OBJECTIVE:To reveal the effects of Guasha(scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide(CGRP) and substance P(SP).METHODS:50 rats,as experimental s...OBJECTIVE:To reveal the effects of Guasha(scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide(CGRP) and substance P(SP).METHODS:50 rats,as experimental subjects,were randomly divided into 5 groups according to different observation time points.Dorsal setae were shaved for the exposure of skin on both sides of the spine.Even reinforcing and reducing method was applied to the rats in Guasha groups on the site equivalent to bladder meridian of human body on one side of the spine,and the skin was scraped from top to bottom until rash of measles occurred.The skin tissues with rash of measles were taken down after perfusion.The corresponding sites of rats in group A were also taken down as control.The tissues were made to sections and used for immunofluorescence histochemical staining and HE staining of antibodies such as SP and CGRP.RESULTS:After Guasha,there were significant differences in appearance,hair follicle and blood vessel on local skin scraped on the back of rats when compared with control;In different time points,the differences reduced.There was no significant difference in expression of CGRP and SP when compared local skin scraped on the back of rats in different time points.CONCLUSION:Guasha didn't significantly change the morphology of nerve fibers inside local skin,and the histomorphology of hair follicle,blood vessel and etc.However,after Guasha they basically returned to normal level within five days.展开更多
In temporomandibular disorders（TMD）, pain takes place when neuropeptides stimulate synovial tissue to produce several cytokines such as interleukin（IL）-1β, IL-6 and tumor necrosis factor（TNF）-α, which activate...In temporomandibular disorders（TMD）, pain takes place when neuropeptides stimulate synovial tissue to produce several cytokines such as interleukin（IL）-1β, IL-6 and tumor necrosis factor（TNF）-α, which activate neurons and glia of synovial membrane at the bilaminar regions of temporomandibular joint（TMJ）. It has been reported that, after neurogenic differentiation, the synovial mesenchymal stem cells（SMSCs）, deriving from TMJ, possess the same cytological features as the neuronal cells. This study examined the ability of substance P（SP） and calcitonin gene-related peptide（CGRP） to stimulate SMSCs and neurogenic SMSCs secreting inflammatory cytokines during TMD, evaluated the mutual effects of inflammatory cytokines and neuropeptides and tested the analgesic effect of hyaluronic acid（HA）. The levels of IL-1β, IL-6 and TNF-α in SMSCs and neurogenic SMSCs in the presence of neuropeptides were measured by ELISA. SP and CGRP produced by SMSCs and neurogenic SMSCs were determined by RT-PCR and Western blotting. The results showed that the expression of SP and CGRP was significantly enhanced in the neurogenic SMSCs in response to IL-1β, IL-6 and TNF-α, and the effect was remarkably inhibited by HA. IL-1β, IL-6 and TNF-α, in return, could be enhanced in the neurogenic SMSCs upon stimulation by SP and CGRP. Neuropeptides and inflammatory cytokines might work mutually on the TMD pain. The HA-mediated analgesic effect may be implicated in the inhibition of SP and CGRP expression in neurogenic SMSCs.展开更多
目的镜像神经元疗法对中风后复杂性区域疼痛综合征(CRPS)Ⅰ期的临床疗效及作用机制的探讨。方法选择康复科收治的40例CRPSⅠ期患者为研究对象。在研究中采用随机法,将选择的40例患者分为对照组与镜像组,每组20例。均采取基础以及常规康...目的镜像神经元疗法对中风后复杂性区域疼痛综合征(CRPS)Ⅰ期的临床疗效及作用机制的探讨。方法选择康复科收治的40例CRPSⅠ期患者为研究对象。在研究中采用随机法,将选择的40例患者分为对照组与镜像组,每组20例。均采取基础以及常规康复治疗,镜像组额外追加镜像治疗,两组每天上下午各治疗1次,5次/周,共治疗3周。采用休息状态视觉模拟评分(resting state visual analogue scale,R-VAS)与被动运动视觉模拟评分(passive movement state visual analogue scale,P-VAS)来评估上肢肩关节疼痛状况,选择运用排水法来评估手部肿胀状况,并且选择运用Fugl-Meyer量表,评估上肢运动功能,采用ELISA检测技术进行测定血清降钙素基因相关肽(CGRP)、P物质(SP)含量。结果镜像组患者治疗3周后的肩关节R-VAS、P-VAS评分及肿胀程度均低于对照组(P<0.05),Fugl-Meyer评分高于对照组(P<0.05),SP含量低于对照组(P<0.05),CGRP含量高于对照组(P<0.05)。结论镜像组能更有效地改善CRPSⅠ期患者肩关节疼痛、手部肿胀及上肢功能障碍,调节血清CGRP及SP细胞水平。展开更多
