Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple

Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit quality.However,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still lacking.In the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 v1.1.The gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family expansion.The Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during domestication.Furthermore,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene subfamilies.In addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden Delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit development.Furthermore,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple fruit.The present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.

Structure and expression analysis of the sucrose synthase gene family in apple

Sucrose synthases(SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica(Md SUSs), and phylogenetic analysis revealed that the Md SUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, Md SUS1 s and Md SUS2.1 showed the highest expression in fruit, whereas Md SUS2.2/2.3 and Md SUS3 s exhibit the highest expression in shoot tips. Most Md SUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both Md SUS2.1 and Md SUS1.4 displayed opposite expression profiles. These results suggest that different Md SUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.

Molecular cloning,characterization and expression profile of the sucrose synthase gene family in Litchi chinensis

Sucrose synthase(SUS,EC 2.4.1.13)is widely considered as a key enzyme involved in plant sucrose metabolism,and the gene family encoding different SUS isozymes has been identified and characterized in several plant species.However,to date scant information about the SUS genes is available in Litchi chinensis Sonn.Here,we identified five SUS genes in litchi.These Lc SUSs shared high levels of similarity in both nucleotide and amino acid sequences.Their gene structure,phylogenetic relationships,and expression profiles were characterized.Gene structure analysis indicated that the Lc SUSs have similar exon-intron structures.Phylogenetic analysis revealed that the five members could be classified into three groups(LcSUS1 and LcSUS2 in SUSⅡ,LcSUS4 and LcSUS5 in SUSⅢ,and LcSUS3 in SUSⅠ),demonstrating evolutionary conservation in the SUS family across litchi and other plant species.The expression levels of Lc SUSs were investigated via real-time PCR in various tissues and different developmental stages of aril.For tissues and organs,Lc SUSs exhibited distinct but partially redundant expression profiles in litchi,being predominantly expressed in young leaves(sink).During aril development,the expression pattern of LcSUS1 was consistent with the trend of sugar accumulation,indicating it may play important roles in determination of sink strength in aril.Moreover,transcript levels of LcSUS2,LcSUS4,and LcSUS5 varied between cultivars with different hexose/sucrose ratios,which may regulate the sugar composition in aril.Our results provide insights into physiological functions of SUS genes in litchi,especially roles in regulating sugar accumulation in aril.

Overexpression of aspen sucrose synthase gene promotes growth and development of transgenic Arabidopsis plants

In plants, sucrose synthase (SUS) enzymes catalyze conversion of sucrose into fructose and UDP-glucose in the presence of UDP. To investigate the impact of overexpression of heterologous SUS on the growth and development of Arabidopsis, we transformed Arabidopsis plants with an overexpression vector containing an aspen SUS gene (PtrSUS1). The genomic PCR confirmed the successful integration of PtrSUS1 transgene in the Arabidopsis genome. PtrSUS1 expression in transgenic Arabidopsis plants was confirmed by RT-PCR. The SUS activity was dramatically increased in all transgenic lines examined. The three selected transgenic PtrSUS1 lines exhibited faster growth and flowered about 10 days earlier compared to untransformed controls, and also possessed 133%, 139%, and 143% SUS activity compared to controls. Both fresh weights and dry biomass yields of the whole plants from these three selected transgenic lines were significantly increased to 125% of the controls. Transgenic PtrSUS1 lines also had a higher tolerance to higher concentration of sucrose which was reflective of the increased SUS activity in transgenic versus wild-type plants. The growth differences between wild-type and transgenic plants, either in root and hypocotyl length or in fresh and dry weight of whole plant, became more pronounced on the media containing higher sucrose concentrations. Taken together, these results showed that the early flowering, faster growth and increased tolerance to higher sucrose in transgenic lines were caused by the genome integration and constitutive expression of the aspen PtrSUS1 gene in transgenic Arabidopsis.

