[Objectives]The paper was to explore the occurrence and mixed infection of sugarcane bacilliform virus disease in Hainan sugarcane-growing area.[Methods]A total of 348 sugarcane leaf samples were collected from 7 suga...[Objectives]The paper was to explore the occurrence and mixed infection of sugarcane bacilliform virus disease in Hainan sugarcane-growing area.[Methods]A total of 348 sugarcane leaf samples were collected from 7 sugarcane-growing areas in Hainan Province.Molecular detection of sugarcane bacilliform virus(SCBV)was carried out by PCR using specific primers.[Results]SCBV was detected in 244 out of 348 sugarcane samples,with an average detection rate of 70.11%.The highest detection rate was 76.66%in the Danzhou sugarcane-growing area,while the lowest was 57.14%in the Baisha sugarcane-growing area.The SCBV-positive samples were subjected to testing for SCYLV,SCSMV,SrMV,and SCMV,respectively.The results indicated that 106 out of 244 positive samples exhibited a single infection with SCBV,while 138 samples exhibited mixed infections with SCBV and other sugarcane viruses.The proportion of mixed infections among the SCBV-positive samples was as high as 56.56%.Among the various types of mixed infections,two-virus and three-virus mixed infections were the most prevalent.[Conclusions]SCBV has emerged as a significant threat to the secure production of sugarcane in the Hainan sugarcane-growing region.It presents an explosive infection in the Hainan sugarcane-growing region and frequently combines with other sugarcane viruses to infect sugarcane.The findings of this study will provide a theoretical foundation for the prevention and control of sugarcane bacilliform virus disease.展开更多
To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collect...To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.展开更多
Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Bur...Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.展开更多
Sugarcane bacilliform virus(SCBV) was detected by PCR from sugarcane showing chlorosis and mottle symptom from Kaiyuan,Yunnan Province.Part sequence of replicase gene of the isolate SCBV-Kaiyuan was determined.Sequenc...Sugarcane bacilliform virus(SCBV) was detected by PCR from sugarcane showing chlorosis and mottle symptom from Kaiyuan,Yunnan Province.Part sequence of replicase gene of the isolate SCBV-Kaiyuan was determined.Sequence analysis indicated that the 589 bp of SCBV-Kaiyuan shared identities of 73.2%-74.0% and 83.1%-84.1% at nucleotide and amino acid levels with SCBV-Australia respectively,66.7%-68.4% and 65.6%-67.7% with SCBV-Morocco.The quality and yield of the sugarcane infected with SCBV-Kaiyuan was also investigated.The juice extraction,sucrose content,gravity purity and average stalk weight were decreased 1.55%,1.24%,2.22% and 0.26 kg in plants infected with SCBV-Kaiyuan,but reducing sugar was increased by 0.21% in infected plants.展开更多
甘蔗杆状病毒(sugarcane bacilliform virus,SCBV)是广泛分布于世界甘蔗种植区的严重危害甘蔗种植的植物病毒之一。研究SCBV编码的病毒蛋白功能对于了解该病毒致病机制及抗病育种具有重要意义。本实验室前期从云南勐海的一株Badila甘蔗...甘蔗杆状病毒(sugarcane bacilliform virus,SCBV)是广泛分布于世界甘蔗种植区的严重危害甘蔗种植的植物病毒之一。研究SCBV编码的病毒蛋白功能对于了解该病毒致病机制及抗病育种具有重要意义。本实验室前期从云南勐海的一株Badila甘蔗中分离获得了SCBV的全长基因组序列,并开展了其开放阅读框2(open reading frame 2, ORF2)编码的未知蛋白P2的功能研究。通过原核表达SCBV P2蛋白并经体外核酸亲和结合试验分析,发现SCBV编码的P2蛋白能以序列非特异性方式与同源或异源核酸亲和结合。进一步对P2蛋白中预测的coiled-coil like domain及C-端保守的脯氨酸、赖氨酸富集区域进行缺失及点突变分析,以及凝胶阻滞试验发现,随着C-端逐步截短,P2突变体蛋白与核酸结合的活性也逐渐减弱,其C-端保守的含脯氨酸、赖氨酸的21个氨基酸(amino acids,aa)对P2蛋白与核酸结合是必需的。同时,在P2蛋白与核酸的相互作用中,coiled-coil like domain被证明是不可或缺的。进一步酵母双杂交实验证明P2蛋白能与自身互作,且P2 C-端保守的21个氨基酸及coiled-coil like domain的缺失或替换直接影响了P2蛋白的自身互作。综上所述,本研究发现SCBV编码的P2蛋白是一个序列非特异性的核酸结合蛋白,且coiled-coil like domain和C-端保守的富含脯氨酸、赖氨酸区域通过影响其自身互作而影响其核酸结合活性。研究结果为进一步深入研究P2蛋白功能奠定了基础。展开更多
基金Supported by Hainan Provincial Natural Science Foundation of China(321QN313,323MS103)Earmarked Fund for China Agricultural Research System(CARS-17).
