Application of Saccharomyces boulardii in combination with sulfasalazine in ulcerative colitis patients demonstrates significant effectiveness

BACKGROUND Ulcerative colitis(UC)is a complex inflammatory bowel disease,and its etiology and pathogenesis remain incompletely elucidated.AIM To analyze the effects of Saccharomyces boulardii in combination with sulfasalazine on intestinal microbiota and intestinal barrier function in patients with UC.METHODS A retrospective analysis of clinical data from 127 UC patients admitted to our hospital between January 2021 and January 2023 was conducted.All patients met complete inclusion and exclusion criteria.Based on the treatment interventions received,they were divided into a control group(n=63)and an observation group(n=64).Both groups of patients received routine treatment upon admission.The control group received sulfasalazine in addition to routine interventions,while the observation group received a combination of Saccharomyces boulardii on the basis of the control group’s treatment.The clinical efficacy,improvement in symptoms,modified Baron endoscopic scores,quality of life“inflammatory bowel disease questionnaire(IBDQ)”,levels of intestinal microbial indicators(such as Lactobacillus,Bifidobacterium,Enterococcus,and Escherichia coli),intestinal mucosal barrier function indicators[diamine oxidase(DAO),lipopolysaccharide(LPS),D-lactic acid(D-LA)],and adverse reaction occurrences were compared between the two groups.RESULTS(1)Clinical efficacy:The total effective rate in the control group was 79.37%,while in the observation group,it was 93.75%,significantly higher than that of the control group(P<0.05);(2)Improvement in symptoms:The observation group showed significantly lower relief time for abdominal pain,diarrhea,rectal bleeding,fever symptoms,and mucosal healing time compared to the control group(P<0.05);(3)Baron endoscopic scores and IBDQ scores:Before treatment,there was no significant difference in Baron endoscopic scores and IBDQ scores between the two groups(P>0.05).However,after treatment,the observation group showed significantly lower Baron endoscopic scores and higher IBDQ scores compared to the control group(P<0.05);(4)Levels of intestinal microbial indicators:Before treatment,there was no significant difference in the levels of Lactobacillus,Bifidobacterium,Enterococcus,and Escherichia coli between the two groups(P>0.05).After treatment,the levels of Lactobacillus and Bifidobacterium in the observation group were significantly higher than those in the control group,while the levels of Enterococcus and Escherichia coli were significantly lower than those in the control group(P<0.05);(5)Levels of intestinal mucosal barrier function indicators:Before treatment,there was no significant difference in the levels of DAO,LPS,and D-LA between the two groups(P>0.05).However,after treatment,the levels of DAO,LPS,and D-LA in the observation group were significantly lower than those in the control group(P<0.05);and(6)Occurrence of adverse reactions:The incidence of adverse reactions in the control group was 9.52%,while in the observation group,it was 10.94%.There was no significant difference in the occurrence of adverse reactions between the two groups(P>0.05).CONCLUSION The application of Saccharomyces boulardii in combination with sulfasalazine in UC patients demonstrates significant effectiveness.Compared to sole sulfasalazine intervention,the combined application of Saccharomyces boulardii further promotes the relief of relevant symptoms in patients,alleviates intestinal mucosal inflammation,and improves the quality of life.Its action may be related to rectifying the imbalance in intestinal microbiota and improving intestinal mucosal barrier function.Moreover,the combined use of Saccharomyces boulardii does not increase the risk of adverse reactions in patients,indicating a higher level of medication safety and advocating for its clinical promotion and application.

ADS启明星次临界装置退役源项调查研究

加速器驱动次临界系统(ADS)启明星次临界实验装置是我国首座退役的次临界装置,针对其退役源项调查,建立一套适用于临界装置退役的源项调查方法。首先对ADS启明星次临界实验装置进行详细的源项资料调查,采用ORIGEN程序和蒙特卡罗程序相结合的方法给出堆本体各部件活化产生的放射性核素及其源强和比活度,通过现场测量和取样分析调查确定堆本体结构部件的放射性源项,本研究成果对其他临界装置开展退役源项调查具有借鉴意义。

