Developing expressed sequence tag-derived SSR （EST-SSR） markers is imperative in genetic research. In this paper, we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA libr...Developing expressed sequence tag-derived SSR （EST-SSR） markers is imperative in genetic research. In this paper, we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA library. Among the 286 markers designed for the 4 accessions of Glycine max and 6 of its wild progenitor （G. soja） within the subgenus Soja, 209 markers amplified DNA fragments, taking 73.1% and 37 markers appeared to be polymorphic, which was 12.9% of the total. The 37 loci detected a total of 142 alleles, while the PIC values varied from 0.194 to 0.794. Both the number of alleles per locus and PIC value were significantly related to the SSR motif. Six EST-SSR loci may be fixed for different alleles between G. max and G. soja since they were particularly polymorphic among the 6 G. soja accessions. A neighbor-joining tree placed the G. max accessions together as a group within the G. soja, though the average genetic distance among G. soja accessions was much higher. These new EST-SSRs markers will be useful for genetic diversity analysis, genetic mapping construction and gene discovery in Soja subgenus.展开更多
Subgenus Phoenicopsis （Phoenicopsis） assigned to Czekanowskiales is recognized and a new species, Phoenicopsis （Ph.） ordosensis sp. nov. is first reported fi＇om the Middle Jurassic Yan＇an Formation of the Ordos ...Subgenus Phoenicopsis （Phoenicopsis） assigned to Czekanowskiales is recognized and a new species, Phoenicopsis （Ph.） ordosensis sp. nov. is first reported fi＇om the Middle Jurassic Yan＇an Formation of the Ordos Basin, alternate based upon the leaf gross morphology and epidermal structures. The new species has linear leavs in bundles of five to six attached on a short shoot, more than 100 mm long by 5-6 mm wide, containing 10-12 parallel veins at upper portion. The leaf is hypostomatic. Epidermal cells are longitudinally arranged with smooth periclinal wall on the upper cuticle. The narrow non-stomatal zones and wide stomatal zones alternate in the lower cuticle. Stomata in four to five irregular spaced rows are longitudinally oriented in each stomatal zone. Guard cells for each are surrounded by four to five subsidiary cells with undeveloped papillae. Anticlinal wails of ordinal3, epidermal cells are nearly straight and periclinal walls slightly papillate. The new species shallow stomatal pits kept open and surrounded by unevenly undeveloped bulges. These characters are consistent with those of growth in a humid, rather than an arid climate. The presence of subgenus Phoenicopsis and other taxa of Czekanowskiales in the coal-bearing Yan＇an Formation supports the view that the climate in the Ordos Basin was humid and warm with seasonal changes during the Middle Jurassic.展开更多
Five Chinese species of the genus Ectomocoris are revised, keyed and well illustrated by 28 figures. Biarmocoris subgen. n. is established for E. yayeyamae. E. maldonadoi sp. n. is described. E. distanti Cai et Lu is ...Five Chinese species of the genus Ectomocoris are revised, keyed and well illustrated by 28 figures. Biarmocoris subgen. n. is established for E. yayeyamae. E. maldonadoi sp. n. is described. E. distanti Cai et Lu is a new name for E. erebus Distant 1904, junior homonym of E. erebus (Distant, 1903). Lectotypes are designated. E. fiavomaculatus is not represented in China. All the male genitalia are reported for the first time.展开更多
Objective: To determine spatial distribution of sand flies(Diptera: Psychodidae; Larroussius group), the vectors of visceral leishmaniasis in Ardabil province, Northwest of Iran.Methods: Sand flies were collected usin...Objective: To determine spatial distribution of sand flies(Diptera: Psychodidae; Larroussius group), the vectors of visceral leishmaniasis in Ardabil province, Northwest of Iran.Methods: Sand flies were collected using sticky traps from the 30 selected points in Ardabil province, during May-November 2017.The MaxEnt model in GIS software was used for modeling.Results: A total of 2 794 specimens of sand flies were collected, of which 33% were Larroussius subgenus sand flies.Phlebotomus kandelakii and Phlebotomus wenyoni were the highest and lowest collected species respectively.Based on the modeling, four areas in the province were identified with more than 70% probability of the presence of Larroussius group vectors which were at risk of visceral leishmaniasis disease transmission.Conclusions: The distribution of Larroussius subgenus sand flies was observed in all parts of Ardabil.But the northern parts of the province(Germi and Bilesavar counties) as well as central part(Ardabil and Meshkinshahr counties) were of great importance in terms of the presence of Larroussius subgenus sand flies and the possibility of transmission of the visceral leishmaniasis.展开更多
The widespread phalloid fungus Phallus rubicundus is reported for the first time in South Brazil.The collection is described and illustrated macro-and micromorphologically,and the taxonomy,distribution and ecology of ...The widespread phalloid fungus Phallus rubicundus is reported for the first time in South Brazil.The collection is described and illustrated macro-and micromorphologically,and the taxonomy,distribution and ecology of the species are discussed,as well its occurrence in Brazil.展开更多
