[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, ...[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.展开更多
Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing p...Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R<sub>0</sub> - 1);2) when the plants shed (R<sub>0</sub>) and 3) at two weeks after the plants shed (R<sub>0</sub> + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms.展开更多
In the 1970s and 1980s, United States Department of Energy conducted numerous studies on the fabrication of nuclear fuel particles using the internal gelation process[1], the internal gelation process is an advanced p...In the 1970s and 1980s, United States Department of Energy conducted numerous studies on the fabrication of nuclear fuel particles using the internal gelation process[1], the internal gelation process is an advanced production process for nuclear fuels. Recently, we had used these lessons learned on the internal gelation process to prepare quantities of uranium dioxide kernels of uniform sizes. This paper discusses the whole efforts of experimental processes.展开更多
Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to p...Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to prepare protein samples of taro that was compatible with subsequent analysis using 2D-PAGE. We compared proteins from shoot basal region from 0 d and 2 d after the beginning of tuberization. By this method we got about (2 000) spots and high reproducibility. Additionally some changes of protein expression were found.展开更多
[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sod...[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.展开更多
Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stre...Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stress, the biomass allocations to different plant components and some related morphological parameters were examined along an elevational gradient within a salt marsh. Authors found that S. mariqueter performed best at medium elevation within the marsh, with relatively high density of shoot and individual ramet dry mass. Biomass allocation to corm was the highest at low elevations, and the least at high elevations, suggesting that a conservative strategy was adopted by the species to cope with the harsh physical conditions at the low elevation. The investment in rhizome decreased from low to high elevations, while the proportion of inflorescence mass increased, indicating that during the life history, the species shifts from predominant asexual reproduction to predominant sexual reproduction. This may be favourable for the species to colonize larger area, and to spread and persist at a meta_population level. Correlation analyses showed that sexual reproduction was inversely related to growth and asexual reproduction. However, it is difficult to determine the relationship between asexual reproduction and growth possibly because of the varied function of the corms of the species in different life history stages.展开更多
基金Supported by Special Fund for the Dominant Subjects Development of the Higher Education Institutions of Jiangsu ProvinceScience and Technology Innovation Fund of Nanjing Forestry University(X09-120-4)~~
文摘[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.
文摘Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R<sub>0</sub> - 1);2) when the plants shed (R<sub>0</sub>) and 3) at two weeks after the plants shed (R<sub>0</sub> + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms.
文摘In the 1970s and 1980s, United States Department of Energy conducted numerous studies on the fabrication of nuclear fuel particles using the internal gelation process[1], the internal gelation process is an advanced production process for nuclear fuels. Recently, we had used these lessons learned on the internal gelation process to prepare quantities of uranium dioxide kernels of uniform sizes. This paper discusses the whole efforts of experimental processes.
基金Item supported by science and technologycommittee of Shanghai municipality(003113010)
文摘Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to prepare protein samples of taro that was compatible with subsequent analysis using 2D-PAGE. We compared proteins from shoot basal region from 0 d and 2 d after the beginning of tuberization. By this method we got about (2 000) spots and high reproducibility. Additionally some changes of protein expression were found.
基金Supported by the National Natural Science Foundation(30670177)Scientific Research Foundation for the Outstanding Young Scientist of Shangdong Province(006BS06002)National Ministry of Education Doctoral Fund(20050445003)~~
文摘[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.
文摘Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stress, the biomass allocations to different plant components and some related morphological parameters were examined along an elevational gradient within a salt marsh. Authors found that S. mariqueter performed best at medium elevation within the marsh, with relatively high density of shoot and individual ramet dry mass. Biomass allocation to corm was the highest at low elevations, and the least at high elevations, suggesting that a conservative strategy was adopted by the species to cope with the harsh physical conditions at the low elevation. The investment in rhizome decreased from low to high elevations, while the proportion of inflorescence mass increased, indicating that during the life history, the species shifts from predominant asexual reproduction to predominant sexual reproduction. This may be favourable for the species to colonize larger area, and to spread and persist at a meta_population level. Correlation analyses showed that sexual reproduction was inversely related to growth and asexual reproduction. However, it is difficult to determine the relationship between asexual reproduction and growth possibly because of the varied function of the corms of the species in different life history stages.
