Deletion of Salmonella pathogenicity islands SPI-1, 2 and 3 induces substantial morphological and metabolic alternation and protective immune potential

The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple deletion mutants of 3 prominent SPIs(SPI-1, 2, and 3), aiming at the impact of deletion on morphology, carbon source metabolism, adhesion and invasion capacity, in vivo colonization, and immune efficacy in chicks. Our examination revealed that the surface of the single deletion mutants(SM6ΔSPI1, ΔSPI2, and ΔSPI3) exhibited a more rugged texture and appeared to be enveloped in a layer of transparent colloid, whereas the morphology of the triple deletion mutant(SM6ΔSPI1&2&3) remained unaltered when compared to the parent strain. The carbon metabolic spectrum of the SPI mutants underwent profound alterations, with a notable and statistically significant modification observed in 30 out of 95 carbon sources, primarily carbohydrates(17 out of 30). Furthermore, the adhesion capacity of the 4 mutants to Caco-2 cells was significantly reduced when compared to that of the parent strain. Moreover,the invasion capacity of mutants SM6ΔSPI1 and SM6ΔSPI1&2&3 exhibited a substantial decrease, while it was enhanced to varying degrees for SM6ΔSPI3 and SM6ΔSPI2. Importantly, none of the 4 mutants induced any clinical symptoms in the chicks. However, they did transiently colonize the spleen and liver. Notably, the SM6ΔSPI1&2&3mutant was rapidly cleared from both the spleen and liver within 8 days post-infection and no notable pathological changes were observed in the organs. Additionally, when challenged, the mutants immunized groups displayed a significant increase in antibody levels and alterations in the CD3+CD4+ and CD3+CD8+ subpopulations, and the levels of IL-4 and IFN-γ cytokines in the SM6ΔSPI1&2&3 immunized chicken serum surpassed those of other groups.In summary, the successful construction of the 4 SPI mutants lays the groundwork for further exploration into the pathogenic(including metabolic) mechanisms of SPIs and the development of safe and effective live attenuated Salmonella vaccines or carriers.

Drivers,Trends,and Patterns of Changing Vegetation-greenness in Nansha Islands,China from 2016 to 2022

Changes in vegetation status generally also represents changes in the ecological health of islands and reefs(IRs).However,studies are limited of drivers and trends of vegetation change of Nansha Islands,China and how they relate to climate change and human activities.To resolve this limitation,we studied changes to the Normalized Difference Vegetation Index(NDVI)vegetation-greenness index for 22 IRs of Nansha Islands during normal and extreme conditions.Trends of vegetation greenness were analyzed using Sen's slope and Mann-Kendall test at two spatial scales(pixel and island),and driving factor analyses were performed by time-lagged partial correlation analyses.These were related to impacts from human activities and climatic factors under normal(temperature,precipitation,radiation,and Normalized Difference Built-up Index(NDBI))and extreme conditions(wind speed and latitude of IRs)from 2016 to 2022.Results showed:1)among the 22 IRs,NDVI increased/decreased significantly in 15/4 IRs,respectively.Huayang Reef had the highest NDVI change-rate(0.48%/mon),and Zhongye Island had the lowest(–0.29%/mon).Local spatial patterns were in one of two forms:dotted-form,and degradation in banded-form.2)Under normal conditions,human activities(characterized by NDBI)had higher impacts on vegetation-greenness than other factors.3)Under extreme conditions,wind speed(R^(2)=0.2337,P<0.05)and latitude(R^(2)=0.2769,P<0.05)provided limited explanation for changes from typhoon events.Our results provide scientific support for the sustainable development of Nansha Islands and the United Nations‘Ocean Decade’initiative.

