Objective:This study is aimed to explore the effect of triazole fungicide tebuconazole(TEB)exposure on the oral absorption behavior of atenolol(AT),based on the homeostasis of bile acids(BAs).Methods:TEB was daily gav...Objective:This study is aimed to explore the effect of triazole fungicide tebuconazole(TEB)exposure on the oral absorption behavior of atenolol(AT),based on the homeostasis of bile acids(BAs).Methods:TEB was daily gavaged to rats with 3 mg/kg dose for 28 days to establish the TEB-exposure rat model.The amounts of glycocholic acid,glycochenodeoxycholic acid,taurocholic acid and taurine deoxycholic acid in the small intestine contents of normal and TEB-exposure rats were detected by LC-MS/MS.AT(10 mg/kg)were gavaged to the normal and TEB-exposure rats,and then blood were collected from orbital venous plexus at predetermined time-points.The concentration of AT in plasma was detected by LC-MS/MS,and the pharmacokinetic parameters were calculated by the DAS pharmacokinetic software.An intestinal circulation perfusion model was established in normal rats,and perfused with the perfusates containing the model drug of fluorescein and the BAs with the same compositions as the normal/TEB-exposure rats.After perfusion,the absorption and permeability of fluorescein in intestine were detected,as well as the oxidative stress status and ZO-1 expression level in the intestinal tissues.Results:Compared with normal rats,TEB-exposure increased the amounts of glycocholic acid,glycochenodeoxycholic acid,taurocholic acid and taurine deoxycholic acid in intestine significantly(P<0.001).In TEB-exposure rats,the maximum plasma concentration and area under the curve of AT were increased significantly than those of normal rats(P<0.05),and the peak time was significantly delayed(P<0.05).The TEB-induced BAs homeostasis perturbance increased intestinal permeability,and this effect was associated with the elevation of oxidative stress and the down-regulation of intercellular tight junction proteins in intestinal tissues.Conclusion:TEB-exposure can affect the oral absorption behavior of AT,which is probably related with the intestinal BAs homeostasis perturbance,thus it might affect the clinical efficacy and safety of this drug.展开更多
[Objective] This study was conducted to screen high-efficient low-toxicity new compound preparation for control of Glomerella cingulata. [Method] The toxicity of pyraclostrobin, tebuconazole and their mixed preparatio...[Objective] This study was conducted to screen high-efficient low-toxicity new compound preparation for control of Glomerella cingulata. [Method] The toxicity of pyraclostrobin, tebuconazole and their mixed preparations to Glomerella cingulata was determined by mycelial growth rate method, and their control effects on Glomerella cingulata were evaluated by field efficacy test. [Result] The mixed preparations of pyraclostrobin and tebuconazole at mass ratios of 5:1, 3:1, 1:1, 1:3 and 1:5 exhibited ECso values to mycelial growth of Glomerella cingulata of 0.612 9, 0.530 1, 0.232 6, 0.232 8 and 0.329 6 iJg/ml, respectively; and the 5 preparations exhibit- ed synergistic coefficients (SRs) to Glomerella cingulata of 1.29, 1.33, 2.29, 1.84 and 1.22, respectively, and among them, the preparation with mass ratio of 1:1 showed the highest synergism. The field efficacy test showed that the field control effects of 1 000 times, 2 000 times and 3 000 times dilutions of 25% pyraclostrobin-tebuconazole suspension, 5 000 times dilution of 430 g/L tebuconazole suspension and 2 000 times dilution of 250 g/L pyraclostrobin EC were 91.54%, 90.80%, 82.88%, 76.43% and 74.10%, respectively. [Conclusions] Therefore, the mixed preparations of pyraclostrobin and tebuconazole showed remarkable synergism on control of Glomerella cingulata, and preparation with the mixing ratio of 1:1 exhibited the most significant synergism on Glomerella cingulata. For soaking treatment to fruit clusters with 1 000-2 000 times dilution of 25% pyraclostrobin .tebuconazole suspension, the field efficacy to Glomerella cingulata was over 90%.展开更多