碳酸钙加维生素AD治疗小儿佝偻病临床效果及骨密度分析

探讨基于临床罹患小儿佝偻病患者,采取碳酸钙+维生素AD治疗手段,分析其对患者骨密度等方面的影响。方法 遴选时段介于2022年1月-2024年12月,随机选取以本院就诊的80例罹患小儿佝偻病患儿作为研究治疗群体,借助随机法落实分组操作,分为2组分:对照组、观察组,每组各自40例,对照组则单一服用复方碳酸钙泡腾颗粒治疗,观察组则服用复方碳酸钙泡腾颗粒+维生素AD软胶囊联合治疗,比较组间患者的骨密度、血清生化指标、疗效率、不良反应。结果 两组之间的桡骨和尺骨骨密度测量统计,显示在治疗前数据相当(P>0.05),经治疗1个月后,观察组桡骨骨密度测得值、尺骨骨密度测得值均显示高于对照组(P<0.05)。组间血清生化指标实施比较,在开展治疗之前数据具备相似性(P>0.05),经治疗之后,观察组的血清磷、血清钙、25-羟基维生素D3化验值均显示高于对照组(P<0.05)。两组的疗效率对比,观察组总有效率为更高水平。不同组之间的不良反应展开统计,显示数据相当(P>0.05)。结论 基于临床罹患小儿佝偻病患者,采取碳酸钙+维生素AD治疗手段,相较单一服用复方碳酸钙泡腾颗粒治疗更具影响意义,不但可助于提高骨密度,还可助于改善血清钙磷、25-羟基维生素D3水平,且疗效率高,治疗安全。

维生素AD联合葡萄糖酸锌治疗小儿轮状病毒性肠炎疗效及对血清IL-6和INF-γ的影响

目的:探究维生素AD联合葡萄糖酸锌治疗小儿轮状病毒性肠炎疗效及对血清白介素(IL)-6和γ干扰素(INF-γ)的影响。方法:选取安阳市妇幼保健院收治的108例轮状病毒性肠炎患儿分为对照组与联合组,每组54例。对照组予以葡萄糖酸锌治疗,联合组在对照组基础上联合维生素AD治疗,均治疗5 d。比较两组临床疗效、症状改善时间、免疫功能指标[免疫球蛋白(Ig)A、IgG、IgM]、炎症指标(IL-6、INF-γ)间差异,分析两组用药安全性。结果:联合组总有效率高于对照组(P<0.05);联合组高烧、腹泻、腹痛及呕吐等症状的缓解时间及粪常规检查恢复正常时间均显著低于对照组(P<0.05);治疗后,两组血清IL-6、INF-γ水平显著降低,且联合组显著低于对照组(P<0.05);治疗后,两组IgA、IgG、IgM均显著高于治疗前,且联合组均显著高于对照组(P<0.05)。结论:维生素AD联合葡萄糖酸锌治疗小儿轮状病毒性肠炎疗效较单行葡萄糖酸锌治疗效果更佳,可快速缓解症状,且具有用药安全性,其作用机制可能与改善炎症反应,增强免疫功能相关。

[Zn(sulfasalazine)_2(pyridine)]

In compound [Zn(sulfasalazine)2(pyridine)] (1), each zinc atom not only coordinates to nitrogen atom of pyridine, but also is connected with four oxygen atoms from carboxylic acid groups of four sulfasalazine ligands, to result in the formation of a distorted tetragonal pyramid. In addition, each sulfasalazine ligand coordinates to two zinc atoms through its two oxygen atoms of carboxylic acid group to afford a 1D chain. CCDC: 274842.

ADS-B信号在对流层大气波导中的传播性能

对流层大气环境存在特殊大气结构“大气波导”,可形成电波超视距传播或产生雷达盲区等。目前,通过雷达、GNSS信号等可以对大气波导进行一定程度的反演探测,但均存在一定的不足。ADS-B信号具有应用范围广、信号密度大、实时性高等特点,为此提出利用ADS-B信号受到不同大气环境影响后能量损耗不同的特点对ADS-B信号与对流层大气环境关联性进行分析研究。以武汉地区ADS-B信号数据为例,基于抛物方程传播理论对路径损耗进行仿真分析,并进行了实验验证。结果表明:ADS-B接收信号与仿真结果存在线性相关性并给出线性表述,为后续利用ADS-B信号反演大气波导提供参考依据。