Regulation of sucrose synthase activity and sugar yield by nitrogen in sugar beet

The content of sugar is influenced by sucrose synthase （SS） activity in roots. The effects of nitrogen level in the aminonitrate ratio on SS activity of leaves and roots, roots yield and sugar content in sugar beet were studied in the field experiment by nutrient solution culture. The results showed that SS activity in leaves was lower than that in roots. With nitrogen level increasing, SS decomposition activity enhanced, and synthesis activity reduced. SS activity was regulated by different nitrogen forms and the ratio of NO3 and NH4^＋. SS synthesis activity was enhanced as NH4^＋ increasing when NO3 ： NH4^＋≥ 1, and it decreased as increasing NH4^＋ when NO3 ： NH4^＋≤ 1, and it was the highest when NO3 ： NH4^＋=1. SS decomposition activity was enhanced as NO3- increasing. Sucrose content in root was lowed as nitrogen level increasing, but it was enhanced as NH4^＋ increasing in the same nitrogen level. Root and sugar yield were the highest in the medium nitrogen level and NO3 ： NH4^＋=1. The result in field experiment corresponded with that in the nutrient fluid culture. It provides a basis for using reasonably nitrogen fertilizer in sugar beet production.

Effect of High Temperature on Sucrose Content and Sucrose Cleaving Enzyme Activity in Rice Grain During the Filling Stage

Dynamic changes of sucrose, fructose, glucose contents and differences in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase in rice grain after flowering stage were studied under natural and high temperatures by using two japonica rice varieties Koshihikari and Sasanishiki. In rice grains, the sucrose synthase activity was higher than that of invertase, which was significantly correlated with starch accumulation rate, indicating that the sucrose synthase played an important role in sucrose degradation and starch synthesis. Under high temperature, the significant increase in grain sucrose content without any increase in fructose and glucose contents, suggested that the high temperature treatment enhanced sucrose accumulation, while diminished sucrose degradation in rice grains. Compared with the control plants, the decrease in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase with high temperature treated plants indicated that the deceleration of sucrose-degradation was related to the decrease in activities of sucrose synthase and invertase.

Variations in Carbohydrate Content and Sucrose-Metabolizing Enzymes in Tomato (<i>Solanum lycopersicum</i>L.) Stamen Parts during Pollen Maturation

The formation of mature and fertile pollen grains, taking place inside the anther, depends on supply of assimilates, in the form of sucrose, provided mainly by the leaves. Data is limited, however, with respect to the understanding of sucrose metabolism in microspores and the supporting tissues. The aims of the present work were to 1) follow the changes in total and relative concentrations of sucrose, glucose, fructose and starch in the stamen parts and microspores up until anthesis, 2) follow the activities of sucrose-metabolism-related enzymes, in the anther walls fraction and microspores of the crop plant tomato. Sucrose was found to be partially cleaved in the filament, decreasing by more than twofold in the anther wall layers and the locular fluid, and to accumulate in the mature pollen grains, constituting 80% of total soluble sugars. Thus, sucrose was both the starting sugar, supporting microspore development, and the main carbohydrate accumulated at the end of the pollen-development program. The major invertase found to be active in both the anther wall layers and in maturing microspores was cell-wall-bound invertase. High fructokinase 2 and sucrose phosphate synthase activities during pollen maturation coincided with sucrose accumulation. The potential importance of sucrose accumulation during pollen dehydration phase and germination is discussed.

Transformation of Sucrose to Starch and Protein in Rice Leaves and Grains under Two Establishment Methods

Six rice varieties, PR120, PR116, Feng Ai Zan, PR115, PAU201 and Punjab Mehak 1 were raised under aerobic and transplanting conditions to assess the effects of planting conditions on sucrose metabolising enzymes in relation to the transformation of free sugars to starch and protein in flag leaves and grains. Activities of sucrose synthase, sucrose phosphate synthase and acid invertase increased till flowering stage in leaves and mid-milky stage（14 d after flowering） in grains and thereafter declined in concomitant with the contents of reducing sugar. Under aerobic conditions, the activities of acid invertase and sucrose synthase（cleavage） significantly decreased in conjunction with the decrease in non-reducing sugars and starch content in all the varieties. Disruption of starch biosynthesis under the influence of aerobic conditions in both leaves and grains and the higher build up of sugars possibly resulted in their favoured utilization in nitrogen metabolism. Feng Ai Zan, PR115 and PR120 maintained higher levels of sucrose synthase enzymes in grains and leaves and contents of metabolites（amino acid, protein and non-reducing sugar） under aerobic conditions, while PR116, Punjab Mehak 1 and PAU201 performed better under transplanting conditions, thus showing their adaptation to environmental stress. Yield gap between aerobic and transplanting rice is attributed primarily to the difference in sink activity and strength. Overall, it appear that up-regulation of sucrose synthase（synthesis） and sucrose phosphate synthase under aerobic conditions might be responsible in enhancing growth and productivity of rice varieties.

