The concentrations of 18 elements in subcellular fractions of human liver were determined by combining differential centrifugation and INAA. Samples of human liver were homogenized in a buffer. The homogenate was sepa...The concentrations of 18 elements in subcellular fractions of human liver were determined by combining differential centrifugation and INAA. Samples of human liver were homogenized in a buffer. The homogenate was separated into nuclei, mitochondrial, lysosomal, microsomal and cytosol fractions by successive differential centrifugation. Biological standard reference materials were used to evaluate the accuracy of the INAA method, and the results agree with the certified values. Element levels in subcellular fractions of human liver were discussed.展开更多
Earthworms have the ability to accumulate of heavy metals, however, there was few studies that addressed the metals in earthworm at subcellular levels in fields. The distributions of metals （Cd, Cu, Zn, and Pb） in s...Earthworms have the ability to accumulate of heavy metals, however, there was few studies that addressed the metals in earthworm at subcellular levels in fields. The distributions of metals （Cd, Cu, Zn, and Pb） in subcellular fractions （cytosol, debris, and granules） of earthworm Metaphire californica were investigated. The relationship between soil metals and earthworms were analyzed to explain its high plasticity to inhabit in situ contaminated soil of Hunan Province, south China. The concentration of Cd in subcellular compartments showed the same pattern as Cu in the order of cytosol 〉 debris 〉 granules. The distribution of Zn and Pb in earthworms indicated a similar propensity for different subcellular fractions that ranked as granules 〉 debris 〉 cytosol for Zn, and granules 〉 cytosol 〉 debris for Pb. The internal metal concentrations in earthworms increased with the soil metals （p 〈 0.05）. Significant positive correlations were found between soil Cd and Cd concentrations in cytosol and debris （p 〈 0.01）. Moreover, the soil Pb concentration significantly influenced the Pb concentrations in cytosol and debris （p 〈 0.01）, similar to that of Cd. The soil Cu concentrations was only associated with the Cu in granules （p 〈 0.05）. Soil Zn concentrations correlated with the Zn concentrations in each subcellular fraction （p 〈 0.05）. Our results provide insights into the variations of metals partitioning in earthworms at subcellular levels and the relationships of soil metals, which could be one of the detoxiflcation strategies to adapt the long-term contaminated environment.展开更多
AIM:To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide(P ODN) which can inhibit cerebral thrombosis.METHODS:(1)Oligodeoxynucleotide was synthesized with phos...AIM:To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide(P ODN) which can inhibit cerebral thrombosis.METHODS:(1)Oligodeoxynucleotide was synthesized with phosphoramidite method in solid phase,3’ end was modified with thiophosphoter.(2)Uptake and subcellular distribution of P ODN.P ODN was given directly.We observed total uptake rate of P ODN by ECV304 cells and subcellular distribution of P ODN.RESULTS:(1)ECV cells uptake more P ODN with increase of concentration,the uptake rate was about 10.8%-11.9%.(2)Subcellular distribution of P ODN:P ODN was mainly distributed in nucleus especially nucleus sediments(69.3%-77.2%)(P< 0.05).CONCLUSION:P ODN suppressing cerebral thrombosis has favorable transmembrane ability,enzyme resistance and stability.展开更多
背景与目的:存活蛋白(survivin)属于凋亡抑制蛋白(inhibitor ofapoptosis of the protein,IAP)家族中一员,已证实表达于多种肿瘤组织中,已有报道认为在不同肿瘤亚细胞中survivin均有表达。本实验初步探索survivin蛋白在非小细胞肺癌(non...背景与目的:存活蛋白(survivin)属于凋亡抑制蛋白(inhibitor ofapoptosis of the protein,IAP)家族中一员,已证实表达于多种肿瘤组织中,已有报道认为在不同肿瘤亚细胞中survivin均有表达。本实验初步探索survivin蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)胞内不同定位所代表的临床意义。方法:运用免疫组化方法检测51例NSCLC组织、21例癌旁组织中survivin的表达,并采用SPSS13.0软件将检测的各项指标与患者各项临床参数进行相关统计分析。结果:Survivin在胞浆和/或胞核均有表达,单纯胞浆阳性率为49.0%(25/51),胞浆阳性率为72.5%(37/51);单纯胞核阳性率为3.9%(2/51),胞核阳性率为27.5%(14/51);胞浆和胞核双阳性约占23.5%(12/51)。肿瘤组阳性率(76.5%,39/51)显著高于癌旁组织(19.0%,4/21)(P=0.000)。Survivin浆阳性状态与分化程度显著相关(P=0.002);核阳性及双阳性状态均与临床分期和N分期明确相关(P<0.05),Ⅲ+Ⅳ期与N1-2期患者阳性率明显高于Ⅰ+Ⅱ期和N0期患者(P<0.05),survivin核阳性组5年生存时间明显缩短(P<0.05)。多因素Cox回归分析提示临床分期和复发转移状态为影响生存的因素。结论:Survivin可能与NSCLC的发生发展相关。Survivin核阳性可能预示着肿瘤的浸润和进展,并可能代表着较差的预后。展开更多
The distribution of selenium\|containing proteins in the subcellular fractions of human liver components was treated by sodium dodecyl sulfate\|polyacrylamide gel electrophoresis (SDS\|PAGE) and hydride generation\|at...The distribution of selenium\|containing proteins in the subcellular fractions of human liver components was treated by sodium dodecyl sulfate\|polyacrylamide gel electrophoresis (SDS\|PAGE) and hydride generation\|atomic fluorescence spectrophotometry (HG\|AFS). About 24 kinds of Se\|containing proteins or protein subunits were found in subcellular fractions. Their molecular weights were mostly ranged from 20 to 30 kD and from 50 to 80 kD. Among them, about 61 kD and 21 kD Se\|containing proteins existed in each fraction with higher concentration. The 54 kD Se\|containing protein existed in nuclei, mitochondria, microsome and cytosol. The specific subcellular distributions of Se\|containing proteins suggest that Se could play specific important biological roles in each organelle.展开更多
文摘The concentrations of 18 elements in subcellular fractions of human liver were determined by combining differential centrifugation and INAA. Samples of human liver were homogenized in a buffer. The homogenate was separated into nuclei, mitochondrial, lysosomal, microsomal and cytosol fractions by successive differential centrifugation. Biological standard reference materials were used to evaluate the accuracy of the INAA method, and the results agree with the certified values. Element levels in subcellular fractions of human liver were discussed.
