In this paper,a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported.A tandem genes cluster CHS-CHI-IFS(rIFS)of secondary metabolites of plant isoflavones was con...In this paper,a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported.A tandem genes cluster CHS-CHI-IFS(rIFS)of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase(CHS),chalcone isomerase(CHI),and isoflavone synthase(IFS)(GenBank accession numbers EU526827,EU526829,EU526830)in only one recombination with the pET22b vector.The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions,and the genistein-like com-ponent was detected.展开更多
文摘In this paper,a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported.A tandem genes cluster CHS-CHI-IFS(rIFS)of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase(CHS),chalcone isomerase(CHI),and isoflavone synthase(IFS)(GenBank accession numbers EU526827,EU526829,EU526830)in only one recombination with the pET22b vector.The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions,and the genistein-like com-ponent was detected.
