During the boll formation stage,cotton bolls exhibit the lowest expression of Bacillus thuringiensis(Bt)insecticidal proteins.Resistance to insects varies notably among different organs,which poses challenges for cont...During the boll formation stage,cotton bolls exhibit the lowest expression of Bacillus thuringiensis(Bt)insecticidal proteins.Resistance to insects varies notably among different organs,which poses challenges for controlling cotton bollworms.Consequently,an experimental strategy was designed in the 2020-2021 cotton growing season to coordinate the enhancement of protein synthesis and the attenuation of degradation.Two Bt cultivars of Gossypium hirsutum,namely the hybrid Sikang 3 and the conventional Sikang 1,were used as test materials.Three treatments were applied at the peak flowering period:CK(the control),T1(amino acids),and T2(amino acids and EDTA).The results show that,in comparison to the CK group,the Bt protein contents were significantly increased in both cotton bolls and their subtending leaves under the T1 and T2 treatments.The maximum levels of increase observed were 67.5%in cotton bolls and 21.7%in leaves.Moreover,the disparity in Bt protein content between cotton bolls and their subtending leaves notably decreased by 31.2%.Correlation analysis suggested that the primary physiological mechanisms for augmenting Bt protein content involve increased protein synthesis and reduced protein catabolism,which are independent of Bt gene expression levels.Stepwise regression and path analysis revealed that elevating the soluble protein content and transaminase activity,while reducing the catabolic enzyme activities,are instrumental in enhancing the Bt protein content.Consequently,the coordinated application of amino acids and EDTA emerges as a strategy that can improve the overall resistance of Bt cotton and mitigate the spatiotemporal variations in Bt toxin concentrations in both cotton bolls and leaves.展开更多
An earthquake of Ms=7.4 occurred in Mani, Xizang (Tibet), China on November 8, 1997. The moment tensor ofthis earthquake was inverted using the long period body wave form data from China Digital Seismograph Network(CD...An earthquake of Ms=7.4 occurred in Mani, Xizang (Tibet), China on November 8, 1997. The moment tensor ofthis earthquake was inverted using the long period body wave form data from China Digital Seismograph Network(CDSN). The apparent source time functions (AS TFs) were retrieved from P and S waves, respectively, using thedeconvolution technique in frequency domain, and the tempo-spatial rupture process on the fault plane was imagedby inverting the azimuth dependent AS TFs from different stations. The result of the moment tensor inversionindicates that the P and T axes of earthquake-generating stress field were nearly horizontal, with the P axis in theNNE direction (29), the T axis in the SEE direction (122) and that the NEE-SWW striking nodal plane andNNW-SSE striking nodal plane are mainly left-lateral and right-lateral strike-slip, respectively; that this earthquakehad a scalar seismic moment of 3.4xl02o N. .m, and a moment magnitude of Mw=7.6. Taking the aftershock distribution into account, we proposed that the earthquake rupture occurred in the fault plane with the strike of 250,the dip of 88 and the rake of 19. On the basis of the result of the moment tensor inversion, the theoretical seismograms were synthesized, and then the AS T Fs were retrieved by deconvoving the synthetic seismograms fromthe observed seismograms. The A S T Fs retrieved from the P and S waves of different stations identically suggestedthat this earthquake was of a simple time history, whose ASTF can be approximated with a sine function with thehalf period of about 10 s. Inverting the azimuth dependent A S T Fs from P and S waveforms led to the imageshowing the tempo-spatial distribution of the rupture on the fault plane. From the 'remembering' snap-shots, therupture initiated at the western end of the fault, and then propagated eastward and downward, indicating an overallunilateral rupture. However, the slip distribution is non-uniform, being made up of three sub-areas, one in thewestern end, about 10 km deep ('western area'), another about 55 kin away from the western end and about 35 Iondeep ('eastern area'), the third about 30 km away from the western end and around 40 km deep ('central area').The total rupture area was around 70 km long and 60 km wide. From the 'forgetting' snap-shots, the rupturingappeared quite complex, with the slip occurring in different position at different time, and the earthquake being ofthe characteristics of 'healing pulse'. Another point we have to stress is that the locations in which the ruptureinitiated and terminated were not where the main rupture took place. Eventually, the static slip distribution wascalculated, and the largest slip values of the three sub-areas were 956 cm, 743 cm and 1 060 cm, for the western.eastern and central areas, respectively. From the slip distribution, the rupture mainly distributed in the fault about70 km eastern to the epicenter; from the aftershock distribution. however, the aftershocks were very sparse in thewest to the epicenter while densely clustered in the east to the epicenter It indicated that the Maul Ms=7.9 earthquake was resulted from the nearly eastward extension of the NEE-SWW to nearly E-W striking fault in thenorthwestern Tibetan plateau.展开更多
