Systemic Activation of Defensive Enzymes and Protection in Tobacco Plantlets against <i>Phytophthora nicotianae</i>Induced by Oligosaccharins

Oligosaccharins are potent biomolecules which activate defense responses and resistance in tobacco plants. However, it is not known the systemic behavior of defensive enzymes activated by these elicitors. In this work, the dynamic behavior of key defensive enzymes was evaluated in tobacco plant leaves previously treated through the roots with chitosan polymer (CH), chitosan (COS) and pectic (OGAS) oligosaccharides and Spermine (Sp). All macromolecules tested activated protein levels and defense enzymatic activity in tobacco leaves but with different response dynamics among them and depending on the biochemical variable evaluated. Defense response above control levels were detected since 12 hours after treatments and it consisted in a biphasic behavior with two peaks for PAL (EC 4.3.1.5) and β 1 - 3 glucanase (EC 3.2.1.6) enzymatic activities. The highest enzymatic levels for these enzymes were achieved at 48 hours in plantlets elicited with COS and at 72 hours for those plants treated with chitosan polymer, while the highest POD (EC 1.11.1.6) activity was detected with CH between 48 and 72 hours. These results demonstrated systemic defense activation by oligosaccharins in tobacco whose dynamic of defense response is affected by the kind of oligosaccharins tested. When applying OGAS by foliar spray on tobacco, systemic resistance against Phytoththora nicotianae was induced and plantlets were protected with the low concentration tested by 46% under the bioassays conditions performed. Moreover, enzymatic determinations on roots and leaves previous to plant-pathogen interaction showed increments above 30% of control levels for PAL and POD activities. It means that oligosaccharins activate local and systemic defense responses in plants in the absent of pathogen infection.

Induced Systemic Resistance in Two Genotypes of <i>Brassica napus</i>(AACC) and <i>Raphanus oleracea</i>(RRCC) by <i>Trichoderma</i>Isolates against <i>Sclerotinia sclerotiorum</i>

Two different species, Trichoderma viride TV10 and Trichoderma harzianum TH12 from 30 Trichoderma isolates were selected out based on their high growth inhibition of the phytopathogen Sclerotinia sclerotiorum (Lib) de Bary, which reached 84.44% and 100%, respectively. Their untreated culture filtrates (CF) and culture filtrates treated with heat (CFH) also were tested for growth inhibition of the pathogen in potato dextrose agar (PDA). Morphological and molecular characterisation by internal transcribed spacer (ITS) PCR provided consistent identification of these isolates. The degree of infection and disease index (DI) of S. sclerotiorum were examined in Brassica napus (AACC) and Raphanus alboglabra (RR) and Brassica alboglabra (CC). The results revealed that Raphanus alboglabra showed higher disease resistance than that of B. napus. Biotic elecitors T. harzianum TH12 and T. viride TV10 and their CF and CFH demonstrated the ability to cause induced systemic resistance (ISR) in B. napus and Raphanus alboglabra against sclerotinia stem rot (SSR) disease. Furthermore, a high ability to reduce the degree of infection and DI in B. napus with the biotic elicitors T. harzianum TH12 and T. viride TV10 was observed, with numbers reaching 7.22% to 6.67% and 17.78% to 11.67%, respectively. When CF were used, reached 20.00% to 16.67% and 33.33% to 23.33%, respectively;with CFH, values reached 35.00% to 21.67% and 37.78% to 28.33%, respectively. While in Raphanus alboglabra the degree of infection and DI reached 0.00% and 0.00% with all biotic elicitors treatments. These results show that biotic elicitor treatments significantly (P B. napus and Raphanus alboglabra ranked as most effective. This study showed for the first time the ability of genotype Raphanus alboglabra (RRCC) to demonstrate resistance against S. sclerotiorum with or without treatment by biotic elicitors and the ability of genotype B. napus (AACC) to demonstrate resistance to the pathogen after treatment with biotic elicitors.

Genetic improvement of Trichoderma ability to induce systemic resistance

The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity.

L-leap:accelerating the stochastic simulation of chemically reacting systems

Presented here is an L-leap method for accelerating stochastic simulation of well-stirred chemically reacting systems, in which the number of reactions occurring in a reaction channel with the largest propensity function is calculated from the leap condition and the number of reactions occurring in the other reaction channels are generated by using binomial random variables during a leap. The L-leap method can better satisfy the leap condition. Numerical simulation results indicate that the L-leap method can obtain better performance than established methods.

