Potential Impacts of Exogenous Nitrogen Enrichment on Distribution and Transfer of Nitrogen in Plant-Soil System of Suaeda salsa Marsh in the Yellow River Estuary, China

To determine the potential impacts of exogenous nitrogen(N)enrichment on distribution and transfer of N in Suaeda salsa marsh in the Yellow River Estuary,the variations of N in plant-soil system during the growing season were investigated by field N addition experiment.The experiment included four treatments:NN(no N input treatment,0gNm^(−2) yr^(−1)),LN(low N input treatment,3.0 gNm^(−2) yr^(−1)),MN(medium N input treatment,6 gNm^(−2) yr^(−1))and HN(high N input treatment,12 gNm^(−2) yr^(−1)).Results showed that N additions generally increased the contents of total nitrogen(TN),ammonia nitrogen(NH_(4)^(+)-N)and nitrate nitrogen(NO_(3)^(−)-N)in different soil layers and the increasing trend was particularly evident in topsoil.Compared with the NN treatment,the average contents of TN in topsoil in the LN,MN and HN treatments during the growing season increased by 10.85%,30.14%and 43.98%,the mean contents of NH_(4)^(+)-N increased by 8.56%,6.96%and 14.34%,and the average contents of NO_(3)^(−)-N increased by 35.73%,45.99%and 46.66%,respectively.Although exogenous N import did not alter the temporal variation patterns of TN contents in organs,the N transfer and accumulation differed among tissues in different treatments.With increasing N import,both the N stocks in soil and plant showed increasing trend and the values in N addition treatments increased by 9.43%–38.22%and 13.40%–62.20%,respectively.It was worth noting that,compared with other treatments,the S.salsa in the MN treatments was very likely to have special response to N enrichment since not only the period of peak growth was prolonged by about 20 days but also the maximum of TN content in leaves was advanced by approximately one month.This paper found that,as N loading reached MN level in future,the growth rhythm of S.salsa and the accumulation and transference of N in its tissues would be altered significantly,which might generate great impact on the stability and health of S.salsa marsh ecosystem.

水盐环境梯度下翅碱蓬(Suaeda salsa)的生态阈值

在对翅碱蓬(Suaeda salsa)生物量、密度、株高、盖度、多度相关性分析的基础上选取了生物量作为翅碱蓬生物指标,利用高斯模型分析了黄河三角洲翅碱蓬种群沿水深、土壤盐分的生态阈值,翅碱蓬最适水深为-0.42m,水深生态阈值区间为[-0.92,0.08](m),水深最适生态阈值区间为[-0.67,-0.17](m);最适土壤盐分为12.71 g/kg左右,其盐分生态阈值区间为[5.17,20.25](g/kg),盐分最适生态阈值区间为[8.94,16.48](g/kg)。通过分析不同实验区的水盐关系及其交互作用,探讨了水盐交互作用对翅碱蓬生长的影响。最后,通过离差平方和聚类分析,将3个实验区69个样地划分为7类。随着水深和盐分的梯度变化,7类样地的翅碱蓬群落呈现明显的演替。

碱蓬(Suaeda salsa)对胶州湾滨海湿地重金属的富集与迁移作用

通过对胶州湾东北部滨海湿地5个采样点生长的碱蓬中10种重金属Cu、Zn、Pb、Cd、As、Cr、Co、Ni、V、Mo的系统研究,发现碱蓬对Cu,Zn的吸收明显高于其它重金属,这可能与Cu和Zn是植物生长发育必需的微量元素有关,碱蓬体内Cr、Pb、V和Ni的含量也相对较高。碱蓬对Mo的富集效果最为显著,对Cu、Zn、Cd和As也表现出一定的富集作用,而对其它重金属的富集效果不明显。碱蓬的根、茎和叶对重金属的富集系数从大到小依次为:Cd>As>Cu>Zn>Mo>Co>Ni>V>Pb>Cr;Mo>Cd>Zn>Cu>As,Co,Ni>Pb,Cr>V;Mo>Cd>Zn>Cu>As>Ni>Co>Pb,Cr>V,总体而言,碱蓬对Mo和Zn的迁移效率较高,大部分被迁移至地上部分,输送到茎叶的平均相对含量分别占66.3%和65.9%,其次是Cu、Pb、Cd、Cr、Co和Ni,它们在碱蓬地上和地下部分的比例大致相当,而碱蓬对As和V的迁移效率最低,输送到茎叶的平均相对含量分别只有33.5%和37.2%。