In Bacillus thuringenesis(Bt) transgenic cotton, the cotton boll has the lowest insecticidal protein content when compared to the other organs. The present study investigated the effects of amino acid spray applicatio...In Bacillus thuringenesis(Bt) transgenic cotton, the cotton boll has the lowest insecticidal protein content when compared to the other organs. The present study investigated the effects of amino acid spray application at the peak flowering stage on the cotton boll Bt toxin concentration and yield formation. Boll protein synthesis and carbohydrate conversion were also studied to reveal the fundamental mechanism. Three treatments(i.e., CK, the untreated control;LA1, five amino acids;LA2, 21 amino acids) were applied to two Bt cultivars of G. hirsutum(i.e., the hybrid Sikang 3 and the conventional Sikang 1) in the cotton-growing seasons during 2017 and 2018. Amino acid spray application at the peak flowering stage resulted in an increase of 5.2–16.4% in the boll Bt protein concentration and an increase of 5.5–11.3%in the seed cotton yield, but there was no difference between the two amino acid treatments. In addition, amino acid applications led to increases in the amino acid content, soluble protein content, glutamate pyruvate transaminase(GPT)activity, glutamate oxaloacetate transaminase(GOT) activity, glucose content, fructose content and soluble acid invertase(SAI) activity. This study also found that Bt protein content, enhanced boll number and the weight of opened bolls were closely related to carbon and nitrogen metabolism. The Bt protein content had significant linear positive correlations with amino acid and soluble protein contents. Enhanced boll number had significant linear positive correlations with the GPT and GOT activities from 15–25 days after flowering(DAF). The weight of opened bolls from 55–65 DAF had a significant linear positive correlation with the SAI activity. These results indicate that the enhancement of boll protein synthesis and carbohydrate conversion by amino acid application resulted in a simultaneous increase in the boll Bt protein concentration and cotton lint yield.展开更多
[Objective] This study aimed to explore an optimum application amount of nitrogen for cotton cultivation. [Method] In this study, a field experiment was conducted to investigate the effects of nitrogen application amo...[Objective] This study aimed to explore an optimum application amount of nitrogen for cotton cultivation. [Method] In this study, a field experiment was conducted to investigate the effects of nitrogen application amount on the growth characteristics, boll development and lint yield of high quality cotton line FZ-1. [Result] Compared with the nitrogen level of 225 kg/hm2, the lint yield had increased by 28.46% and 18.73%, respectively, with the nitrogen application amount of 300 and 375 kg/hm2. When the nitrogen application amount had increased from 225 to 300 kg/hm2, boll number per plant, boll weight and lint yield had significantly increased. At the nitrogen level of 375 kg/hm2, however, the effects of increasing lint yield were significantly less than that at the nitrogen level of 300 kg/hm2. Compared with the nitrogen levels of 225 and 375, 300 kg/hm2 of nitrogen was the optimum application amount to improve the plant height, daily increment of plant height, number of fruit branches, number ratio of nodes to fruit branches, boll volume and seed cotton weight per boll. [Conclusion] The rational management of nitrogen is the most effective way to promote the growth and development of cotton plants, ensure high yielding ability and minimize the environmental pollution caused by the overuse of nitrogen. This study had provided a sound nitrogen application strategy for the cultivation of this high-quality cotton line in the field plantation.展开更多
基金supported by the National Natural Science Foundation of China(31901462 and 31671613)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(22KJA210005)+1 种基金the China Scholarship Council(202308320440)the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX22_3508)。
