[ Objective ] The paper was to study the effect of controlled atmosphere stress of carbon dioxide （ CO2 ） on Superoxide dismutase （SOD） activity of in- sect, so as to analyze the biological mechanism of the action...[ Objective ] The paper was to study the effect of controlled atmosphere stress of carbon dioxide （ CO2 ） on Superoxide dismutase （SOD） activity of in- sect, so as to analyze the biological mechanism of the action of controlled atmosphere stress on insect. [ Method] Using nitrotetrazolium blue chloride （NBT） light reduction method, SOD activity of drugstore beetle （ Stegobium panlceum ）, cigarette beetle （ Lasioderma serricorne） and coffee bean beetle （Araecerus fasciculatus） was studied, and the stress response of the enzyme under controlled atmosphere stress of CO2 was analyzed. [ Result ] SOD activity of drugstore beetle, cigarette beetle and coffee bean beetle exposed to controlled atmosphere stress of high concentrations of CO2 for 3 and 6 h had certain degree of increase, and the activity sig- nificantly increased from 2.011±0.954,2.664±0.218 and 1.458±0.718 to 3. 135±0. 105,3.050±0.673 and 2.975±0.229 U/（per pest · 30 min） after treat- ment for 6 h. [ Conclusion] Controlled atmosphere stress of high concentrations of CO2 had certain activation effect on SOD activity of storage pest in Chinese me- dicinal material within the context of sub-lethal events. The results could enrich the insecticidal mechanism of controlled atmosphere and theoretical system of analy- sis on insect resistance to controlled atmosphere.展开更多
Objective: To probe into the mechanism of moxibustion preconditioning in preventive brain-protecting effect. Methods: The global brain ischemia rat model was developed by blocking 4 arteries. Seventy-eight Wistar male...Objective: To probe into the mechanism of moxibustion preconditioning in preventive brain-protecting effect. Methods: The global brain ischemia rat model was developed by blocking 4 arteries. Seventy-eight Wistar male rats were randomly divided into 5 groups: a normal control group, a sham-operation group, a brain ischemia group, a brain ischemia preconditioning group, a moxibustion pretreating group. The brains in the 5 groups were taken at 24 h, 48 h, and 72 h after operation respectively. Superoxide dismulase (SOD) activity was determined with xanthine oxidase method and malondialdehyde (MDA) content with thiobarbituric acid method. Results: After the operation, in the moxibustion preconditioning group, SOD activity significantly increased, especially 24 h after moxibustion preconditioning; and MDA content decreased, with a very significant difference as compared with that of the cerebral ischemia group (P<0.01). Conclusion: Moxibustion preconditioning protects the ischemic and anoxic brain tissue by increasing the activity of endogenous antioxidase.展开更多
基金Supported by Project of Guizhou Provincial Modernization of Chinese Medicine Material Project(QKHYZ[2011]5049Organization Department of CPCGuizhou Committee(TZJF-2009-02)Promotion Project of Key Scientific and Technological Achievements of Guiyang City([2010]1-T-4)~~
文摘[ Objective ] The paper was to study the effect of controlled atmosphere stress of carbon dioxide （ CO2 ） on Superoxide dismutase （SOD） activity of in- sect, so as to analyze the biological mechanism of the action of controlled atmosphere stress on insect. [ Method] Using nitrotetrazolium blue chloride （NBT） light reduction method, SOD activity of drugstore beetle （ Stegobium panlceum ）, cigarette beetle （ Lasioderma serricorne） and coffee bean beetle （Araecerus fasciculatus） was studied, and the stress response of the enzyme under controlled atmosphere stress of CO2 was analyzed. [ Result ] SOD activity of drugstore beetle, cigarette beetle and coffee bean beetle exposed to controlled atmosphere stress of high concentrations of CO2 for 3 and 6 h had certain degree of increase, and the activity sig- nificantly increased from 2.011±0.954,2.664±0.218 and 1.458±0.718 to 3. 135±0. 105,3.050±0.673 and 2.975±0.229 U/（per pest · 30 min） after treat- ment for 6 h. [ Conclusion] Controlled atmosphere stress of high concentrations of CO2 had certain activation effect on SOD activity of storage pest in Chinese me- dicinal material within the context of sub-lethal events. The results could enrich the insecticidal mechanism of controlled atmosphere and theoretical system of analy- sis on insect resistance to controlled atmosphere.
文摘Objective: To probe into the mechanism of moxibustion preconditioning in preventive brain-protecting effect. Methods: The global brain ischemia rat model was developed by blocking 4 arteries. Seventy-eight Wistar male rats were randomly divided into 5 groups: a normal control group, a sham-operation group, a brain ischemia group, a brain ischemia preconditioning group, a moxibustion pretreating group. The brains in the 5 groups were taken at 24 h, 48 h, and 72 h after operation respectively. Superoxide dismulase (SOD) activity was determined with xanthine oxidase method and malondialdehyde (MDA) content with thiobarbituric acid method. Results: After the operation, in the moxibustion preconditioning group, SOD activity significantly increased, especially 24 h after moxibustion preconditioning; and MDA content decreased, with a very significant difference as compared with that of the cerebral ischemia group (P<0.01). Conclusion: Moxibustion preconditioning protects the ischemic and anoxic brain tissue by increasing the activity of endogenous antioxidase.
