This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE) was microinjected into the CNTS of ...This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE) was microinjected into the CNTS of rabbits and mean arterial blood pressure (MAP) and renal nerve discharges (FRND) were synchronously recorded. The results indicated that (1) microinjection of norepinephine into the CNTS of rabbit could significantly attenuate the frequency of renal nerve discharge, and at the same time decrease markedly the mean arterial pressure. (2) Microinjection of 0.3 nmol yohimbin into CNTS had no significant influence on FRND and MAP, but could attenuate and even reverse the effects of NE on FRND and MAP. These results suggest that microinjection of NE into CNTS may activate the alpha-adrenorecptor located in CNTS and secondarily produce a depressor effect by attenuating the activity of peripheral sympathetic nervous system.展开更多
Objective To establish a 3D atlas of the lenticular nuclei and its subnucleus with the cryosection images of the male from "Atlas of Chinese Visible Human". Methods The lenticular nuclei and its subnucleus w...Objective To establish a 3D atlas of the lenticular nuclei and its subnucleus with the cryosection images of the male from "Atlas of Chinese Visible Human". Methods The lenticular nuclei and its subnucleus were segmented from the cryosection images and reconstructed with the software展开更多
Extensive studies have focused on the development and regionalization of neurons in the central nervous system(CNS). Many genes, which play crucial roles in the development of CNS neurons, have been identified. By usi...Extensive studies have focused on the development and regionalization of neurons in the central nervous system(CNS). Many genes, which play crucial roles in the development of CNS neurons, have been identified. By using the technique "direct reprogramming", neurons can be produced from multiple cell sources such as fibroblasts. However, understanding the region-specific regulation of neurons in the CNS is still one of the biggest challenges in the research field of neuroscience. Neurons located in the trigeminal subnucleus caudalis(Vc) and in the spinal dorsal horn(SDH) play crucial roles in pain and sensorimotor functions in the orofacial and other somatic body regions, respectively. Anatomically, Vc represents the most caudal component of the trigeminal system, and is contiguous with SDH. This review is focused on recent data dealing with the regional specificity involved in the development of neurons in Vc and SDH.展开更多
Objective: To discuss mechanism of moxibustion(thermal stimulation) effect and best moxibustion stimulus parameter.Methods: Experiments were performed on 48 male Sprague-Dawley rats. Unit discharges from individual si...Objective: To discuss mechanism of moxibustion(thermal stimulation) effect and best moxibustion stimulus parameter.Methods: Experiments were performed on 48 male Sprague-Dawley rats. Unit discharges from individual single neuron were recorded extracellularly with glass-microelectrode in Subnucleus Reticularis Dorsalis(SRD). Visceral-intrusive stimulation is done by colorectal distension. Thermal stimulation with different temperature(40°C, 42°C, 44°C, 46°C, 48°C, 50°C, 52°C) and different stimulus area(diameter of circle : 1.0 cm, 1.5 cm, 2.0 cm, 2.5 cm, 3.0 cm, 3.5 cm, 4.0 cm) was applied around RN12 during nociceptive colorectal distension.Results: SRD neurons could be activated by visceral stimulation within noxious range. Under low temperature of stimulus, especially under45°C of pain threshold to ordinary people, visceral nociceptive afferent facilitated thermal stimulus from the body surface. While after thermal stimulation reached a harmful degree, the thermal stimulus will inhibit visceral nociceptive afferent. Moreover, statistics show that the higher the temperature is, the smaller the size of stimulation area is needed, and they correlate with each other negatively.Conclusion: Visceral nociception could be inhibited by somatic thermal stimulation with specific parameter at medulla level. According to our finding, best thermal stimulation temperature is around 48°C and the best size of stimulation area is around 3.14-7.07 cm2(with 2.0-3.0 cm diameter).展开更多
文摘This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE) was microinjected into the CNTS of rabbits and mean arterial blood pressure (MAP) and renal nerve discharges (FRND) were synchronously recorded. The results indicated that (1) microinjection of norepinephine into the CNTS of rabbit could significantly attenuate the frequency of renal nerve discharge, and at the same time decrease markedly the mean arterial pressure. (2) Microinjection of 0.3 nmol yohimbin into CNTS had no significant influence on FRND and MAP, but could attenuate and even reverse the effects of NE on FRND and MAP. These results suggest that microinjection of NE into CNTS may activate the alpha-adrenorecptor located in CNTS and secondarily produce a depressor effect by attenuating the activity of peripheral sympathetic nervous system.
文摘Objective To establish a 3D atlas of the lenticular nuclei and its subnucleus with the cryosection images of the male from "Atlas of Chinese Visible Human". Methods The lenticular nuclei and its subnucleus were segmented from the cryosection images and reconstructed with the software
文摘Extensive studies have focused on the development and regionalization of neurons in the central nervous system(CNS). Many genes, which play crucial roles in the development of CNS neurons, have been identified. By using the technique "direct reprogramming", neurons can be produced from multiple cell sources such as fibroblasts. However, understanding the region-specific regulation of neurons in the CNS is still one of the biggest challenges in the research field of neuroscience. Neurons located in the trigeminal subnucleus caudalis(Vc) and in the spinal dorsal horn(SDH) play crucial roles in pain and sensorimotor functions in the orofacial and other somatic body regions, respectively. Anatomically, Vc represents the most caudal component of the trigeminal system, and is contiguous with SDH. This review is focused on recent data dealing with the regional specificity involved in the development of neurons in Vc and SDH.
基金supported by National Basic Research Program of China(973 Program,no.2011CB505201,2009CB522902)National Natural Science Foundation of China(no.81102649)
文摘Objective: To discuss mechanism of moxibustion(thermal stimulation) effect and best moxibustion stimulus parameter.Methods: Experiments were performed on 48 male Sprague-Dawley rats. Unit discharges from individual single neuron were recorded extracellularly with glass-microelectrode in Subnucleus Reticularis Dorsalis(SRD). Visceral-intrusive stimulation is done by colorectal distension. Thermal stimulation with different temperature(40°C, 42°C, 44°C, 46°C, 48°C, 50°C, 52°C) and different stimulus area(diameter of circle : 1.0 cm, 1.5 cm, 2.0 cm, 2.5 cm, 3.0 cm, 3.5 cm, 4.0 cm) was applied around RN12 during nociceptive colorectal distension.Results: SRD neurons could be activated by visceral stimulation within noxious range. Under low temperature of stimulus, especially under45°C of pain threshold to ordinary people, visceral nociceptive afferent facilitated thermal stimulus from the body surface. While after thermal stimulation reached a harmful degree, the thermal stimulus will inhibit visceral nociceptive afferent. Moreover, statistics show that the higher the temperature is, the smaller the size of stimulation area is needed, and they correlate with each other negatively.Conclusion: Visceral nociception could be inhibited by somatic thermal stimulation with specific parameter at medulla level. According to our finding, best thermal stimulation temperature is around 48°C and the best size of stimulation area is around 3.14-7.07 cm2(with 2.0-3.0 cm diameter).
