Aim： To test the hypothesis that season affects the semen quality of swamp buffalo （Bubalus bubalis） bulls used for artificial insemination （AI） under tropical conditions in Thailand, as it does in Bos taurus and...Aim： To test the hypothesis that season affects the semen quality of swamp buffalo （Bubalus bubalis） bulls used for artificial insemination （AI） under tropical conditions in Thailand, as it does in Bos taurus and Bos indicus. Methods： Clinical and andrological examinations, and monitoring of semen production and quality were carried out on five mature, healthy swamp buffalo AI bulls in Thailand from July 2004 to the end of June 2005. Sperm output, motility, morphology and plasma membrane integrity （PMI） were compared between three seasons of the year （rainy, i.e. July-October; winter, i.e. November-February; and summer, i.e. March-June） with distinct ambient temperature and humidity. Results： All bulls were diagnosed as clinically healthy and with good libido throughout the study. Ejaculate volume, pH, sperm concentration, total sperm number and initial sperm motility did not differ between seasons, whereas PMI and the relative proportion of morphologically normal spermatozoa were highest in summer and lowest in winter （P 〈 0.05）. Buffalo age, week of collection and season influenced sperm morphology （P 〈 0.05-0.001）. Among morphological abnormalities, only proportions of tail defects were affected by season, being highest in the rainy season and lowest in summer （P 〈 0.001）. In conclusion, climatic changes did not seem to largely affect semen sperm output or viability. Although the proportions of PMI and tail abnormalities were affected by season, they were always below what is considered unacceptable for AI bull sires. Conclusion： Seasonal changes did not appear to cause deleterious changes in sperm quality in swamp buffalo AI-sires in tropical Thailand.展开更多
[ Objective] To locate gonadotropin-releasing hormone （GnRH） in pituitary of Guangxi swamp buffaloes and to provide a theoretical ba- sis for cloning and sequence analysis of GnRH receptor gene. [ Method] GnRH in pi...[ Objective] To locate gonadotropin-releasing hormone （GnRH） in pituitary of Guangxi swamp buffaloes and to provide a theoretical ba- sis for cloning and sequence analysis of GnRH receptor gene. [ Method] GnRH in pituitary were immunohistochemically stained by avidin biotin complex method. The GnRH expression was analyzed with image system. The GnRH receptor gene was amplified by real-time PCR. [ Result] Many GnRH positive cells were detected in pars distalis of adenohypophysis. GnRH were distributed in cytoplasm but not in nuclei. No positive sig- nal was observed in neurohypophysis. In addition, the GnRH receptor gene, 920 bp in size, was amplified. [ Conclusion] A large number of GnRH and GnRH receptor were found in pars distalis of adenohypophysis, which indicates that anterior pituitary is an important tissue for functions of hypo- thalamus-pituitary-gonadal axis and other endocrine axes.展开更多
[Objective] The aim of this paper is to find the change law of reproductive hormones of female swamp buffalo in off season for estrus and to investigate the relationship between various hormones. [ Method] The concent...