Sustainable use of palm oil mill effluent (POME) has been the major focus in the recent development in palm oil industry due to the fact that environmental issue brought by POME. The purpose of this study was to deter...Sustainable use of palm oil mill effluent (POME) has been the major focus in the recent development in palm oil industry due to the fact that environmental issue brought by POME. The purpose of this study was to determine the optimum incubation period of purple non-sulphur bacterium (PNSB) in reduction of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) in settled POME and to determine the dry cell weight, TN, TP and cell yield of PNSB. Pure isolate of Rhodobacter sphaeroides strain UMSFW1 was cultured in settled POME under anaerobic condition at 2500 lux illumination on light intensity at a temperature of 30°C ± 2°C for 144-h. Parameters such as COD (mg/L), dry cell biomass (g/L), TP (mg/L) and TN (mg/L) in settled POME and bacterial cells were analyzed. A total reduction of TN (43.9%) in settled POME and a total increase of TN (43.2%) in bacterial cell were recorded at the end of experiment. At the same time the reduction of 51.5% chemical oxygen demand was determined from the POME. The highest dry cell weight of 2.44 g/L with cell yield 0.39 (mg/cell/mg COD) was achieved at the end of experiment. A total 24.7% of TP reduction in settled POME was achieved in 144-h culture, but while a maximum 10% of TP in bacterial cell was achieved in 48-h culture. This study shows that PNSB Rhodobacter sphaeroides strain UMSFW1 grows well by using settled POME as substrate and is capable to remove TN in the settled POME and assimilate into bacterial biomass. This study could provide us a further insight in the nutrient removal and COD removal in the bioremediation process by bacterium Rhodobacter sphaeroides strain UMSFW1.展开更多
已持续26年的湖南祁阳红壤站长期肥料试验分三个处理:NH4Cl+KCl+KH2PO4;尿素+KCl+过磷酸钙;(NH4)2SO4+K2SO4+过磷酸钙。其土壤、水稻植株样品被用来研究长期施用含硫化肥对水稻土酸化、SO42--S在土壤中累积、土壤-水稻系统微量元素含量...已持续26年的湖南祁阳红壤站长期肥料试验分三个处理:NH4Cl+KCl+KH2PO4;尿素+KCl+过磷酸钙;(NH4)2SO4+K2SO4+过磷酸钙。其土壤、水稻植株样品被用来研究长期施用含硫化肥对水稻土酸化、SO42--S在土壤中累积、土壤-水稻系统微量元素含量和水稻产量的影响。结果表明:大量(667 kg S hm-2 yr-1)施用含硫化肥15年后已导致水稻土表层土壤pH明显下降。不施硫处理SO42--S逐年下降,而施硫处理SO42--S有明显累积。施用含硫化肥24年后(1998),土壤有效Cu、B、Mn及全Ca含量有增加趋势,全Fe有减少趋势,对有效Mo、Zn及总Mg似乎没有影响。长期施用含硫化肥已显著减少水稻(特别是晚稻)吸收Fe、Mo、B、Mg、Cl,并导致水稻(特别是晚稻)产量显著下降。可见,长期施用含硫化肥可能影响水稻土性质进而影响水稻生长。展开更多
文摘Sustainable use of palm oil mill effluent (POME) has been the major focus in the recent development in palm oil industry due to the fact that environmental issue brought by POME. The purpose of this study was to determine the optimum incubation period of purple non-sulphur bacterium (PNSB) in reduction of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) in settled POME and to determine the dry cell weight, TN, TP and cell yield of PNSB. Pure isolate of Rhodobacter sphaeroides strain UMSFW1 was cultured in settled POME under anaerobic condition at 2500 lux illumination on light intensity at a temperature of 30°C ± 2°C for 144-h. Parameters such as COD (mg/L), dry cell biomass (g/L), TP (mg/L) and TN (mg/L) in settled POME and bacterial cells were analyzed. A total reduction of TN (43.9%) in settled POME and a total increase of TN (43.2%) in bacterial cell were recorded at the end of experiment. At the same time the reduction of 51.5% chemical oxygen demand was determined from the POME. The highest dry cell weight of 2.44 g/L with cell yield 0.39 (mg/cell/mg COD) was achieved at the end of experiment. A total 24.7% of TP reduction in settled POME was achieved in 144-h culture, but while a maximum 10% of TP in bacterial cell was achieved in 48-h culture. This study shows that PNSB Rhodobacter sphaeroides strain UMSFW1 grows well by using settled POME as substrate and is capable to remove TN in the settled POME and assimilate into bacterial biomass. This study could provide us a further insight in the nutrient removal and COD removal in the bioremediation process by bacterium Rhodobacter sphaeroides strain UMSFW1.
文摘已持续26年的湖南祁阳红壤站长期肥料试验分三个处理:NH4Cl+KCl+KH2PO4;尿素+KCl+过磷酸钙;(NH4)2SO4+K2SO4+过磷酸钙。其土壤、水稻植株样品被用来研究长期施用含硫化肥对水稻土酸化、SO42--S在土壤中累积、土壤-水稻系统微量元素含量和水稻产量的影响。结果表明:大量(667 kg S hm-2 yr-1)施用含硫化肥15年后已导致水稻土表层土壤pH明显下降。不施硫处理SO42--S逐年下降,而施硫处理SO42--S有明显累积。施用含硫化肥24年后(1998),土壤有效Cu、B、Mn及全Ca含量有增加趋势,全Fe有减少趋势,对有效Mo、Zn及总Mg似乎没有影响。长期施用含硫化肥已显著减少水稻(特别是晚稻)吸收Fe、Mo、B、Mg、Cl,并导致水稻(特别是晚稻)产量显著下降。可见,长期施用含硫化肥可能影响水稻土性质进而影响水稻生长。
