经皮穴位电刺激(TEAS)特定穴联合补肾益智活血汤对急性脑损伤患者认知功能、血清神经损伤标志物的影响

【目的】分析经皮穴位电刺激(TEAS)特定穴联合补肾益智活血汤对急性脑损伤患者认知功能、血清神经损伤标志物的影响。【方法】通过便利抽样法,回顾性收集100例急性脑损伤患者,根据治疗方法的不同分为观察组和对照组,每组各50例。对照组给予常规西医治疗,观察组在对照组的基础上给予TEAS特定穴联合补肾益智活血汤治疗,疗程为3周。观察2组患者治疗前和治疗1周、2周、3周后格拉斯哥昏迷指数(GCS)评分、美国国立卫生研究院卒中量表(NIHSS)评分、蒙特利尔认知评估量表(MoCA)评分、血清神经损伤标志物水平及颅内动脉血流速度的变化情况,比较2组患者的临床疗效、脑血管痉挛(CVS)和不良反应发生率。【结果】(1)治疗3周后,观察组的总有效率为96.00%(48/50),对照组为76.00%(38/50),组间比较,观察组的疗效明显优于对照组(P<0.01)。(2)观察组治疗1周、2周、3周后和对照组治疗2周、3周后的GCS评分、MoCA评分均较治疗前升高(P<0.05),NIHSS评分均较治疗前降低(P<0.05),且观察组在治疗1周、2周、3周后对GCS评分、MoCA评分的升高幅度及对NIHSS评分的降低幅度均明显优于对照组(P<0.05或P<0.01)。(3)观察组治疗1周、2周、3周后和对照组治疗2周、3周后的血清神经元特异性烯醇化酶(NSE)、神经肽Y(NPY)水平均较治疗前降低(P<0.05),且观察组在治疗1周、2周、3周后对血清NSE、NPY水平的降低幅度均明显优于对照组(P<0.05或P<0.01)。(4)观察组治疗1周、2周、3周后和对照组治疗2周、3周后的椎动脉(VA)、基底动脉(BA)、大脑中动脉(MCV)血流速度均较治疗前升高(P<0.05),且观察组在治疗1周、2周、3周后对VA、BA、MCV血流速度的升高幅度均明显优于对照组(P<0.05或P<0.01)。(5)观察组的CVS发生率为2.00%(1/50),明显低于对照组的16.00%(8/50),差异有统计学意义(P<0.05)。(6)观察组的不良反应发生率为4.00%(2/50),对照组为8.00%(4/50),组间比较,差异无统计学意义(P>0.05)。【结论】TEAS特定穴联合补肾益智活血汤可有效改善急性脑损伤患者神经功能和认知功能,减轻脑损伤与昏迷程度,降低CVS发生率,且联合治疗并未增加不良反应,具有一定的安全性。

不同经穴TEAS对低血压大鼠痛阈及血压调节效应的实验研究

目的:观察不同经穴经皮穴位电刺激(transcutaneous electrical acupoint stimulation,TEAS)对低血压大鼠痛阈及血压的调节效应。方法:将50只低血压模型大鼠随机分成足三里、三阴交、太冲、曲池和合谷穴5个组,每组10只,采用经皮穴位电刺激法介入治疗,刺激参数为疏密波(频率2/100Hz)、强度(4±1)mA,时间为40 min,每天治疗1次,连续治疗3 d。采用辐射热甩尾法测量造模后、治疗中及治疗后不同时段痛阈。采用无创动物血压观察系统BP-5测量TEAS治疗前、治疗疗程结束后即时、治疗后不同时段的大鼠尾动脉收缩压。结果:5个经穴组经皮电刺激后均能不同程度提高大鼠痛阈,治疗后与自身痛阈相比具有统计学差异(P<0.05),除合谷穴外,4个经穴组经皮电刺激后均能不同程度提高低血压大鼠血压,治疗后与治疗前血压相比具有统计学差异(P<0.05)。结论:足三里、三阴交、太冲、曲池、合谷穴均能提高低血压大鼠痛阈,以足三里穴提高幅度最明显,具有较好的镇痛效应,同时,足三里、三阴交、太冲、曲池穴均能提高低血压大鼠血压,且以足三里穴升压效应最为明显。

Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Tea is a widely consumed beverage and has many important physiological properties and potential health benefits. In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was fully validated and successfully applied to the determination of these amino acids in six different tea samples. Good linearity (r ≥ 0.993), precision (RSDs≤ 2.99%), accuracy (91.95%-107.09%) as well as good sample stability were observed. The limits of detection ranged from 1.42 to 14.69 ng/mL, while the limits of quantification were between 4.53 and 47.0 ng/mL. The results indicate that the contents of the 11 amino acids in the six different tea samples are greatly influenced by the degree of fermentation. The proposed SFC-MS method shows a great potential for further investigation of tea varieties.

技术经济分析系统《TEAS》的设计与实现

研究工程造价计算和技术经济分析领域的应用软件系统如何实现开放的、通用化、工具化的设计是一项困难而又有实际意义的课题。本文提出的采用面向领域语言的设计模型解决了这一难题。文中从设计角度讨论了《ＴＥＡＳ》的设计思想、关键技术，较详细地介绍了作者在系统中定义的一种面向技经领域的模式描述语言ＭＤＬ。

Discursive Constraints of Teasing:Constructing Professionality via Teasing in Chinese Entertainment Interviews

Teasing can be approached as a linguistic resource for examining the interpersonal issues of im/politeness and face,or as a discursive strategy for displaying relationships or constructing social identities.However,studies have underestimated the discursive constraints of teasing in specific contexts.Meanwhile,a majority of teasing studies were based on Western cultures and did not pay sufficient attention to the variety of teasing across cultures.By collecting data from two Chinese entertainment interviews,where the interviewer employs teasing frequently for performing institutional roles,this study examined how teasing functions to assist the interviewer to complete communicative goals,and explored the discursive constraints of teasing in media context.Data analysis exemplified how teasing helped the interviewer to manage an interview event,obtain the guest’s disclosure and seek audience involvement,helping to construct the interviewer’s professionality.Implications for understanding the discursive features of teasing in the Chinese media context were addressed.

Determination of Trace Elements in Famous Teas from Huangshan Area by ICP-AES

With an Iris Intrepid II XSP ICP full spectrum ICP emission spectrometer,trace elements including Se,Fe,Mn,Cd,Cr,Co,Ni,Cu,Pb and Zn in Huangshan teas were determined.The best analysis conditions were selected by experiments.The method is simple and rapid,and the precision and accuracy are all in line with the requirements.This method was used to analyze actual tea samples with satisfactory results.

Investigation of SARS-CoV-2 Main Protease Potential Inhibitory Activities of Some Natural Antiviral Compounds Via Molecular Docking and Dynamics Approaches

Coronaviruses caused an outbreak pandemic disease characterized by a severe acute respiratory distress syndrome leading to the infection of more than 200 million patients and the death of more than 4 million individuals.The primary treatment is either supportive or symptomatic.Natural products have an important role in the development of various drugs.Thus,screening of natural compounds with reported antiviral activities can lead to the discovery of potential inhibitory entities against coronaviruses.In the current study,an in-silico molecular docking experiment was conducted on the effects of some of these natural antiviral phytoconstituents,(e.g.,procyanidin B2,theaflavin,quercetin,ellagic acid,caffeoylquinic acid derivatives,berginin,eudesm-1β,6α,11-triol and arbutin),on the crystal structure of SARS-CoV-2 main protease(PDB ID:6w63)using AutoDock-Vina software.Many of the docked compounds revealed good binding affinity,with procyanidin B2(–8.6 Kcal/mol)and theaflavin(–8.5 Kcal/mol)showing a better or similar binding score as the ligand(–8.5 Kcal/mol).Molecular dynamics simulations were carried out at 100 ns and revealed that procyanidin B2 forms a more stable complex with SARS-CoV-2 main protease than theaflavin.Procyanidin B2,theaflavin,and 4,5-dicaffeoylquinic acid were evaluated for toxicity by ProTox-II webserver and were non-toxic according to the predicted LD50 values and safe on different organs and pathways.Additionally,these phytoconstituents showed good ADME properties and acceptable lipophilicity,as evaluated using WLOGP.Amongst the tested compounds,procyanidin B2 showed the highest lipophilic value.It is worth mentioning that these natural inhibitiors of SARS-CoV-2 main protease are components of green and black tea that can be used as a supporting supplement for COVID patients or as potential nuclei for further drug design and development campaigns.