A Review on Chemical Effects in Aqueous Solution induced by Plasma with Glow Discharge

Chemical effects in different aqueous solutions induced by plasma with glow discharge electrolysis (GDE) and contact glow discharge electrolysis (CGDE) are described in this paper. The experimental and discharge characteristics are also reviewed. These are followed by a discussion of their mechanisms of both anodic and cathodic CGDE..

Theoretical study of the intensity of chemically induce dynamic electron polarization of radical-triplet pairs

Considering the interaction between excited triplet molecule and doublet radical, based on the second-order perturbation theory and the motion equation of density matrix, the polarization intensity of RTPM were theoretically calculated with the overpopulated doublet spin states and quartet spin states of radical-triplet pairs as initial conditions respectively. The results of calculation indicate that the net emissive polarization and the net absorptive polarization on the radical result from the zero-field-splitting (zfs) and the multiplet A/E and E/A polarization result from hyperfine (hf) interactions of the triplet molecule. The hyperfine related A+A/E or E+E/A CIDEP on the radical were the overpopulation of the net absorptive or emissive polarization and multiplet A/E or E/A polarization..

Laser-induced voltage effects in Ca_3Co_4O_9 thin films on tilted LaAlO_3(001) substrates grown by chemical solution deposition

Laser-induced voltage effects in c-axis oriented Ca3Co4O9 thin films have been studied with samples fabricated on 10°tilted LaAIO3 （001） substrates by a simple chemical solution deposition method. An open-circuit voltage with a rise time of about 10 ns and full width at half maximum of about 28 ns is detected when the film surface is irradiated by a 308-nm laser pulse with a duration of 25 ns. Besides, opemcircuit voltage signals are also observed when the film surface is irradiated separately by the laser pulses of 532 nm and 1064 nm. The results indicate that Ca3Co4O9 thin films have a great potential application in the wide range photodetctor from the ultraviolet to near infrared regions.

Expression of Inducible Co-stimulator in Peripheral Blood T Lymphocytes in the Patients with Systemic Lupus Erythematosus

The expression of inducible co-stimulator (ICOS) in peripheral blood T lymphocytes from the patients with systemic lupus erythematosus (SLE) and the role in the pathogenesis of SLE was investigated. By using two-color immunofluorescent staining and flow cytometric assay, the expression levels of ICOS in peripheal blood T lymphocytes from 33 patients with SLE and 16 healthy volunteers were detected. SLE diseases activity index (SLEDAI) of the patients with SLE was used to evaluate the disease activity. The correlation between the ICOS expression and SLEDAI was analyzed among the groups. The results showed that the expression levels of ICOS in T lymphocytes in active SLE group was markedly higher than those in the control and inactive SLE groups (both P<0.01). There was no significant difference in the expression levels of ICOS between the inactive SLE and the control groups (P>0.05). In active SLE and inactive SLE groups, positive linear correlation was found between the levels of the ICOS expression in T lymphocytes and SLEDAI (r=0.711, P=0.001; r=0.561, P=0.03). It was suggested that the expression of ICOS in peripheral blood T lymphocytes from the patients with active SLE was up-regulated and and ICOS might be related to the pathogenesis of SLE.

A kind of noise-induced transition to noisy chaos in stochastically perturbed dynamical system

We investigate a kind of noise-induced transition to noisy chaos in dynamical systems. Due to similar phenomenological structures of stable hyperbolic attractors excited by various physical realizations from a given stationary random process, a specific Poincar6 map is established for stochastically perturbed quasi-Hamiltonian system. Based on this kind of map, various point sets in the Poincar6＇s cross-section and dynamical transitions can be analyzed. Results from the customary Duffing oscillator show that, the point sets in the Poincare＇s global cross-section will be highly compressed in one direction, and extend slowly along the deterministic period-doubling bifurcation trail in another direction when the strength of the harmonic excitation is fixed while the strength of the stochastic excitation is slowly increased. This kind of transition is called the noise-induced point-overspreading route to noisy chaos.

Stochastic stability of networked control systems with network-induced delay and data dropout

This paper deals with the stochastic stability of networked control systems with the presence of network- induced delay and transmitted data dropout. Based on the Lyapunov approach, sufficient conditions for the mean-square stability of the networked control system are derived subject that the sequence of transmission interval is driven by an identically independently distributed sequence and by a finite state Markov chain, respectively. Stabilization controllers are constructed in terms of linear matrix inequalities correspondingly. An example is provided to illustrate our results.