In order to test the control effect of 6% tebuconazole FS on maize head smut and to select its optimal dose for mixing seed, field tests were performed in 2012 and 2013. The results showed that 6% tebuconazole FS was ...In order to test the control effect of 6% tebuconazole FS on maize head smut and to select its optimal dose for mixing seed, field tests were performed in 2012 and 2013. The results showed that 6% tebuconazole FS was effective to control maize head smut, and the control effect ranged from 73.4% to 91.1%. In addition, it also improved the maize yield by 36.7%-44.1%, compared with the blank control. The optimal dose of 6% tebuconazole FS for seed dressing was 166.5 g/100 kg to control head smut on maize.展开更多
Fusarium head blight caused by Fusarium graminearum is one of the most serious diseases in wheat production worldwide, which seriously affects the yield and quality of wheat. Wheat grains carrying the pathogen are poi...Fusarium head blight caused by Fusarium graminearum is one of the most serious diseases in wheat production worldwide, which seriously affects the yield and quality of wheat. Wheat grains carrying the pathogen are poisonous to human and livestock health. With the long-term use of single chemical pesticide, the drug-resistance of F. graminearum is increasing day by day, and mixed agent is one of the effective strategies to improve the efficacy and delay the resistance. The mycelial growth rate method was used to determine the inhibition rates of phenamacril, tebuconazole and their mixtures(1∶1, 1∶3, 1∶5, 1∶7, 3∶1, 5∶1 and 7∶1). The synergistic coefficient(SR) was calculated by Wadley method, and the combined action types of the two agents were evaluated. The results showed that the ECof tebuconazole was 0.418 28 mg/L, and that of phenamacril was 0.489 8 mg/L; the optimum mixture ratio of phenamacril and tebuconazole was 1 ∶1, with the ECvalue of 0.053 74 and the synergistic coefficient of 4.2696, and the control effect of mixed agent was significantly better than that of single agent. It is feasible to control Fusarium head blight by mixing phenamacril and tebuconazole, providing a theoretical basis for development of new compound agents and effective control of Fusarium head blight.展开更多
Wheat scab caused by Fusarium graminearum is increasingly severe in the middle and lower reaches of the Yangtze River region. With long-term use of single chemical pesticide,the drug resistance of F. graminearum is gr...Wheat scab caused by Fusarium graminearum is increasingly severe in the middle and lower reaches of the Yangtze River region. With long-term use of single chemical pesticide,the drug resistance of F. graminearum is growing day by day. In order to reveal the inhibition effect and synergistic effect of prochloraz and tebuconazole and their mixture on F. graminearum,new compound agents for controlling wheat scab were developed. The inhibition effect and synergistic effect of prochloraz and tebuconazole mixed at seven different ratios on F. graminearum were studied. The results showed that the efficiency of the mixture was the best when the proportion of prochloraz and tebuconazole was 1:7; the EC50 was 0.145 36 and the synergistic coefficient was 1.853 9. The results provide a theoretical basis for development of new compound agents and effective control of wheat scab.展开更多
[ Objective] The study aimed to determine tebuconazole residue in apples and vegetables using matrix solid phase dispersion-gas chro- matography (MSPD-GC). [ Method] The effects of extraction and determination condi...[ Objective] The study aimed to determine tebuconazole residue in apples and vegetables using matrix solid phase dispersion-gas chro- matography (MSPD-GC). [ Method] The effects of extraction and determination conditions on the detection of tebuconazole left in apples and veg- etables were analyzed, and the optimum extraction conditions were determined. [ Result] The recovery rate of tebucenazole was the highest when the ratio of a sample to florisil dispersant was 1 : 4, and the mixture of hexane and acetone ( 1 : 1 ) with total volume of 8 ml was as the eluant. Under the optimum conditions, the relative standard deviation (RSD) of the method was 4.9% -7.6%, and the detection limit was 0.1 tJg/g, while the re- covery rate of tebuconazole changed from 86.7% to 95. 2% . [ Conclusion] The method was simple, accurate, sensitive and applicable to the de- termination of tebuconazole in aaricultural Droducts.展开更多