Macroinvertebrate Community in Streams on the Canary Islands: Gradient Analysis and Stressors

This study describes the gradient analysis of the freshwater macroinvertebrate assemblages in eight streams of Tenerife and La Gomera (Canary Islands) over a 16-year period. During this period, a total of 75 taxa belonging to 34 taxonomic families were found. Endemism has an important presence in the streams on both islands, especially regarding Trichoptera and Coleoptera. The overall status of freshwater macroinvertebrates is rather uncertain as recent data on these communities are scarce and focused on a limited number of sites. Overexploitation of aquifers and the diversion of natural water flows for irrigation have resulted in the drying up of numerous natural streams, inevitably endangering the fauna that inhabits them. A reduction in number and abundance of endemic and sensitive species was observed in the majority of the sampled streams resulting in a lower ecological rating. Therefore, it is proposed that the protection of streams of high conservation value is essential to conserve freshwater macroinvertebrate fauna native to the Canary Islands.

U-Pb zircon geochronology of basaltic pyroclastic rocks from the basement beneath the Xisha Islands in the northwestern South China Sea and its geological significance

As one of the micro-blocks dispersed in the South China Sea(SCS), the basement of the Xisha Islands has rarely been drilled because of the thick overlying Cenozoic sediments, which has led to a confused understanding of the pre-Cenozoic basement of the Xisha Islands. Well CK-1, a kilometer-scale major scientific drill in the Xisha Islands in the northwestern SCS, penetrated thick reefal limestone(0–888.4 m) and the underlying basement rocks(888.4–901.4 m). In this study, we present the zircon U-Pb ages of basement basaltic pyroclastic rocks from Well CK-1 in the Xisha Islands of the northwestern SCS to investigate the basement nature of the Xisha microblock. The basement of Well CK-1 consists of basaltic pyroclastic rocks on the seamount. The zircon grains yielded apparent ages ranging from ca. 2 138.9 Ma to ca. 36 Ma. The old group of zircon grains from Well CK-1 was considered to be inherited zircons. Two Cenozoic zircons gave a weighted mean 206Pb/238U age of(36.3 ± 1.1) Ma,Mean Squared Weighted Deviations(MSWD) = 1.2, which may represent the maximum age of the volcano eruption. The Yanshanian inherited zircons(116.9–105.7 Ma and 146.1–130.2 Ma) from Well CK-1 are consistent with the zircons from Well XK-1, indicating that the basement of Chenhang Island may be similar to that of Well XK-1. We propose that the Xisha micro-block may have developed on a uniform Late Jurassic metamorphic crystalline basement, intruded by Cretaceous granitic magma.

Spatiotemporal Evolution of Urban Heat Islands and Optimization of Spatial Network Construction in the Central Urban Area of Fuzhou City, China

Although accelerated urbanization has led to economic prosperity,it has also resulted in urban heat island effects.Therefore,identifying methods of using limited urban spaces to alleviate heat islands has become an urgent issue.In this study,we assessed the spatiotemporal evolution of urban heat islands within the central urban area of Fuzhou City,China from 2010 to 2019.This assessment was based on a morphological spatial pattern analysis(MSPA)model and an urban thermal environment spatial network constructed us-ing the minimum cumulative resistance(MCR)model.Optimization measures for the spatial network were proposed to provide a theor-etical basis for alleviating urban heat islands.The results show that the heat island area within the study area gradually increased while that of urban cold island area gradually decreased.The core area was the largest of the urban heat island patch landscape elements with a significant impact on other landscape elements,and represented an important factor underlying urban heat island network stability.The thermal environment network revealed a total of 197 thermal environment corridors and 93 heat island sources.These locations were then optimized according to the current land use,which maximized the potential of 1599.83 ha.Optimization based on current land use led to an increase in climate resilience,with effective measures showing reduction in thermal environment spatial network structure and function,contributing to the mitigation of urban heat island.These findings support the use of current land use patterns during urban heat island mitigation measure planning,thus providing an important reference basis for alleviating urban heat island effects.