Shock Induced Chemical Reactions of Intermetallic Mixture of Nickel and Aluminum and Associated Transition States

In this paper, numerical simulation of shock-induced chemical reactions of intermetallic mixtures is discussed. Specifically, the paper focuses on intermetallic mixture of nickel and aluminum. To initiate the chemical reactions, the thermal input or the shockwave should supply the energy to take the reactants, mixture of nickel and aluminum, to the transition state. Thus, for any numerical simulation or analysis of the shock or thermally induced chemical reaction in a continuum scale or a meso scale, it is necessary to identify the transition state. The transition state for the intermetallic mixture of nickel and the aluminum is identified in this paper and a result of the numerical simulation of the shock-induced chemical reaction, in a continuum scale is presented. The numerical solutions clearly show the chemical reactions, release of heat energy, increase of the temperature and the formation of products, following the transition state and the resulting shock-induced chemical reaction of a binary intermetallic energetic mixture of nickel and aluminum. The studies also show that the collapse of porosity is a mechanism that takes the reactants to the transition state, in shock-induced chemical reactions of binary intermetallic mixtures.

Electromagnetically induced grating in a thermal N-type four-level atomic system

The electromagnetically induced grating effect in thermal and cold atoms has been studied theoretically. Studies have shown that, by adjusting the parameters, the first-order diffraction efficiency of the probe beam in the cold atomic system and the thermal atomic system is 34% and 31%, respectively, which is very close to the ideal diffraction efficiency of the sinusoidal grating. However, it is more difficult to prepare the cold atomic system than to prepare the thermal atomic system in the practical application, so the study of the electromagnetically induced grating effect in the thermal atomic system may be helpful for practical applications.

A NEW CHEMICAL OSCILLATOR INDUCED BY SURFACTANT WITH GLUCOSE AS THE SUBSTRATE

A new Belousov-Zhabotinsky oscillator induced by Triton X-100 (a nonionic surfactant)with glucose as the organic substrate and Ce^(4+)as the catalyst has been reported in this paper.

Evaluation of Selected Chemical,Biological Fungicides,and Induced Resistance to Control White Rot(Sclerotium rolfsii Sacc.)on Tomato

Tomato(Solanum lycopersicum)is one of the most widely grown and consumed as fresh vegetable in the world.Sclerotium rolfsii Sacc.is one of the most destructive diseases and affects more than 500 plant species.This study was conducted for“Evaluation of Selected Chemical,Biological Fungicides,and Induced Resistant to Control White Rot(Sclerotium rolfsii Sacc.)on Tomato”.The experiment was conducted at the Royal University of Agriculture and divided into two sections.The first section conducted under in vitro condition consists of four treatments T1 control treatment,T2 copper hydroxide,T3 azoxystrobin+difenoconazole,T4 metalaxyl+mancozeb in Nagoya Laboratory,and had been starting from 11st May to 15th May 2020.Section two was conducted in a pot in net-house which started from 1st January 2019 to 19th February 2019 and 1st January to 19th February 2020 and arranged in RCBD(Randomized Completely Block Design)with six replications and nine treatments.T0 negative control,T1 inoculate but not treatment,T2 metalaxyl+mancozeb,T3 azoxystrobin+difenoconazole,T4 copper hydroxide,T5 acibenzolar-S-methyl,T6 chicken dung+Trichoderma harzianum,T7 acibenzolar-S-methyl+chicken dung+Trichoderma harzianum,T8 acibenzolar-S-methyl+metalaxyl+mancozeb.Data were collected on colonizing diameter in vitro.For pot experiment collected on disease incidence,incubation period Based on the result under in vitro condition metalaxyl+mancozeb highly inhibited germination of Sclerotium rolfsii when compared with another treatment.However,the application of copper hydroxide seems less effective compared with control.For in pot experiment,when treated after symptom of southern blight appeared to seem less effective on the severity of southern blight.In contrast,when applied as a protectant and curative when symptoms appeared only systemic fungicide azoxystrobin+difenoconazole significantly reduced the severity of Sclerotium rolfsii and delayed incubation period while compared with other treatments(p<0.05)and was followed by metalaxyl+mancozeb,whereas,copper hydroxide,acibenzolar-S-methyl,chicken dung+Trichoderma harzianum,acibenzolar-S-methyl+chicken dung+Trichoderma harzianum,acibenzolar-S-methyl+metalaxyl+mancozeb were less effective(p>0.05).Based on the three experiments we can assume that fungicide is more effective in reducing the growth of the pathogen and delaying the incubation period of fungal colonization when compared with biological control and induced resistance.