[ Objective] The paper was to confirm the best application period and frequency of 75% trifloxystrobin ·tebuconazole WG against rice blast and rice sheath blight. [ Method] Influences of different dosages and dif...[ Objective] The paper was to confirm the best application period and frequency of 75% trifloxystrobin ·tebuconazole WG against rice blast and rice sheath blight. [ Method] Influences of different dosages and different application periods of 75% trifloxystrobin · tebuconazole WG on control effects against rice blast and rice sheath blight, as well as their effects on rice yields were studied in the paper. [Result] The control effects of three different fungicides application treatments against rice sheath blight were 80.24%, 83.0% and 67.99%, and the control effects against rice blast were 56.4%, 49.11% and 61.1%, respective- ly. Advanced application of fungicide for two times had good prevention effect against rice sheath blight ; properly delayed application of fungicides for two times was conducive to improving the control effect against rice blast, and one time application of sufficient fungicide during middle booting stage had higher control effect than application for two times. Effective panicle number per unit area, total grain number, 1 000-grain weight and moisture content of various fungicide application treat- ments did not have significant difference with those of the treatments without fungicide application, but grain number per panicle in treatments applied with fungicide was higher than the treatment without application. [ Conclusion] 75% Trifloxystrobin ·tebuconazole WG has better control effect on rice blast and sheath blight, which helps to promote the formation of rice grain and increases yield significantly. The fungicide application against rice sheath blight should be appropriately ad- vanced, and application for one time against panicle blast after middle booting stage is helpful to improve the control effect.展开更多
目的探究综合运用白蛋白和光疗对治疗重度新生儿高胆红素血症的效果及对患儿总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TSB)水平的影响。方法选择2020年1月—2022年12月于淮安市淮阴区妇幼保健院接受临床干预的85例重...目的探究综合运用白蛋白和光疗对治疗重度新生儿高胆红素血症的效果及对患儿总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TSB)水平的影响。方法选择2020年1月—2022年12月于淮安市淮阴区妇幼保健院接受临床干预的85例重度新生儿高胆红素血症的患儿为研究对象,按照其接受干预方式分为研究组(45例)和对照组(40例)。研究组联用白蛋白与光疗治疗,对照组接受单纯光疗治疗。比较两组患儿临床干预效果、干预过程中TBA、TSB等实验室指标的变化情况,并统计两组患儿治疗过程中并发症的发生情况。结果研究组患者治疗总有效率高于对照组,差异有统计学意义(P<0.05)。干预12~72 h,研究组患儿的TBA和TSB水平均低于对照组,差异有统计学意义(P<0.05)。干预前,两组患儿超敏C反应蛋白(HS-CRP)、游离脂肪酸(FFA)水平比较,差异无统计学意义(P>0.05);干预后,研究组患儿HS-CRP、FFA水平均低于对照组,差异有统计学意义(P<0.05)。两组患儿不良反应发生率比较,差异无统计学意义(P>0.05)。结论白蛋白联合光疗对重度新生儿高胆红素血症患儿具有较好的干预效果,能够显著降低新生儿TBA及TSB水平,治疗安全性较好,值得临床应用。展开更多
Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with...Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with hepatitis B-related decompensated cirrhosis,and study group 2 consisted of 30 patients with hepatitis B;while the control group consisted of 30 healthy people who underwent physical examination.The blood parameters were used to evaluate the clinical treatment effect of patients.Results:The TBA,Cys-C,alanine transaminase(ALT),total bilirubin(TBIL),aspartate aminotransferase(AST),and international normalized ratio(INR)in study group 1 were significantly higher than those of study group 2 and the control group;while the platelet count(PLT),hemoglobin(Hb),albumin(ALB),and estimated glomerular filtration rate(eGFR)were significantly lower in the study group 1 compared to the control group and study group 2(P<0.05).The Cys-C,PLT,TBA,AST,TBIL,and INR of patients in study group 1 who were successfully treated were significantly lower than the patients who were not successfully treated(P<0.05).Conclusion:Serum Cys-C,TBA,and routine blood parameters are useful in predicting the condition and the prognosis of patients of hepatitis B-related decompensated cirrhosis.展开更多