盐地碱蓬 (Suaeda salsa)中SsINPS基因的分子克隆及表达特性分析(英文)

肌醇 1 磷酸 (I 1 P)合成酶 (EC5 .5 .1 .4,INPS)是肌醇生物合成中的关键酶 ,催化葡萄糖 6 磷酸 (G 6 P)到I 1 P的反应。从该实验室已构建的NaCl40 0mmol/L处理的盐地碱蓬 (Suaedasal sa)cDNA文库中克隆了肌醇 1 磷酸合成酶的全长cDNA (S .salsamyo inositol 1 phosphatesynthase,SsINPS) ,基因注册号为AF43 3 879。SsINPS全长约 1 986bp ,含有开放式阅读框架 1 5 3 0bp ,3′和 5′的非翻译区分别为 1 3 9bp和 3 1 7bp ;推导的氨基酸序列全长 5 1 0个氨基酸残基 ,分子量约为 5 6 .7kD ,pI值为 5 .3 5。BLAST同源性分析表明 ,该cDNA与已报告的冰叶日中花 (Mesembryanthemumcrys tallinum)的INPS基因同源性最高 ,其中 ,核苷酸水平的同源性为 91 % ,氨基酸水平上的同源性为84%。以SsINPS全长cDNA为探针进行的South ern杂交结果表明 ,SsINPS基因在盐地碱蓬基因组中只有一个拷贝 ;Northern结果表明 ,在盐处理(40 0mmol/L的NaCl)下 ,SsINPS在叶中的表达量有显著的增加。

胶州湾滨海湿地中的Li、Rb、Cs、Sr、Ba及碱蓬(Suaeda salsa)对其的“重力分馏”

通过对胶州湾东北部滨海湿地5个站点盐渍土壤和其上生长的碱蓬中的微量碱金属、碱土金属Li、Rb、Cs、Sr、Ba的系统研究,发现盐渍土壤中Li、Sr和Ba的含量高于我国土壤背景值,Rb、Cs的含量特别是Cs的含量低于我国土壤的背景含量,盐渍土壤中Li/Cs比值和Ba/Sr比值较为接近,并且Li和Cs、Sr和Ba之间具有显著的正相关关系,表明这一区域的盐渍土壤有相似的成土母质。碱蓬对碱土金属的积累量明显高于碱金属,但对它们的富集系数都小于1,碱蓬生物富集系数Sr>Li>Rb>Cs>Ba,碱蓬对这五种元素从盐渍土壤中迁出的能力随着它们原子序数的增加明显降低,即越重的元素越不易被迁离地面移出盐渍土壤,相反,越轻的元素越易被迁离地面移出盐渍土壤,碱蓬可造成盐渍土壤Li、Rb、Sr、Cs、Ba明显的"重力分馏"。

Fertilization Affects Biomass Production of Suaeda salsa and Soil Organic Carbon Pool in East Coastal Region of China

Land use practice significantly affects soil properties. Soil is a major sink for atmospheric carbon, and soil organic carbon （SOC） is considered as an essential indicator of soil quality. The objective of this study was to assess the effects of N and P applied to Suaeda salsa on biomass production, SOC concentration, labile organic carbon （LOC） concentration, SOC pool and carbon management index （CMI） as well as the effect of the land use practice on soil quality of coastal tidal lands in east coastal region of China. The study provided relevant references for coastal exploitation, tidal land management and related study in other countries and regions. The field experiment was laid out in a randomized complete block design, consisting of four N-fertilization rates （0 （NO）, 60 （N1）, 120 （N2） and 180 kg ha-1 （N3））, three P-fertilization rates （0 （P0）, 70 （P1） and 105 kg ha-~ （P2）） and bare land without vegetation. N and P applied to S. salsa on coastal tidal lands significantly affected biomass production （above-ground biomass and roots）, bulk density （Pb）, available N and P, SOC, LOC, SOC pool and CMI. Using statistical analysis, significantly interactions in N and P were observed for biomass production and the dominant factor for S. salsa production was N in continuous 2-yr experiments. There were no significant interactions between N and P for SOC concentration, LOC concentration and SOC pool. However, significant interaction was obtained for CMI at the 0-20 cm depth and N played a dominant role in the variation of CMI. There were significant improvements for soil measured attributes and parameters, which suggested that increasing the rates of N and P significantly decreased Pb at the 0-20 cm depth and increased available N and P, SOC, LOC, SOC pool as well as CMI at both the 0-20 and 20-40 cm depth, respectively. By correlation analysis, there were significantly positive correlations between biomass （above- ground biomass and roots） and SOC as well as LOC in 2010 and 2011 across all soil depth, respectively. The treatment with N at 180 kg ha-~ and P at 105 kg ha-1 was superior to the other treatments. The results from the 2-yr continuous experiments indicated that, in short-term, there were a few accumulation of SOC and LOC concentrations by means of N and P application to S. salsa, whereas in the long run, S. salsa with N and P application was recommended for coastal tidal lands because of its great potential of carbon sequestration, improvements of soil nutrition status and promotion of soil quality.

