Taxonomy of Two New Peritrich Ciliates(Protozoa:Ciliophora)from Inland Saltwaters of China

The inland saltwater lakes harbor exceptional biodiversity.Here,two new species of solitary sessile peritrich ciliates were isolated from Qinghai Lake,the largest inland saltwater lake in China.Their morphology,ciliature,silverline system,and molecular phylogeny were investigated based on live observation,silver staining,and analysis of the small subunit ribosomal DNA(SSU rDNA).Vorticella paraglobosa sp.n.is characterized mainly by its obconical or elongate bell-shaped zooid,C-shaped macronucleus,single ventrally located contractile vacuole,two-rowed infundibular polykinety 3,and 28-38 silverlines between peristome and aboral tro-chal band and 10-15 between aboral trochal band and scopula.Vorticella cotyliformis sp.n.differs from its congeners mainly by its double-layered peristomial lip,cup-shaped zooid,J-shaped macronucleus,single ventrally positioned contractile vacuole,three-rowed infundibular polykinety 3,and 70-85 silverlines between peristome and aboral trochal band and 21-25 between aboral trochal band and scopula.The SSU rDNA sequences of the two new species were obtained,and the subsequent molecular phylogenetic analysis supported their taxonomic classification.

Molecular phylogeny and taxonomy of Phlomoides(Lamiaceae subfamily Lamioideae)in China:Insights from molecular and morphological data

Phlomoides,with 150-170 species,is the second largest and perhaps most taxonomically challenging genus within the subfamily Lamioideae(Lamiaceae).With about 60 species,China is one of three major biodiversity centers of Phlomoides.Although some Phlomoides species from China have been included in previous molecular phylogenetic studies,a robust and broad phylogeny of this lineage has yet to be completed.Moreover,given the myriad new additions to the genus,the existing infrageneric classification needs to be evaluated and revised.Here,we combine molecular and morphological data to investigate relationships within Phlomoides,with a focus on Chinese species.We observed that plastid DNA sequences can resolve relationships within Phlomoides better than nuclear ribosomal internal and external transcribed spacer regions(nrITS and nrETS).Molecular phylogenetic analyses confirm the monophyly of Phlomoides,but most previously defined infrageneric groups are not monophyletic.In addition,morphological analysis demonstrates the significant taxonomic value of eight characters to the genus.Based on our molecular phylogenetic analyses and morphological data,we establish a novel section Notochaete within Phlomoides,and propose three new combinations as well as three new synonyms.This study presents the first molecular phylogenetic analyses of Phlomoides in which taxa representative of the entire genus are included,and highlights the phylogenetic and taxonomic value of several morphological characters from species of Phlomoides from China.Our study suggests that a taxonomic revision and reclassification for the entire genus is necessary in the future.

The Black-winged Monarch(Monarcha frater):Geographic variation,taxonomy,a"new"population,and an enduring mystery in migration

The Black-winged Monarch(Monarcha frater)inhabits low-mid elevation forests across New Guinea and far northeastern Australia.Of its four recognized subspecies,M.f.canescens is migratory,breeding in tropical north-eastern Australia from approximately November–March.The non-breeding range of M.f.canescens,apart from some individuals overwintering in Australia,has been unknown for>100 years.Data from 194 New Guinean and Australian specimens and a literature review have not solved this mystery;the narrow latitudinal range of the intratropical migration may inhibit use of GPS loggers in resolving the migration route.Field work in central and western parts of southern New Guinea is needed.Specimen data,however,revealed an essentially overlooked population in north-western New Guinea east of the Bird's Head Peninsula phenotypically indistinguishable from nominate M.f.frater of that Peninsula.Future genomic analysis of the complex will be largely done from toepad skin because frozen tissue samples for DNA analysis exist only of four individuals of what we suggest should be elevated to species rank as M.periophthalmicus.We discuss how genomic analysis might distinguish among alternative taxonomic hypotheses of one,two or three species.We suggest English names for what we consider to be three diagnosable taxa worth recognizing at species rank in this group:Arfak Monarch corresponding to M.frater(Bird's Head Peninsula and the“new”population of north-western New Guinea);Pearly Monarch for M.canescens(Australia and still unknown non-breeding range),and Masked Monarch for M.periophthalmicus(including kunupi,elsewhere in New Guinea).

