[Objective] The paper was to measure and analyze pathogen of 18S rDNA sequence of the pathogen of melon powdery mildew from lands overlaid with sands. [Method]The melon powdery mildew was isolated from infected plants...[Objective] The paper was to measure and analyze pathogen of 18S rDNA sequence of the pathogen of melon powdery mildew from lands overlaid with sands. [Method]The melon powdery mildew was isolated from infected plants of ＂Yujinxiang＂, a major melon variety cultivated in lands overlaid with sands in the middle arid area of Ningxia. Genome DNA was extracted from its conidia using Chelex-100 method. 18S rDNA sequence was amplified by PCR, which was analyzed by Blast after sequencing, and the phylogenetic tree was constructed. [Result] 18S rDNA sequence analysis showed that the pathogen of melon powdery mildew belonged to Podosphaera. [Conclusion] The study provided reference for biocontrol and disease-resistance breeding against melon powdery mildew.展开更多
Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and ...Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction （PCR） and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers （ITS） sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the Mvnl specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.展开更多
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ0958)
文摘[Objective] The paper was to measure and analyze pathogen of 18S rDNA sequence of the pathogen of melon powdery mildew from lands overlaid with sands. [Method]The melon powdery mildew was isolated from infected plants of ＂Yujinxiang＂, a major melon variety cultivated in lands overlaid with sands in the middle arid area of Ningxia. Genome DNA was extracted from its conidia using Chelex-100 method. 18S rDNA sequence was amplified by PCR, which was analyzed by Blast after sequencing, and the phylogenetic tree was constructed. [Result] 18S rDNA sequence analysis showed that the pathogen of melon powdery mildew belonged to Podosphaera. [Conclusion] The study provided reference for biocontrol and disease-resistance breeding against melon powdery mildew.
基金supported by the National Natural Science Foundation of China (No.30571208)the Key Scientific and Technological Project of Hangzhou City (No.200432239),China
文摘Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction （PCR） and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers （ITS） sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the Mvnl specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.