Field Evaluation of Non-aerated Sheep Manure and Water Jacinthe(Eichhornia crassipes)Composts Teas Effects on Tomato(Solanum lycopersicum L.)Health,Mycorrhization and Yield in Mali

This study was conducted in context to preserve tomato healthiness and to improve its yield in field.It aimed to assess some effects of non-aerated sheep manure and water jacinthe(Eichhornia crassipes)composts teas.It specifically consisted to evaluate two teas effects on tomato’s:(a)A.solani foliar disease,(b)yield and(c)mycorrhization intensity.The experiments were conducted according to completely randomized Fisher block with four repetitions.Three treatments were considered:plants receiving non-aerated sheep manure compost tea(TCFM),plants receiving non-aerated water jacinthe compost tea(TCJE),and control plants(T0)without compost tea application.The experiments were repeated twice.Teas application started at four-leaf stage of tomato plants.It was weekly and insured by manual foliar spraying and watering of the plants.To determine the preventive suppressive effects of compost teas,three days after its initial application,plants were artificially inoculated by foliar spraying with Alternaria solani sporal suspension.Data were analyzed with R software.Tukey Contrasts test at 5%threshold was used to compare means.Results showed that TCFM was the most suppressive on A.solani and also had enhanced tomato yield in field.Both teas did not have a meaningful effect on tomato mycorrhization intensity.These results showed that TCFM can serve as biopesticide and biofertilizer in tomato culture.

TEAS复合全麻对妇科腹腔镜患者术后认知功能的影响

评估妇科腹腔镜患者,在术中使用TEAS复合全麻对于认知功能的干预情况。方法:利用随机数字表法将我院妇科(研究时间:2018年10月~2020年10月)接诊的60例腹腔镜治疗的患者分为两个不同的小组,并给予不同的麻醉处理形式。给予全麻处理的小组命名为对照组(n=30),给予TEAS复合全麻的小组命名为观察组(n=30),将两组妇科手术患者恢复情况进行比较。结果:术后1天,与对照组NSE、S-100β相比,观察组NSE(8.52±1.05)μg/L和S-100β(0.175±0.005)μg/L更小,P<0.05;与对照组MMSE评分相比,观察组术后术后1天(27.85±0.51)分和术后3天MMSE评分(27.86±0.66)分更高,P<0.05;与对照组认知功能障碍相比,观察组(3.33%)更低,P<0.05;与对照组血液流变学指标相比,观察组术后全血高切黏度(6.03±0.22)mPa·s、全血低切黏度(7.25±1.21)mPa·s、血浆黏度(1.38±0.13)mPa·s、血细胞比容(41.33±0.36)%及血小板黏附率(26.52±6.58)%更高,P<0.05。结论:妇科腹腔镜患者手术中应用TEAS复合全麻效果显著,具有推广意义。

Discrimination of Green,Oolong,and Black Teas by GC-MS Analysis of Characteristic Volatile Flavor Compounds

Tea is one of the most consumed beverages in the world and its quality is influenced by geographical origin and production methods. This study focuses on the volatile aroma components of 38 tea products from China, Japan, Indonesia, Sri-Lanka, and Chinese Taipei;among them 7 green teas, 13 oolong teas, and 18 black teas. The volatiles were extracted from the infusions using PorapakQ-resin, concentrated, and analyzed by gas chromatography-mass spectrometry. The components were identified by authentic reference compounds or preliminary based on their mass spectra. Different manufacturing processes yield different blends of aroma compounds. In general, the contents of total volatiles, aliphatics, aromatics, and terpenoids increased with the fermentation degree, whereas jasmine lactone and indole were the highest in oolong teas. Some particular manufacturing processes, for example, the use of tea leaves infested by the tea green leafhopper, lead to higher contents of volatiles in final products as in Oriental Beauty oolong tea. The relative peak areas determined for 82 volatiles were the basis for the statistical analysis and highlight the potential of multivariate analysis to distinguish tea samples of different categories.