Floral ontogeny is described in two species of genus Magnolia （Magnollaceae）, Magnolia alboserlcea Chun et C. Tsoong, and M. amoena Cheng, representing subgenus Magnolia and subgenus Yulani In Magnolia, by using sca...Floral ontogeny is described in two species of genus Magnolia （Magnollaceae）, Magnolia alboserlcea Chun et C. Tsoong, and M. amoena Cheng, representing subgenus Magnolia and subgenus Yulani In Magnolia, by using scanning electron microscope （SEM）. The sequence of Initiation of floral organs Is from proximal to distal. The three distinct outermost and middle organs are Initiated in sequence, but ultimately form a single whorl, thus their ontogeny Is consistent with a sepal Interpretation. The last three tepals （petals） alternate with the preceding tepal whorl. The members of androeclum and gynoeclum arise spirally, although the androecium shows some Intermedlacy between a spiral and whorled arrangement. The carpel prlmordia initiate in group of four to five. The order of stamen Initiation within each tier Is not determined. The floral ontogeny Is remarkably homogeneous between the subgenus Magnolia and subgenus Yulani that does not support the resuming of genus Yulani.展开更多
A new species of Culicoides ( Jilinocoides ), C. ( J. ) guangxiensis sp. nov. from Guangxi Zhuang Autonomous Region, China is described. The new species is closely allied to Culicoides qianweiensis Yu 1982,but is dist...A new species of Culicoides ( Jilinocoides ), C. ( J. ) guangxiensis sp. nov. from Guangxi Zhuang Autonomous Region, China is described. The new species is closely allied to Culicoides qianweiensis Yu 1982,but is distinctly different in the presentation of the sensilla coeloconica, sensory pit of the palpus third segment,number of mandible teeth, pale spot on basal portion of wing and r-m pale spot on wing of female. The type spec-imen is deposited in the Institute of Military Medical Sciences, Shenyang Military District, Shenyang 110034,China.展开更多
The dichloromethane extract of the air-dried leaves of Ardisia cf.elliptica(subgenus Tinus)afforded a mixture of bauerenol(1a),α-amyrin(1b)andβ-amyrin(1c).Their structures were identified by NMR spectroscopy.Mixture...The dichloromethane extract of the air-dried leaves of Ardisia cf.elliptica(subgenus Tinus)afforded a mixture of bauerenol(1a),α-amyrin(1b)andβ-amyrin(1c).Their structures were identified by NMR spectroscopy.Mixtures of the triterpenes (1a-1c)at ratios of 2:2:1,2:2:3 and 1:1:1 were tested for their angio-suppressive effects on duck chorioallantoic membrane (CAM).All three ratios were found to be effective in restricting inter-capillary length,while 1a-1c(2:2:1)was most effective in reducing branch point density with 100%CAM viability and embryo survivability,suggesting a high impact angio-suppressive potential of 1a-1c(2:2:1).展开更多
基金supported by the National High-Tech R&D Program of China(863Program,2006AA10A110 and 2006AA10Z164)National Basic Research Program of China(973 Program,2010CB125900 and 2004CB117203)the Academy and Institute Foundation for Basic Scientific Research in Institute of Crop Science,Chinese Academy of Agricultural Sciences
文摘Developing expressed sequence tag-derived SSR （EST-SSR） markers is imperative in genetic research. In this paper, we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA library. Among the 286 markers designed for the 4 accessions of Glycine max and 6 of its wild progenitor （G. soja） within the subgenus Soja, 209 markers amplified DNA fragments, taking 73.1% and 37 markers appeared to be polymorphic, which was 12.9% of the total. The 37 loci detected a total of 142 alleles, while the PIC values varied from 0.194 to 0.794. Both the number of alleles per locus and PIC value were significantly related to the SSR motif. Six EST-SSR loci may be fixed for different alleles between G. max and G. soja since they were particularly polymorphic among the 6 G. soja accessions. A neighbor-joining tree placed the G. max accessions together as a group within the G. soja, though the average genetic distance among G. soja accessions was much higher. These new EST-SSRs markers will be useful for genetic diversity analysis, genetic mapping construction and gene discovery in Soja subgenus.
基金supported by National Natural Science Foundation of China(41172009)Doctoral Fund of Ministry of Education of China(20100061110034)+1 种基金China Geological Survey Grant(1212011120149)Project "111. "of China
文摘Subgenus Phoenicopsis （Phoenicopsis） assigned to Czekanowskiales is recognized and a new species, Phoenicopsis （Ph.） ordosensis sp. nov. is first reported fi＇om the Middle Jurassic Yan＇an Formation of the Ordos Basin, alternate based upon the leaf gross morphology and epidermal structures. The new species has linear leavs in bundles of five to six attached on a short shoot, more than 100 mm long by 5-6 mm wide, containing 10-12 parallel veins at upper portion. The leaf is hypostomatic. Epidermal cells are longitudinally arranged with smooth periclinal wall on the upper cuticle. The narrow non-stomatal zones and wide stomatal zones alternate in the lower cuticle. Stomata in four to five irregular spaced rows are longitudinally oriented in each stomatal zone. Guard cells for each are surrounded by four to five subsidiary cells with undeveloped papillae. Anticlinal wails of ordinal3, epidermal cells are nearly straight and periclinal walls slightly papillate. The new species shallow stomatal pits kept open and surrounded by unevenly undeveloped bulges. These characters are consistent with those of growth in a humid, rather than an arid climate. The presence of subgenus Phoenicopsis and other taxa of Czekanowskiales in the coal-bearing Yan＇an Formation supports the view that the climate in the Ordos Basin was humid and warm with seasonal changes during the Middle Jurassic.