基金supported by National Natural Science Foundation of China(81874348)Academic Support Program for Top Talents of Higher Education in Anhui Province(GXBJZD2022027)Science and Technology Innovation Project of Anhui Drug Administration(AHYJ-KJ-202110)。
文摘Objective:This study is aimed to explore the effect of triazole fungicide tebuconazole(TEB)exposure on the oral absorption behavior of atenolol(AT),based on the homeostasis of bile acids(BAs).Methods:TEB was daily gavaged to rats with 3 mg/kg dose for 28 days to establish the TEB-exposure rat model.The amounts of glycocholic acid,glycochenodeoxycholic acid,taurocholic acid and taurine deoxycholic acid in the small intestine contents of normal and TEB-exposure rats were detected by LC-MS/MS.AT(10 mg/kg)were gavaged to the normal and TEB-exposure rats,and then blood were collected from orbital venous plexus at predetermined time-points.The concentration of AT in plasma was detected by LC-MS/MS,and the pharmacokinetic parameters were calculated by the DAS pharmacokinetic software.An intestinal circulation perfusion model was established in normal rats,and perfused with the perfusates containing the model drug of fluorescein and the BAs with the same compositions as the normal/TEB-exposure rats.After perfusion,the absorption and permeability of fluorescein in intestine were detected,as well as the oxidative stress status and ZO-1 expression level in the intestinal tissues.Results:Compared with normal rats,TEB-exposure increased the amounts of glycocholic acid,glycochenodeoxycholic acid,taurocholic acid and taurine deoxycholic acid in intestine significantly(P<0.001).In TEB-exposure rats,the maximum plasma concentration and area under the curve of AT were increased significantly than those of normal rats(P<0.05),and the peak time was significantly delayed(P<0.05).The TEB-induced BAs homeostasis perturbance increased intestinal permeability,and this effect was associated with the elevation of oxidative stress and the down-regulation of intercellular tight junction proteins in intestinal tissues.Conclusion:TEB-exposure can affect the oral absorption behavior of AT,which is probably related with the intestinal BAs homeostasis perturbance,thus it might affect the clinical efficacy and safety of this drug.
基金Supported by Jiangsu Agricultural Science and Technology Independent Innovation Fund(CX(14)2056)Science & Technology Specific Project for Enriching People and Strengthening County Economy in China(BN20156222)~~
文摘[Objective] This study was conducted to screen high-efficient low-toxicity new compound preparation for control of Glomerella cingulata. [Method] The toxicity of pyraclostrobin, tebuconazole and their mixed preparations to Glomerella cingulata was determined by mycelial growth rate method, and their control effects on Glomerella cingulata were evaluated by field efficacy test. [Result] The mixed preparations of pyraclostrobin and tebuconazole at mass ratios of 5:1, 3:1, 1:1, 1:3 and 1:5 exhibited ECso values to mycelial growth of Glomerella cingulata of 0.612 9, 0.530 1, 0.232 6, 0.232 8 and 0.329 6 iJg/ml, respectively; and the 5 preparations exhibit- ed synergistic coefficients (SRs) to Glomerella cingulata of 1.29, 1.33, 2.29, 1.84 and 1.22, respectively, and among them, the preparation with mass ratio of 1:1 showed the highest synergism. The field efficacy test showed that the field control effects of 1 000 times, 2 000 times and 3 000 times dilutions of 25% pyraclostrobin-tebuconazole suspension, 5 000 times dilution of 430 g/L tebuconazole suspension and 2 000 times dilution of 250 g/L pyraclostrobin EC were 91.54%, 90.80%, 82.88%, 76.43% and 74.10%, respectively. [Conclusions] Therefore, the mixed preparations of pyraclostrobin and tebuconazole showed remarkable synergism on control of Glomerella cingulata, and preparation with the mixing ratio of 1:1 exhibited the most significant synergism on Glomerella cingulata. For soaking treatment to fruit clusters with 1 000-2 000 times dilution of 25% pyraclostrobin .tebuconazole suspension, the field efficacy to Glomerella cingulata was over 90%.
