It is necessary to develop a new strategy for treatment of lung cancer since it is the main cause of cancer death.Tanshinone IIA(Tan IIA),an active ingredient of a commonly used traditional Chinese herb Salvia miltior...It is necessary to develop a new strategy for treatment of lung cancer since it is the main cause of cancer death.Tanshinone IIA(Tan IIA),an active ingredient of a commonly used traditional Chinese herb Salvia miltiorrhiza,provides a new direction to develop a new strategy for the treatment.It has been found that Tan IIA could inhibit lung cancer in vitro and in vivo by inducing autophagic apoptosis.Tan IIA increased apoptotic cells and the expression of cleaved caspase 3 and cleaved caspase 9,decreased B-cell lymphoma-2(Bcl-2)/Bcl-2 associated X protein(Bax)ratio in human non-small cell lung cancer(NSCLC)cell lines,which was promoted by an autophagy activator Rapamycin,and weaken by autophagy inhibitor 3-methyladenine(3-MA).In addition,Tan IIA induced more autophagosomes,up-regulated light chain 3b(LC-3B)I and LC-3B II conversion and less sequestosome 1(SQSTM1/p62)expression,which cannot be weakened by the caspase 3 antagonist.Moreover,overexpression of LC-3B gene(LC3B)and downregulation of autophagic gene 5(ATG5)further confirmed that Tan IIA induced autophagic apoptosis in NSCLC cell lines.Beclin-1 gene(BECN1)overexpression and silence attenuated the effects of Tan IIA,suggesting autophagic apoptosis that Tan IIA induced was dependent on Beclin-1.Overall,our study demonstrated a new treatment mechanism of Tan IIA and suggested that Tan IIA is a potential new anti-cancer therapeutic option.展开更多
[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Ta...[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.展开更多
Studying the covers of the three editions of The Kitchen God’s Wife by Amy Tan,a famous female Chinese American writer,the author finds Chinese cultural elements stood out in publishers’earliest recommendations,but ...Studying the covers of the three editions of The Kitchen God’s Wife by Amy Tan,a famous female Chinese American writer,the author finds Chinese cultural elements stood out in publishers’earliest recommendations,but later were replaced by immigrant mother-daughter relationship,and then were overwhelmed by the concern for human beings.It is interesting that this change fits the changing focuses of Chinese American writers in the 21st century.展开更多
Entrepreneurship is one of the popular choices for students after graduation from higher education.The study focuses on proposing a model to identify the factors affecting the entrepreneurial intention of students at ...Entrepreneurship is one of the popular choices for students after graduation from higher education.The study focuses on proposing a model to identify the factors affecting the entrepreneurial intention of students at Tan Trao University by researching some typical models in the world.By quantitative research methods based on a survey of 391 students,the study has shown that there are 5 factors affecting the entrepreneurial intention of students at Tan Trao University,including opportunity,motivation,perception of behavioral control,skills and resources for entrepreneurship.Thereby,the study proposes a number of solutions for the students,the university and other related parties to promote students’entrepreneurial intentions.展开更多
基金The study was supported by grants from the National Natural Science Foundation of China(81903943 and 82074219)the Natural Science Foundation of Guangdong Province(2017A030310502)+1 种基金The study was also supported by the Key Projects of Educational Commission of Guangdong Province(2018KZDXM020)the Science and Technology Planning Project of Guangzhou(202002030009).
文摘It is necessary to develop a new strategy for treatment of lung cancer since it is the main cause of cancer death.Tanshinone IIA(Tan IIA),an active ingredient of a commonly used traditional Chinese herb Salvia miltiorrhiza,provides a new direction to develop a new strategy for the treatment.It has been found that Tan IIA could inhibit lung cancer in vitro and in vivo by inducing autophagic apoptosis.Tan IIA increased apoptotic cells and the expression of cleaved caspase 3 and cleaved caspase 9,decreased B-cell lymphoma-2(Bcl-2)/Bcl-2 associated X protein(Bax)ratio in human non-small cell lung cancer(NSCLC)cell lines,which was promoted by an autophagy activator Rapamycin,and weaken by autophagy inhibitor 3-methyladenine(3-MA).In addition,Tan IIA induced more autophagosomes,up-regulated light chain 3b(LC-3B)I and LC-3B II conversion and less sequestosome 1(SQSTM1/p62)expression,which cannot be weakened by the caspase 3 antagonist.Moreover,overexpression of LC-3B gene(LC3B)and downregulation of autophagic gene 5(ATG5)further confirmed that Tan IIA induced autophagic apoptosis in NSCLC cell lines.Beclin-1 gene(BECN1)overexpression and silence attenuated the effects of Tan IIA,suggesting autophagic apoptosis that Tan IIA induced was dependent on Beclin-1.Overall,our study demonstrated a new treatment mechanism of Tan IIA and suggested that Tan IIA is a potential new anti-cancer therapeutic option.
基金Supported by Ningxia Agricultural Reclamation Group Science and Technology Innovation ProjectNew Feed Technology Promotion Project of Ningxia Agriculture and Rural DepartmentNingxia Feed Industry Expert Technical Service Group Project。
文摘[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.
基金USST International Professional Certification and All-English Course Construction,USST Postgraduate Teaching Construction Project.
文摘Studying the covers of the three editions of The Kitchen God’s Wife by Amy Tan,a famous female Chinese American writer,the author finds Chinese cultural elements stood out in publishers’earliest recommendations,but later were replaced by immigrant mother-daughter relationship,and then were overwhelmed by the concern for human beings.It is interesting that this change fits the changing focuses of Chinese American writers in the 21st century.
文摘Entrepreneurship is one of the popular choices for students after graduation from higher education.The study focuses on proposing a model to identify the factors affecting the entrepreneurial intention of students at Tan Trao University by researching some typical models in the world.By quantitative research methods based on a survey of 391 students,the study has shown that there are 5 factors affecting the entrepreneurial intention of students at Tan Trao University,including opportunity,motivation,perception of behavioral control,skills and resources for entrepreneurship.Thereby,the study proposes a number of solutions for the students,the university and other related parties to promote students’entrepreneurial intentions.
