[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through op...[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through optimizing the medium of Brevibacil us laterosporus. [Method] A total of 10 factors were tested for their function of improving antibiotics yield of BL-21 by single-factor test. And the indicative bacterium was screened. [Result] The best indicative bacterium was Al-ternaria solani, the optimum process parameters in shaking flask on fermentation consisted of initial pH of 8.0, culture at 30 ℃, loadage 90 ml/250 ml, rotation 180 r/min, and fermentation time of 48 h. The results also indicated that glucose was the best carbon source for the antibiotics productivity of BL-21, and beef extract was the best nitrogen source. In addition, univalent ion Cl＋ and divalent ion Mn2＋ stimu-lated BL-21 antibacterial active component biosynthesis. [Conclusion] Optimized medi-um is more conducive to produce antimicrobial substances.展开更多
The Wister fat-storing cells were isolated by perfusion with collagenase and centrifugation with metrizamide density cushion technique and cultured in vitro. The fatstoring cells were confirmed by the presentation of ...The Wister fat-storing cells were isolated by perfusion with collagenase and centrifugation with metrizamide density cushion technique and cultured in vitro. The fatstoring cells were confirmed by the presentation of the lipid drop in the cytoplasm and the visualization of dismin with anti-dismin antibody by using indirect immunofluorescence method. By the videotape recorder (VIR) and the imagine analysis system, we observed the wandering immigration, the abrupt contraction of fat-storing cells with spike, then becoming a ball-like shape in its division phase. After that the cell began to extend and the contraction of these cells can be induced by the presence of 10-2 mmol/L endothelin-1 , 1 mmol/L of substance P and 2×10-5 mmol/L noradrenalin. After removal of these agents the contracted cells would become extended. All these findings indicate that the fat-storing cells have ability of contraction and movement.展开更多
基金Supported by National Natural Science Foundation of China(31370511)Natural Science Foundation of Heilongjiang Province(C201203)Open Fund of Key Laboratory of Microbiology of Heilongjiang University(2012MOI-1)~~
文摘[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through optimizing the medium of Brevibacil us laterosporus. [Method] A total of 10 factors were tested for their function of improving antibiotics yield of BL-21 by single-factor test. And the indicative bacterium was screened. [Result] The best indicative bacterium was Al-ternaria solani, the optimum process parameters in shaking flask on fermentation consisted of initial pH of 8.0, culture at 30 ℃, loadage 90 ml/250 ml, rotation 180 r/min, and fermentation time of 48 h. The results also indicated that glucose was the best carbon source for the antibiotics productivity of BL-21, and beef extract was the best nitrogen source. In addition, univalent ion Cl＋ and divalent ion Mn2＋ stimu-lated BL-21 antibacterial active component biosynthesis. [Conclusion] Optimized medi-um is more conducive to produce antimicrobial substances.
文摘The Wister fat-storing cells were isolated by perfusion with collagenase and centrifugation with metrizamide density cushion technique and cultured in vitro. The fatstoring cells were confirmed by the presentation of the lipid drop in the cytoplasm and the visualization of dismin with anti-dismin antibody by using indirect immunofluorescence method. By the videotape recorder (VIR) and the imagine analysis system, we observed the wandering immigration, the abrupt contraction of fat-storing cells with spike, then becoming a ball-like shape in its division phase. After that the cell began to extend and the contraction of these cells can be induced by the presence of 10-2 mmol/L endothelin-1 , 1 mmol/L of substance P and 2×10-5 mmol/L noradrenalin. After removal of these agents the contracted cells would become extended. All these findings indicate that the fat-storing cells have ability of contraction and movement.