Determination of the total antioxidant and oxidant status of some galactagogue and herbal teas

This research was aimed to evaluate the total antioxidant status(TAS)and the total oxidant status(TOS)of galactagogues and herbal teas.Galactagogues and herbal teas used for other purposes were sold in the market for breastfeeding mothers and babies.Tea infusions were prepared by following brewing recipe on the package.TAS(mmol/L)and TOS(mol/L)were measured and oxidative stress indices(OSI)were calculated.TAS values of the mixed-herbal teas in granule form that sold as still tea were found to be higher((1.5±0.00)–(1.7±0.14)mmol/L)compared to other galactagogue tea types(P<0.05).TAS value of the mixed-herbal tea in classic tea bag was(1.0±0.06)mmol/L while the TAS value of the vitamin added mixed-herbal tea was(3.0±0.00)mmol/L(P<0.05).The TAS and TOS values of the night-teas were simultaneously found to be higher compared to herbal teas(P<0.05).Besides,the highest OSI values among herbal teas was chamomile tea(6.96).In conclusion,antioxidant/oxidant status of galactagogues and other herbal teas might vary according to their form(powder or granule),type,variety of herbs in the composition,and the brand.Moreover,some galactagogues and other herbal teas have high levels of oxidant activity as well as antioxidant activity.Also,it seems that vitamin addition is important for balancing antioxidant/oxidant status of all tea types.

Chemopreventive Potential of Select Herbal Teas and Spices on Azoxymethane-Induced Aberrant Crypt Foci in Fisher 344 Male Rats

Colon cancer is the third leading cause of death in the US. Selected herbal teas and spices may reduce incidence of chronic diseases, including cancer. The objective of this study was to identify the effect of strawberry leaf, raspberry leaf, hibiscus teas and cinnamon on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in Fisher 344 male rats. After acclimatization period (1 wk), 49 male weanling rats were divided into 16 groups. Control (CON) group fed AIN-93G diet;15 treatment groups were administered control diet + strawberry leaf tea (STW), raspberry leaf tea (RAS), hibiscus tea (HIB), cinnamon (CIN), strawberry leaf tea + cinnamon (STW + CIN), raspberry leaf tea + cinnamon, (RAS + CIN), hibiscus tea + cinnamon (HIB + CIN), and strawberry leaf tea + raspberry leaf tea + hibiscus tea + cinnamon in combination (COM) at 2 levels each (teas added at 1% and 2%;CIN added at 2.5% and 5%). Rats received 24 mg/kg body weight AOM in saline s/c at 7 and 8 weeks of age. Animals received experimental diets until sacrificed by CO2 asphyxiation (17 weeks of age). ACF were enumerated in colons. Hepatic antioxidant enzymes were determined;superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione (GH). Treatment groups had reduction in ACF compared to CON (154). Lowest ACF observed in HIB 2% + CIN 5% (13.16) with 91.45% reduction compared to CON. ACF observed in treatment groups administered teas and cinnamon combinations were lower than those administered teas singly. SOD and CAT activities in rats administered treatment diets were higher than CON (13.63 U/mL, 0.95 umol·min-l·ml-1). Rats administered COM (20.65 U/mL) had highest SOD activity. CAT activity was 51.27% higher in rats administered HIB 2% (1.96 umol·min-l·ml-1). GPX activity ranged from 7.26 (STW 1% + CIN 2.5%) to 9.59 (STW 2%) umol·min-l·ml-1. Results suggest that herbal teas and spices may reduce the risk of colon cancer and improve antioxidant status;regular consumption may provide beneficial health effects.