Polyphosphate Accelerates Transformation of Nonstructural Carbohydrates to Improve Growth of ppk-Expressing Transgenic Rice in Phosphorus Deficiency Culture

Crop yield and quality are often limited by the amount of phosphate fertilizer added to infertile soils,a key limiting factor for sustainable development in modern agriculture.The polyphosphate kinase(ppk)gene-expressing transgenic rice with a single-copy line(ETRS)is constructed to improve phosphate fertilizer utilization efficiency for phosphorus resource conservation.To investigate the potential mechanisms of the increased biomass in ETRS in low phosphate culture,ETRS was cultivated in a low inorganic phosphate(Pi)culture medium(15μmol/L Pi,LP)and a normal Pi culture medium(300μmol/L Pi,CP),respectively.After 89 d of cultivation in different concentrations of phosphate culture media,the total phosphorus,polyphosphate(polyP),biomass,photosynthetic rate,nonstructural carbohydrate(NSC)contents,related enzyme activities,and related gene expression levels were analyzed.The results showed that ETRS had a high polyP amount to promote the photosynthetic rate in LP,and its biomass was almost the same as the wild type(WT)in CP.The NSC content of ETRS in LP was higher than that of WT in LP,but slightly lower than that of WT in CP.PolyP notably promoted the sucrose phosphate synthase activities of ETRS and significantly down-regulated the expression levels of sucrose transporter genes(OsSUT3 and OsSUT4),resulting in inhibiting the transport of sucrose from shoot to root in ETRS.It was concluded that polyP can stimulate the synthesis of NSCs in LP,which improved the growth of ETRS and triggered the biological activities of ETRS to save phosphate fertilizer.Our study provides a new way to improve the utilization rate of phosphate fertilizer in rice production.

Sucrose enhances the chromogenic ability of Staphylococcus xylosus by improving nitric oxide synthase activity

In this paper,the effect of different concentrations of sucrose stress on color formation of the Staphylococcus xylosus was investigated.The results showed that the highest a*value and the best coloring effect similar to those of nitrite were observed after the addition of 0.05 g/mL sucrose to stress the S.xylosus.UV-Vis and electron spin resonance spectra analysis showed that production of coloring product Mb-NO was significantly enhanced after 0.05 g/mL sucrose stress.The growth curve,reactive oxygen content,cell cycle,nitric oxide synthase(NOS)activity,zeta potential,cell size,and protein composition of S.xylosus were investigated to reveal the mechanism of sucrose stress to enhance the coloring effect of the strain.The result showed that sucrose inhibited the growth of S.xylosus,which changed the physiological state by activating the oxidative stress response.The stress altered the rate of intracellular metabolism of S.xylosus by delaying the cell cycle and increasing cell surface zeta potential and cell particle size.These changes altered the protein composition of the cells and significantly enhanced the activity of intracellular NOS,which could improve the chromogenic ability of S.xylosus.This study will provide theoretical support for sucrose stress on S.xylosus to enhance its coloring effect,and sucrose stress for S.xylosus might be a promising biological alternative to nitrite in meat products.