Phylogenomics and integrative taxonomy reveal two new species of Amana(Liliaceae)

Until now the genus Amana(Liliaceae),known as ’East Asian tulips’,has contained just seven species.In this study,a phylogenomic and integrative taxonomic approach was used to reveal two new species,Amana nanyueensis from Central China and A.tianmuensis from East China.A.nanyueensis resembles Amana edulis in possessing a densely villous-woolly bulb tunic and two opposite bracts,but differs in its leaves and anthers.Amana tianmuensis resembles Amana erythronioides in possessing three verticillate bracts and yellow anthers,but differs in aspects of its leaves and bulbs.These four species are clearly separated from each other in principal components analysis based on morphology.Phylogenomic analyses based on plastid CDS further support the species delimitation of A.nanyueensis and A.tianmuensis and suggests they are closely related to A.edulis.Cytological analysis shows that A.nanyueensis and A tianmuensis are both diploid(2n=2x=24),different from A edulis,which is either diploid(northern populations) or tetraploid(southern populations,2n=4x=48).The pollen morphology of A.nanyueensis is similar to other Amana species(single-groove germination aperture),but A.tianmuensis is quite different because of the presence of a sulcus membrane,which creates the illusion of double grooves.Ecological niche modelling also revealed a niche differentiation between A.edulis,A.nanyueensis and A.tianmuensis.

EU Taxonomy:Overview of All“Green”Economic Activities as of June 2023

In June,the EU Commission published an update of the economic activities that can be classified as“green”.The article provides an updated tabular overview of now 152 economic activities in total,covering 17 sectors.In particular,the table shows in which of the four different delegated regulations the technical screening criteria for the economic activities are located.These are necessary in order to determine whether a concrete economic activity is taxonomy aligned.

Taxonomy characterization and cadmium biosorption of fungus strain

The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.

基于带语义差别的模糊Taxonomy的交易数据库关联规则聚类

关联规则聚类是大量关联规则的一种有效组织方式，本文针对基于商品分类信息的规则聚类方法存在的不足进行了改进，同时考虑了不同层次间的项目语义差别，以及具有不同隶属度的项目细致语义差别，将商品分类树改进为模糊Taxonomy的有向无环图结构，该结构可以处理一个项目同时属于多个父结点的情况。我们充分考虑了有向无环图的性质，提出了带细致语义差别的模糊Taxonomy结构构建方法和相应的规则距离计算方法，其中，规则距离计算过程中的项集距离计算方法无需计算最佳匹配，因此，具有较小的时间开销。规则距离计算和聚类可视化试验结果表明了该方法的可扩展性和有效性，在规则的聚类计算上取得了较为满意的结果。

The Systematic Taxonomy of Chinese Endemic Genera in Hynobiidae

Hynobiids are exclusively in Asia. China is the main distribution of hynobiids,and has many rich species because of the diversity of habitat. In present paper,the introduction on the systematic taxonomy of Chinese endemic genera （Protohynobius,Batrachuperus,Liua,Pachyhynobius and Pseudohynobius） in Hynobiidae will improve the understanding on these genera.

Discussions on taxonomy of genus Betula in northeast China

Many researches have been carried out on the taxonomy of Betula in northeast of China, but the classification of species and subdivision of species had some divergences at all times, which led to a disorder for developing and utilizing resources of Betula. In this paper, species and subdivision of Betula were classified not only according to the wax sample of Betula but also the comprehensive taxonomy of population characters, geographical distribution, and the habitat. Theconclusion supported the Fu Pei-yuns?(1995) viewpoint of the taxonomy of Betula in the northeast of China. But B. mandshurica (Regel) Nakai should be considered as one single species, the name of B. platyphyla Suk.var phellodendroidesTung should be the synonym of B. platyphyla var platyphyla, and B. ermanii Cham.var.yingkiliensis liou et Wang should be incorporated into B. ermanii Cham. B. ovalifolia Rupr should not be regarded as a variety of B. fruticosa Pall. but as one single species.

