Ovarian follicle growth in three dimensional (3D) matrices in vitro has limitations: a) matrices don’t expand as follicles grow, b) requirements for enzyme-mediated retrieval, and c) animal-derived components prevent...Ovarian follicle growth in three dimensional (3D) matrices in vitro has limitations: a) matrices don’t expand as follicles grow, b) requirements for enzyme-mediated retrieval, and c) animal-derived components prevent clinical application. Therefore, we evaluated N-Isopropylacrylamide (SFX-1), a novel synthetic 3D culture matrix, for follicle culture. Groups of three murine secondary follicles were encapsulated in 50 μL of DMEM/F12-1%ITS-10%FCS (DMEM/F12) or SFX-1 (3:2 v/v DMEM/F12) or Matrigel (1:1 DMEM/F12) and cultured for 48 h. Matrigel contains growth factors but SFX-1 has no animal-derived factors. Each culture condition was examined in 6 wells containing 18 follicles, in four replicate experiments (n = 4). Photomicrographs were used to determine follicle diameters and morphological integrity. Follicles were Live-Dead (LD) stained or disaggregated to generate cells for viability assessment using Trypan Blue (TB). Estradiol, progesterone and anti-mullerian hormone (AMH) in conditioned media were measured using Enzyme-linked Immunoassay. All culture conditions supported similar increases in follicle diameter. DMEM/F12 did not maintain morphological integrity which prevented follicle retrieval after 48 h;25% were retrieved from DMEM/F12, but 44% and 41% follicles were retrieved from SFX-1 and Matrigel respectively. Follicles retrieved from Matrigel could not be disaggregated, which prevented TB viability assessment. LD estimations of viable cells/follicle were lower than TB, but culture conditions had no effect on viability;SFX-1 64% ± 8% and DMEM/F12 69% ± 9%. SFX-1 and Matrigel supported similar levels of progesterone synthesis, only Matrigel supported estrogen synthesis, but none of the culture conditions supported AMH production. SFX-1 was not cytotoxic and was comparable to Matrigel. Further development of SFX-1 for use with human follicles is supported.展开更多
On-line measurement for dielectric loss angle can effectively monitor the insulation condition of capacitive equipment in power systems. Synthetic relative measuring methods not only markedly overcome the shortcomings...On-line measurement for dielectric loss angle can effectively monitor the insulation condition of capacitive equipment in power systems. Synthetic relative measuring methods not only markedly overcome the shortcomings of traditional absolute measuring methods but also greatly improve the accuracy of dielectric loss angle measurement. However, synthetic relative measuring methods based on two or three pieces of capacitive equipment do not have the characteristic of generality. In this paper, a principle of synthetic relative measuring method is presented. The example of application for synthetic relative methods based on three and four pieces of capacitive equipment running in the same phase is taken to present the failure judgment matrices for N pieces of equipment. According to these matrices, the fault condition of N pieces of capacitive equipment can be watched, which is more general. Then some problems needing to be concerned along with two diagnostic methods used in diagnostic system are introduced. Finally, two programmable flow charts for the two methods are given and corresponding examples demonstrate their feasibility in practice.展开更多
Interferometric phase filtering is one of the key steps in interferometricsynthetic aperture radar (InSAR/SAR). However, the ideal filtering results are difficult toobtain due to dense fringe and low coherence regions...Interferometric phase filtering is one of the key steps in interferometricsynthetic aperture radar (InSAR/SAR). However, the ideal filtering results are difficult toobtain due to dense fringe and low coherence regions. Moreover, the InSAR/SAR datarange is relatively large, so the efficiency of interferential phase filtering is one of themajor problems. In this letter, we proposed an interferometric phase filtering methodbased on an amended matrix pencil and linear window mean filter. The combination ofthe matrix pencil and the linear mean filter are introduced to the interferometric phasefiltering for the first time. First, the interferometric signal is analyzed, and theinterferometric phase filtering is transformed into a local frequency estimation problem.Then, the local frequency is estimated using an amended matrix pencil at a window. Thelocal frequency can represent terrain changes, thus suggesting that the frequency can beaccurately estimated even in dense fringe regions. Finally, the local frequency is filteredby using a linear window mean filter, and the filtered phase is recovered. The proposedmethod is calculated by some matrices. Therefore, the computational complexity isreduced, and the efficiency of the interferometric phase filtering is improved.Experiments are conducted with simulated and real InSAR data. The proposed methodexhibits a better filtering effect and an ideal efficiency as compared with the traditionalfiltering method.展开更多