Hexagon-Islands Density and Size Distribution on Growing Single-Walled Carbon Nanotubes in the Kinetic 5-Vertex Model

A kinetic 5-vertex model is used to investigate hexagon-islands formation on growing single-walled carbon nanotubes (SWCNT). In the model, carbon atoms adsorption and migration processes on the SWCNT edge are considered. These two dynamic processes are assumed to be mutually independent as well as mutually dependent as far as the whole growth of the nanotube is concerned. Key physical parameters of the model are the growth time t, the diffusion length Γ defined as the ratio of the diffusion rate D to the carbon atomic flux F and the SWCNT chiral angle. The kinetic equation that describes the nanotube edge dynamics is solved using kinetic Monte Carlo simulations with the Bortz, Kalos and Lebowitz update algorithm. The behaviors of islands density and size distribution are investigated within the growth parameters’ space. Our study revealed key mechanisms that enable the formation of a new ring of hexagons at the SWCNT edge. The growth occurs either by pre-existing steps propagation or by hexagon-islands growth and coalescence on terraces located between dislocation steps, depending on values of model parameters. This should offer a road map for edge design in nanotubes production. We also found that in appropriate growth conditions, the islands density follows Gaussian and generalized Wigner distributions whereas their size distribution at a given growth time shows a decreasing exponential trend.

Effectiveness of heat-reflective asphalt pavements in mitigating urban heat islands: A systematic literature review

Urban heat island(UHI) effect is a growing concern in numerous cities worldwide, which increases urban temperatures. Conventional asphalt pavements are a major contributor to the issue, causing environmental and health concerns. To tackle this issue, the implementation of cool pavements such as heat-reflective asphalt pavements has been introduced. This systematic literature review(SLR) thoroughly examines prior research to assess the effectiveness of heat-reflective asphalt pavements in reducing UHI effects. This SLR was conducted in accordance with the preferred reporting items for systematic review and meta-analysis(PRISMA) guidelines to enhance data reliability and minimize bias. This review process involved establishing review protocol, formulating review questions, systematically selecting articles through identification, screening, eligibility, quality appraisal, and data abstraction and analysis from various databases such as Scopus, Web of Science(WoS), Wiley, Taylor Francis, and Science Direct. Three primary themes and nine sub-themes were derived from the three review questions. From the results, heat-reflective asphalt pavements effectively minimized the UHI effect. However, their efficiency varies depending on factors such as pavement types, paving location, and use of cool materials. A comprehensive analysis examined heat-reflective pavement's mechanisms, benefits, and drawbacks. This investigation aimed to enhance comprehension and establish a robust basis for future studies in this field.

Fish Assemblage and Abundance Distribution in Nanji Islands Marine Nature Reserve in Relation to Season Change

The fishing survey station located in the warm waters of the East China Sea is one of the abundant fishery resources of waters;with a large number of economic fish breeding grounds and economic juvenile feeding grounds. Several fish species according to this bottom trawl survey results which appear in the annual survey from winter to autumn give us a total of 58 species, which have appeared throughout the year. But the vast majority of species appears only in certain seasons, according to the sea fish survey and based on seasonal migratory species or species mainly small endemic species. The survey of fish is tropical and subtropical warm water species and warm temperate species, and the history of the area has several types of 397 different species. There are many reasons, in addition to recent years, human disturbance and adverse consequences of environmental changes brought about, but also with the relevant sampling methods and sampler time, the survey sampled only trawl based, from Ping yang Ocean and Fisheries Bureau, the waters are still other jobs method net, gill nets, fishing industry, etc. Survey results show that the type of fish in the summer (58 species) > spring (55 species) > winter (51 species) > autumn (42 species). The type of season was among spring to summer rose, summer to autumn decline. The survey found that in the reef area of the Nanji Islands, no one species of fish is the dominant species throughout the year (according to the dominant species, each species is dominant only in one season). From the dominant species turnover accounting for the four seasons, in winter it has 53.72% of the total biomass or annual biomass, spring it has 41.53% of the overall biomass, summer it has 31.85% and autumn it has 38.56% of total biomass. Visible, seasonal succession of dominant species of fish phenomenon is very obvious, especially in the spring and winter, summer transfers dominate species at this stage show the greatest change, but winter, the transfer of this dominant species changes in minimal. Seasonal changes from biomass and the average annual marine fish survey biomass were 4832.25 g/h. From different seasons, the average biomass in winter (10779.88 g/h) > of the average biomass in spring (3624.1 g/hour) > of the mean biomass in autumn (3158 g/h) > the average Biomass in summer (1767 g/h). All of them show significant seasonal variation of the biomass, in winter and spring, the biomass is generally not high, but significantly higher than in the summer and autumn seasons. Each season, around the coast of southern Zhejiang, is less than the biomass and other catches off, which involves investigating sea location, size range, and the survey ship different network related tools. Another change from the area of biomass, some studies found that the survey of four-season fish of waters above the biological capacity of several kilograms only in some stations, namely stations and the highest biomass in summer appeared in stations 3, 7 and 11. In autumn the highest biomass appeared in stations 3, 6, 20 and 23.