文摘In order to test the control effect of 6% tebuconazole FS on maize head smut and to select its optimal dose for mixing seed, field tests were performed in 2012 and 2013. The results showed that 6% tebuconazole FS was effective to control maize head smut, and the control effect ranged from 73.4% to 91.1%. In addition, it also improved the maize yield by 36.7%-44.1%, compared with the blank control. The optimal dose of 6% tebuconazole FS for seed dressing was 166.5 g/100 kg to control head smut on maize.
基金Supported by Open Project Program of Engineering Research Center of Ecology and Agricultural Use of Wetland,the Ministry of Education(KF201802)
文摘Fusarium head blight caused by Fusarium graminearum is one of the most serious diseases in wheat production worldwide, which seriously affects the yield and quality of wheat. Wheat grains carrying the pathogen are poisonous to human and livestock health. With the long-term use of single chemical pesticide, the drug-resistance of F. graminearum is increasing day by day, and mixed agent is one of the effective strategies to improve the efficacy and delay the resistance. The mycelial growth rate method was used to determine the inhibition rates of phenamacril, tebuconazole and their mixtures(1∶1, 1∶3, 1∶5, 1∶7, 3∶1, 5∶1 and 7∶1). The synergistic coefficient(SR) was calculated by Wadley method, and the combined action types of the two agents were evaluated. The results showed that the ECof tebuconazole was 0.418 28 mg/L, and that of phenamacril was 0.489 8 mg/L; the optimum mixture ratio of phenamacril and tebuconazole was 1 ∶1, with the ECvalue of 0.053 74 and the synergistic coefficient of 4.2696, and the control effect of mixed agent was significantly better than that of single agent. It is feasible to control Fusarium head blight by mixing phenamacril and tebuconazole, providing a theoretical basis for development of new compound agents and effective control of Fusarium head blight.
基金Supported by Natural Science Foundation of Hubei Province(2016CFB478)Youth Talent Program of Hubei Department of Education(D200712004)
文摘Wheat scab caused by Fusarium graminearum is increasingly severe in the middle and lower reaches of the Yangtze River region. With long-term use of single chemical pesticide,the drug resistance of F. graminearum is growing day by day. In order to reveal the inhibition effect and synergistic effect of prochloraz and tebuconazole and their mixture on F. graminearum,new compound agents for controlling wheat scab were developed. The inhibition effect and synergistic effect of prochloraz and tebuconazole mixed at seven different ratios on F. graminearum were studied. The results showed that the efficiency of the mixture was the best when the proportion of prochloraz and tebuconazole was 1:7; the EC50 was 0.145 36 and the synergistic coefficient was 1.853 9. The results provide a theoretical basis for development of new compound agents and effective control of wheat scab.
基金Supported by the Practice Innovation Training Program of Undergraduates in Jiangsu Province,China
文摘[ Objective] The study aimed to determine tebuconazole residue in apples and vegetables using matrix solid phase dispersion-gas chro- matography (MSPD-GC). [ Method] The effects of extraction and determination conditions on the detection of tebuconazole left in apples and veg- etables were analyzed, and the optimum extraction conditions were determined. [ Result] The recovery rate of tebucenazole was the highest when the ratio of a sample to florisil dispersant was 1 : 4, and the mixture of hexane and acetone ( 1 : 1 ) with total volume of 8 ml was as the eluant. Under the optimum conditions, the relative standard deviation (RSD) of the method was 4.9% -7.6%, and the detection limit was 0.1 tJg/g, while the re- covery rate of tebuconazole changed from 86.7% to 95. 2% . [ Conclusion] The method was simple, accurate, sensitive and applicable to the de- termination of tebuconazole in aaricultural Droducts.
