Using the modified HABM (Human amniotic basement membrane) model,an invasive subline(HR-8348-Ⅰ)was selected frorn the human rectal adenocarcinoma cell line (HR-8348).The invasiveness,motility and other biological cha...Using the modified HABM (Human amniotic basement membrane) model,an invasive subline(HR-8348-Ⅰ)was selected frorn the human rectal adenocarcinoma cell line (HR-8348).The invasiveness,motility and other biological characteristics of the selected cells and the parent cells were assayed comparatively. Although no significant differences(P>0. 05) were found in the mitotic index,growth curve,doubling time and plate efficiency,the invasiveness,migrating ability and attachment of the subline cells increased significantly(P<0.01).The subline was nearly triploid,with wider chromosome distribution,and was characterized by possessing more surface projections (filiform pseudopods),thicker ruthenium red-positive coat,than the parent cells , which were hypertriploid.The results suggest that HR-8348-Ⅰis a highly invasive variant isolated from the subpopulation of HR-8348,and could be useful in defining the biochemical and genetic events mediating invasion.展开更多
By using cell cloning technique, 4 sublines (A,C,D,E) were isolated from a cell line of human lung giant cell carcinoma (PLA-801). After subcutaneous inoculation in T-cell deficient BALB/c nude mice, the incidence of ...By using cell cloning technique, 4 sublines (A,C,D,E) were isolated from a cell line of human lung giant cell carcinoma (PLA-801). After subcutaneous inoculation in T-cell deficient BALB/c nude mice, the incidence of tumor growth and spontaneous metastasis were the highest in subline D, moderate in sublines A and E, and lowest in subline C. Tumor cells of subline C also showed similar low tumorigenicity in another T-cell deficient 615/ PB1 nude mice.However, in 615/PB1 beige nude mice with con-genitally combined immune-deficiency in both T and NK cell activity, tumor cells of the rarely metastatic subline C do produce significantly high frequency of tumor growth and spontaneous metastasis.Morphological studies (light microscope, electron microscope and immunohistochemistry) showed rich microfilaments and Vimentin positive in the cytoplasm of metastatic tumor cells. This may imply a possibility that tumor cells differentiate towards the direction favourable to spreading and metastasis.展开更多
The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more th...The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more than 30% within 5 years. 1 Recent studies about osteosarcoma metastatic mechanism are based on osteosarcoma matrilineal cell lines. 2 For further studies of metastatic mechanism of osteosarcoma the establishment of a better metastatic experimental model of osteosarcoma is needed. We isolated and established two cell sublines, with high and low metastatic potentials, respectively, derived from human osteosarcoma MG-63 cell line by cloning in vitro and transplantation in vivo , then analysed and identified their biological characteristics.展开更多
Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at...Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte(Gr cells)and SYSU-OHe-C Plasmatocyte(PI cells),which possess the morphological characteristics of granulocytes and plasmatocytes,respectively,were established.Gr and PI cells showed different behaviors in immune reactions,such as spreading,phagocytosis and encapsulation.PI cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign objects.These results also suggested that granulocytes and plasmatocytes may express some different proteins.By comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmembrane proteins highly ex pressed in PI cells(six)or Gr cells(seven)were further screened and confirmed by reverse-transcription polymerase chain reaction(PCR).Finally,three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis.These genes may potentially be used as molecular markers to distinguish between granulocy tes and plasmatocytes in Ostrinia fiurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.展开更多
文摘Using the modified HABM (Human amniotic basement membrane) model,an invasive subline(HR-8348-Ⅰ)was selected frorn the human rectal adenocarcinoma cell line (HR-8348).The invasiveness,motility and other biological characteristics of the selected cells and the parent cells were assayed comparatively. Although no significant differences(P>0. 05) were found in the mitotic index,growth curve,doubling time and plate efficiency,the invasiveness,migrating ability and attachment of the subline cells increased significantly(P<0.01).The subline was nearly triploid,with wider chromosome distribution,and was characterized by possessing more surface projections (filiform pseudopods),thicker ruthenium red-positive coat,than the parent cells , which were hypertriploid.The results suggest that HR-8348-Ⅰis a highly invasive variant isolated from the subpopulation of HR-8348,and could be useful in defining the biochemical and genetic events mediating invasion.
文摘By using cell cloning technique, 4 sublines (A,C,D,E) were isolated from a cell line of human lung giant cell carcinoma (PLA-801). After subcutaneous inoculation in T-cell deficient BALB/c nude mice, the incidence of tumor growth and spontaneous metastasis were the highest in subline D, moderate in sublines A and E, and lowest in subline C. Tumor cells of subline C also showed similar low tumorigenicity in another T-cell deficient 615/ PB1 nude mice.However, in 615/PB1 beige nude mice with con-genitally combined immune-deficiency in both T and NK cell activity, tumor cells of the rarely metastatic subline C do produce significantly high frequency of tumor growth and spontaneous metastasis.Morphological studies (light microscope, electron microscope and immunohistochemistry) showed rich microfilaments and Vimentin positive in the cytoplasm of metastatic tumor cells. This may imply a possibility that tumor cells differentiate towards the direction favourable to spreading and metastasis.
基金Theprojectwassupportedbythe973ProjectofChina (No 2002CB513107)
文摘The most frequent cause of death of patients with osteosarcoma is the metastasis of tumour cells. In spite of successful control of the primary tumour, the mortality of the patients due to metastatic spread is more than 30% within 5 years. 1 Recent studies about osteosarcoma metastatic mechanism are based on osteosarcoma matrilineal cell lines. 2 For further studies of metastatic mechanism of osteosarcoma the establishment of a better metastatic experimental model of osteosarcoma is needed. We isolated and established two cell sublines, with high and low metastatic potentials, respectively, derived from human osteosarcoma MG-63 cell line by cloning in vitro and transplantation in vivo , then analysed and identified their biological characteristics.
基金the National Key R&D Program of China(2017YFD200400)Guangdong Basic and Applied Basic Research Foundation(2020A 1515010455)+2 种基金the National Natural Science Foundation of China(31272091)the Key Project of Chinese National Programs for Fundamental Research and Development(973 programs,2013CB 127603)the International Foundation for Scicncc(C3680-2).
文摘Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte(Gr cells)and SYSU-OHe-C Plasmatocyte(PI cells),which possess the morphological characteristics of granulocytes and plasmatocytes,respectively,were established.Gr and PI cells showed different behaviors in immune reactions,such as spreading,phagocytosis and encapsulation.PI cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign objects.These results also suggested that granulocytes and plasmatocytes may express some different proteins.By comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmembrane proteins highly ex pressed in PI cells(six)or Gr cells(seven)were further screened and confirmed by reverse-transcription polymerase chain reaction(PCR).Finally,three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis.These genes may potentially be used as molecular markers to distinguish between granulocy tes and plasmatocytes in Ostrinia fiurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.