Using Circular Economics Strategies to Manage Tourism on Remote Islands:A SMART Approach

Circular economics theory offers destination managers of remote islands a solution-oriented approach to environmental sustainability. This paper examines the practical benefits of this theory that will support the development of a circular economy regarding sustainable island tourism (SIT). The aim of this paper is to inform discussions regarding the feasibility of applying a practice theory that emphasises changes in the behaviours across three segments, visitors, residents, and enterprises using SMART technologies. To achieve this objective, a SMART indicator system (SIS) was used to identify current practices that would support a circular economy (CE). The key innovation of this study is the creation of a proposed framework that may inform best practices for SIT in the future. The findings highlight the potential opportunity of evidenced-based planning to deliver a circular economy strategy (CES) for remote islands in the future. These insights can potentially impact the way that local authorities and destination managers approach the planning of tourism for six islands along the west coast of Ireland.

TIL20: A review of island biogeography and habitat fragmentation studies on subtropical reservoir islands of Thousand Island Lake, China

Reservoir islands formed by dam construction have the same history,clear boundaries,and large numbers that provide a natural platform for testing theories in ecology and biogeography.In this paper,we review studies of multiple zoological taxa on reservoir islands of a large lake in eastern China(Thousand Island Lake).This lake,created in 1959,has 1078 artificial land-bridge islands of varying areas and isolation.Our review summarizes the decades-long studies in island biogeography and habitat fragmentation from this island system,grouped into three topics:species richness(“how many species are there”),community structure(“who are they”),and species interaction(“how they interact with each other”).Our findings support the predictions of the Equilibrium Theory of Island Biogeography and extend this theory to predict community structure of island faunas by moving beyond assumptions of species equivalency.In addition,the extensive studies on ecological networks,including mutualistic,antagonistic,and parasitic interactions,reveal the negative impacts of habitat loss on the maintenance of such networks,even as increasing forest edge enhances the robustness of pollination networks.At the end of this review,we proposed several future research directions based on current studies that are simultaneously at the frontier of ecology and biogeography.

Hydrodynamic Performance of A Floating Breakwater System Under the Terrain of Islands and Reefs: A 3D Experimental Study

With the acceleration of marine construction in China,the exploitation and utilization of resources from islands and reefs are necessary.To prevent and dissipate waves in the process of resource exploitation and utilization,a more effective method is to install floating breakwaters near the terrain of islands and reefs.The terrain around islands and reefs is complex,and waves undergo a series of changes due to the impact of the complex terrain in transmission.It is important to find a suitable location for floating breakwater systems on islands and reefs and investigate how the terrain affects the system’s hydrodynamic performance.This paper introduces a three-cylinder floating breakwater design.The breakwater system consists of 8 units connected by elastic structures and secured by a slack mooring system.To evaluate its effectiveness,a 3D model experiment was conducted in a wave basin.During the experiment,a model resembling the islands and reefs terrain was created on the basis of the water depth map of a specific region in the East China Sea.The transmission coefficients and motion responses of the three-cylinder floating breakwater system were then measured.This was done both in the middle of and behind the islands and reefs terrain.According to the experimental results,the three-cylinder floating breakwater system performs better in terms of hydrodynamics when it is placed behind the terrain of islands and reefs than in the middle of the same terrain.