文摘During the boll formation stage,cotton bolls exhibit the lowest expression of Bacillus thuringiensis(Bt)insecticidal proteins.Resistance to insects varies notably among different organs,which poses challenges for controlling cotton bollworms.Consequently,an experimental strategy was designed in the 2020-2021 cotton growing season to coordinate the enhancement of protein synthesis and the attenuation of degradation.Two Bt cultivars of Gossypium hirsutum,namely the hybrid Sikang 3 and the conventional Sikang 1,were used as test materials.Three treatments were applied at the peak flowering period:CK(the control),T1(amino acids),and T2(amino acids and EDTA).The results show that,in comparison to the CK group,the Bt protein contents were significantly increased in both cotton bolls and their subtending leaves under the T1 and T2 treatments.The maximum levels of increase observed were 67.5%in cotton bolls and 21.7%in leaves.Moreover,the disparity in Bt protein content between cotton bolls and their subtending leaves notably decreased by 31.2%.Correlation analysis suggested that the primary physiological mechanisms for augmenting Bt protein content involve increased protein synthesis and reduced protein catabolism,which are independent of Bt gene expression levels.Stepwise regression and path analysis revealed that elevating the soluble protein content and transaminase activity,while reducing the catabolic enzyme activities,are instrumental in enhancing the Bt protein content.Consequently,the coordinated application of amino acids and EDTA emerges as a strategy that can improve the overall resistance of Bt cotton and mitigate the spatiotemporal variations in Bt toxin concentrations in both cotton bolls and leaves.
文摘An earthquake of Ms=7.4 occurred in Mani, Xizang (Tibet), China on November 8, 1997. The moment tensor ofthis earthquake was inverted using the long period body wave form data from China Digital Seismograph Network(CDSN). The apparent source time functions (AS TFs) were retrieved from P and S waves, respectively, using thedeconvolution technique in frequency domain, and the tempo-spatial rupture process on the fault plane was imagedby inverting the azimuth dependent AS TFs from different stations. The result of the moment tensor inversionindicates that the P and T axes of earthquake-generating stress field were nearly horizontal, with the P axis in theNNE direction (29), the T axis in the SEE direction (122) and that the NEE-SWW striking nodal plane andNNW-SSE striking nodal plane are mainly left-lateral and right-lateral strike-slip, respectively; that this earthquakehad a scalar seismic moment of 3.4xl02o N. .m, and a moment magnitude of Mw=7.6. Taking the aftershock distribution into account, we proposed that the earthquake rupture occurred in the fault plane with the strike of 250,the dip of 88 and the rake of 19. On the basis of the result of the moment tensor inversion, the theoretical seismograms were synthesized, and then the AS T Fs were retrieved by deconvoving the synthetic seismograms fromthe observed seismograms. The A S T Fs retrieved from the P and S waves of different stations identically suggestedthat this earthquake was of a simple time history, whose ASTF can be approximated with a sine function with thehalf period of about 10 s. Inverting the azimuth dependent A S T Fs from P and S waveforms led to the imageshowing the tempo-spatial distribution of the rupture on the fault plane. From the 'remembering' snap-shots, therupture initiated at the western end of the fault, and then propagated eastward and downward, indicating an overallunilateral rupture. However, the slip distribution is non-uniform, being made up of three sub-areas, one in thewestern end, about 10 km deep ('western area'), another about 55 kin away from the western end and about 35 Iondeep ('eastern area'), the third about 30 km away from the western end and around 40 km deep ('central area').The total rupture area was around 70 km long and 60 km wide. From the 'forgetting' snap-shots, the rupturingappeared quite complex, with the slip occurring in different position at different time, and the earthquake being ofthe characteristics of 'healing pulse'. Another point we have to stress is that the locations in which the ruptureinitiated and terminated were not where the main rupture took place. Eventually, the static slip distribution wascalculated, and the largest slip values of the three sub-areas were 956 cm, 743 cm and 1 060 cm, for the western.eastern and central areas, respectively. From the slip distribution, the rupture mainly distributed in the fault about70 km eastern to the epicenter; from the aftershock distribution. however, the aftershocks were very sparse in thewest to the epicenter while densely clustered in the east to the epicenter It indicated that the Maul Ms=7.9 earthquake was resulted from the nearly eastward extension of the NEE-SWW to nearly E-W striking fault in thenorthwestern Tibetan plateau.