Growth and sustainability of Suaeda salsa in the Lop Nur,China

Extremely saline soils are very harsh environments for the growth and survival of most plant species, however, halophytes can grow well. The underlying mechanism of halophyte to resist high saline is not well understood by us. This study was conducted at the potash mine near the Lop Nur, China, where the effects of the halophyte Suaeda salsa L. on the saline-alkaline soils and its growth and sustainability were investigated. Four plots（in which the salt encrustation layers were removed）, with different soil treatments were evaluated：（1） undisturbed soil, with no additional treatment（T1）;（2） the slag soil zone, in which a 40-cm layer of slag was placed on the undisturbed soil surface（T2）;（3） slag＋sandy soil, in which a 20-cm layer of slag was placed in the lower layer and 20 cm of sandy soil, taken from an area about 70 km away from Lop Nur potash mine, where Tamarix species were growing, was placed in the upper layer（T3）; and（4） a 40-cm sandy soil layer taken from the area where Tamarix species were growing was placed on undisturbed soil（T4）. Soil nutrient contents increased in the four treatments, but salt content only decreased in the T1 treatment. Salt content in the T4 treatment increased over the two-year period, which may be partly attributed to salt deposition from wind-blown dust within the mine and salt accumulation within the surface soil（0–20 cm） in response to high evaporative demands. The S. salsa plants exhibited greater improvements in growth under the T4 treatment than under the T1, T2, and T3 treatments, which demonstrated that low levels of salinity are beneficial for the growth of this species. The T1 treatment was sustainable because of its low cost and superior soil improvement characteristics. Therefore, S. salsa plants not only reduced soil salinity and increased soil nutrient levels, but also ameliorated the plant growth environment, which would be beneficial for both the ecological restoration of the Lop Nur area and similar areas throughout the world.

Influence of Artificial Cofferdam and Spartina alterniflora Expansion on Evolution of Suaeda salsa Marsh in Yancheng Coastal Wetland of East China

Suaeda salsa is an important local species in the intertidal beach of the Western Pacific coast. However,under the artificial cofferdam and Spartina alterniflora expansion,Suaeda marsh has degraded seriously. Therefore,using Yancheng Nature Reserve as a case study area,taking ETM+images in 2000,2006 and 2011 as the basic data sources,we revealed the evolution characteristics of Salsa marsh which was impacted. The research results are as follows. From 2000 to 2011,Salsa marsh area in the artificial area tempestuously degraded,decreasing by 87. 158%,more than 22% than those in the natural area. The landscape was fragmentized. Landscape polymerization degree index dropped from 95. 780 to 65. 455,more than 16% than those in the natural area. The mean patch area fell down to 21. 429 ha from 389. 333 ha,more than 11% that in the natural area. Compared to the steady change in natural conditions,the area was reduced by 118. 167 ha/a from 2000 to 2006,while during 2006-2011,it was only 51. 500 ha/a in artificial area. As for spatial change of landscape,in artificial area,the Salsa marsh centroid moved forward to the southeast with 666. 350 m,but that in natural area moved forward to the north with 1 042. 710 m from 2000 to 2006. From 2006 to 2011,the centroid moved forward to east and north respectively. Artificial cofferdam transformed the area into freshwater ecosystem,and meanwhile the freshwater was beneficial to Reed marsh. During 2000 to 2006,in the artificial area,539 ha Salsa marsh controlled by cofferdam transferred into reed marsh and aquaculture ponds,of which the transformation rate was nearly 4% higher than that in natural area. From 2006 to 2011,178 ha Salsa marsh was transferred into reed marsh,the transformation rate was 20% higher than that in natural area. With rapid spreading and strong competition of Spartina species,the coastal wetland has formed the pattern of " Salsa – Spartina marshes". From 2000 to 2006,in artificial area,15. 24% of Salsa marsh was transferred into Spartina marsh,of which the transformation rate was13% higher than that in natural area. And from 2006 to 2011,30. 07% Salsa marsh was replaced by the Spartina marsh in artificial area,the rate was almost 10% higher than that in the natural area.

Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa

The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity.Since phytochelatins can effectively chelate heavy metals,it was hypothesized that S.salsa possessed a phytochelatin synthase(PCS) gene.In the present study,the cDNA of PCS was obtained from S.salsa(designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends(RACE).A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides,encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain.A similarity analysis suggested that SsPCS shared up to a 58.6%identity with other PCS proteins and clustered with PCS proteins from eudicots.There was a new kind of metal ion sensor motif in its C-terminal domain.The SsPCS transcript was more highly expressed in elongated and fibered roots and stems(P<0.05) than in leaves.Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS(P<0.05).To the best of our knowledge,SsPCS is the second PCS gene cloned from a halophyte,and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila.This study provided a new view of halophyte PCS genes in heavy metal tolerance.

滴灌条件下盐地碱蓬(Suaeda salsa)种植年限对盐碱地土壤盐分离子分布的影响

采用时空转化的方法研究了滴灌条件下不同盐地碱蓬种植年限(0 a、1 a、2 a和3 a)对重度盐碱地土壤盐分及盐离子在0~120 cm土壤剖面的分布特征的影响,为盐地碱蓬在盐碱地中的改良利用提供理论依据。试验结果表明:滴灌种植盐地碱蓬后土壤盐分在剖面的分布发生明显变化,根区(0~40 cm)土壤含盐量随种植年限增加而下降,根底(40~120 cm)土壤则先增加后下降;Na+和Cl-因容易被水淋洗和植物选择性吸收多,根区土壤中Na+和Cl-含量随种植年限增加有显著降低,Ca2+和SO42-不易随水移动,淋洗程度低,HCO3-和Mg2+第2 a和第3 a的淋洗效果明显好于第1 a;经过3 a种植后土壤中毒害离子Na+与Cl-和在表层盐分组成中的比例下降,Ca2+比例上升,钠吸附比(SAR)值显著降低。

Changes of Biogenic Elements in Phragmites australis and Suaeda salsa from Salt Marshes in Yellow River Delta,China

Little information is available on biogenic elements(carbon, nitrogen, phosphorus and sulfur) and the ecological stoichiometric characteristics of plants in coastal wetlands. To investigate the contents of carbon, nitrogen, phosphorus and sulfur of plants, and their ecological stoichiometric characteristics in the Yellow(Huanghe) River Delta, plant samples were collected from two typical salt marshes(Suaeda salsa and Phragmites australis wetlands) during the period of from August to October in 2007, and the ratios of C/N, C/P, N/P, C/N/P and C/N/P/S were calculated. Results showed that during the studying period, plant C, N and P were lower than the global average values, and plant N and P were lower than the China's average values. Leaf C and S in Suaeda salsa were significantly lower than those in Phragmites australis(P < 0.05), and leaf N and P in Suaeda salsa and Phragmites australis showed no significant differences(P > 0.05). Average C/N ratios were 23.75 in leaf, 73.36 in stem, 65.67 in root of Suaeda salsa, and 33.77 in leaf, 121.68 in stem, 97.13 in root of Phragmites australis. Average C/N ratios of Suaeda salsa and Phragmites australis were all great than 25, indicating the salt marsh in the Yellow River Delta is an N limitation system. Average C/P ratios were 276.78 in leaf, 709.28 in stem and 1031.32 in root of Suaeda salsa, and 536.94 in leaf, 768.13 in stem and 875.22 in root of Phragmites australis. The average N/P ratios of Suaeda salsa were 12.92 in leaf, 10.77 in stem and 10.91 in root, and the average N/P ratios of Phragmites australis were 16.40 in leaf, 7.40 in stem and 6.92 in root, indicating the Suaeda salsa wetlands were N limited and Phragmites australis wetlands were N limited in August and P limited in October in 2007. The average C/N, C/P and C/N/P ratios in Suaeda salsa and Pragmites australis were higher than the global average values, indicating the lower quality of organic matter provided by wetland plants in the Yellow River delta.