基金the National Natural Science Foundation of China(General Program)(No.81173346No.30672720No.81674079)
文摘OBJECTIVE:To reveal the effects of Guasha(scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide(CGRP) and substance P(SP).METHODS:50 rats,as experimental subjects,were randomly divided into 5 groups according to different observation time points.Dorsal setae were shaved for the exposure of skin on both sides of the spine.Even reinforcing and reducing method was applied to the rats in Guasha groups on the site equivalent to bladder meridian of human body on one side of the spine,and the skin was scraped from top to bottom until rash of measles occurred.The skin tissues with rash of measles were taken down after perfusion.The corresponding sites of rats in group A were also taken down as control.The tissues were made to sections and used for immunofluorescence histochemical staining and HE staining of antibodies such as SP and CGRP.RESULTS:After Guasha,there were significant differences in appearance,hair follicle and blood vessel on local skin scraped on the back of rats when compared with control;In different time points,the differences reduced.There was no significant difference in expression of CGRP and SP when compared local skin scraped on the back of rats in different time points.CONCLUSION:Guasha didn't significantly change the morphology of nerve fibers inside local skin,and the histomorphology of hair follicle,blood vessel and etc.However,after Guasha they basically returned to normal level within five days.
基金supported by the National Natural Science Foundation of China(No.81071266)
文摘In temporomandibular disorders（TMD）, pain takes place when neuropeptides stimulate synovial tissue to produce several cytokines such as interleukin（IL）-1β, IL-6 and tumor necrosis factor（TNF）-α, which activate neurons and glia of synovial membrane at the bilaminar regions of temporomandibular joint（TMJ）. It has been reported that, after neurogenic differentiation, the synovial mesenchymal stem cells（SMSCs）, deriving from TMJ, possess the same cytological features as the neuronal cells. This study examined the ability of substance P（SP） and calcitonin gene-related peptide（CGRP） to stimulate SMSCs and neurogenic SMSCs secreting inflammatory cytokines during TMD, evaluated the mutual effects of inflammatory cytokines and neuropeptides and tested the analgesic effect of hyaluronic acid（HA）. The levels of IL-1β, IL-6 and TNF-α in SMSCs and neurogenic SMSCs in the presence of neuropeptides were measured by ELISA. SP and CGRP produced by SMSCs and neurogenic SMSCs were determined by RT-PCR and Western blotting. The results showed that the expression of SP and CGRP was significantly enhanced in the neurogenic SMSCs in response to IL-1β, IL-6 and TNF-α, and the effect was remarkably inhibited by HA. IL-1β, IL-6 and TNF-α, in return, could be enhanced in the neurogenic SMSCs upon stimulation by SP and CGRP. Neuropeptides and inflammatory cytokines might work mutually on the TMD pain. The HA-mediated analgesic effect may be implicated in the inhibition of SP and CGRP expression in neurogenic SMSCs.
文摘目的镜像神经元疗法对中风后复杂性区域疼痛综合征(CRPS)Ⅰ期的临床疗效及作用机制的探讨。方法选择康复科收治的40例CRPSⅠ期患者为研究对象。在研究中采用随机法,将选择的40例患者分为对照组与镜像组,每组20例。均采取基础以及常规康复治疗,镜像组额外追加镜像治疗,两组每天上下午各治疗1次,5次/周,共治疗3周。采用休息状态视觉模拟评分(resting state visual analogue scale,R-VAS)与被动运动视觉模拟评分(passive movement state visual analogue scale,P-VAS)来评估上肢肩关节疼痛状况,选择运用排水法来评估手部肿胀状况,并且选择运用Fugl-Meyer量表,评估上肢运动功能,采用ELISA检测技术进行测定血清降钙素基因相关肽(CGRP)、P物质(SP)含量。结果镜像组患者治疗3周后的肩关节R-VAS、P-VAS评分及肿胀程度均低于对照组(P<0.05),Fugl-Meyer评分高于对照组(P<0.05),SP含量低于对照组(P<0.05),CGRP含量高于对照组(P<0.05)。结论镜像组能更有效地改善CRPSⅠ期患者肩关节疼痛、手部肿胀及上肢功能障碍,调节血清CGRP及SP细胞水平。