Fabrication of micro carbon pillar by laser-induced chemical vapor deposition

Argon ion laser was used as the induced light source and ethane(C2H4) was selected as the precursor gas,in the variety ranges of laser power from 0.5 W to 4.5 W and the pressure of the precursor gas from 225×133.3 Pa to 680×133.3 Pa,the experiments of laser induced chemical vapor deposition were proceeded for fabrication of micro carbon pillar.In the experiments,the influences of power of laser and pressure of work gas on the diameter and length of micro carbon pillar were investigated,the variety on averaged growth rate of carbon pillar with the laser irradiation time and moving speed of focus was discussed.Based on experiment data,the micro carbon pillar with an aspect ratio of over 500 was built through the method of moving the focus.

Macroscopic effects in electromagnetically-induced transparency in a Doppler-broadened system

We study the electromagnetically-induced transparency（EIT） in a Doppler-broadened cascaded three-level system.We decompose the susceptibility responsible for the EIT resonance into a linear and a nonlinear part, and the EIT resonance reflects mainly the characteristics of the nonlinear susceptibility. It is found that the macroscopic polarization interference effect plays a crucial role in determining the EIT resonance spectrum. To obtain a Doppler-free spectrum there must be polarization interference between atoms of different velocities. A dressed-state model, which analyzes the velocities at which the atoms are in resonance with the dressed states through Doppler frequency shifting, is employed to explain the results.

Opto-electromechanically induced transparency in a hybrid opto-electromechanical system

We study opto-electromechanically induced transparency in a hybrid opto-electromechanical system made up of an optical cavity tunneling-coupled to an opto-mechanical cavity, which is capacitively coupled to a charged mechanical oscillator by a charged and moveable mechanical cavity mirror as an interface. By studying the effects of the different parameters on the output field, we propose a scheme to modulate the opto-electromechanically induced transparency(OEMIT). Our results show that the OEMIT with the transparency windows from single to double to triple can be modulated by changing the tunneling, opto-mechanical and electrical couplings. In addition, the explanation of the OEMIT with multi-windows is given by the energy level diagram based on quantum interference. Our investigation will provide an optimal platform to manipulate the transmission of optical field via microfabricated opto-electromechanical device.

Constraint-induced movement therapy promotes motor function recovery and downregulates phosphorylated extracellular regulated protein kinase expression in ischemic brain tissue of rats

Motor function impairment is a common outcome of stroke.Constraint-induced movement therapy（CIMT）involving intensive use of the impaired limb while restraining the unaffected limb is widely used to overcome the effects of＇learned non-use＇and improve limb function after stroke.However,the underlying mechanism of CIMT remains unclear.In the present study,rats were randomly divided into a middle cerebral artery occlusion（model）group,a CIMT＋model（CIMT）group,or a sham group.Restriction of the affected limb by plaster cast was performed in the CIMT and sham groups.Compared with the model group,CIMT significantly improved the forelimb functional performance in rats.By western blot assay,the expression of phosphorylated extracellular regulated protein kinase in the bilateral cortex and hippocampi of cerebral ischemic rats in the CIMT group was significantly lower than that in the model group,and was similar to sham group levels.These data suggest that functional recovery after CIMT may be related to decreased expression of phosphorylated extracellular regulated protein kinase in the bilateral cortex and hippocampi.

Phase-dependent double optomechanically induced transparency in a hybrid optomechanical cavity system with coherently mechanical driving

We propose a scheme that can generate tunable double optomechanically induced transparency in a hybrid optomechanical cavity system.In this system, the mechanical resonator of the optomechanical cavity is coupled with an additional mechanical resonator and the additional mechanical resonator can be driven by a weak external coherently mechanical driving field.We show that both the intensity and the phase of the external mechanical driving field can control the propagation of the probe field, including changing the transmission spectrum from double windows to a single-window.Our study also provides an effective way to generate intensity-controllable, narrow-bandwidth transmission spectra, with the probe field modulated from excessive opacity to remarkable amplification.

Triple optomechanical induced transparency in a two-cavity system

We theoretically investigate the optomechanical induced transparency（OMIT） phenomenon in a two-cavity system which is composed of two optomechanical cavities. Both of the cavities consist of a fixed mirror and a high-Q mechanical resonator, and they couple to each other via a common waveguide. We show that in the presence of a strong pump field applied to one cavity and a weak probe field applied to the other, a triple-OMIT can be observed in the output field at the probe frequency. The two mechanical resonators in the two cavities are identical, but they lead to different quantum interference pathways. The transparency windows are induced by the coupling of the two cavities and the optical pressure radiated to the mechanical resonators, which can be controlled via the power of the pump field and the coupling strength of the two cavities.