基金supported by the National Natural Science Foundation of China (31901462 and 31671613)the Natural Science Foundation of Jiangsu Province,China (BK20191439)+1 种基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX22_3508)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China (PAPD)。
文摘In Bacillus thuringenesis(Bt) transgenic cotton, the cotton boll has the lowest insecticidal protein content when compared to the other organs. The present study investigated the effects of amino acid spray application at the peak flowering stage on the cotton boll Bt toxin concentration and yield formation. Boll protein synthesis and carbohydrate conversion were also studied to reveal the fundamental mechanism. Three treatments(i.e., CK, the untreated control;LA1, five amino acids;LA2, 21 amino acids) were applied to two Bt cultivars of G. hirsutum(i.e., the hybrid Sikang 3 and the conventional Sikang 1) in the cotton-growing seasons during 2017 and 2018. Amino acid spray application at the peak flowering stage resulted in an increase of 5.2–16.4% in the boll Bt protein concentration and an increase of 5.5–11.3%in the seed cotton yield, but there was no difference between the two amino acid treatments. In addition, amino acid applications led to increases in the amino acid content, soluble protein content, glutamate pyruvate transaminase(GPT)activity, glutamate oxaloacetate transaminase(GOT) activity, glucose content, fructose content and soluble acid invertase(SAI) activity. This study also found that Bt protein content, enhanced boll number and the weight of opened bolls were closely related to carbon and nitrogen metabolism. The Bt protein content had significant linear positive correlations with amino acid and soluble protein contents. Enhanced boll number had significant linear positive correlations with the GPT and GOT activities from 15–25 days after flowering(DAF). The weight of opened bolls from 55–65 DAF had a significant linear positive correlation with the SAI activity. These results indicate that the enhancement of boll protein synthesis and carbohydrate conversion by amino acid application resulted in a simultaneous increase in the boll Bt protein concentration and cotton lint yield.
基金Supported by Natural Science Foundation of China(NSFC No.30771272,31171483)the Priority Academic Development Program of Jiangsu Higher Education Institutions+1 种基金Jiangsu Innovation Project for Agriculture Science and Technology(cx(11)2054)Jiangsu Agriculture Science and Technology Support Program(SBE2010307)~~
文摘[Objective] This study aimed to explore an optimum application amount of nitrogen for cotton cultivation. [Method] In this study, a field experiment was conducted to investigate the effects of nitrogen application amount on the growth characteristics, boll development and lint yield of high quality cotton line FZ-1. [Result] Compared with the nitrogen level of 225 kg/hm2, the lint yield had increased by 28.46% and 18.73%, respectively, with the nitrogen application amount of 300 and 375 kg/hm2. When the nitrogen application amount had increased from 225 to 300 kg/hm2, boll number per plant, boll weight and lint yield had significantly increased. At the nitrogen level of 375 kg/hm2, however, the effects of increasing lint yield were significantly less than that at the nitrogen level of 300 kg/hm2. Compared with the nitrogen levels of 225 and 375, 300 kg/hm2 of nitrogen was the optimum application amount to improve the plant height, daily increment of plant height, number of fruit branches, number ratio of nodes to fruit branches, boll volume and seed cotton weight per boll. [Conclusion] The rational management of nitrogen is the most effective way to promote the growth and development of cotton plants, ensure high yielding ability and minimize the environmental pollution caused by the overuse of nitrogen. This study had provided a sound nitrogen application strategy for the cultivation of this high-quality cotton line in the field plantation.