Overexpression of a Potato Sucrose Synthase Gene in Cotton Accelerates Leaf Expansion, Reduces Seed Abortion, and Enhances Fiber Production

Sucrose synthase （Sus） is a key enzyme in the breakdown of sucrose and is considered a biochemical marker for sink strength, especially in crop species, based on mutational and gene suppression studies. It remains elusive, however, whether, or to what extent, increase in Sus activity may enhance sink development. We aimed to address this question by expressing a potato Sus gene in cotton where Sus expression has been previously shown to be critical for normal seed and fiber development. Segregation analyses at T1 generation followed by studies in homozygous progeny lines revealed that increased Sus activity in cotton （1） enhanced leaf expansion with the effect evident from young leaves emerging from shoot apex; （2） improved early seed development, which reduced seed abortion, hence enhanced seed set, and （3） promoted fiber elongation. In young leaves of Sus overexpressing lines, fructose concentrations were significantly increased whereas, in elongating fibers, both fructose and glucose levels were increased. Since hexoses contribute little to osmolality in leaves, in contrast to developing fibers, it is concluded that high Sus activity promotes leaf development independently of osmotic regulation, probably through sugar signaling. The analyses also showed that doubling the Sus activity in 0-d cotton seeds increased their fresh weight by about 30%. However, further increase in Sus activity did not lead to any further increase in seed weight, indicating an upper limit for the Sus overexpression effect. Finally, based on the observed additive effect on fiber yield from increased fiber length and seed number, a new strategy is proposed to increase cotton fiber yield by improving seed development as a whole, rather than solely focusing on manipulating fiber growth.

高粱可溶性糖含量与SS、SPS酶活性的相关性研究

以5个高粱品系和1个杂交种为试材,研究了不同生长发育时期高粱可溶性糖含量与SS、SPS酶活性的变化规律及相关性。结果表明,SS与可溶性糖含量在生长后期呈负相关,SPS与可溶性糖含量呈正相关。茎秆可溶性糖含量一直呈上升趋势,在完熟期达到最大值,此时正是高粱的最佳收获时期。

甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究

以工艺成熟期甘蔗品种GT28(早熟)和ROC22(早中熟)+1叶和不同节间为材料,分析SPS、SS合成和SS分解方向酶活性与节间糖分积累的相关性。结果表明,+1叶中SPS、SS合成和分解方向的酶活性比蔗茎中高,说明+1叶代谢活跃,是蔗茎糖分不断积累的物质基础。节间SPS酶活性与节间锤度、可溶性总糖和蔗糖含量都正相关,GT28节间SPS酶活性比ROC22中高。节间SS合成方向酶活性与锤度和蔗糖含量都负相关,GT28中SS合成方向酶活性比ROC22中低。而SS分解方向酶活性与ROC22节间锤度、可溶性糖和蔗糖含量都呈负相关,与GT28中呈正相关,未达到显著水平。说明成熟期节间SPS和SS合成方向酶活性提高有利于蔗糖的积累,而随着节间成熟,蔗糖含量可能是促使SS酶活性由合成方向向分解方向转化的一个重要调节因子。

不同种植密度对甜高粱糖分积累及SS、SPS活性的影响

对2个甜高粱品种XT-2、T601设置7个不同种植密度处理,分析了甜高粱不同生育阶段总糖含量、还原糖含量、蔗糖含量、SS和SPS活性的变化。2个品种的总糖含量随生长进程均升高,成熟期达最高值。其还原糖的变化趋势不一致,XT-2呈升高-平缓-降低的趋势,T601呈升高-降低的趋势。2个品种在整个生育期蔗糖含量均呈升高的趋势,成熟期达最高值。T601除密度处理B6(6170株hm–2)外其他处理SS活性均呈升高-降低-升高的趋势,XT-2无明显规律。XT-2除密度处理B2(11 110株hm–2)、B4(7929株hm–2)外其他处理SPS活性均呈升高-降低-升高的趋势,T601无明显规律。综上所述,甜高粱品种XT-2最适于糖分积累的密度为9250株hm–2,成熟期总糖含量达14.2%(鲜基),T601的最适密度为11 110株hm–2,成熟期总糖含量达14.31%(鲜基),作为积累糖的原料,后者为最佳选择。完熟期SS活性下降、SPS活性上升有利于糖的积累,为最佳收获时期。

