Comparison between the Medullary Indexes of Hairs from Apodemus orestes and A.draco,with Discussion about the Taxonomic Status of A.orestes

The taxonomic status of long-tailed field mouse (Apodemus orestes) is still uncertain up to now.Some scholars regarded it as a subspecies or a synonym of dragon field mouse (A.draco),while other scholars considered it a valid species.In the study,five specimens of adult long-tailed field mouse,dragon field mouse and Chevrier's field mouse (A.chevrieri) (as a contrast) were chosen respectively.After treated,with the eyepiece micrometer in the inverted microscope,we made measurements of the widths of hairs and medullas and then calculated the medullary indexes of straight guard hairs from around the mouth,head,back,abdomen and forelimb of each species.The results indicate that,viewing at each of the five parts and the mixture of the five parts,there are no significant differences between A.orestes and A.draco,Significant differences can be found between A.orestes & A.chevrieri and A.draco & A.chevrieri though.The standpoint that A.orestes was listed as a valid species is not supported by this study.

The Taxonomic Status of Spermophilus in the Plague Area of Dingbian County,Shaanxi Province,China

This study was conducted to define the taxonomic status of Spermophilus in the plague area of Dingbian County in Shaanxi Province,China,through the two-factor variance analysis of morphological characteristics,DNA barcoding,and chromosome karyotype analysis.

Taxonomic Status of Daduhe Loquat （Eriobotrya prinoides var. dadunensis）

The taxonomic status of Daduhe loquat （E. prinoides var. dadunensis） was studied through analyzing genetic relationships among Oakleaf loquat （E. prinoides）, Daduhe loquat and Common loquat （E. japonica） using inter simple sequence repeats （ISSR） molecular marker and morphologic marker in this paper. Based on ISSR marker research, the similarity coefficient between Oakleaf loquat and Common loquat was lower than the similarity coefficient between Oakleaf loquat and Daduhe loquat while the similarity coefficient between Daduhe loquat and Common loquat was intermediate. The highest additivity was obtained when Daduhe loquat was regarded as the undetermined hybrid （45.8%）. The specific bands of Oakleaf loquat and Common loquat were present in Daduhe loquat. Based on morphologic traits research, Daduhe loquat was also between Oakleaf loquat and Common loquat but a little leaning to Oakleaf loquat. All the results support that Daduhe loquat was hybrid of Oakleaf loquat and Common loquat.

The Taxonomic Status of Gymnura bimaculata and G. japonica： Evidence from Mitochondrial DNA Sequences

Japanese butterfly ray Gymnura japonica from twinspot butterfly ray G. bimaculata based on a pair of white spots behind eyes or not, which was not reliable. To clarify the taxonomic status of G. japonica and G. bimaculata, the nucleotide variation between the two butterfly rays was examined using mitochondrial DNA sequence comparisons. Approximately 585 bp of 16S ribosomal RNA （rRNA） and 1,128 bp cytochrome b （cyt b） genes were sequenced from representatives of two butterfly rays species in East China Sea. The results showed that there were the same sequences of 16S rRNA gene between two butterfly rays; six sites were variable among two butterfly rays of cyt b genes, the proportion of polymorphie loci was 0.53%, and two haplotypes were defined which genetic distance was 0.5%. Combined with the morphological character and the analysis of mtDNA sequence indicated that twinspot butterfly ray G. bimaculata was a synonym of Japanese butterfly ray G. japonica.

Taxonomic and genetic status of lancelet in Weihai coastal waters based on mitochondrial DNA sequence

Lancelets (subphylum Cephalochordata) are a transitional species between invertebrates and vertebrates. They are currently listed in the Second Order of Protected Animals in China. Lancelets were first documented in the waters around the city of Weihai (Shandong, China) in 2002. However, little is known about the phylogeny of this population. We analyzed the sequences of cytochrome b (Cyt b) and cytochrome oxidase c subunit I (CO I) genes from samples collected from coastal waters in the cities of Weihai and Qingdao (~150 km to the south). We analyzed 176 sequences, of which 150 were novel sequences and 26 were obtained from GenBank. Our results suggest that (1) lancelets in the two cities belong to the species Branchiostoma japonicus and have a high level of genetic diversity; (2) there is a high level of gene flow and low level of genetic differentiation between lancelets from the two cities; (3) demographic expansion occurred an estimated 1.1 million years (Ma) ago (mid Pleistocene) for lancelets in Weihai-Qingdao; and (4) the divergence between B. belcheri and B. japonicus was estimated at between 37.75 Ma (early Oligocene)-46.5 Ma (late Eocene).