文摘[Objectives]The paper was to explore the occurrence and mixed infection of sugarcane bacilliform virus disease in Hainan sugarcane-growing area.[Methods]A total of 348 sugarcane leaf samples were collected from 7 sugarcane-growing areas in Hainan Province.Molecular detection of sugarcane bacilliform virus(SCBV)was carried out by PCR using specific primers.[Results]SCBV was detected in 244 out of 348 sugarcane samples,with an average detection rate of 70.11%.The highest detection rate was 76.66%in the Danzhou sugarcane-growing area,while the lowest was 57.14%in the Baisha sugarcane-growing area.The SCBV-positive samples were subjected to testing for SCYLV,SCSMV,SrMV,and SCMV,respectively.The results indicated that 106 out of 244 positive samples exhibited a single infection with SCBV,while 138 samples exhibited mixed infections with SCBV and other sugarcane viruses.The proportion of mixed infections among the SCBV-positive samples was as high as 56.56%.Among the various types of mixed infections,two-virus and three-virus mixed infections were the most prevalent.[Conclusions]SCBV has emerged as a significant threat to the secure production of sugarcane in the Hainan sugarcane-growing region.It presents an explosive infection in the Hainan sugarcane-growing region and frequently combines with other sugarcane viruses to infect sugarcane.The findings of this study will provide a theoretical foundation for the prevention and control of sugarcane bacilliform virus disease.
基金Supported by Special Fund of China Agricultural(Sugar)Industry Research System(CARS-170301)Basal Research Fund of Central Public Welfare Research Institutes(1630052016010)
文摘To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.
文摘Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.
文摘Sugarcane bacilliform virus(SCBV) was detected by PCR from sugarcane showing chlorosis and mottle symptom from Kaiyuan,Yunnan Province.Part sequence of replicase gene of the isolate SCBV-Kaiyuan was determined.Sequence analysis indicated that the 589 bp of SCBV-Kaiyuan shared identities of 73.2%-74.0% and 83.1%-84.1% at nucleotide and amino acid levels with SCBV-Australia respectively,66.7%-68.4% and 65.6%-67.7% with SCBV-Morocco.The quality and yield of the sugarcane infected with SCBV-Kaiyuan was also investigated.The juice extraction,sucrose content,gravity purity and average stalk weight were decreased 1.55%,1.24%,2.22% and 0.26 kg in plants infected with SCBV-Kaiyuan,but reducing sugar was increased by 0.21% in infected plants.
文摘甘蔗杆状病毒(sugarcane bacilliform virus,SCBV)是广泛分布于世界甘蔗种植区的严重危害甘蔗种植的植物病毒之一。研究SCBV编码的病毒蛋白功能对于了解该病毒致病机制及抗病育种具有重要意义。本实验室前期从云南勐海的一株Badila甘蔗中分离获得了SCBV的全长基因组序列,并开展了其开放阅读框2(open reading frame 2, ORF2)编码的未知蛋白P2的功能研究。通过原核表达SCBV P2蛋白并经体外核酸亲和结合试验分析,发现SCBV编码的P2蛋白能以序列非特异性方式与同源或异源核酸亲和结合。进一步对P2蛋白中预测的coiled-coil like domain及C-端保守的脯氨酸、赖氨酸富集区域进行缺失及点突变分析,以及凝胶阻滞试验发现,随着C-端逐步截短,P2突变体蛋白与核酸结合的活性也逐渐减弱,其C-端保守的含脯氨酸、赖氨酸的21个氨基酸(amino acids,aa)对P2蛋白与核酸结合是必需的。同时,在P2蛋白与核酸的相互作用中,coiled-coil like domain被证明是不可或缺的。进一步酵母双杂交实验证明P2蛋白能与自身互作,且P2 C-端保守的21个氨基酸及coiled-coil like domain的缺失或替换直接影响了P2蛋白的自身互作。综上所述,本研究发现SCBV编码的P2蛋白是一个序列非特异性的核酸结合蛋白,且coiled-coil like domain和C-端保守的富含脯氨酸、赖氨酸区域通过影响其自身互作而影响其核酸结合活性。研究结果为进一步深入研究P2蛋白功能奠定了基础。