盐地碱蓬(Suaedasalsa)APX基因的克隆及盐胁迫下的表达

从盐地碱蓬 (Suaedasalsa)中克隆了抗坏血酸过氧化物酶 (ascorbateperoxidase ,APX)的全长cDNA(SsAPX) ,基因注册号为AY0 34 893。SsAPX全长 1.1kb ,推导的氨基酸序列长为 2 5 0个氨基酸残基。BLAST同源性分析表明 ,该cDNA与已报告的菠菜(Spinaciaoleracea)细胞质抗坏血酸过氧化物酶基因同源性最高 ,在核苷酸水平上一致性为 87% ,在氨基酸水平上一致性为 89%。Southern杂交表明APX基因在盐地碱蓬基因组中只有 1个拷贝。盐 (NaCl 40 0mmol/L)处理不同时间后的Northern杂交分析表明盐地碱蓬中SsAPX基因在盐胁迫下表达量增加 ,而且在盐胁迫下抗坏血酸过氧化物酶的活性也显著地增加 ,说明该基因受盐诱导。

Nitrogen cycle of a typical Suaeda salsa marsh ecosystem in the Yellow River estuary

The nitrogen（N） biological cycle of the Suaeda salsa marsh ecosystem in the Yellow River estuary was studied during 2008 to 2009.Results showed that soil N had significant seasonal fluctuations and vertical distribution.The N/P ratio（15.73±1.77） of S.salsa was less than 16,indicating that plant growth was limited by both N and P.The N absorption coefficient of S.salsa was very low（0.007）,while the N utilization and cycle coefficients were high（0.824 and 0.331,respectively）.The N turnover among compartments of S.salsa marsh showed that N uptake from aboveground parts and roots were 2.539 and 0.622 g/m2,respectively.The N translocation from aboveground parts to roots and from roots to soil were 2.042 and 0.076 g/m2,respectively.The N translocation from aboveground living bodies to litter was 0.497 g/m2,the annual N return from litter to soil was far less than 0.368 g/m2,and the net N mineralization in topsoil during the growing season was 0.033 g/m2.N was an important limiting factor in S.salsa marsh,and the ecosystem was classified as unstable and vulnerable.S.salsa was seemingly well adapted to the low-nutrient status and vulnerable habitat,and the nutrient enrichment due to N import from the Yellow River estuary would be a potential threat to the S.salsa marsh.Excessive nutrient loading might favor invasive species and induce severe long-term degradation of the ecosystem if human intervention measures were not taken.The N quantitative relationships determined in our study might provide a scientific basis for the establishment of effective measures.

Ca^(2+)-Calmodulin is Involved in Betacyanin Accumulation Induced by Dark in C_3 Halophyte Suaeda salsa

The C3 halophyte Suaeda salsa was used to investigate the roles of Ca^2＋, Ca^2＋ channels, and calmodulin （CAM） in betacyanin metabolism. Seeds of S. salsa were cultured in both the dark and light for 3 days. The fresh weight and betacyanin content were much higher in S. salsa seedlings formed in the dark than in seedlings formed in the light. The addition of Ca^2＋ to the half-strength MS nutrient solution promoted betacyanin accumulation in the dark, whereas Ca^2＋ depletion by EGTA suppressed the dark-induced betacyanin accumulation in shoots of S. salsa. The Ca^2＋ channel blocker LaCl3 also inhibited dark-induced betacyanin accumulation. The highest activity of CaM and the maximum betacyanin content decreased by 51% and 45%, respectively, in shoots of S. salsa seedlings treated with the potent CaM antagonist chlorpromazine in the dark. Furthermore, the other CaM antagonist N-（6-aminohexyl）-5-chloro-l-naphthalenesulfonamide （W-7） also inhibited the activity of CaM and dark-dependent betacyanin accumulation, whereas its less active structural analog N-（6-aminohexyl）- 1-naphthalenesulfonamide （W-5） had little effect on the responses to dark of S. salsa seedlings. These results suggest that Ca^2＋, Ca^2＋-regulated ion channels, and CaM play an important role in dark-induced betacyanin accumulation in the shoots of the C3 halophyte S. salsa.