Semantic overlay network for searching taxonomy-based data sources

Distributed data sources which employ taxonomy hierarchy to describe the contents of their objects are considered, and a super-peer-based semantic overlay network （SSON） is proposed for sharing and searching their data objects. In SSON, peers are dynamically clustered into many semantic clusters based on the semantics of their data objects and organized in the semantic clusters into a semantic overlay network. Each semantic cluster consists of a super-peer and more peers, and is only responsible for answering queries in its semantic subspace. A query is first routed to the appropriate semantic clusters by an efficient searching algorithm, and then it is forwarded to the specific peers that hold the relevant data objects. Experimental results indicate that SSON has good scalability and achieves a competitive trade-off between search efficiency and costs.

用传统分类表与叙词表编制Taxonomy的理论可行性研究

Taxonomy概念可以理解为一个用于支持浏览功能的、基于某一特定环境或领域的概念等级体系。它由等级结构和主题词两个基本元素组成,在形式上结合了分类法和叙词表的特点,为用传统分类法和叙词表编制Taxonomy提供了理论可能性。但由于分类法、叙词表和Taxonomy在性质与功能上均有不同之处,在利用传统分类法和叙词表编制Taxonomy过程中会遇到一些问题,首先,分类法和叙词表在主题范覆盖范围方面可能满足不了Taxonomy的要求;其次,分类法和叙词表的两个基本元素,等级结构和主题词在多大程度上可被利用,有待进一步实证。另外,如何将环境因素结合到Taxonomy类表中,是个有待补充的问题。

Taxonomy:定义、辨析和应用

文章旨阐释Taxonomy概念,包括三部分内容:Taxonomy概念的定义、Taxonomy与文献分类法、叙词表、本体和主题指南的区别与联系以及Taxonomy的具体应用。

Corporate Taxonomy的编制方法和原则——以EDB Corporate Taxonomy的编制为例

文章以EDB Corporate Taxonomy的编制为例,从实践角度论述了Corporate Tax-onomy的编制方法与原则,同时对编制过程中涉及到的如传统知识组织工具的利用、设计角度的考虑、支持软件和后期维护等问题进行了探讨。

Kuedinskie Kluchiki, a Unique Middle Permian Biota Locality as a Key-point for Reconstruction of Late Paleozoic Terrestrial Ecosystems of the Urals, Russia

Field work focused on the Kuedinskie Kluchiki locality, Perm region, Urals, Russia, which contains a rich assemblage of diverse fossil organisms including higher plants(equisetophytes, pteridosperms, ginkgophytes, conifers, vojnovskyans) represented by stems, leaves and reproductive organs;invertebrates(mollusks, arthropods), and tetrapods(temnospondyl amphibians, seymouriamorphs, cotylosaurs, synapsids, diapsids), as well as bony fishes. General characteristics of the taxonomical composition of the Kuedinskie Kluchiki locality are given. A new peltaspermalean pteridosperm taxon, Compsopteris longipinnata sp. nov., and a voltzialean conifer Archaeovoltzia kuedensis sp. nov. are described. General considerations on the paleoecologic and paleogeographic conditions of the Kuedinskie Kluchiki locality origin are given.