小麦SUS基因家族鉴定与生物信息学分析

【目的】对小麦蔗糖合成酶(sucrose synthase,SUS)基因家族进行鉴定和生物信息学分析,为探究小麦SUS(TaSUS)基因家族的作用机制提供理论参考。【方法】采用生物信息学方法在小麦全基因组上鉴定TaSUS基因家族成员,并对其系统进化关系、染色体位置、基因结构、保守结构域、启动子顺式作用元件和基因表达模式进行分析。【结果】在小麦基因组中共鉴定到分布于14条染色体上的24个TaSUS基因,可分为3个亚组。TaSUS基因含有多个外显子,但部分基因缺失非翻译区结构。TaSUS基因家族成员启动子区域包含45种顺式作用元件,涉及植物生长发育和逆境胁迫响应。大多数TaSUS基因在小麦穗中显著表达,在叶、茎和根中的相对表达量较低。【结论】研究结果有助于了解小麦SUS基因家族的进化,为后期小麦SUS基因家族的生物功能研究奠定理论基础。

不同播种期下甜高粱秸秆SS、SPS酶活性的研究

【目的】以新高粱3号(XT-2)和新高粱9号(T601)为研究对象,分析不同播期甜高粱不同生育阶段茎秆SS和SPS酶活性的变化规律及相关性。【方法】在5个不同播期条件下进行了蔗糖和酶活性测定试验。【结果】茎秆的蔗糖含量随着生育期不断升高,成熟期达到最高值。不同品种和不同播期秸秆蔗糖含量有差异,新高粱3号第二播期的蔗糖含量最高,高达7.66%,第五播期最低,仅为1.87%。对于新高粱9号来说第三播期蔗糖含量最高,达9.17%,第二播期最低,仅为6.19%。不同播期和不同品种之间秸秆蔗糖含量与SS、SPS酶活性的相关趋势有差异。除新高粱3号第一播期、第三播期的蔗糖含量与SS酶活性呈极显著相关外,其余播期的蔗糖含量与SS酶活性均呈相关性不显著。不同播期下秸秆蔗糖含量与SPS酶活性均呈相关不显著。【结论】早播时新高粱3号蔗糖含量比较高,晚播时新高粱9号蔗糖含量高。因此新疆,尤其是在北疆新高粱3号糖分积累的最适播期为5月初旬,新高粱9号最适播期为5月中旬。

大白菜SUS基因家族鉴定与节律表达分析

植物体内的蔗糖合酶(SUS)是一种糖基转移酶,它参与调控蔗糖的合成与运输,研究大白菜SUS基因家族的表达为揭示糖代谢的分子调控机制提供了重要基础。本研究利用生物信息学方法,进行大白菜SUS基因家族成员鉴定、理化性质、系统进化关系、蛋白基序、基因结构和物种内(间)共线性分析;利用RNA-seq技术,获得SUS家族基因的相对表达量,分析大白菜SUS家族基因的节律表达模式。结果表明:大白菜中包含13个SUS基因,均含有SUS结构域;大白菜SUS基因家族可分为3个亚家族(SUSⅠ,SUSⅡ,SUSⅢ);SUSⅡ亚家族的基因motif保守基序种类最多;多数基因含有11~14个CDS序列。大白菜SUS基因家族物种内共线性分析有4对基因参与了大片段重复事件,为大白菜进化提供动力。大白菜与拟南芥的SUS基因家族物种间共线性分析,物种间共构建了13对同源基因,其中有多个“一对多,多对一”的同源基因关系,均属于SUS基因家族的基因对有10个。对SUS家族基因的KEGG和GO功能富集分析,发现富集在淀粉与蔗糖代谢、蔗糖合酶活性通路等方面上。分析SUS家族基因的节律表达模式,发现BraSUSs基因中BraA10g019840.3C基因表达呈现节律性,而其余基因多在夜晚高表达。本研究结果可为大白菜SUSs基因功能解析及蔗糖代谢调控机制研究奠定理论基础。

