With the support by the National Natural Science Foundation of China,the research team led by Professor Li Yigang(李毅刚)at Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine and Professor ...With the support by the National Natural Science Foundation of China,the research team led by Professor Li Yigang(李毅刚)at Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine and Professor Peng Huisheng(彭慧胜)at Fudan University presented a new type of展开更多
Cell-free expression system is a technology for the synthesis of proteins in vitro.The system is a platform for several bioengineering projects,e.g.cell-free metabolic engineering,evolutionary design of experiments,an...Cell-free expression system is a technology for the synthesis of proteins in vitro.The system is a platform for several bioengineering projects,e.g.cell-free metabolic engineering,evolutionary design of experiments,and synthetic minimal cell construction.Bacterial cell-free protein synthesis system(CFPS)is a robust tool for synthetic biology.The bacteria lysate,the DNA,and the energy module,which are the three optimized sub-systems for in vitro protein synthesis,compose the integrated system.Currently,an optimized E.coli cell-free expression system can produce up to^2.3 mg/mL of a fluorescent reporter protein.Herein,I will describe the features of ATP-regeneration systems for in vitro protein synthesis,and I will present a machine-learning experiment for optimizing the protein yield of E.coli cell-free protein synthesis systems.Moreover,I will introduce experiments on the synthesis of a minimal cell using liposomes as dynamic containers,and E.coli cell-free expression system as biochemical platform for metabolism and gene expression.CFPS can be further integrated with other technologies for novel applications in environmental,medical and material science.展开更多
Coacervates formed by liquid-liquid phase separation play significant roles in a variety of intracellular and extracellular biological processes.Recently,substantial efforts have been invested in creating protocells u...Coacervates formed by liquid-liquid phase separation play significant roles in a variety of intracellular and extracellular biological processes.Recently,substantial efforts have been invested in creating protocells using coacervates.Microfluidic technology has rapidly gained prominence in this area due to its capability to construct monodisperse and stable coacervate droplets.This review highlights recent advancements in utilizing microfluidic devices to construct coacervate-core-vesicle(COV)systems.These COV systems can be employed to realize the sequestration and release of biomolecules as well as to control enzymatic reactions within the coacervate systems in a spatiotemporal manner.Lastly,we delve into the current challenges and opportunities related to the development of functional coacervate systems based on microfluidic technology.展开更多
文摘With the support by the National Natural Science Foundation of China,the research team led by Professor Li Yigang(李毅刚)at Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine and Professor Peng Huisheng(彭慧胜)at Fudan University presented a new type of
文摘Cell-free expression system is a technology for the synthesis of proteins in vitro.The system is a platform for several bioengineering projects,e.g.cell-free metabolic engineering,evolutionary design of experiments,and synthetic minimal cell construction.Bacterial cell-free protein synthesis system(CFPS)is a robust tool for synthetic biology.The bacteria lysate,the DNA,and the energy module,which are the three optimized sub-systems for in vitro protein synthesis,compose the integrated system.Currently,an optimized E.coli cell-free expression system can produce up to^2.3 mg/mL of a fluorescent reporter protein.Herein,I will describe the features of ATP-regeneration systems for in vitro protein synthesis,and I will present a machine-learning experiment for optimizing the protein yield of E.coli cell-free protein synthesis systems.Moreover,I will introduce experiments on the synthesis of a minimal cell using liposomes as dynamic containers,and E.coli cell-free expression system as biochemical platform for metabolism and gene expression.CFPS can be further integrated with other technologies for novel applications in environmental,medical and material science.
基金Grant,Grant/Award Number:MOET2EP30220-0006National Research Foundation Singapore,Grant/Award Number:NRF-NRFF11-2019-0004。
文摘Coacervates formed by liquid-liquid phase separation play significant roles in a variety of intracellular and extracellular biological processes.Recently,substantial efforts have been invested in creating protocells using coacervates.Microfluidic technology has rapidly gained prominence in this area due to its capability to construct monodisperse and stable coacervate droplets.This review highlights recent advancements in utilizing microfluidic devices to construct coacervate-core-vesicle(COV)systems.These COV systems can be employed to realize the sequestration and release of biomolecules as well as to control enzymatic reactions within the coacervate systems in a spatiotemporal manner.Lastly,we delve into the current challenges and opportunities related to the development of functional coacervate systems based on microfluidic technology.
