pCR或者无病理完全缓解(npCR)。结论:基于MRI影像组学构建的dMMR/MSI-H型局部进展期直肠癌PD-1/PD-L1抑制剂联合全程新辅助放化疗疗效预测模型,有较大潜力为不同基因分型的直肠癌患者制定个体化治疗策略提供量化依据。 展开更多
关键词 磁共振成像 影像组学 直肠肿瘤 局部进展期 程序性细胞死亡蛋白-1/程序性细胞死亡-配体1 全程新辅助放化疗
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S-烯丙基巯基半胱氨酸促进CD8+T细胞杀伤功能的机制研究
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作者 翟健 李军 《中国耳鼻咽喉头颈外科》 CSCD 2024年第2期74-78,共5页
目的探究S-烯丙基巯基半胱氨酸(S-allylmercaptocysteine,SAMC)对CD8^(+)T细胞杀伤鼻咽癌细胞功能的影响及机制。方法将人鼻咽癌细胞HK-1和C666-1与SAMC共培养,分为0、25、50、100μmol/L SAMC组,Western blot检测肿瘤细胞程序性死亡配... 目的探究S-烯丙基巯基半胱氨酸(S-allylmercaptocysteine,SAMC)对CD8^(+)T细胞杀伤鼻咽癌细胞功能的影响及机制。方法将人鼻咽癌细胞HK-1和C666-1与SAMC共培养,分为0、25、50、100μmol/L SAMC组,Western blot检测肿瘤细胞程序性死亡配体-1(programmed cell death-ligand 1,PD-L1)表达。CD8^(+)T细胞分别与HK-1和C666-1细胞以10∶1的比例共培养并加入0、25、50、100μmol/L SAMC,检测CD8^(+)T对HK-1和C666-1的杀伤能力,酶联免疫法(ELISA)检测干扰素(INF-γ)和肿瘤坏死因子-α(TNF-α)浓度,构建鼻咽细胞HK-1的小鼠皮下移植瘤模型,分为对照组、CD8^(+)T细胞组、SAMC组、SAMC+CD8^(+)T细胞组,各组小鼠治疗期间测量瘤体积,治疗结束后取小鼠肿瘤组织,Western blot检测肿瘤组织中PD-L1表达,ELISA检测小鼠血清INF-γ和TNF-α浓度。结果相比于0μmol/L SAMC组,25、50、100μmol/L SAMC组HK-1和C666-1细胞PD-L1表达均显著下调(P<0.05),相比于0μmol/L SAMC+CD8^(+)T细胞组,25、50、100μmol/L SAMC+CD8^(+)T细胞组HK-1和C666-1细胞培养上清中INF-γ和TNF-α浓度均能显著增加,HK-1和C666-1细胞裂解率显著增加(P<0.01)。相比于对照组,CD8^(+)T细胞组和SAMC+CD8^(+)T细胞组小鼠瘤体积和瘤重显著下降(P<0.05),小鼠血清INF-γ和TNF-α浓度显著增加,相比于CD8^(+)T细胞组,SAMC+CD8^(+)T细胞组小鼠瘤体积和瘤重显著下降(P<0.05),小鼠血清INF-γ和TNF-α浓度显著增加(P<0.05),肿瘤组织PD-L1表达显著下调(P<0.01)。结论SAMC可通过抑制人鼻咽癌细胞PD-L1表达而促进CD8^(+)T细胞的杀伤功能。 展开更多
关键词 鼻咽癌 酶联免疫吸附测定 s-烯丙基巯基半胱氨酸 细胞程序性死亡配体-1
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