[Objective] The aim of this paper is to find the change law of reproductive hormones of female swamp buffalo in off season for estrus and to investigate the relationship between various hormones. [ Method] The concentrations of follicle stimulating hormone （FSH）, luteinizing hor- mone （ LH）, inhibin （ INH）, estradiol （ E2 ） and progesterone （ P4 ） were determined by the enzyme-linked immunosorbent assay （ELISA） in off season for estrue. The changes of follicles in the estrous cycle were examined using B-mode ultrasonography. [ Result] In off season for estrus of female swamp buffalo from Guangxi region, the blood concentration of E2 and P4 varied fluctuantly. The concentration of E2 had two peaks, respec- tively 3 d before ovulation and after 13 d post ovulation. The concentration of P4 increased slowly from the first day after ovulation, increased rapidly from 5 to 13 d, peaked on Day 16 and decreased to the baseline 3 d before ovulation. The concentrations of FSH, LH and INH roached a peak re- spectively after 10 d post ovulation, 2 d before ovulation, and after 4 d post ovulation. The changes of the hormones had correlations. The concen- trations of FSH, LH and INH had highly significant correlations with each other, while the concentration of INH had highly significant correlations with that of E2 or P4- The concentration of E2 also had a highly significant correlation with that of P4- [ Conclusionl The study can guide the breeding of swamp buffalo from Guanqxi reqion and also help to improve their roproduction performance.展开更多
12头健康禁食公水牛分为3组。组Ⅰ(n=6)与组Ⅱ(n=3)分别经颈静脉快速注射50%葡萄糖和0.9%生理盐水各2 mL.kg-1;组Ⅲ(n=3)为对照,不作处理。分别于注射前60、10 m in及注射后1、5、10、30、60、120、180、240、300、360和420 m in采血,...12头健康禁食公水牛分为3组。组Ⅰ(n=6)与组Ⅱ(n=3)分别经颈静脉快速注射50%葡萄糖和0.9%生理盐水各2 mL.kg-1;组Ⅲ(n=3)为对照,不作处理。分别于注射前60、10 m in及注射后1、5、10、30、60、120、180、240、300、360和420 m in采血,立即测定血浆葡萄糖及胰岛素和胰高血糖素,分析各指标间的相关性及血浆胰岛素/血糖值(IGR1)和胰岛素/胰高血糖素值(IGR2)。结果显示:注射前,各组水牛血浆葡萄糖、胰岛素和胰高血糖素的浓度接近。组Ⅱ和组Ⅲ水牛的上述指标在所有采样时间点上组内和组间均未表现出显著性差异(P>0.05),IGR1和IGR2也没有明显变化。组Ⅰ水牛静注葡萄糖1 m in后血糖浓度迅速升至峰值,而血浆胰岛素30 m in后才升高(约20倍)至峰值,然后两者均缓慢下降,直到420 m in仍明显高于注射前的水平(P<0.05)。而胰高血糖素仅在注射葡萄糖后1和5 m in时显示出差异(P<0.05),IGR1在注射葡萄糖(组Ⅰ)后立即下降,至注射后180 m in,组Ⅰ的IGR1和IGR2与组Ⅱ和组Ⅲ的基本持平。相关性分析表明,血糖浓度与血浆胰岛素水平呈极显著正相关(r=0.73,P<0.01),与胰高血糖素浓度呈显著负相关(r=-0.58,P<0.05),但胰岛素水平与胰高血糖素浓度相关性不显著(r=0.06,P>0.05)。提示:水牛胰岛素代谢迟缓,对静注的葡萄糖清除缓慢,表现出持续性高血糖症和高胰岛素血症;水牛对于静注葡萄糖的反应不同于奶牛、犬、马等其他动物。展开更多
文摘Aim： To test the hypothesis that season affects the semen quality of swamp buffalo （Bubalus bubalis） bulls used for artificial insemination （AI） under tropical conditions in Thailand, as it does in Bos taurus and Bos indicus. Methods： Clinical and andrological examinations, and monitoring of semen production and quality were carried out on five mature, healthy swamp buffalo AI bulls in Thailand from July 2004 to the end of June 2005. Sperm output, motility, morphology and plasma membrane integrity （PMI） were compared between three seasons of the year （rainy, i.e. July-October; winter, i.e. November-February; and summer, i.e. March-June） with distinct ambient temperature and humidity. Results： All bulls were diagnosed as clinically healthy and with good libido throughout the study. Ejaculate volume, pH, sperm concentration, total sperm number and initial sperm motility did not differ between seasons, whereas PMI and the relative proportion of morphologically normal spermatozoa were highest in summer and lowest in winter （P 〈 0.05）. Buffalo age, week of collection and season influenced sperm morphology （P 〈 0.05-0.001）. Among morphological abnormalities, only proportions of tail defects were affected by season, being highest in the rainy season and lowest in summer （P 〈 0.001）. In conclusion, climatic changes did not seem to largely affect semen sperm output or viability. Although the proportions of PMI and tail abnormalities were affected by season, they were always below what is considered unacceptable for AI bull sires. Conclusion： Seasonal changes did not appear to cause deleterious changes in sperm quality in swamp buffalo AI-sires in tropical Thailand.