Health-Benefit Assets of Different Indian Processed Teas: A Comparative Approach

A comparative deliberation has been considered among three Indian processed teas (black, green and white) in respect to their prevalence of some secondary metabolites, antioxidant ability (ABTS and DPPH assay), nutritional properties, inorganic elemental profile and bactericidal efficiency. Green and white tea, incidence of total phenol, total flavonoids, proanthocyanidins and tannin are higher than the black. ABTS and DPPH study reveals lower IC50 occurred in Green tea. Green tea is enriched in Na, Fe, Mg, and Mn content;black is rich in K, Ca and white has highest Zn. Total sugar and free amino acid are highest in white tea;total protein content is almost same in all three types. Green tea is enriched with vitamin C. Antimicrobial asset is experienced against Bacillus subtilis, Staphylococcus aureus (gram + ve) and Escherichia coli and Pseudomonas aeruginosa (gram?-?ve) bacteria. Study revealed that green tea has higher antimicrobial activity than the other two, though higher inhibitory effect of black tea might be attributed to the presence of substantial amount of tannin. Statistical evaluation reflects that in all organic and aqueous extracts, secondary metabolites correlate linearly with DPPH and ABTS assays but are not consistent with bactericidal efficiency in all cases.

Chemopreventive and Anti-Inflammatory Potential of Select Herbal Teas and Cinnamon in an <i>In-Vitro</i>Cell Model

Colon cancer is the third leading cause of death in the US. Herbal teas and spices may reduce the incidence of chronic diseases, including colon cancer. The objectives of this study were to determine the chemopreventive effects of herbal teas and cinnamon in an in-vitro cell model and to evaluate the inhibitory effects of selected extracts on enzymes associated with inflammatory disease. Effects of raspberry leaf (0.5 - 2.0 mg/mL), strawberry leaf (0.4 - 1.0 mg/mL), hibiscus flower (4.0 - 10.0 mg/mL) and cinnamon (400 - 1500 μg/mL) were evaluated for cytotoxicity, induction of caspase and DNA fragmentation in colon cancer (Caco-2) cells to determine possible chemopreventive effects. Effects of extracts on inhibition of cyclooxygenase-2 (COX-2) were also measured to determine possible anti-inflammatory potential. Caco-2 cells were obtained from American Type Culture Collection (ATCC) and maintained in Dulbecco’s Modified Eagle’s Medium with 10% fetal bovine serum. As concentrations of tea increased, LDH release from Caco-2 cells increased, with cytotoxicity ranging from 1% - 80% (hibiscus flower 1.0 mg/mL and strawberry leaf (1.0 mg/mL) for teas. All extract concentrations of herbal teas and cinnamon were able to enhance caspase-3 activity with lowest activity (4.4 mmol/ min/mL) observed in the lowest concentration of cinnamon (400 μg/mL) and highest activity (6.0 mmol/min/mL) seen in the highest concentration of raspberry leaf (2 mg/mL). Tea and spice extracts were able to induce apoptosis in Caco-2 cells exhibited by increased DNA fragmentation (expressed as enrichment factor). Enrichment factor ranged from 1.0 - 1.5 (raspberry leaf 1.0 mg/mL and hibiscus 10.0 mg/mL). Teas and cinnamon exhibited anti-inflammatory potential by inhibiting COX-2 by 0.6% - 8.0% (raspberry leaf 1.0 mg/mL and strawberry leaf 0.8 mg/mL). The results suggest that herbal teas and cinnamon may have significant benefits in chemoprevention.