Shells in the cabinet:four new species of Tellinidae(Mollusca:Bivalvia:Tellinoidea)identified from existing South China Sea collections

Four species new to science were identified in the Tellinidae museum collections in the Marine Biological Museum of Chinese Academy of Sciences.Pinguitellina triquetra sp.nov.,collected in 1981,has not previously been described or reported.It differs from other Pinguitellina species by its trigonal shape,rose-colored valve interior,and deep and wide pallial sinus,which is confluent with the pallial line.Pinguitellina protuberangla sp.nov.has a broad and deep dorsal corner,short and straight posterodorsal margin,comparatively large adductor scars,depressed and fragile shells,and deep socket at the anterior edge of the right valve.Pinguitellina minuta sp.nov.was previously misidentified as Arcopella casta in China.It is a new species based on its unique characteristics,which include small,fragile,inflated,translucent,and triangularly ovate-shaped shells with acuminated and convex umboes.As the first species described in China within the genus Abranda Iredale,1924,Abranda xui sp.nov.has internal ligaments and thin,fragile,and translucent elliptical shells with fine radials on the surface.In this study,we reorganized the species currently contained in the two genera Pinguitellina and Abranda and created keys to the known species.We believe that numerous collected bivalve specimens have been overlooked in the South China Sea,and to date,mollusk has not been adequately sampled.Thus,further exploration and analysis are required to understand species diversity in this area.

Lineaperpetua gen.nov.:a new diatom genus in the Thalassiosirales supported by morphology and molecular data

Based on a combination of morphology and molecular data of ribosomal DNA genes,a new diatom genus Lineaperpetua gen.nov.Yu,You,Kociolek&Wang is described.The features that help define Lineaperpetua at the level of genus include:a tangentially undulated valve face;continuous cribra areolae on the valve interior consisting of pores arranged as strips;single rimoportula located inside the ring of marginal fultoportulae.Additionally,phylogenetic analysis based on nuclear small subunit(SSU)rDNA sequences and nuclear large subunit(LSU)rDNA gene placed the three strains of L.lacustris in a single,monophyletic clade at a considerable sequence distance from the other genera(Thalassiosira,Conticribra,Planktoniella,Shinodiscus,and other genera)belonging to Thalassiosirales.Despite the similarities with some species of Thalassiosira,Conticribra,and Spicaticribra,the suite of features found in Lineaperpetua differentiate it from these other genera.These molecular data and morphological characters suggest an affinity of the new genus to the Thalassiosiraceae.

Neomicrocoleus gen.nov.(Microcoleaceae,Oscillatoriales),a novel cyanobacterial genus from benthic mats in a water channel

A new filamentous cyanobacterial strain(CHAB 4127)was successfully isolated from the algal mat during the field investigation of the open channel from Luanhe River to Tianjin,China.The polyphasic approach combining morphological,ultrastructural,ecological,and molecular features was used to characterize this studied strain.The strain is morphologically similar to the Microcoleus-like cyanobacterial taxa under light microscopy,and the radial arrangement of thylakoids is also consistent with that of Microcoleus-like groups.The phylogenetic position of CHAB 4127 based on 16S rRNA gene sequences,is shown to be clearly clustered into an independent clade with the newly established genus Microcoleusiopsis.The maximum similarity of 16S r RNA gene of the studied strain with other existing related cyanobacterial taxa is 93.97%,and the ITS secondary structures is also obviously different from other members of Microcoleaceae.Based on all the above evidence,we proposed the establishment of this novel cyanobacterial genus as Neomicrocoleus,with its type species,Neomicrocoleus tianjinensis.

Cretaceous crown male ant reveals the rise of modern lineages

Most described Mesozoic ants belong to stem groups that existed only during the Cretaceous period.Previously,the earliest known crown ants were dated to the Turonian(Late Cretaceous,ca.94-90 million years ago(Ma))deposits found in the USA,Kazakhstan,and Botswana.However,the recent discovery of an alate male ant in Kachin amber from the earliest Cenomanian(ca.99 Ma),representing a new genus and species,Antiquiformica alata,revises the narrative on ant diversification.Antiquiformica can be distinctly differentiated from all known male stem ants by its geniculate antennae with elongated scape,extending far beyond the occipital margin of the head and half the length of the funiculus,as well as its partly reduced forewing venation.Furthermore,the combination of a one-segmented waist with a well-developed node,elongated scape extending beyond the occipital margin,and reduced forewing venation,particularly the completely reduced m-cu and rs-m crossveins and absence of rm and mcu closed cells,firmly places the fossil within the extant subfamily Formicinae.Fourier transform infrared spectroscopy(FTIR)confirmed that the amber containing Antiquiformica alata originated from the Kachin mines in Myanmar.This discovery significantly revises our understanding of the early evolution of Formicinae.The presence of Antiquiformica in Cenomanian amber indicates that the subfamily Formicinae emerged at least by the start of the Late Cretaceous,with crown ants likely originating earlier during the earliest Cretaceous or possibly the Late Jurassic,although paleontological evidence is lacking to support the latter hypothesis.