基金supported by the grants from Research and Innovation Project for Master Degree Candidates(105930903014)Guangxi Natural Science Foundation(06400150832043 and 0991042+3 种基金Guangxi Science and Technology Department)Research Foundation of Guangxi Education Department(200709LX075Guangxi Education and Research)Guangxi Large-scale Instrument Collaboration and Sharing Network Program
文摘[ Objective] To locate gonadotropin-releasing hormone （GnRH） in pituitary of Guangxi swamp buffaloes and to provide a theoretical ba- sis for cloning and sequence analysis of GnRH receptor gene. [ Method] GnRH in pituitary were immunohistochemically stained by avidin biotin complex method. The GnRH expression was analyzed with image system. The GnRH receptor gene was amplified by real-time PCR. [ Result] Many GnRH positive cells were detected in pars distalis of adenohypophysis. GnRH were distributed in cytoplasm but not in nuclei. No positive sig- nal was observed in neurohypophysis. In addition, the GnRH receptor gene, 920 bp in size, was amplified. [ Conclusion] A large number of GnRH and GnRH receptor were found in pars distalis of adenohypophysis, which indicates that anterior pituitary is an important tissue for functions of hypo- thalamus-pituitary-gonadal axis and other endocrine axes.
基金funded by the China Natural Science Foundation(30760171)
文摘[Objective] The aim of this paper is to find the change law of reproductive hormones of female swamp buffalo in off season for estrus and to investigate the relationship between various hormones. [ Method] The concentrations of follicle stimulating hormone （FSH）, luteinizing hor- mone （ LH）, inhibin （ INH）, estradiol （ E2 ） and progesterone （ P4 ） were determined by the enzyme-linked immunosorbent assay （ELISA） in off season for estrue. The changes of follicles in the estrous cycle were examined using B-mode ultrasonography. [ Result] In off season for estrus of female swamp buffalo from Guangxi region, the blood concentration of E2 and P4 varied fluctuantly. The concentration of E2 had two peaks, respec- tively 3 d before ovulation and after 13 d post ovulation. The concentration of P4 increased slowly from the first day after ovulation, increased rapidly from 5 to 13 d, peaked on Day 16 and decreased to the baseline 3 d before ovulation. The concentrations of FSH, LH and INH roached a peak re- spectively after 10 d post ovulation, 2 d before ovulation, and after 4 d post ovulation. The changes of the hormones had correlations. The concen- trations of FSH, LH and INH had highly significant correlations with each other, while the concentration of INH had highly significant correlations with that of E2 or P4- The concentration of E2 also had a highly significant correlation with that of P4- [ Conclusionl The study can guide the breeding of swamp buffalo from Guanqxi reqion and also help to improve their roproduction performance.
文摘12头健康禁食公水牛分为3组。组Ⅰ(n=6)与组Ⅱ(n=3)分别经颈静脉快速注射50%葡萄糖和0.9%生理盐水各2 mL.kg-1;组Ⅲ(n=3)为对照,不作处理。分别于注射前60、10 m in及注射后1、5、10、30、60、120、180、240、300、360和420 m in采血,立即测定血浆葡萄糖及胰岛素和胰高血糖素,分析各指标间的相关性及血浆胰岛素/血糖值(IGR1)和胰岛素/胰高血糖素值(IGR2)。结果显示:注射前,各组水牛血浆葡萄糖、胰岛素和胰高血糖素的浓度接近。组Ⅱ和组Ⅲ水牛的上述指标在所有采样时间点上组内和组间均未表现出显著性差异(P>0.05),IGR1和IGR2也没有明显变化。组Ⅰ水牛静注葡萄糖1 m in后血糖浓度迅速升至峰值,而血浆胰岛素30 m in后才升高(约20倍)至峰值,然后两者均缓慢下降,直到420 m in仍明显高于注射前的水平(P<0.05)。而胰高血糖素仅在注射葡萄糖后1和5 m in时显示出差异(P<0.05),IGR1在注射葡萄糖(组Ⅰ)后立即下降,至注射后180 m in,组Ⅰ的IGR1和IGR2与组Ⅱ和组Ⅲ的基本持平。相关性分析表明,血糖浓度与血浆胰岛素水平呈极显著正相关(r=0.73,P<0.01),与胰高血糖素浓度呈显著负相关(r=-0.58,P<0.05),但胰岛素水平与胰高血糖素浓度相关性不显著(r=0.06,P>0.05)。提示:水牛胰岛素代谢迟缓,对静注的葡萄糖清除缓慢,表现出持续性高血糖症和高胰岛素血症;水牛对于静注葡萄糖的反应不同于奶牛、犬、马等其他动物。