文摘Five Chinese species of the genus Ectomocoris are revised, keyed and well illustrated by 28 figures. Biarmocoris subgen. n. is established for E. yayeyamae. E. maldonadoi sp. n. is described. E. distanti Cai et Lu is a new name for E. erebus Distant 1904, junior homonym of E. erebus (Distant, 1903). Lectotypes are designated. E. fiavomaculatus is not represented in China. All the male genitalia are reported for the first time.
基金funded and supported by the Tehran University of Medical Sciences(Project Number:31437)
文摘Objective: To determine spatial distribution of sand flies(Diptera: Psychodidae; Larroussius group), the vectors of visceral leishmaniasis in Ardabil province, Northwest of Iran.Methods: Sand flies were collected using sticky traps from the 30 selected points in Ardabil province, during May-November 2017.The MaxEnt model in GIS software was used for modeling.Results: A total of 2 794 specimens of sand flies were collected, of which 33% were Larroussius subgenus sand flies.Phlebotomus kandelakii and Phlebotomus wenyoni were the highest and lowest collected species respectively.Based on the modeling, four areas in the province were identified with more than 70% probability of the presence of Larroussius group vectors which were at risk of visceral leishmaniasis disease transmission.Conclusions: The distribution of Larroussius subgenus sand flies was observed in all parts of Ardabil.But the northern parts of the province(Germi and Bilesavar counties) as well as central part(Ardabil and Meshkinshahr counties) were of great importance in terms of the presence of Larroussius subgenus sand flies and the possibility of transmission of the visceral leishmaniasis.
基金the financial support of CNPq(Proc.483455/2013-0)Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná(Conv.675/2014)the curator of the Herbarium SP(Instituto de Botânica,São Paulo)for loan of material.
文摘The widespread phalloid fungus Phallus rubicundus is reported for the first time in South Brazil.The collection is described and illustrated macro-and micromorphologically,and the taxonomy,distribution and ecology of the species are discussed,as well its occurrence in Brazil.
基金Supported by the National Natural Science Foundation of China （30370108 and 30000011） and the Guangdong Provincial Natural Science Foundation of China （5006764）. Acknowledgements The author appreciated Dr Paula Rudall and Mrs Chrissie Prychid in Royal Botanic Gardens, Kew for their supervision and kind help in the laboratory.
文摘Floral ontogeny is described in two species of genus Magnolia （Magnollaceae）, Magnolia alboserlcea Chun et C. Tsoong, and M. amoena Cheng, representing subgenus Magnolia and subgenus Yulani In Magnolia, by using scanning electron microscope （SEM）. The sequence of Initiation of floral organs Is from proximal to distal. The three distinct outermost and middle organs are Initiated in sequence, but ultimately form a single whorl, thus their ontogeny Is consistent with a sepal Interpretation. The last three tepals （petals） alternate with the preceding tepal whorl. The members of androeclum and gynoeclum arise spirally, although the androecium shows some Intermedlacy between a spiral and whorled arrangement. The carpel prlmordia initiate in group of four to five. The order of stamen Initiation within each tier Is not determined. The floral ontogeny Is remarkably homogeneous between the subgenus Magnolia and subgenus Yulani that does not support the resuming of genus Yulani.
文摘A new species of Culicoides ( Jilinocoides ), C. ( J. ) guangxiensis sp. nov. from Guangxi Zhuang Autonomous Region, China is described. The new species is closely allied to Culicoides qianweiensis Yu 1982,but is distinctly different in the presentation of the sensilla coeloconica, sensory pit of the palpus third segment,number of mandible teeth, pale spot on basal portion of wing and r-m pale spot on wing of female. The type spec-imen is deposited in the Institute of Military Medical Sciences, Shenyang Military District, Shenyang 110034,China.
基金supported by the grant from the Philippine Council for Health Research and Development (PCHRD) of the Philippines through the Metro Manila Health Research and Development Consortium (MMHRDC)
文摘The dichloromethane extract of the air-dried leaves of Ardisia cf.elliptica(subgenus Tinus)afforded a mixture of bauerenol(1a),α-amyrin(1b)andβ-amyrin(1c).Their structures were identified by NMR spectroscopy.Mixtures of the triterpenes (1a-1c)at ratios of 2:2:1,2:2:3 and 1:1:1 were tested for their angio-suppressive effects on duck chorioallantoic membrane (CAM).All three ratios were found to be effective in restricting inter-capillary length,while 1a-1c(2:2:1)was most effective in reducing branch point density with 100%CAM viability and embryo survivability,suggesting a high impact angio-suppressive potential of 1a-1c(2:2:1).
