Half of altogether 60 cylindrical implant devices made of titanium-aluminum-vanadium alloy （ Ti-6Al-4V） were plusna-sprayed with a hydroxyapatite-couting and the other half had a corundum blasted porous surface. 15...Half of altogether 60 cylindrical implant devices made of titanium-aluminum-vanadium alloy （ Ti-6Al-4V） were plusna-sprayed with a hydroxyapatite-couting and the other half had a corundum blasted porous surface. 15 implants of each group of the titanium test buplants were coated with 230 μg porcine, high-purified BMP- 3-precipitute per implant. In each case a BMP- 3-couted and an uncoated control-device were implanted into the femoral part of the putellofemoral joint of the right and left leg of 30 adult giant rabbits. Histomorphological and histomorphometrical we found in both groups with BMP- 3-coated test devices an improved osteointegrution. Stutistical evaluation using the t-test for matched samples showed 5 weeks after surgery a significant higher volume of tony formed bone of the BMP- 3-coated corundum- blasted or hydroxyapathe- coated Ti- 6Al- 4 V test devices compared to the non-couted controls of the same t）pe （p 〈 0.01, t-test for matched samples）. In both implant groups with BMP-couting a synergetic effect was verifiable although the bone ongrowth in the hydroxyaputite coated implants was more extensive than in the corundum blasted implants. Light microscopy demonstrated osteointegrution without connective tissue membrane around the surface of the implants. Our results indicate that composite metal implants,as used in endoprosthetics and implantology , are suitable carriers for BMP- 3 and im proved fixation of the implants can be achieved. The hydroxyapatite surface is superior to the corundum-blasted surface with regards to the observed parameters because of its pronounced bioactivity and its osteoconductive characteristics.展开更多
The morphology of bone repair materials,such as particle size and roughness of the materials surface,can affect the adsorption of protein molecules.The effects of surface morphology on the absorption of proteins with ...The morphology of bone repair materials,such as particle size and roughness of the materials surface,can affect the adsorption of protein molecules.The effects of surface morphology on the absorption of proteins with different charges were studied.Submicron and nano hydroxyapatite(HA)powders prepared by the chemical precipitation method were coated on the surface of a gold sheet by electrophoretic deposition.Various hydroxyapatite coating morphologies were obtained by controlling the powder particle size and the deposition time.The coating surface morphology was analyzed by Atomic Force Microscopy(AFM),and the adsorption behavior of differently charged proteins on the surface was dynamically monitored by Quartz Crystal Microbalance with Dissipation(QCM-D).The adsorption dependence of two proteins with different charges upon hydroxyapatite coating surface morphology was investigated.Results show that coating surfaces with smaller deposited particle sizes are favorable for the adsorption of negatively-charged bovine albumin,while with larger deposited particles facilitate the adsorption of positively-charged lysozyme.This may be because that the negatively-charged hydroxyapatite coating exhibits stronger electrostatic effect as the increase in the coating particle size,which facilitates the adsorption of positively-charged proteins and hinders the adsorption of negatively-charged proteins.Increasing coating surface roughness facilitates protein adsorption,though the particle size exhibits a dominant influence.These results are significant for selective adsorption of proteins on material surfaces.展开更多
文摘Half of altogether 60 cylindrical implant devices made of titanium-aluminum-vanadium alloy （ Ti-6Al-4V） were plusna-sprayed with a hydroxyapatite-couting and the other half had a corundum blasted porous surface. 15 implants of each group of the titanium test buplants were coated with 230 μg porcine, high-purified BMP- 3-precipitute per implant. In each case a BMP- 3-couted and an uncoated control-device were implanted into the femoral part of the putellofemoral joint of the right and left leg of 30 adult giant rabbits. Histomorphological and histomorphometrical we found in both groups with BMP- 3-coated test devices an improved osteointegrution. Stutistical evaluation using the t-test for matched samples showed 5 weeks after surgery a significant higher volume of tony formed bone of the BMP- 3-coated corundum- blasted or hydroxyapathe- coated Ti- 6Al- 4 V test devices compared to the non-couted controls of the same t）pe （p 〈 0.01, t-test for matched samples）. In both implant groups with BMP-couting a synergetic effect was verifiable although the bone ongrowth in the hydroxyaputite coated implants was more extensive than in the corundum blasted implants. Light microscopy demonstrated osteointegrution without connective tissue membrane around the surface of the implants. Our results indicate that composite metal implants,as used in endoprosthetics and implantology , are suitable carriers for BMP- 3 and im proved fixation of the implants can be achieved. The hydroxyapatite surface is superior to the corundum-blasted surface with regards to the observed parameters because of its pronounced bioactivity and its osteoconductive characteristics.
基金This work was financially supported by the National Natural Science Foundation of China(Grant Nos.51972120 and 51772105).
文摘The morphology of bone repair materials,such as particle size and roughness of the materials surface,can affect the adsorption of protein molecules.The effects of surface morphology on the absorption of proteins with different charges were studied.Submicron and nano hydroxyapatite(HA)powders prepared by the chemical precipitation method were coated on the surface of a gold sheet by electrophoretic deposition.Various hydroxyapatite coating morphologies were obtained by controlling the powder particle size and the deposition time.The coating surface morphology was analyzed by Atomic Force Microscopy(AFM),and the adsorption behavior of differently charged proteins on the surface was dynamically monitored by Quartz Crystal Microbalance with Dissipation(QCM-D).The adsorption dependence of two proteins with different charges upon hydroxyapatite coating surface morphology was investigated.Results show that coating surfaces with smaller deposited particle sizes are favorable for the adsorption of negatively-charged bovine albumin,while with larger deposited particles facilitate the adsorption of positively-charged lysozyme.This may be because that the negatively-charged hydroxyapatite coating exhibits stronger electrostatic effect as the increase in the coating particle size,which facilitates the adsorption of positively-charged proteins and hinders the adsorption of negatively-charged proteins.Increasing coating surface roughness facilitates protein adsorption,though the particle size exhibits a dominant influence.These results are significant for selective adsorption of proteins on material surfaces.
