Tea (Camellia sinensis L.) seed shells, the main byproduct of the manufacture of tea seed oil, were used as precursors for the preparation of tea activated carbon (TAC) in the present study. A high yield (44.1%) of TA...Tea (Camellia sinensis L.) seed shells, the main byproduct of the manufacture of tea seed oil, were used as precursors for the preparation of tea activated carbon (TAC) in the present study. A high yield (44.1%) of TAC was obtained from tea seed shells via a one-step chemical method using ZnCl2 as an agent. The Brunauer-Emmett-Teller (BET) surface area and the total pore volumes of the obtained TAC were found to be 1 530.67 mg2/g and 0.7826 cm3/g, respectively. The equilibrium adsorption results were complied with Langmuir isotherm model and its maximum monolayer adsorption capacity was 324.7 mg/g for methylene blue. Adsorption kinetics studies indicated that the pseudosecond-order model yielded the best fit for the kinetic data. An intraparticle diffusion model suggested that the intraparticle diffusion was not the only rate-controlling step. Thermodynamics studies revealed the spontaneous and exothermic nature of the sorption process. These results indicate that tea seed shells could be utilized as a renewable resource to develop activated carbon which is a potential adsorbent for methylene blue.展开更多
利用固定化细胞发酵技术,选取黑曲霉、枯草芽孢杆菌和酿酒酵母对茶籽壳进行发酵,制备发酵物。分析显示:与未经发酵的水提取物相比,茶籽壳酿酒酵母发酵物(FETSS-3)主要抗氧化活性物质含量明显增加,其多糖、多酚和皂苷含量分别增加至原来...利用固定化细胞发酵技术,选取黑曲霉、枯草芽孢杆菌和酿酒酵母对茶籽壳进行发酵,制备发酵物。分析显示:与未经发酵的水提取物相比,茶籽壳酿酒酵母发酵物(FETSS-3)主要抗氧化活性物质含量明显增加,其多糖、多酚和皂苷含量分别增加至原来的 9.19 、9.59 和 4.67 倍。从生化水平、细胞水平和酶活性水平对其抗氧化活性进行了探究,并对其皮肤安全性进行试验确认。研究结果表明:在 0.1~ 0.4 g/L和0.5~2.0 g/L,FETSS-3的ABTS和· OH自由基清除活性显著优于V C ( P <0.01;P <0.01);在0.2~1.2 g/L,其对脂质过氧化半抑制率IC 50 仅为V E 的21.98%,抑制活性显著高于V E ( P <0.01)。FETSS-3在20~120 mg/L能显著降低H 2 O 2 诱导的人皮肤成纤维细胞(HSF)内的氧化损伤应激水平( P <0.01)。体外酶活性实验表明:FETSS-3(120 mg/L)能显著提高HSF细胞氧化应激损伤后的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和人谷胱甘肽-过氧化物酶(GSH-PX)活性( P <0.05;P <0.01;P <0.01)。人体斑贴实验表明:FETSS-3无皮肤过敏风险。展开更多
基金supported by the Science and Technology Department of Zhejiang Province (No. 2010C32051)the Ministry of Science and Technology (No. 2011BAD01B03-5-1), China
文摘Tea (Camellia sinensis L.) seed shells, the main byproduct of the manufacture of tea seed oil, were used as precursors for the preparation of tea activated carbon (TAC) in the present study. A high yield (44.1%) of TAC was obtained from tea seed shells via a one-step chemical method using ZnCl2 as an agent. The Brunauer-Emmett-Teller (BET) surface area and the total pore volumes of the obtained TAC were found to be 1 530.67 mg2/g and 0.7826 cm3/g, respectively. The equilibrium adsorption results were complied with Langmuir isotherm model and its maximum monolayer adsorption capacity was 324.7 mg/g for methylene blue. Adsorption kinetics studies indicated that the pseudosecond-order model yielded the best fit for the kinetic data. An intraparticle diffusion model suggested that the intraparticle diffusion was not the only rate-controlling step. Thermodynamics studies revealed the spontaneous and exothermic nature of the sorption process. These results indicate that tea seed shells could be utilized as a renewable resource to develop activated carbon which is a potential adsorbent for methylene blue.
文摘利用固定化细胞发酵技术,选取黑曲霉、枯草芽孢杆菌和酿酒酵母对茶籽壳进行发酵,制备发酵物。分析显示:与未经发酵的水提取物相比,茶籽壳酿酒酵母发酵物(FETSS-3)主要抗氧化活性物质含量明显增加,其多糖、多酚和皂苷含量分别增加至原来的 9.19 、9.59 和 4.67 倍。从生化水平、细胞水平和酶活性水平对其抗氧化活性进行了探究,并对其皮肤安全性进行试验确认。研究结果表明:在 0.1~ 0.4 g/L和0.5~2.0 g/L,FETSS-3的ABTS和· OH自由基清除活性显著优于V C ( P <0.01;P <0.01);在0.2~1.2 g/L,其对脂质过氧化半抑制率IC 50 仅为V E 的21.98%,抑制活性显著高于V E ( P <0.01)。FETSS-3在20~120 mg/L能显著降低H 2 O 2 诱导的人皮肤成纤维细胞(HSF)内的氧化损伤应激水平( P <0.01)。体外酶活性实验表明:FETSS-3(120 mg/L)能显著提高HSF细胞氧化应激损伤后的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和人谷胱甘肽-过氧化物酶(GSH-PX)活性( P <0.05;P <0.01;P <0.01)。人体斑贴实验表明:FETSS-3无皮肤过敏风险。