Field Soil Respiration Rate on a Sub-Antarctic Island: Its Relation to Site Characteristics and Response to Added C, N and P

Botanical, soil chemistry and soil microbiology variables were tested as predictors of in situ soil respiration rate in the various terrestrial habitats on sub-Antarctic Marion Island (47oS, 38oE). Inorganic P and total N concentration were the best predictors amongst the chemistry variables and bacteria plate count the best of the microbiology variables. However, while these chemistry and microbiology variables could accurately predict soil respiration rate for particular habitats, they proved inadequate predictors across the whole range of habitats. The best suite of predictors comprised only botanical variables (relative covers of five plant guilds) and accounted for 94% of the total across-habitat variation in soil respiration rate. Mean field soil respiration rates (2.1 - 15.5 mmol CO2 m-2 h-1) for habitats not influenced by seabirds or seals are similar to rates in comparable Northern Hemisphere tundra habitats. Seabird and seal manuring enhances soil respiration rates to values (up to 27.6 mmol CO2 m-2 h-1) higher than found at any tundra site. Glucose, N, P or N plus P were added to three habitats with contrasting soil types;a fellfield with mineral, nutrient-poor soil, a mire with organic, nutrient-poor soil and a shore-zone herbfield heavily manured by penguins and with organic, nutrient-rich soil. Glucose addition stimulated soil respiration in the fellfield and mire (especially the former) but not in the coastal herbfield soil. N and P, alone or together, did not stimulate respiration at any of the habitats, but adding glucose to fellfield soils that had previously been fortified with P or NP caused a similar increase in respiration rate, which was greater than the increase when adding glucose to soils fortified only with N. This suggests that fellfield soil respiration is limited by P rather than N, and that there is no synergism between the two nutrients. For the mire and coastal herbfield, adding glucose to soils previously fortified with N, P or NP did not enhance rates more than adding glucose to soils that had received no nutrient pre-treatment.

Spatial Variation in Soil Chemistry on a Sub-Antarctic Island

On both west and east sides of sub-Antarctic Marion Island (47oS, 38oE), total Na and exchangeable Na, Mg and K concentrations in the soil decline with increasing distance inland and altitude, related to a decrease in the intensity of seaspray deposition. On the east side, the coastal plain is wide and slopes gently up to the mountainous interior and total C, total N and soil moisture content all decrease significantly, whereas bulk density increases significantly, as one moves away from the sea, reflecting a gradual change from organic, wet, low bulk density peats characteristic of low-land coastal regions to mineral, dry, high bulk density volcanic soils characteristic of inland areas. On the west side, the narrow coastal plain is bounded by an escarpment that rises up very steeply to the highland interior. There, sampling was largely restricted to the coastal plain (soils are rare on the escarpment and interior) and did not cover the same transition from organic to mineral soils as on the east side. Hence, total C, total N and bulk density did not change significantly with increasing distance inland on the west side. Most total Mg is in the mineral fraction of the soil, with a lesser contribution by organic, exchangeable and soil solution forms of Mg. On the east side the gradual transition from highly organic peats to very mineral soils results in an increase in total Mg going inland, but on the west, where there was not this change in soil minerality, total Mg decreased with increasing distance inland, reflecting the decreasing intensity of seaspray. Once the between-side differences in the influence of altitude and distance from the sea are accounted for, there are significant differences in soil chemical composition between the two sides of the island. Overall, west side soils are more influenced by both seaspray and the parent volcanic basalts than are east side soils.

Physical and Mechanical Properties of Coral Sand in the Nansha Islands

Coral sand is a unique material developed in the tropical ocean environment, which is mainly composed of coral and other marine organism debris, with the CaCO3 content up to 96 %. It has special physical and mechanical properties due to its composition, structure and sedimentary environment. In this contribution, we discuss its specific gravity, porosity ratio compressibility, crushing, shearing and intensity for coral sand samples from the Nansha islands based on laboratory mechanical tests. Our results show distinct high porosity ratio, high friction angle and low intensity as compared with the quartz sand. We believe that grain crushing is the main factor that influences the deformation and strength of coral sand. Comprehensive study on the physical and mechanical properties of coral sands is significant in providing reliable scientific parameters to construction on coral islet, and thus avoids accidents in construction.