基于ADE优化的IPMSM全速域无传感器控制

为了实现内置式永磁同步电机(IPMSM)全速域的无传感器控制和切换速域的平滑过渡,提出了一种基于自适应差分进化(ADE)算法优化的复合控制方法。分别在零低速域、中高速域采用旋转高频电压注入法和滑模观测器法来对电机转速和转子位置进行估算,并在切换速域采用基于ADE算法的权重系数优化法来实现上述两种控制方法的平滑切换,从而实现IPMSM全速域无传感器控制。仿真结果表明:提出的复合控制方法能够实现电机全速域的无感控制和切换速域的平滑过渡,且具有良好的稳定性。

儿童维生素AD滴剂,这样吃才对

众所周知,维生素A、D对孩子的生长发育至关重要。而补充维生素A、D,最好选择专门的维生素AD制剂,比如维生素AD滴剂。但许多家长对其又不甚了解,有诸多疑问。以下,笔者将一一进行解答。

New approach of medicinal herbs and sulfasalazine mixture on ulcerative colitis induced by dextran sodium sulfate

BACKGROUND Sulfasalazine has been used as a standard-of-care in ulcerative colitis for decades,however,it results in severe adverse symptoms,such as hepatotoxicity,blood disorders,male infertility,and hypospermia.Accordingly,the new treatment strategy has to enhance pharmacological efficacy and stimultaneously minimize side effects.AIM To compare the anti-inflammatory action of sulfasalazine alone or in combination with herbal medicine for ulcerative colitis in a dextran sodium sulfate(DSS)-induced colitis mouse model.METHODS To induce ulcerative colitis,mice received 5%DSS in drinking water for 7 d.Animals were divided into five groups(n=9 each)for use as normal(non-DSS),DSS controls,DSS+sulfasalazine(30 mg/kg)-treatment experimentals,DSS+sulfasalazine(60 mg/kg)-treatment experimentals,DSS+sulfasalazine(30 mg/kg)+Citrus unshiu peel and Bupleuri radix mixture(30 mg/kg)(SCPB)-treatment experimentals.RESULTS The SCPB treatment showed an outstanding effectiveness in counteracting the ulcerative colitis,as evidenced by reduction in body weight,improvement in crypt morphology,increase in antioxidant defenses,down-regulation of proinflammatory proteins and cytokines,and inhibition of proteins related to apoptosis.CONCLUSIONSCPB may represent a promising alternative therapeutic against ulcerative colitis,without inducing adverse effects.

Characteristics and therapeutic efficacy of sulfasalazine in patients with mildly and moderately active ulcerative colitis

AIM: To investigate the characteristics and short-term efficacy of sulfasalazine (SASP) in patients with mildly and moderately active ulcerative colitis (UC).METHODS: Two hundred and twenty-eight patients with mildly and moderately active UC were recruited, 106patients in 1993-1995, and 122 patients in 2000-2002,they were assigned as the 1990s group (n = 106) and the 2000s group (n = 122), prospectively. The general characteristics, clinical manifestations, colonoscopic and histological data were compared between the two groups.The short-term efficacy and safety of SASP 3 g per d were evaluated.RESULTS: Between 2000s and 1990s groups, the gender ratio of men to women was 1:1.18 and 1:1.04, 57.4%and 50.9% of the patients were between 30 and 49 years old. The gender ratio and age of UC patients were not significantly different. The total course of 50.0% and 37.1% of UC patients was less than 1 year (P＜0.05), 10.6% and 31.2% of the cases had a duration of more than 5 years (P＜0.05) in 2000s and 1990s groups, respectively. The most common clinical type was first episode in 2000s group and chronic relapse in 1990s group. The patients showed a higher frequency of abdominal pain and tenderness in 1990s group than in 2000s group. Erosions were found in 84.4% and 67.9% of patients in 2000s and 1990s groups (P＜0.05). Rough and granular mucosa (67.9%vs43.4%, P＜0.05)and polyps (47.2% vs 32.8%, P＜0.05)were identified in 1990s group more than in 2000s group.There were no significant differences in clinical, colonoscopic and histological classifications. After SASP (1 g thrice per d) treatment for 6 wk, the clinical, colonoscopic and histological remission rates were 71.8%, 21.8% and 16.4%,respectively. In 79 patients with clinical remission, 58.2%and 67.1% remained grade 1 in colonoscopic and histological findings, respectively. The overall effects in first episode type (complete remission in 10, 18.9%, partial remission in 28, 52.8%, and improvement in 9, 17.0%) were better than in chronic relapse type (complete remission in 3,7.5%; partial remission in 16, 40.0%; and improvement in 15, 37.5%) and chronic persistent type (complete remission in 1, 5.9%; partial remission in 6, 35.3%; and improvement in 6, 35.3%) respectively (P＜0.05). In 110patients treated with SASP, 18 patients (16.4%) had adverse reactions. Except for two cases of urticaria and one case of WBC decrease, none of the patients had to stop the treatment because of severe adverse reactions.CONCLUSION: Patients with mildly and moderately active UC in 2000s group had a shorter disease course, milder clinical manifestations, more first episode type and higher frequency of acute mucosal lesions in colonoscopy than in 1990s group. The patients in 1990s group had higher proportion of chronic relapse type and chronic mucosal change in colonoscopy than in 2000s group. The shortterm efficacy of SASP could be mainly remission of clinical manifestations. But more than half of the patients still had light inflammation in colonoscopy and histology. The overall effects of SASP in first episode type were better than those in other types. SASP was a safe and effective drug to treat mildly and moderately active UC.