A new beam broadening synthesis technique for Synthetic Aperture Radar(SAR) antenna array, namely Projection Matrix Algorithm(PMA) is presented. The theory of PMA is introduced firstly, and then the iterative renewed ...A new beam broadening synthesis technique for Synthetic Aperture Radar(SAR) antenna array, namely Projection Matrix Algorithm(PMA) is presented. The theory of PMA is introduced firstly, and then the iterative renewed manner is improved to resolve the unbalance problem under amplitude and phase control. In order to validate the algorithm correct and effective, an actual engineering application example is investigated. The beam synthesis results of 1.0~4.5 times broadening under the phase only control and the amplitude and phase control using improved PMA are given. The results show that the beam directivity, the beam broadening, and the side-lobe level requirements were met. It is demonstrated that the improved PMA was effective and feasible for SAR application.展开更多
This paper presents a new method for constructing synthetic matrix which is obtained by extending the given judgement matrices in the Analytic Hierarchy Process (AHP). The consistency relationship among the given matr...This paper presents a new method for constructing synthetic matrix which is obtained by extending the given judgement matrices in the Analytic Hierarchy Process (AHP). The consistency relationship among the given matrices and their synthetic matrix is studied. The method can be used to deal with situations where the number of the given alternatives is larger than nine, and needs less pairwise comparisons than any others. Thus it will aid in the design of the AHP, which will reduce the information overload of decision maker, a major drawback of the original AHP algorithm. Finally, a numerical example is given to show the feasibility and effectiveness of the method.展开更多
The new geometrical constraints, based on the geometrical analysing of synthetic method, are developed to estimate fundamental matrix (F matrix). Applying the new constraints, the four parameters of fundamental matrix...The new geometrical constraints, based on the geometrical analysing of synthetic method, are developed to estimate fundamental matrix (F matrix). Applying the new constraints, the four parameters of fundamental matrix could be estimated firstly, and these four parameters are the coordinates of the two epipoles. The other four parameters of the fundamental matrix could be solved by solving the linear equations with the other new constraint secondly, and these parameters represent the homography between the two pencils of epipolar lines. The synthetic data and the real data are used to test the new method. And the method is of the advantages of obvious geometrical meaning, and high stability of the epipoles of the fundamental matrix.展开更多
文摘Ovarian follicle growth in three dimensional (3D) matrices in vitro has limitations: a) matrices don’t expand as follicles grow, b) requirements for enzyme-mediated retrieval, and c) animal-derived components prevent clinical application. Therefore, we evaluated N-Isopropylacrylamide (SFX-1), a novel synthetic 3D culture matrix, for follicle culture. Groups of three murine secondary follicles were encapsulated in 50 μL of DMEM/F12-1%ITS-10%FCS (DMEM/F12) or SFX-1 (3:2 v/v DMEM/F12) or Matrigel (1:1 DMEM/F12) and cultured for 48 h. Matrigel contains growth factors but SFX-1 has no animal-derived factors. Each culture condition was examined in 6 wells containing 18 follicles, in four replicate experiments (n = 4). Photomicrographs were used to determine follicle diameters and morphological integrity. Follicles were Live-Dead (LD) stained or disaggregated to generate cells for viability assessment using Trypan Blue (TB). Estradiol, progesterone and anti-mullerian hormone (AMH) in conditioned media were measured using Enzyme-linked Immunoassay. All culture conditions supported similar increases in follicle diameter. DMEM/F12 did not maintain morphological integrity which prevented follicle retrieval after 48 h;25% were retrieved from DMEM/F12, but 44% and 41% follicles were retrieved from SFX-1 and Matrigel respectively. Follicles retrieved from Matrigel could not be disaggregated, which prevented TB viability assessment. LD estimations of viable cells/follicle were lower than TB, but culture conditions had no effect on viability;SFX-1 64% ± 8% and DMEM/F12 69% ± 9%. SFX-1 and Matrigel supported similar levels of progesterone synthesis, only Matrigel supported estrogen synthesis, but none of the culture conditions supported AMH production. SFX-1 was not cytotoxic and was comparable to Matrigel. Further development of SFX-1 for use with human follicles is supported.
