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小麦蔗糖非发酵相关蛋白激酶SnRK3基因的克隆与分析
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作者 杨艳 黄锦雯 +3 位作者 夏鹏亮 李义婷 吴兴阳 马东方 《植物保护学报》 CAS CSCD 北大核心 2023年第1期161-169,共9页
为明确小麦蔗糖非发酵-1相关蛋白激酶(sucrose non-ferment-1-related protein kinase,SnRK)在协调植物体内多种信号通路之间的作用,通过普通PCR方法从扬麦20中克隆SnRK基因,利用生物信息学方法对其进行分析,使用实时荧光定量PCR技术分... 为明确小麦蔗糖非发酵-1相关蛋白激酶(sucrose non-ferment-1-related protein kinase,SnRK)在协调植物体内多种信号通路之间的作用,通过普通PCR方法从扬麦20中克隆SnRK基因,利用生物信息学方法对其进行分析,使用实时荧光定量PCR技术分析其在激素、干旱、盐和病菌胁迫下的表达模式,并测定瞬时过表达该基因对烟草叶片抗致病疫霉Phytophthora infestans侵染能力的影响。结果显示,从扬麦20中克隆获得SnRK3基因,命名为TaSnRK3.16-D;该基因编码蛋白可能通过与多种蛋白互作参与多种形式的调控;过表达TaSnRK3.16-D增强了小麦对脱落酸、NaCl、PEG、白粉病菌Blumeria graminis f.sp.tritici和赤霉病菌Fusarium graminearum胁迫的抗性,经亚细胞定位和根癌农杆菌Agrobacterium tumefaciens瞬时表达试验验证表明TaSnRK3.16-D定位于细胞膜上,且其在烟草叶片上过表达部位的致病疫霉侵染病斑颜色较对照病斑颜色浅,一定程度上抑制了致病疫霉的侵染。表明SnRK基因在小麦抗逆过程中发挥了一定作用。 展开更多
关键词 生物信息学分析 蔗糖非发酵-1相关蛋白激酶(snrk) 生物胁迫 非生物胁迫 小麦
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植物SnRK家族的研究进展 被引量:7
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作者 孔伟胜 刘言 +2 位作者 王林娟 李胜飞 张海荣 《植物生理学报》 CAS CSCD 北大核心 2016年第4期413-422,共10页
植物在自然界中面临各种环境侵害时候,如干旱、盐、低温和病菌袭击,会启动自身的抵御机制来适应各种侵害。蔗糖非发酵相关的蛋白激酶(sucrose non-fermenting-1-related protein kinase,SnRK)是广泛存在于植物中的一类Ser/Thr蛋白激酶,... 植物在自然界中面临各种环境侵害时候,如干旱、盐、低温和病菌袭击,会启动自身的抵御机制来适应各种侵害。蔗糖非发酵相关的蛋白激酶(sucrose non-fermenting-1-related protein kinase,SnRK)是广泛存在于植物中的一类Ser/Thr蛋白激酶,参与各种胁迫信号传导通路,对植物抵御不良环境起到重要作用。植物中蔗糖非发酵相关的蛋白激酶共有38个成员,可以分为3个亚家族:SnRK1、SnRK2和SnRK3。本文主要讨论SnRK家族的研究进展,揭示SnRK家族在植物抗逆中的重要作用。 展开更多
关键词 信号通路 蔗糖非发酵相关的蛋白激酶(snrk) snrk1 snrk2 snrk3
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How Do Sugars Regulate Plant Growth and Development? New Insight into the Role of Treha Iose-6-Phosphate 被引量:28
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作者 Liam E. O'Hara Matthew J. Paul Astrid Wingler 《Molecular Plant》 SCIE CAS CSCD 2013年第2期261-274,共14页
Plant growth and development are tightly controlled in response to environmental conditions that influence the availability of photosynthetic carbon in the form of sucrose. Trehalose-6-phosphate (T6P), the precursor... Plant growth and development are tightly controlled in response to environmental conditions that influence the availability of photosynthetic carbon in the form of sucrose. Trehalose-6-phosphate (T6P), the precursor of trehalose in the biosynthetic pathway, is an important signaling metabolite that is involved in the regulation of plant growth and development in response to carbon availability. In addition to the plant's own pathway for trehalose synthesis, formation of T6P or trehalose by pathogens can result in the reprogramming of plant metabolism and development. Developmental processes that are regulated by T6P range from embryo development to leaf senescence. Some of these processes are regulated in interaction with phytohormones, such as auxin. A key interacting factor of T6P signaling in response to the environment is the protein kinase sucrose non-fermenting related kinase-1 (SnRK1), whose catalytic activity is inhibited by T6R SnRK1 is most likely involved in the adjustment of metabolism and growth in response to starvation. The transcription factor bZlP11 has recently been identified as a new player in the T6P/SnRK1 regulatory pathway. By inhibiting SnRK1, T6P promotes biosynthetic reactions. This regulation has important consequences for crop production, for example, in the developing wheat grain and during the growth of potato tubers. 展开更多
关键词 bZlP11 carbon availability plant development STARVATION sucrose non-fermenting related kinase-1 (snrk1) TREHALOSE trehalose-6-phosphate.
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