蔗糖合成酶PavSS1调控甜樱桃果实成熟的功能分析

【目的】明确甜樱桃PavSS基因家族在果实成熟过程中的功能。【方法】利用qRT-PCR技术分析了7个蔗糖合成酶基因PavSS1~7在果实发育过程中的表达情况,并分析了蔗糖对PavSS家族基因表达的影响,利用甜樱桃果实的VIGS技术分析了PavSS1与PavSS6基因影响果实成熟的功能。【结果】PavSS1和PavSS2基因在甜樱桃果实发育和成熟过程中均表达,且PavSS1基因表达量是PavSS2基因的10倍以上;PavSS1和PavSS2在果实发育前期表达量逐渐升高,后期表达量逐渐降低。PavSS3和PavSS5基因在整个果实发育期均低表达,且表达量差异不显著。PavSS4和PavSS7基因在果实发育前期表达量高,后期表达量低。PavSS6基因在果实发育初期低表达,随后表达量逐渐升高,并维持较高水平。外施蔗糖显著下调了PavSS1和PavSS6基因的表达,而PavSS2、PavSS3、PavSS4、PavSS5和PavSS7基因的表达变化不显著。沉默甜樱桃果实PavSS1基因抑制了甜樱桃果实成熟,包括果实硬度、ABA含量、花色苷含量和可溶性糖含量发生显著变化以及成熟相关基因的表达量显著下调;而沉默PavSS6基因的甜樱桃果实与空载对照相比,没有显著变化。【结论】PavSS1基因可能是控制甜樱桃果实蔗糖代谢的关键基因,参与了甜樱桃果实成熟过程的调控。

甜高粱露天贮藏SS、SPS酶活性与蔗糖代谢的研究(英文)

【目的】为明确甜高粱茎秆贮存过程中蔗糖降解的关键酶,以期通过对蔗糖降解关键酶的分子调控解决甜高粱茎秆贮存过程中蔗糖易降解的难题。【方法】以新高粱9号(T601)为试验材料,研究了井型去叶去穗、井型带叶去穗堆放贮藏方式下茎秆蔗糖含量与SS、SPS酶活性的变化规律及相关性。【结果】在贮藏期间SPS、SS与蔗糖含量相关性都不显著,蔗糖含量当贮藏达到5个月时,急速下降至最低。【结论】带叶去穗井型贮藏较去叶去穗井型贮藏更有利于甜高粱秸秆蔗糖含量的保存,但是霉变腐烂成为带叶去穗型贮藏的限制性因素,贮藏堆放高度应控制在2 m以下。

东方泽泻实时荧光定量PCR内参基因的筛选及AoSS基因的组织表达特性分析

【目的】筛选适用于东方泽泻实时荧光定量PCR(qRT-PCR)的内参基因,并分析其鲨烯合酶基因(AoSS)的表达特性,为后续研究东方泽泻原萜烷型三萜生物合成调控及诱导机制打下基础。【方法】从东方泽泻转录组中选取内参基因18S rRNA、EF1α、GAPDH、ACT、UBQ和UBC,应用qRT-PCR检测其在不同组织和激素处理下的表达情况,并借助Delta CT、GeNorm、NormFinder、BestKeeper和RefFinder对其表达稳定性进行分析评价。以筛选到的内参基因分析AoSS基因在不同组织中的表达情况。【结果】18S r RNA、GAPDH和EF1α基因在东方泽泻不同组织的表达稳定性较高,但18S rRNA、UBC和EF1α基因在不同激素处理下的表达稳定性均较高。以18S rRNA、GAPDH和EF1α基因为内参分析AoSS基因在东方泽泻不同组织中的表达量情况,结果显示,AoSS基因在东方泽泻不同组织中均有表达,其表达情况基本一致,均在东方泽泻块茎中表达量最高,其次是叶柄和叶,而在根中表达量最低。【结论】18S rRNA、GAPDH和EF1α基因是研究东方泽泻不同组织中基因表达分析的首选内参基因;18S rRNA、EF1α和UBC基因是研究激素处理下基因表达的首选内参基因。AoSS基因的表达具有组织特异性,其可能在东方泽泻的生长发育中发挥重要的调控功能。