Origin and phylogenetic analysis of Tibetan Mastiff based on the mitochondrial DNA sequence

At present, the Tibetan Mastiff is the oldest and most ferocious dog in the world. However, the origin of the Tibetan Mastiff and its phylogenetic relationship with other large breed dogs such as Saint Bernard are unclear. In this study, the primers were designed accord- ing to the mitochondrial genome sequence of the domestic dog, and the 2,525 bp mitochondrial sequence, containing the whole sequence of Cytochrome b, tRNA-Thr, tRNA-Pro, and control region of the Tibetan Mastiff, was obtained. Using grey wolves and coyotes as out- groups, the Tibetan Mastiff and 12 breeds of domestic dogs were analyzed in phylogenesis. Tibetan Mastiff, domestic dog breeds, and grey wolves were clustered into a group and coyotes were clustered in a group separately. This indicated that the Tibetan Mastiff and the other domestic dogs originated from the grey wolf, and the Tibetan Mastiff belonged to Carnivora, Canidae, Canis, Canis lupus, Canis lupus familiaris on the animal taxonomy. In domestic dogs, the middle and small breed dogs were clustered at first; German Sheepdog, Swedish Elkhound, and Black Russian Terrier were clustered into one group, and the Tibetan Mastiff, Old English Sheepdog, Leonberger, and Saint Bernard were clustered in another group. This confirmed the viewpoint that many of the famous large breed dogs worldwide such as Saint Bernard possibly had the blood lineage of the Tibetan Mastiff, based on the molecular data. According to the substitution rate, we concluded that the approximate divergence time between Tibetan Mastiff and grey wolf was 58,000 years before the present （YBP）, and the approximate divergence time between other domestic dogs and grey wolf was 42,000 YBP, demonstrating that the time of origin of the Tibetan Mastiff was earlier than that of the other domestic dogs.

Diversity and conservation of Chinese wild begonias

Begonia, one of the most diverse plant taxa and the fifth or sixth largest angiosperm genus, consists of over1800 accepted species. The number of species recognized within this genus has greatly increased over the past 20 years, rising from 80 to 200 species in China alone. Based on recent field surveys, the number of begonia species in China is predicted to be between 250 and 300. Given the large number of begonia species that still remain to be described, further taxonomical work is urgently required. This is especially true for Chinese Begonia, in which there is a huge diversity of habitat, habit, plant size, leaf type, flower and fruit morphology, and most species are narrowly distributed in isolated habitats that are subject to negative disturbances from climate change, as well as agricultural and industrial activities. Although the conservation status for the majority of species has been evaluated using the standards of the International Union for Conservation of Nature, the results don't represent the truth in many species, and also about11.5% of which are data-absent. In addition, illegal collection and over-harvesting of wild begonias for ornamental or medicinal use has increased due to the rapid development of internet commerce. Far more often than predicted, these species should be categorized as rare and endangered and require immediate protection. Ex situ conservation of Chinese begonias started in 1995 and over 60% of the total species have been so far introduced into cultivation by several major botanical gardens in China. However, only few research institutions, limited funds and human resources have been involved in Begonia conservation;moreover, no project has conducted reintroduction. Therefore, more conservation-based work remains to be done. Improved conservation of Chinese begonias in the future depends on further field survey, an improved understanding of population diversity, and integrative approaches, including in situ and ex situ conservation, seed banking, and plant reintroduction. Species-targeted conservation zones should be established for endangered species excluded from the existing nature reserves. Additionally, laws pertaining to plant protection should be extended to prevent the illegal collection and transaction of wild plants, particularly for those species with unique habitats and small populations.

Phylogenetic relationship of Picea mongolica with other Picea species in the same area based on chloroplast gene variations

Picea mongolica is a conifer with a limited distribution in China and its taxonomic status is controversial.In order to explore genetic diff erences between P.mongolica and other nearby Picea species and to investigate its taxonomic status,phylogenetic relationships were analyzed between P.mongolica and Picea koraiensis,Picea meyeri and Picea wilsonii by three chloroplast gene sequences matK,chlB and atpA.The length of joint chloroplast sequence is 2379 bp.The fi fteen haplotypes were identifi ed by haplotype network analysis,among which two were major haplotypes and nine were unique.In addition,the genetic diversity of the sample collection species was inferred.Based on the haplotype network and Neighbor Joining phylogenetic tree analysis,P.mongolica was located on the basal clade of the phylogenetic tree which had more primitive taxa,and the genetic diversity of P.mongolica was highest.The signifi cant diff erences between P.mongolica and these other Picea species were identifi ed by this research.

Genetic and Morphological Variations within Laudakia microlepis(Blanford, 1874)(Sauria: Agamidae) Populations in Southeastern Iran with Description of a New Subspecies

A new subspecies of Laudakia microlepis（Blanford, 1874） is described, from Taftan Mountain in southeastern Iran, based on morphological and genetic characteristics. This new subspecies is distinguished from the nominal subspecies by having a yellowish head along with the following combination of characters： a relatively larger body size; different number of scales around middle of body; larger, weakly keeled, dorsal median scales arranged in 11–13 oblique longitudinal rows; 123–136 scales in a single row from posterior edge of gular fold to vent; 27–31 scales in the first complete whorl around the tail. The type locality of Laudakia microlepis taftanica ssp. nov., is hereby, reported as the Taftan Mountain, about 300 kilometer southeast of the nominal subspecies known range.