Dynamic Response of Phragmites australis and Suaeda salsa to Climate Change in the Liaohe Delta Wetland

Because of its unique geographical location and ecological function, the Liaohe Delta Wetland is important in maintaining regional ecological balance and security. Monitoring and evaluating changes in the wetland are therefore of great importance. We used medium-and high-resolution satellite data, meteorological station data, and site measurement data to analyze changes in the area and spatial distribution of Phragmites australis and Suaeda salsa in the Liaohe Delta Wetland from 1998 to 2017, as well as their growth response to the climate change. The results showed that during 1998–2017, the areas of both P. australis and S. salsa wetlands alternated through periods of decreasing,increasing, and then decreasing trends. The annual change in the area and spatial distribution range of S. salsa fluctuated more than that of P. australis. The annual variation of normalized difference vegetation index(NDVI) in P. australis wetland showed an upward trend from 1998 to 2017. The area of P. australis cover that was improved, unchanged, and decreased accounted for 81.8%, 12.3%, and 5.9%, respectively, of the total area;evaporation and wind speed were the main meteorological factors affecting the NDVI;and contribution rates of the climate change and human activities to the NDVI were 73.2% and 26.8%, respectively. The area with vegetation cover being mainly S.salsa that was improved, unchanged, and decreased accounted for 63.3%, 18.3%, and 18.4%, respectively, of the total area;and no meteorological factors significantly affected the NDVI of S. salsa in the region. The interaction between vegetation growth and meteorological factors may help to explain the increasing trend in vegetation cover.The improvement in wetland vegetation also led to carbon sequestration and an increase in sequestration capacity.

Trade-off between leaf chlorophyll and betacyanins in Suaeda salsa in the Liaohe estuary wetland in northeast China

Aims Pigment composition is an important functional trait that can be affected by environmental factors.the objective of this study was to investigate the effect of soil salinity on pigment composition in Suaeda salsa by comparing chlorophyll and betacyanin content in the Liaohe estuary wetland,a typical coastal wetland in northeast China.Methods We investigated the plant biomass,percentage of red leaves and pigment content(chlorophyll a,chlorophyll b and betacyanins)in S.salsa in intertidal and supratidal zones of the upper,middle and lower reaches of the Liaohe estuary wetlands.the Na^(+)content of both the soil and plant was also measured.Full analysis of variance and multivariate analysis were used to compare differences in pig-ment content and Na^(+)content between the supratidal and intertidal zones.Important Findings Pigment composition was significantly affected by soil salinity.With increasing soil salinity,the percentage of red leaves was higher in the intertidal zone than in the supratidal zone.In all three reaches,plants had lower chlorophyll a content and higher betacyanin con-tent in the intertidal zone than in the supratidal zone.Compared to chlorophyll a,chlorophyll b was less sensitive to soil salinity.there were no differences in chlorophyll b content between the intertidal and supratidal zones in the upper and lower reaches.Furthermore,pigment composition was associated with both the plant tissue and soil Na^(+)content.Compared to the supratidal zone,the intertidal zone had a higher Na^(+)content in plants.there was a negative rela-tionship between plant chlorophyll content and soil Na^(+)content,but a positive relationship between betacyanin content and soil Na^(+)content.Overall,the results indicated that there might be a trade-off between leaf chlorophyll and betacyanin content in S.salsa to main-tain its growth and survival in high salinity environments.

Growth characteristics of Suaeda salsa under different soil salinity gradients in controlled experiments

Xinjiang is the province with the largest saline-alkali land in China.The growth of halophytes will be stressed when their salt content reaches the threshold.In this study,the pot experiments were conducted and Suaeda salsa,a typical halophyte,was selected as the object to explore the relationship between growth characteristics and salt content under different soil salinity gradients.Salinity gradients were set according to the maximum soil salinity in the current years of Ebinur Lake Wetland National Nature Reserve(ELWNNR).They were classified using the following threshold values:≤2 g/kg(Control Group),10 g/kg,20 g/kg,30 g/kg,and 40 g/kg.The original spectrum,trilateral position and growth characteristics were used as indexes for analysis.With the increase of soil salt content,the red edge shift occurs first,followed by the blue edge shift.The position of yellow edge,green peak and blue edge were not sensitive to the change of soil salinity.This indicates that the red edge is the best indicator for evaluating plant growth.Compared with the Control Group,when the salt content is less than 10 g/kg,the growth status of plant seems not significantly affected.However,plant growth begins to be stressed when the salt content increases to 20 g/kg,which is a turning point for plant health.The increase of soil salt content can inhibit chlorophyll synthesis and plant growth.Plants begin to die when the salt content reaches 40 g/kg.Therefore,40 g/kg could be regarded as the critical point of salt content which inhibits plant growth.The study also shows that the relationship between plant-height change rate and soil salt content is the most significant,indicating that the plant-height change rate is significantly impacted by soil salt content.