Numerical modeling of seabed morphological effects from the construction of artificial islands in Danzhou, Hainan

Haihua Islands is a large artificial island in Danzhou, Hainan. The construction of Haihua Islands changes the hYdrodynamic environment of Yangpu waters, and further affects its morphological change. Delft3D is used to set up a two dimensional nested hydrodynamic and sediment model for Yangpu waters in this paper, and this paper focuses on simulating the velocity and morphological change due to the construction of Haihua Islands after the verification of the model. The seabed deposition is small because of low suspended sediment concentration and less sand source near Yangpu waters. The bed level erodes in the south area of Xiaochan Reef and the Yangpu channel due to the velocity increase in the area.

Evaluation of Typhoon Waves Simulated by Wave Watch-Ⅲ Model in Shallow Waters Around Zhoushan Islands

In this study, we simulated typhoon waves in the shallow waters around the Zhoushan Islands using the WaveWatch-Ⅲ(WW3) model version 5.16, the latest version released by the National Oceanic and Atmospheric Administration. Specifically, we used in-situ measurements to evaluate the performance of seven packages of input/dissipation source terms in the WW3 model. We forced the WW3 model by wind fields derived from a combination of the parametric Holland model and high-resolution European Center for Medium-Range Weather Forecasts(ECMWF) wind data in a 0.125? grid, herein called H-E winds. We trained the H-E winds by fitting a shape parameter B to buoy-measured observations, which resulted in a smallest root mean square error(RMSE) of 3 m s^(-1) for B, when treated as a constant 0.4. Then, we applied the seven input/dissipation terms of WW3, labelled ST1, ST2, ST2+STAB2, ST3, ST3+STAB3, ST4, and ST6, to simulate the significant wave height(SWH) up to 5 m during typhoons Fungwong and Chan-hom around the Zhoushan Islands. We then compared the SWHs of the simulated waves with those measured by the in-situ buoys. The results indicate that the simulation using ST2 performs best with an RMSE of 0.79 m for typhoon Fung-wong and an RMSE of 1.12 m for typhoon Chan-hom. Interestingly, we found the simulated SWH results to be relatively higher than those of the observations in the area between Hangzhou Bay and the Zhoushan Islands. This behavior is worthy of further investigation in the future.

Impact factors identification of spatial heterogeneity of herbaceous plant diversity on five southern islands of Miaodao Archipelago in North China

Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity.Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns.Five southern islands in the Miaodao Archipelago,North China were studied herein.The spatial distribution of herbaceous plant diversity on these islands was analyzed,and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA.The results reveal 114 herbaceous plant species,belonging to 94 genera from 34 families in the50 plots sampled.The total species numbers on different islands were significantly positively correlated with island area,and the average a diversity was correlated with human activities,while the(3 diversity among islands was more affected by island area than mutual distances.Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude,slope,total nitrogen,total carbon,and canopy density,and lower moisture content,pH,total phosphorus,total potassium,and aspect.Among the environmental factors,pH,canopy density,total K,total P,moisture content,altitude,and slope had significant gross effects,but only canopy density exhibited a significant net effect.Terrain affected diversity by restricting plantation,plantation in turn influenced soil properties and the two together affected diversity.Therefore,plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands.

Ferroan dolomites in Miocene sediments of the Xisha Islands and their genetic model

