Vibrio harveyi is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry.Recurrent outbreaks of vibriosis are closely correlated with the ...Vibrio harveyi is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry.Recurrent outbreaks of vibriosis are closely correlated with the capacity of this bacterial species to survive long-term starvation conditions.To study the regulation mechanism of gene expression at the transcriptional level in V.harveyi under starvation conditions,the transcriptomic response profiles were determined of the Portunus trituberculatus pathogen V.harveyi strain DY1 under normal conditions and after four weeks of starvation.A total of 4679 and 4661 genes were expressed in the non-starved and starved cells,respectively.The significantly differentially expressed genes(DEGs)between non-starved and starved groups were identified,in which 255 genes were up-regulated and 411 genes were down-regulated.GO analysis and KEGG enrichment analysis were used to analyze the DEGs and revealed the involvement of these DEGs in many pathways,including ABC transporters,flagellum assembly,and fatty acid metabolism.Several DEGs were randomly selected and their expression levels were confirmed by quantitative real-time PCR(qRT-PCR).This is the first comprehensive transcriptomic analysis of starvation ef fects in V.harveyi.Our findings will facilitate future study on stress adaptation and survival mechanisms of V.harveyi.展开更多
In this paper, we propose a new backup-VP priority restoration method for ATM survival net-works. in order to improve the survivability performance of higher priority VPs in case of multi-failure under the minim...In this paper, we propose a new backup-VP priority restoration method for ATM survival net-works. in order to improve the survivability performance of higher priority VPs in case of multi-failure under the minimum amout of spare capacity required. This method is a part of a hybrid restoration ,mechanism (to be presented later), which integrates a backup-VP, method￣[2] and a distributed flooding algorithm￣[3] in theATM layer. This method is a path restoration between VP terminal nodes, completes the restoration with bi-directional message transmission by ustng pre-assigned backup VP's in case of single failure, and improvesthe restoration time of higher priority VPs in case of multi-failure, but it should cooperate with anotherorganic part of the hybrid restoration mechanism-a floodting algorithm to restore the remaining lower priorityVPs. This method can use resource more efficiently and obtain a better cost-performance feature.展开更多
基金Supported by the Jiangsu Agricultural Industry Technology System(No.02)the National Natural Science Foundation of China(No.31972830)+3 种基金the Natural Science Foundation of Jiangsu Province(No.BK20180915)the Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(18)2012)the Fishery Science and Technology Innovation Projects of Jiangsu Province(No.D2017-3)the “Blue Project” of Yangzhou University and the Projects of Shuichan Sanxin of Jiangsu Province(No.Y2016-33,D2017-3)
文摘Vibrio harveyi is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry.Recurrent outbreaks of vibriosis are closely correlated with the capacity of this bacterial species to survive long-term starvation conditions.To study the regulation mechanism of gene expression at the transcriptional level in V.harveyi under starvation conditions,the transcriptomic response profiles were determined of the Portunus trituberculatus pathogen V.harveyi strain DY1 under normal conditions and after four weeks of starvation.A total of 4679 and 4661 genes were expressed in the non-starved and starved cells,respectively.The significantly differentially expressed genes(DEGs)between non-starved and starved groups were identified,in which 255 genes were up-regulated and 411 genes were down-regulated.GO analysis and KEGG enrichment analysis were used to analyze the DEGs and revealed the involvement of these DEGs in many pathways,including ABC transporters,flagellum assembly,and fatty acid metabolism.Several DEGs were randomly selected and their expression levels were confirmed by quantitative real-time PCR(qRT-PCR).This is the first comprehensive transcriptomic analysis of starvation ef fects in V.harveyi.Our findings will facilitate future study on stress adaptation and survival mechanisms of V.harveyi.
文摘In this paper, we propose a new backup-VP priority restoration method for ATM survival net-works. in order to improve the survivability performance of higher priority VPs in case of multi-failure under the minimum amout of spare capacity required. This method is a part of a hybrid restoration ,mechanism (to be presented later), which integrates a backup-VP, method￣[2] and a distributed flooding algorithm￣[3] in theATM layer. This method is a path restoration between VP terminal nodes, completes the restoration with bi-directional message transmission by ustng pre-assigned backup VP's in case of single failure, and improvesthe restoration time of higher priority VPs in case of multi-failure, but it should cooperate with anotherorganic part of the hybrid restoration mechanism-a floodting algorithm to restore the remaining lower priorityVPs. This method can use resource more efficiently and obtain a better cost-performance feature.