文摘On-line measurement for dielectric loss angle can effectively monitor the insulation condition of capacitive equipment in power systems. Synthetic relative measuring methods not only markedly overcome the shortcomings of traditional absolute measuring methods but also greatly improve the accuracy of dielectric loss angle measurement. However, synthetic relative measuring methods based on two or three pieces of capacitive equipment do not have the characteristic of generality. In this paper, a principle of synthetic relative measuring method is presented. The example of application for synthetic relative methods based on three and four pieces of capacitive equipment running in the same phase is taken to present the failure judgment matrices for N pieces of equipment. According to these matrices, the fault condition of N pieces of capacitive equipment can be watched, which is more general. Then some problems needing to be concerned along with two diagnostic methods used in diagnostic system are introduced. Finally, two programmable flow charts for the two methods are given and corresponding examples demonstrate their feasibility in practice.
基金The authors would like to thank the support by the State Key Program of National Natural Science Foundation of China under Grant[Number 41774026]the Satellite Mapping Technology and Application,National Administration of Surveying,Mapping and Geoinformation Key Laboratory under Grant[Number KLSMTA-201708].
文摘Interferometric phase filtering is one of the key steps in interferometricsynthetic aperture radar (InSAR/SAR). However, the ideal filtering results are difficult toobtain due to dense fringe and low coherence regions. Moreover, the InSAR/SAR datarange is relatively large, so the efficiency of interferential phase filtering is one of themajor problems. In this letter, we proposed an interferometric phase filtering methodbased on an amended matrix pencil and linear window mean filter. The combination ofthe matrix pencil and the linear mean filter are introduced to the interferometric phasefiltering for the first time. First, the interferometric signal is analyzed, and theinterferometric phase filtering is transformed into a local frequency estimation problem.Then, the local frequency is estimated using an amended matrix pencil at a window. Thelocal frequency can represent terrain changes, thus suggesting that the frequency can beaccurately estimated even in dense fringe regions. Finally, the local frequency is filteredby using a linear window mean filter, and the filtered phase is recovered. The proposedmethod is calculated by some matrices. Therefore, the computational complexity isreduced, and the efficiency of the interferometric phase filtering is improved.Experiments are conducted with simulated and real InSAR data. The proposed methodexhibits a better filtering effect and an ideal efficiency as compared with the traditionalfiltering method.
文摘A new beam broadening synthesis technique for Synthetic Aperture Radar(SAR) antenna array, namely Projection Matrix Algorithm(PMA) is presented. The theory of PMA is introduced firstly, and then the iterative renewed manner is improved to resolve the unbalance problem under amplitude and phase control. In order to validate the algorithm correct and effective, an actual engineering application example is investigated. The beam synthesis results of 1.0~4.5 times broadening under the phase only control and the amplitude and phase control using improved PMA are given. The results show that the beam directivity, the beam broadening, and the side-lobe level requirements were met. It is demonstrated that the improved PMA was effective and feasible for SAR application.
基金This research is supported by the National Natural Science Foundation of China(No.79970093).
文摘This paper presents a new method for constructing synthetic matrix which is obtained by extending the given judgement matrices in the Analytic Hierarchy Process (AHP). The consistency relationship among the given matrices and their synthetic matrix is studied. The method can be used to deal with situations where the number of the given alternatives is larger than nine, and needs less pairwise comparisons than any others. Thus it will aid in the design of the AHP, which will reduce the information overload of decision maker, a major drawback of the original AHP algorithm. Finally, a numerical example is given to show the feasibility and effectiveness of the method.
基金Project supported by the National Natural Science Foundation of China (Grant No. 69605002) France-China Advanced Research Program
文摘The new geometrical constraints, based on the geometrical analysing of synthetic method, are developed to estimate fundamental matrix (F matrix). Applying the new constraints, the four parameters of fundamental matrix could be estimated firstly, and these four parameters are the coordinates of the two epipoles. The other four parameters of the fundamental matrix could be solved by solving the linear equations with the other new constraint secondly, and these parameters represent the homography between the two pencils of epipolar lines. The synthetic data and the real data are used to test the new method. And the method is of the advantages of obvious geometrical meaning, and high stability of the epipoles of the fundamental matrix.