Quantitative Estimation of γ-Glutamylethylamide in Commercially Available Made Teas [Camellia sinensis (L.) O. Kuntze, Theaceae] in Kenya

A study was carried out to quantitatively estimate the L-theanine content in 19 teas commercially available in the Kenyan market by High Performance Liquid Chromatography (HPLC). The test tea samples analyzed were green (n = 4), black (n = 8) and flavored (n = 7) teas from different origins viz., Kenya (n = 4), Uganda (n = 2), Tanzania (n = 5), Rwanda (n = 4), Cameroon (n = 1) and Sri-Lanka (n = 2) commercially available in the Kenyan market. The estimated Limit of Detection (LOD) of the current method was 0.01% L-theanine. The L-theanine content ranged from below the detection limit (<0.01% L-theanine) to 1.60% L-theanine on a dry weight (d.w) basis. Statistically significant differences (p < 0.05) were observed in the L-theanine contents of black, green and flavoured teas. Rwandan green tea contained the highest L-theanine content with 1.60% d.w. whereas six of the seven flavoured teas had very low theanine levels (<0.01%) that could not be quantified by the current method.

The Essential Oil Content and Composition of Selected Salvia Species, Sage Teas, and Sage Drops

Sage is popular for its manifold applications and has always been used in herbalism. The Salvia genus encompasses up to 900 species, but only a few of them have been investigated. Hence, the objective was to investigate the essential oil content as well as the terpene composition of a number of Saliva species. In this context, S. officinalis var. Extrakta, S. officinalis ＂Aurea＂ and S. patens were investigated for the first time. To some extent, it was also possible to associate the terpene distribution of the investigated commercial sage teas and drops with the processed sage oil. Furthermore, an ultrasonic extraction with ethyl acetate seems to be an efficient quick-method for extracting the essential oil.

Polyphenol Content and Antioxidant Effects in Herb Teas

Herb teas, Arabian jasmine, Balsam pear, Barley grass, Guava, Hardy rubber tree, Japanese persimmon, Jobs tears, Oolong tea, Puerh tea and Wolofberry have been consumed as beverages for health in Asia. Oxidative stress induces various diseases. Some of them, Arabian jasmine, Guava, Hardy rubber tree, Japanese per-simmon, Oolong tea and Puerh tea, have high total polyphenol content and antioxidant activities. Herbs and herbal polyphenols pay in controlling oxidation and prevent the damage by oxidation. These results showed that some of them, Arabian jasmine, Guava, Hardy rubber tree, Japanese persimmon, Oolong tea and Puerh tea, have high total polyphenol content and antioxidant activities. It has been demonstrated that high total polyphenol content in the herb teas provides high antioxidant activities.

Quality aspects of coffees and teas: Application of electron paramagnetic resonance (EPR) spectroscopy to the elucidation of free radical and other processes.

Coffees and teas are beverages that are both exceptionally rich in antioxidant molecules, and are also both associated with beneficial health effects. Thus although the quality characteristics of these beverages are conventionally assessed on the basis of their sensory properties, their antioxidant contents represent an additional and increasingly valued attribute of quality based on their contributions to healthy diets. Both beverages are prepared by hot water extraction of a pure plant-derived product, and thus their compositions can potentially change quite rapidly as a result of oxidation in contact with air. Oxidative processes often proceed via free radical intermediates, and sometimes also result in the formation of stable radical end-products;thus EPR spectroscopy is a convenient technique for investigating some of the various free radical reactions that occur in these beverages. This paper reviews progress that has been made in elucidating free radical processes that occur during the preparation and storage of coffees and teas, and the results are discussed in terms of quality criteria of the beverages.

