Tea saponins affect in vitro fermentation and methanogenesis in faunated and defaunated rumen fluid

The effect of tea saponins (TS) on rumen fermentation and methane emission was examined using an in vitro gas production technique named Reading Pressure Technique. Three levels of TS addition (0, 0.2, 0.4 mg/ml) were evaluated in the faunated and defaunated rumen fluid. Compared to the control, TS addition decreased the 24 h gas production in the faunated rumen fluid, but had a minor effect on gas yield in the defaunated rumen fluid. The TS significantly reduced methane production in vitro. In the faunated rumen fluid, 0.2 or 0.4 mg/ml TS decreased the 24 h methane emission by 12.7% or 14.0%, respectively. Rumen fluid pH value was affected neither by TS addition nor by defaunation. The TS addition had only minor effects on volatile fatty acids, but the yield and pattern of volatile fatty acids were greatly affected by defaunation. While the molar proportion of acetate was not affected by defaunation, the propionate was significantly increased and the butyrate significantly decreased. Ammonia-N concentration and microbial protein yield were influenced by TS inclusion and defaunation. Inclusion of 0.4 mg/ml TS increased the microbial protein mass by 18.4% and 13.8% and decreased the ammonia-N concentration by 8.3% and 19.6% in the faunated and defaunated rumen fluid, respectively. Protozoa counts were significantly reduced by TS inclusion. The current study demonstrated the beneficial effect of TS on methane production and rumen fermentation, and indicated that this may be due to the effect of the associated depression on protozoa counts.

The community structure and microbial linkage of rumen protozoa and methanogens in response to the addition of tea seed saponins in the diet of beef cattle

Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 emission.Conclusions: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.

Study on Extraction of Tea Saponin from Camellia oleifera Cake Using Water as Extraction Solvent

This paper studied the effects of liquid-solid ratio, temperature, time and pH value on the extraction rate of tea saponin from the cake of Camellia oleifera seeds by using single factor experiment with the cake of Camellia oleifera seeds as the raw materials, and water as the extraction solvent, and orthogonal test was used to determine the optimal extraction process conditions. The results showed that the extraction ratio of tea saponin could reach up to 95.50% when the liquidsolid ratio was 11：1, extracting temperature of 80 ℃, extraction time of 6 h, and pH value of 9.

Preparation and Dye Adsorption of Low-cost Polyaniline-tea Saponin Nanocomposites

Four polyaniline-tea saponin (PTS) nanocomposites were prepared by an in-situ polymerization with tea saponin (TS) as a biosurfactant,and they were used to remove organic dyes from aqueous solution.The PTS nanocomposites were characterized by using field emission scanning electron microscopy,the Fourier transform infrared spectroscopy,the Ultraviolet-visible spectroscopy,and the thermogravimetric analysis.The adsorption performances of the PTS nanocomposites for organic dyes were studied by a static adsorption method.The experimental results reveal that adsorption capacities of the PTS nanocomposites are higher than that of pure polyaniline.Especially,the PTS nanocomposites exhibit excellent adsorption performances for anionic dyes because of the electrostatic interaction between the positively charged nitrogen atoms on the PTS chains and the negatively charged sulfonate ions in the anionic dyes.According to the adsorption kinetics and thermodynamics results,the adsorption processes of PTS20 for CR and AB74 follow well with the pseudo second-order and Langmuir isotherm models.It is indicated that TS should be very useful in the preparation of PTS nanocomposite and in removal of toxic dyes from waste water.

Efficient Extraction of Tea Saponin from Tea Seed Meals by ^(60)Coγ-Irradiation

In order to simplify the extraction process of tea saponin and improve its extraction rate,tea seed meals was taken as materials to explore effects of ^(60)Coγ-irradiation and H_(2)O_(2) on the extraction rate of tea saponin.The results showed that the optimal crude extract condition of tea saponin was 6%H_(2)O_(2) and an irradiation dose of 5 kGy,the extraction rate was 46.4%.However,when the irradiation dose and the H_(2)O_(2) concentration continued to increase,the extraction rate of tea saponin decreased.After flocculate extraction,the tea saponin content increased from 40.80%to 48.32%.Foaming property test results showed that the higher the tea saponin content in the solution was,the higher the foaming height will be,and the foam had a certain stability.This work provided a new method for the extraction of natural products,usingγ-ray irradiation and H_(2)O_(2) pretreatment to improve the extraction rate,and this was of great significance for industrial extraction.