New record of the feather star Palaeocomatella hiwia(Echinodermata:Crinoidea:Comatulida)from Zhenbei Seamount in South China Sea:new insights into the taxonomic position of Palaeocomatella

One specimen belonging to the family Comatellinae was collected from the Zhenbei Seamount(332.5–478.2 m)in the South China Sea in July 2022.Based on the morphological characters,the specimen was identified as Palaeocomatella hiwia McKnight,1977.It is first recorded from China Sea and redescribed in detail.This specimen differs from the original description from New Zealand for never showing syzygy at br4+5 or br5+6 on interior and br1+2 on exterior arms.However,it is much conform to the redescription to specimens from Indonesia,with only differences in position of the second syzygy and distalmost pinnule comb.Specimen is deposited in the Institute of Oceanology,Chinese Academy of Sciences.Phylogenetic analyses based on the mitochondrial c oxidase subunit I(COI)and 16S rRNA genes indicated that P.hiwia was nested within the tribe Phanogeniini and clustered with Aphanocomaster pulcher.Furthermore,P.hiwia showed same morphological features in terms of mouth placement,comb location,and number of comb teeth rows as other genera of Phanogeniini.Therefore,we suggest that the genus Palaeocomatella should be put in the tribe Phanogeniini.

Yuzaoea gen. nov., a new biraphid diatom(Bacillariophyceae)genus and its phylogenetic significance

The flexed frustules in pennate diatoms are usually associated with monoraphid diatoms. Interestingly, we found a biraphid diatom species with flexed frustules in an offshore intertidal beach environment on Weizhou Island,Beihai City, Guangxi Zhuang Autonomous Region, China. Therefore, based on morphological characteristics, we described a new genus of diatoms Yuzaoea sinensis gen. et sp. nov. CH Li, HH Liu, YH Gao & CP Chen. The frustule of this genus is characterized by heterogeneous frustule with one concave valve and one convex valve,complete raphe on both valves, straight and moderately eccentric raphe, uniseriate striae and girdle bands with a single row of areolae. The most identifying feature of this genus was the flexed frustule, which is rare in biraphid diatoms and common in monoraphid diatoms. We compared the morphometric characteristics of genus Yuzaoea with genus Rhoikoneis and several genera within the family Rhoicospheniaceae, including Rhoicosphenia,Campylopyxis, and Cuneolus. Phylogenetic analyses based on SSU rRNA and rbc L showed that the genus Yuzaoea was the sister group to the clade of Rhoicosphenia with a high support value(bootstrap values = 100%), and the clade “Yuzaoea+Rhoicosphenia” was sister to the clade of monoraphid diatoms, in which the genera Achnanthidium, Planothidium and some Cocconeis with high support values(bootstrap = 100%).Morphologically, the genus Yuzaoea shares many morphological features with monoraphid diatoms like genera Achnanthidium and Planothidium and the members within the Rhoicospheniaceae. Therefore, based on a combined morphological studies and phylogenetic results we suggested that this branch may represented the evolution of one kind monoraphid diatoms, from biraphid diatoms(e.g. genus Yuzaoea), to incompleted biraphid diatoms(e.g. genera Rhoicosphenia, Campylopyxis), to monoraphid diatoms(e.g. genera Achnanthidium and Planothidium).