Effects of 5-fluouracil combined with sulfasalazine on human pancreatic carcinoma cell line BxPC-3 proliferation and apoptosis in vitro

BACKGROUND:Most pancreatic carcinomas are clinically insensitive to chemotherapeutics.The exact mechanisms of their apoptosis and multiple drug resistance are obscure at present.This study was undertaken to explore the influence of chemotherapy on anti-proliferation,apoptosis and the cell cycle,and lay a fundamental basis for further research into the apoptotic mechanisms and prevention of multiple drug resistance in pancreatic carcinoma. METHODS:The human pancreatic carcinoma cell line BxPC-3 was cultured in vitro.The growth inhibition rate,cell cycle and apoptotic rate of cells treated with 5-fluorouracil（5-FU）,sulfasalazine alone or a combination at different concentrations were evaluated with the MTT method and flow cytometry.Phase-contrast microscopy was used to observe morphological changes in the cells treated with 5-FU,sulfasalazine or both for 24 hours. RESULTS:The growth inhibition rate of the BxPC-3 cells treated with 5-FU and sulfasalazine significantly increased in a time-and dose-dependent manner.The growth inhibition rate of the cells treated with 5-FU gradually increased,but decreased at different concentrations of sulfasalazine for a prolonged period.The apoptotic rate of the BxPC-3 cells induced by sulfasalazine（200 mg/L）, 5-FU（100 mg/L）or both for 12 hours were（2.68±0.36）%, （6.59±0.90）%,and（10.52±0.55）%,respectively,compared with the corresponding control values were（3.17±0.08）%, （1.50±0.06）%,and（4.08±0.31）%[(t=2.33（P】0.05）,9.78 and 17.56（P【0.01）].It increased to（7.63±0.68）%,（40.43± 1.79）%,and（64.69±0.82）%for 48 hours,in comparison with the control that was（29.20±2.18）%,（5.61±0.13）%,and（12.02±0.52）%[t=17.06,33.66 and 94.51（P【0.01）]. The apoptotic rate,proportion of cells in S-phase and proliferative index rose after use of 5-FU（12.5,25,50,75, and 100 mg/L）alone for 24 hours.However,the apoptotic rate at augmented concentrations of sulfasalazine for 24 hours slowly increased from（1.47±0.08）%to（3.45± 0.28）%,the proportion of cells in G0/G1-phase increased from（35.13±0.32）%to（54.32±1.45）%,the proportion of cells in S-phase decreased from（45.37±1.48）%to（16.67± 2.73）%,and the proliferative index gradually lowered.The proportion of G0/G1-phase cells treated by 5-FU（100 mg/L） and sulfasalazine（200 mg/L）increased from（43.31±1.52）% （12 hours）to（85.05±0.24）%（48 hours）compared with the corresponding controls[t=7.93（12 hours）,21.30（48 hours）,P【0.01],and the proportion of cells in S-phase decreased from（11.63±1.11）%（12 hours）to（4.47±0.68）% （48 hours）in contrast to the controls[t=37.68（12 hours）, 8.60（48 hours）,P【0.01].Most cells after the combined use of the two agents for 24 hours displayed pyknosis and oval shape by phase-contrast microscopy.The cells treated with 5-FU（100 mg/L）for 24 hours were pyknotic and oval shaped.A few of cells in the group treated with sulfasalazine（200 mg/L）were pyknotic at 24 hours. CONCLUSIONS:Sulfasalazine may enhance the inhibitory proliferation and apoptosis effect on BxPC-3 cells induced by 5-FU,which is closely related to synergistically the cell cycle arrested in G0/G1-phase.