Evaluation of Effects of Tea Saponin on Behavior, Growth and Development of Cylas formicarius (Coleoptera: Brentidae)

[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] The olfactory avoidance rate of C. formicarius to tea saponin aqueous solution was determined by Y-type olfactometer;the feeding avoidance rate of C. formicarius to tea saponin was determined by the selective method;the antifeedant rate of C. formicarius to tea saponin was determined by non-selective method;and the development duration and mortality of C. formicarius under the influence of tea saponin were determined by artificial feeding method. [Results] C. formicarius had no significant olfactory tendency to every concentration of tea saponin, and the olfactory avoidance rate of 20.0% tea saponin aqueous solution was only 9.14%. Tea saponin had a feeding avoidance effect on C. formicarius, and the avoidance rate increased with the increase of tea saponin concentration. At 6 h, the feeding avoidance rates of 0.5%, 1.0%, 5.0%, 10.0% and 20.0% tea saponin on C. formicarius were 58.14%, 77.77%, 88.23%, 95.00% and 97.65 %, respectively;and the feeding avoidance effect at 6 h was significant, and the feeding avoidance rate was higher than that of 1 h. Tea saponin had a significant antifeedant effect on C. formicarius, and the longer the feeding time, the higher the antifeedant rate. At 72 h, the antifeedant rates of 0.5%, 1.0%, 5.0%, 10.0 % and 20.0% tea saponin to C. formicarius were 63.01%, 67.54%, 97.14 %, 96.42% and 98.57%, respectively. The larval development duration of C. formicarius was shortened with the increase of tea saponin concentration, and the larval death occurred. The development duration of larvae under the influence of 1.0% tea saponin was the shortest, which was 4.01 d shorter than that of the control, and the mortality was the highest, which was 26.65%. [Conclusions] Tea saponin had neither olfactory avoidance effect nor olfactory attracting effect to C. formicarius, but had obvious feeding avoidance effect and strong antifeedant effect. Tea saponin can shorten the development duration of the larvae of C. formicarius and cause the death of the larvae.

Quantitative analysis and screening for key genes related to tea saponin in Camellia Oleifera Abel.Seeds

Tea saponin is an important factor affecting the taste of oil-tea camellia seed oil.The content of tea saponin varied in different plant parts and different oil-tea camellia varieties.The tea saponin content was the highest in oil-tea camellia seed oil of Liping 2,3.392 mg/mL,and the lowest in that from Changlin 53,1.016 mg/mL.With increased storage time,the content of tea saponin first increased,peaked at 21 days,and then decreased.Transcriptome sequencing was performed to identify genes related to Camellia Oleifera tea saponin metabolism.A total of 8107 differentially expressed genes were identified,and these genes were classified into 49 Gene Ontology(GO)functional categories and involved 129 Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways.Genes highly correlated with tea saponin metabolism showed significant variation,including c96515.graph_c0(annotated as acetyl-CoA C-acetyltransferase,AACT),c92925.graph_c0(annotated as hydroxymethyl glutaryl-CoA synthase,HMGS),c95088.graph_c4,c92367.graph_c0(annotated as hydroxymethyl glutaryl-CoA reductase,HMGR),c100030.graph_c3(annotated as 1-deoxy-D-xylulose-5-phosphate synthase,DXS),c86734.graph_c0,c108153.graph_c1(annotated asβ-amyrin synthase),c92020.graph_c0,c105492.graph_c1,c92290.graph_c1(annotated as cytochrome P450,CYP450),c102136.graph_c0,c95951.graph_c1(annotated as UDP-Glycosyltransferase,UGT).This research provides theoretical reference for improved processing and utilization of Camellia Oleifera.