基金supported by the National Natural Science Foundation of China(No.41471410)the Key Beijing Discipline of Ecology(No.XK10019440)
文摘Earthworms have the ability to accumulate of heavy metals, however, there was few studies that addressed the metals in earthworm at subcellular levels in fields. The distributions of metals （Cd, Cu, Zn, and Pb） in subcellular fractions （cytosol, debris, and granules） of earthworm Metaphire californica were investigated. The relationship between soil metals and earthworms were analyzed to explain its high plasticity to inhabit in situ contaminated soil of Hunan Province, south China. The concentration of Cd in subcellular compartments showed the same pattern as Cu in the order of cytosol 〉 debris 〉 granules. The distribution of Zn and Pb in earthworms indicated a similar propensity for different subcellular fractions that ranked as granules 〉 debris 〉 cytosol for Zn, and granules 〉 cytosol 〉 debris for Pb. The internal metal concentrations in earthworms increased with the soil metals （p 〈 0.05）. Significant positive correlations were found between soil Cd and Cd concentrations in cytosol and debris （p 〈 0.01）. Moreover, the soil Pb concentration significantly influenced the Pb concentrations in cytosol and debris （p 〈 0.01）, similar to that of Cd. The soil Cu concentrations was only associated with the Cu in granules （p 〈 0.05）. Soil Zn concentrations correlated with the Zn concentrations in each subcellular fraction （p 〈 0.05）. Our results provide insights into the variations of metals partitioning in earthworms at subcellular levels and the relationships of soil metals, which could be one of the detoxiflcation strategies to adapt the long-term contaminated environment.
文摘AIM:To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide(P ODN) which can inhibit cerebral thrombosis.METHODS:(1)Oligodeoxynucleotide was synthesized with phosphoramidite method in solid phase,3’ end was modified with thiophosphoter.(2)Uptake and subcellular distribution of P ODN.P ODN was given directly.We observed total uptake rate of P ODN by ECV304 cells and subcellular distribution of P ODN.RESULTS:(1)ECV cells uptake more P ODN with increase of concentration,the uptake rate was about 10.8%-11.9%.(2)Subcellular distribution of P ODN:P ODN was mainly distributed in nucleus especially nucleus sediments(69.3%-77.2%)(P< 0.05).CONCLUSION:P ODN suppressing cerebral thrombosis has favorable transmembrane ability,enzyme resistance and stability.
文摘背景与目的:存活蛋白(survivin)属于凋亡抑制蛋白(inhibitor ofapoptosis of the protein,IAP)家族中一员,已证实表达于多种肿瘤组织中,已有报道认为在不同肿瘤亚细胞中survivin均有表达。本实验初步探索survivin蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)胞内不同定位所代表的临床意义。方法:运用免疫组化方法检测51例NSCLC组织、21例癌旁组织中survivin的表达,并采用SPSS13.0软件将检测的各项指标与患者各项临床参数进行相关统计分析。结果:Survivin在胞浆和/或胞核均有表达,单纯胞浆阳性率为49.0%(25/51),胞浆阳性率为72.5%(37/51);单纯胞核阳性率为3.9%(2/51),胞核阳性率为27.5%(14/51);胞浆和胞核双阳性约占23.5%(12/51)。肿瘤组阳性率(76.5%,39/51)显著高于癌旁组织(19.0%,4/21)(P=0.000)。Survivin浆阳性状态与分化程度显著相关(P=0.002);核阳性及双阳性状态均与临床分期和N分期明确相关(P<0.05),Ⅲ+Ⅳ期与N1-2期患者阳性率明显高于Ⅰ+Ⅱ期和N0期患者(P<0.05),survivin核阳性组5年生存时间明显缩短(P<0.05)。多因素Cox回归分析提示临床分期和复发转移状态为影响生存的因素。结论:Survivin可能与NSCLC的发生发展相关。Survivin核阳性可能预示着肿瘤的浸润和进展,并可能代表着较差的预后。
文摘The distribution of selenium\|containing proteins in the subcellular fractions of human liver components was treated by sodium dodecyl sulfate\|polyacrylamide gel electrophoresis (SDS\|PAGE) and hydride generation\|atomic fluorescence spectrophotometry (HG\|AFS). About 24 kinds of Se\|containing proteins or protein subunits were found in subcellular fractions. Their molecular weights were mostly ranged from 20 to 30 kD and from 50 to 80 kD. Among them, about 61 kD and 21 kD Se\|containing proteins existed in each fraction with higher concentration. The 54 kD Se\|containing protein existed in nuclei, mitochondria, microsome and cytosol. The specific subcellular distributions of Se\|containing proteins suggest that Se could play specific important biological roles in each organelle.