经皮穴位电刺激联合中医情志干预对腹腔镜直肠癌根治术患者免疫功能和心理应激的影响

【目的】评价经皮穴位电刺激(TEAS)联合中医情志干预对腹腔镜直肠癌根治术患者免疫功能和心理应激的影响。【方法】将100例住院拟行腹腔镜直肠癌根治术治疗的患者随机分为A、B、C、D 4组,每组各25例。A组给予麻醉前TEAS 30 min,B组给予持续TEAS至手术结束,C组给予麻醉前TEAS 30 min+中医情志干预,D组给予持续TEAS至手术结束+中医情志干预,其中,TEAS取穴足三里(双侧)、三阴交(双侧)、百会、内关(双侧)。观察各组围术期指标以及术前与术后3 d免疫功能指标、Th1/Th2细胞分子机制、心理应激反应指标的变化情况,并比较各组的不良反应发生情况。【结果】(1)围术期指标方面,4组患者的手术时间、出血量、输液量比较,差异均无统计学意义(P>0.05);D组的舒芬太尼用量、瑞芬太尼用量、术后苏醒时间、术后24 h痛觉模拟量表(VAS)评分均低于A组、B组、C组(P<0.05),而B组、C组的上述指标均低于A组(P<0.05)。(2)免疫功能指标方面,D组术后3 d的T淋巴细胞亚群CD3+、CD4+及自然杀伤(NK)细胞水平均高于A组、B组、C组(P<0.05),而B组、C组的上述指标均高于A组(P<0.05)。(3)Th1/Th2细胞分子机制方面,D组术后3 d的血清白细胞介素2(IL-2)、γ干扰素(IFN-γ)水平均高于A组、B组、C组(P<0.05),血清白细胞介素10(IL-10)水平均低于A组、B组、C组(P<0.05),而B组、C组的血清IL-2、IFN-γ水平均高于A组(P<0.05),血清IL-10水平均低于A组(P<0.05)。(4)心理应激反应指标方面,D组术后3 d的焦虑自评量表(SAS)和抑郁自评量表(SDS)评分均低于A组、B组、C组(P<0.05),而B组、C组的上述评分均低于A组(P<0.05)。(5)不良反应方面,A组出现1例恶心呕吐,B组出现2例恶心呕吐、1例头晕,C组无不良反应,D组出现1例恶心呕吐,不良反应发生率分别为4.0%(1/25)、12.0%(3/25)、0.0%(0/25)、4.0%(1/25),组间比较,差异无统计学意义(χ2=0.400,P=0.527)。【结论】针对腹腔镜直肠癌根治术患者,TEAS联合中医情志干预可有效调节患者围术期免疫功能,减轻应激反应,缓解患者紧张焦虑状态,同时可产生一定的镇痛作用,减少阿片类药物用量,提高患者围术期舒适度及手术耐受水平。

任脉区段药物铺灸联合低频TEAS治疗少弱精子症临床观察

目的:观察任脉区段药物铺灸联合低频经皮穴位电刺激(TEAS)治疗少弱精子症的临床疗效。方法:将105例少弱精子症患者随机分为联合组、低频组和药物组,每组35例。药物组采用口服维生素E软胶囊、辅酶Q10胶囊、五子衍宗丸治疗;低频组取肾俞、脾俞进行TEAS治疗(2 Hz,10~15 mA),隔日1次,每次30 min;联合组在低频组的基础上,予以任脉区段(神阙至中极)药物铺灸治疗,每10天治疗1次。均治疗3个月。观察各组患者治疗前及治疗2、3个月后的精液常规(浓度、正常形态率、精子活力)、精浆超氧化物歧化酶(SOD)和丙二醛(MDA)含量及中医证候积分,并评定临床疗效。结果:(1)与治疗前比较,除药物组治疗2个月后精子形态指标外,3组患者治疗2、3个月后精液常规各项指标均改善(P<0.01);联合组治疗2、3个月后精液常规各项指标均优于药物组和低频组(P<0.01)。(2)与治疗前比较,3组患者治疗2、3个月后精浆SOD含量升高、MDA含量降低(P<0.01),且变化幅度联合组均大于其他两组(P<0.01)。(3)与治疗前比较,联合组治疗2、3个月后及药物组、低频组治疗3个月后中医证候积分降低(P<0.01);治疗2、3个月后,联合组中医证候积分低于药物组和低频组(P<0.01)。(4)联合组、药物组、低频组总有效率分别为85.7%(30/35)、68.6%(24/35)、65.7%(23/35),联合组临床疗效优于药物组和低频组(P<0.05)。结论:任脉区段药物铺灸联合低频TEAS治疗可改善少弱精子症患者的精液常规,降低精子氧化应激损伤,临床疗效优于药物和TEAS治疗。