石油污染胁迫下碱蓬(Suaeda glauca)和翅碱蓬(Suaeda salsa)萌发生长的响应特征

为了解石油污染对黄河三角洲滨海湿地翅碱蓬和碱蓬萌发生长的影响,通过盆栽控制实验,研究5个浓度梯度(0、2 500、5 000、7 500、10 000 mg/kg)石油污染下2种碱蓬萌发和幼苗生长的响应特征.结果显示:(1)不同浓度石油污染对2种碱蓬种子萌发率、萌发势的影响不显著(P> 0.05),但均显著缩短了2种碱蓬的种子平均萌发时间,提高了种子的萌发指数(翅碱蓬P <0.05;碱蓬P <0.01);(2)在石油浓度为10 000 mg/kg条件下2种碱蓬的发芽势差异极显著(P <0.01),而其他浓度梯度条件下各项萌发指标的种间差异不显著(P> 0.05);(3)石油污染胁迫对2种碱蓬的株高、叶片数和子叶长皆有显著抑制作用,石油浓度梯度越高、植物生长时间越长,这种抑制作用越明显,且石油污染胁迫对翅碱蓬的抑制程度高于碱蓬.本研究表明10 000 mg/kg浓度梯度下的石油污染可促进2种碱蓬种子的萌发,显著抑制2种碱蓬幼苗的生长;结果可为滨海盐碱地油田石油污染土壤的植物修复提供参考依据.(图7表2参47)

Soil characteristics and ecological thresholds of Suaeda salsa wetlands

Suaeda salsa is an annual euhalophyte in estuarine wetlands.Soil properties of wetlands have an important influence on S.salsa growth.Therefore,the soil ecological thresholds is valuable for the restoration of degraded S.salsa wetlands.The objectives of this present study were to analyze the soil physicochemical properties and evaluate the soil ecological thresholds in the typical degraded areas for S.salsa growth.Soil text components became coarser with increased sand contents and less clay contents,as the higher degree of wetland degradation.Meanwhile,the salt contents in different soil depths gradually increased with the increased degree of degradation of wetlands.Evident changes in soil water content,organic matter content,and cations concentrations were not observed,while the concentrations of these factors were higher in the soil layer of 0-10 cm than those in the 20-30 cm.The soil pH in the 0-10 cm soil layer was lower than that in the 20-30 cm.The content of the three available nutrients did not change evidently with the increasing degree of degradation.The optimum thresholds of soil salinity and water content were 7.073-16.613 g/kg and 31.8-63.2%,respectively.

滴灌条件下种植密度对盐地碱蓬生长及移盐量的影响

为了研究滴灌条件下盐地碱蓬的生长和移盐能力对不同种植密度的响应,在新疆喀什地区分别设置D1(10株/m^(2))、D2(20株/m^(2))、D3(30株/m^(2))、D4(40株/m^(2))、D5(50株/m^(2))、D6(60株/m^(2))共6种种植密度进行大田试验,以期通过控制种植密度调控盐地碱蓬株型,达到高产、高效的目的。结果表明:滴灌条件下,种植密度对盐地碱蓬的生长影响显著,随着种植密度增大,株高先增高后降低,蓬径与茎粗逐渐减小;盐地碱蓬生物量随着密度的增大先提高后降低,呈二次曲线趋势,D3处理的生物量最大,较其他处理提高了7.45%~63.85%,同时,D3处理的移盐量最高,达5931.21 kg/hm^(2),较其他处理高12.49%~59.97%;D3与D4处理水分生产效率相近,分别为6.31、6.14 kg/m3;D3处理水分移盐效率最高,达1.58 kg/m^(3),D4处理次之,为1.41 kg/m^(3)。本研究表明可通过控制种植密度调控盐地碱蓬生长指标,优化株型,进而影响盐地碱蓬的产量与移盐能力,滴灌条件下种植盐地碱蓬应采取30~40株/m^(2)的稀植密度。