Carbonate rocks are important reservoirs for global petroleum exploration.The largest oilfield in the South China Sea,Liuhua 11-1,is distributed in the massive carbonate reef area of the Zhujiang(Pearl)River Mouth Basin.Previous studies showed that one 802.17-m-long core from well Xichen-1 in the South China Sea mainly consisted of white and light gray-white organic reefs.Recently,a Miocene whole core(161.9 m long)of well Xiyong-2,near well Xichen-1,was found to contain six layers of yellowish brown,light yellowish gray,iron black,or light yellowish gray-white organic reefs.Scanning electron microscope images of these layers reveal a typical ferroan dolomite rich in Fe(up to 29%),with the high concentrations of Mn,Cu,W,Zn,Cr,Ni,and Co.Systematic X-ray powder diffraction analysis yields a 1.9–6.1 match in phase ratio with ankerite,5.4–26.9 with dolomite,and zero with calcite,which indicate that the samples can be classified as ferroan dolomite.The iron and heavy metals are inferred to be originated from multiple volcanic eruptions of Gaojianshi Island in the Dongdao Atoll during the middle-late Miocene.These elements were dissolved in seawater,likely as a sol,and carried to Yongxing Island in the Xuande Atoll by sea currents and tides enhanced by prevailing winds,and deposited as a part of the sedimentation process in the study area.The ferroan dolomite has Sr content of (125–285)×10^(-6),which is lower than the accepted Sr boundary value of dolomite.This finding suggests that dolomitization occurred during large-scale global glacial regression in the late Miocene.The isolated Xisha carbonate platform,exposed to air,underwent freshwater leaching and dolomitization induced by mixed water,and caused the extensive Fe-Mg exchange along the organic reef profile to form ankerite and ferroan dolomite.These results may help to understand paleoceanographic environmental changes in the South China Sea during the Miocene.

Survey of reefs based on Landsat 8 operational land imager(OLI)images in the Nansha Islands, South China Sea

A detailed survey of the reefs in the Nansha Islands＇ sea that are of strategic importance is conducive to their future development. In this study, a total of 50 Landsat 8 operational land imager（OLI） images were used to analyze the geological features and spectral characteristics of the Nansha reefs. The exposed islands/sandbanks,reef flats and sub-tidal reefs were identified with the near-infrared band, the red and green bands, and the blue band, respectively. Based on the relationships among various characteristics and bands illustrated in the OLI images, the maximum between-cluster variance threshold algorithm（OTSU method） and the mathematical morphology were employed to extract characteristic parameters of the spatial geometry of the reefs from top to bottom, which were subsequently operated by a series of post-processing methods such as vectorization,simplification and topological analysis. Among the 132 standalone reefs and 16 atolls that are identified in the study, four reefs have yet to be named in the Standard Names of Various Islands in the South China Sea and the website Nansha Islands Online（http：//www.nansha.org/）. Another 24 ones are exposed to the atmosphere even during high tides. Taiping Island with an area of 0.57 km^2 represents the largest exposed geological feature. This study demonstrated the applicability of medium-resolution satellite images to derive the coral reef information effectively and thus provided information for the related departments that are responsible to manage the coast.An improved resolution of multispectral bands with the panchromatic band in higher spatial resolution（15 m for OLI image） is expected to provide an optimum satellite based approach to map marine habitats.

Monitoring Intra-annual Spatiotemporal Changes in Urban Heat Islands in 1449 Cities in China Based on Remote Sensing

This study aimed to accurately study the intra-annual spatiotemporal variation in the surface urban heat island intensities(SUHIIs) in 1449 cities in China.First, China was divided into five environmental regions.Then, the SUHIIs were accurately calculated based on the modified definitions of the city extents and their corresponding nearby rural areas.Finally, we explored the spatiotemporal variation of the mean, maximum, and minimum values, and ranges of SUHIIs from several aspects.The results showed that larger annual mean daytime SUHIIs occurred in hot-humid South China and cold-humid northeastern China, and the smallest occurred in arid and semiarid west China.The seasonal order of the SUHIIs was summer > spring > autumn > winter in all the temperate regions except west China.The SUHIIs were obviously larger during the rainy season than the dry season in the tropical region.Nevertheless, significant differences were not observed between the two seasons within the rainy or dry periods.During the daytime, the maximum SUHIIs mostly occurred in summer in each region, while the minimum occurred in winter.A few cold island phenomena existed during the nighttime.The maximum SUHIIs were generally significantly positively correlated with the minimum SUHIIs during the daytime, nighttime and all-day in all environmental regions throughout the year and the four seasons.Moreover, significant correlation scarcely existed between the daytime and nighttime ranges of the SUHIIs.In addition, the daytime SUHIIs were also insignificantly correlated with the nighttime SUHIIs in half of the cases.