基金Supported by National Science and Technology Projects (2012BAD19B03).
文摘[ Objective] The paper was to confirm the best application period and frequency of 75% trifloxystrobin ·tebuconazole WG against rice blast and rice sheath blight. [ Method] Influences of different dosages and different application periods of 75% trifloxystrobin · tebuconazole WG on control effects against rice blast and rice sheath blight, as well as their effects on rice yields were studied in the paper. [Result] The control effects of three different fungicides application treatments against rice sheath blight were 80.24%, 83.0% and 67.99%, and the control effects against rice blast were 56.4%, 49.11% and 61.1%, respective- ly. Advanced application of fungicide for two times had good prevention effect against rice sheath blight ; properly delayed application of fungicides for two times was conducive to improving the control effect against rice blast, and one time application of sufficient fungicide during middle booting stage had higher control effect than application for two times. Effective panicle number per unit area, total grain number, 1 000-grain weight and moisture content of various fungicide application treat- ments did not have significant difference with those of the treatments without fungicide application, but grain number per panicle in treatments applied with fungicide was higher than the treatment without application. [ Conclusion] 75% Trifloxystrobin ·tebuconazole WG has better control effect on rice blast and sheath blight, which helps to promote the formation of rice grain and increases yield significantly. The fungicide application against rice sheath blight should be appropriately ad- vanced, and application for one time against panicle blast after middle booting stage is helpful to improve the control effect.
文摘目的探究综合运用白蛋白和光疗对治疗重度新生儿高胆红素血症的效果及对患儿总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TSB)水平的影响。方法选择2020年1月—2022年12月于淮安市淮阴区妇幼保健院接受临床干预的85例重度新生儿高胆红素血症的患儿为研究对象,按照其接受干预方式分为研究组(45例)和对照组(40例)。研究组联用白蛋白与光疗治疗,对照组接受单纯光疗治疗。比较两组患儿临床干预效果、干预过程中TBA、TSB等实验室指标的变化情况,并统计两组患儿治疗过程中并发症的发生情况。结果研究组患者治疗总有效率高于对照组,差异有统计学意义(P<0.05)。干预12~72 h,研究组患儿的TBA和TSB水平均低于对照组,差异有统计学意义(P<0.05)。干预前,两组患儿超敏C反应蛋白(HS-CRP)、游离脂肪酸(FFA)水平比较,差异无统计学意义(P>0.05);干预后,研究组患儿HS-CRP、FFA水平均低于对照组,差异有统计学意义(P<0.05)。两组患儿不良反应发生率比较,差异无统计学意义(P>0.05)。结论白蛋白联合光疗对重度新生儿高胆红素血症患儿具有较好的干预效果,能够显著降低新生儿TBA及TSB水平,治疗安全性较好,值得临床应用。
基金SPPH Incubator Fund for Development of Science and Technology(2021YJY-19)SPPH Foundation for Development of Science and Technology(2021BJ-26)International Science and Technology Cooperation Projects of Shaanxi Province(2022KW-14).
文摘Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with hepatitis B-related decompensated cirrhosis,and study group 2 consisted of 30 patients with hepatitis B;while the control group consisted of 30 healthy people who underwent physical examination.The blood parameters were used to evaluate the clinical treatment effect of patients.Results:The TBA,Cys-C,alanine transaminase(ALT),total bilirubin(TBIL),aspartate aminotransferase(AST),and international normalized ratio(INR)in study group 1 were significantly higher than those of study group 2 and the control group;while the platelet count(PLT),hemoglobin(Hb),albumin(ALB),and estimated glomerular filtration rate(eGFR)were significantly lower in the study group 1 compared to the control group and study group 2(P<0.05).The Cys-C,PLT,TBA,AST,TBIL,and INR of patients in study group 1 who were successfully treated were significantly lower than the patients who were not successfully treated(P<0.05).Conclusion:Serum Cys-C,TBA,and routine blood parameters are useful in predicting the condition and the prognosis of patients of hepatitis B-related decompensated cirrhosis.