星基ADS-B系统空天链路的建模与仿真

星基广播式自动相关监视(ADS-B,automatic dependent surveillance-broadcast)系统是一种新型的航空器监视技术,在未来空中交通管理系统中具有广阔的应用前景。为了深入研究星基ADS-B系统中航空器到卫星的空天链路通信性能,将专业软件Matlab和STK(system tool kit)有效联合,构建符合国际标准的星基ADS-B空天链路完整模型;通过离散事件动态交互模拟ADS-B消息的发送与接收全过程,最后统计得出体现星基ADS-B空天链路通信性能的消息识别概率(POI,possibility of identify)、消息检测概率(POD,possibility of detective)、信号接收功率、信号冲突概率、卫星覆盖范围等指标。仿真结果表明,随着区域内航空器数量上升,POI、POD下降,消息冲突概率上升。

基于ADS-B的雷达测量误差分析方法

本文结合实际工程项目,介绍了广播式自动相关监视(ADS-B)地面接收数据的结构及后续存储、提取方法,同时介绍了雷达数据处理的流程,在此基础上对两路数据进行分析对比,对雷达测量误差进行分析,该方法操作简单,易于实现,且能够满足工程需求。

基于迁移学习-元学习的ADS-B攻击分类研究

准确分类通航领域ADS-B攻击类型,采取预防攻击措施,对保障通航运行安全性具有重要意义。针对通航领域ADS-B攻击数据样本少,提出一种基于迁移学习-元学习的ADS-B攻击分类模型。该模型将迁移学习与深度卷积自编码器相结合,建立ADS-B攻击特征提取模型,提取数据样本的攻击有效特征表示,并运用元学习策略在特征空间中实现ADS-B攻击准确分类。实例研究表明,基于迁移学习-元学习的攻击分类模型可有效分类小样本ADS-B攻击,且正确率在95%以上。

基于迁移学习-元学习的ADS-B攻击分类研究

准确分类通航领域ADS-B攻击类型,采取预防攻击措施,对保障通航运行安全性具有重要意义。针对通航领域ADS-B攻击数据样本少,提出一种基于迁移学习-元学习的ADS-B攻击分类模型。该模型将迁移学习与深度卷积自编码器相结合,建立ADS-B攻击特征提取模型,提取数据样本的攻击有效特征表示,并运用元学习策略在特征空间中实现ADS-B攻击准确分类。实例研究表明,基于迁移学习-元学习的攻击分类模型可有效分类小样本ADS-B攻击,且正确率在95%以上。

探究维生素ADE对母牛繁殖性能影响的研究

[目的]本试验旨在研究注射维生素ADE对母牛繁殖性能的影响。[方法]试验选取饲养管理条件相同、不同胎次、怀孕7个月的西门塔尔母牛共3批次,每批次试验组30头、对照组20头,3批次共计150头。对照组母牛严格执行保健护理程序,在产后14 d、28 d、42 d各肌注PGF_(2α)4 mL,从第42天注射PGF_(2α)的次日起,每天全天候视频监控发情情况,连续观察15 d。试验组母牛产前60 d、产前30 d、产后2 h内各肌肉分点注射维生素ADE注射液10 mL,其余程序均与对照组相同,从第42天肌肉注射PGF_(2α)的次日起,每天全天候视频监控发情情况,连续观察15 d。[结果]结果显示,试验组的胎衣不下的发病率显著低于对照组(P<0.05),产后瘫痪、子宫内膜炎的发病率极显著低于对照组(P<0.01),试验组的发情率极显著高于对照组(P<0.01),试验组的情期受胎率极显著高于对照组(P<0.01)。【结论】注射维生素ADE不仅能显著减少母牛产后胎衣不下、产后瘫痪、子宫内膜炎的发病率;还能显著提高产后母牛的发情率和情期受胎率。

基于多时间特征融合网络的ADS-B实采信号分离

不同于以往单天线广播式自动相关监视(Automatic Dependent Surveillance-Broadcast,ADS-B)信号分离中利用仿真的ADS-B信号制作数据集,将单天线接收的真实飞机发射的ADS-B原始信号通过调整信号起始时间以及功率并人为增加噪声来制作数据集。为了提高信号分离的时域波形精度,提出一种多分辨率多时间特征融合重采样(Multi-Temporal fusion Resampling of Multi-Resolution Features,MTRM-RF)网络,通过卷积将信号转化成不同采样率的信号并分别使用多层堆叠逐渐膨胀的一维卷积提取不同时间间隔的特征,以获得更多的时间信息。对多种基于深度学习的语音分离网络进行比较发现,MTRM-RF网络能够有效地融合ADS-B信号的不同采样率、不同时间间隔采样点的特征进行训练。并且随着训练集数据量的增加,分离信号的平均解码正确率达到88.39%,证明该网络可有效分离单天线实采的ADS-B交织信号。

基于AHP-AD-FEC的直线型倾转翼植保无人机设计研究

基于用户需求,系统性地对直线型倾转翼植保无人机进行优化设计。提出集成AHP层次分析法、AD公理设计和FEC模糊综合评价法的理论模型,指导设计实践。通过观察和访谈获取用户需求,使用AHP进行需求权重分析;运用AD理论将需求转化为技术参数并开展设计实践;最后,应用FEC对设计方案进行综合评价。运用该理论模型能够从满足用户需求的角度对直线型倾转翼植保无人机进行系统性优化设计。评价结果表明该设计流程有效,能够为同类产品设计提供新思路。

基于AD与TRIZ的加工中心主轴松拉刀机构优化设计

数控加工中心在加工过程中常出现主轴松刀力不足或无法松刀的现象。针对此问题,通过TRIZ系统组件分析明确现有松拉刀机构各组件间作用关系,利用因果链分析得出影响松刀的根本原因,最终应用TRIZ理论中的物场模型分析方法和AD独立性原理提出优化设计方案。根据新方案计算出实际工况下的主轴松刀力和螺杆强度,并对松刀杆进行建模和仿真。结果表明:最大位移量为6.995μm,最大应力为45.24 MPa,符合结构设计要求。最后,经过现场实物测试,当液压力为5 MPa时,松刀行程、松刀力以及打刀量均符合生产要求,验证了优化设计方案的合理性。

白菜型冬油菜BrADS2基因的克隆、表达分析与亚细胞定位

【目的】探究白菜型冬油菜(Brassica rapa L.)脂肪酸去饱和酶ADS2(Δ9 fatty acid desaturase)基因与抗寒性的关系。【方法】基于前期转录组结果,采用一步定向克隆法得到BrADS2基因;构建GFP融合表达载体,通过注射法在烟草下表皮细胞进行瞬时转化,同时采用农杆菌侵染法侵染‘中双11号’;利用qRT-PCR技术,检测抗寒性不同的白菜型冬油菜品种叶片中ADS2基因的表达模式,并对低温下冬油菜的形态变化和抗氧化酶活性、电导率含量进行分析。【结果】白菜型冬油菜BrADS2基因可编码307个氨基酸,该基因编码的蛋白具有亲水性特性;qRT-PCR结果显示,BrADS2基因的表达量呈先升后降的单峰型变化,且在-4℃时表达量达到峰值;亚细胞定位结果显示BrADS2蛋白在细胞质和细胞膜上表达;低温处理下3个品种叶片的相对电导率、抗氧化物酶活性均显著上升,强抗寒品种抗氧化酶活性高于弱抗寒品种,电导率却相反;农杆菌介导侵染‘中双11号’下胚轴后获得15株转基因阳性苗,且转基因油菜阳性植株叶中的BrADS2基因表达量均显著高于野生型。【结论】推测BrADS2基因在白菜型冬油菜膜脂不饱和调节中起重要作用,揭示了ADS2基因与冬油菜抗低温胁迫的关系。