Effect of Phytohormones on Adventitious Bud Differentiation from Bulb Scales of Oriental Lily Test-tube Plantlets

[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.

2-20 Synthesis of Uniform Sizes of Uranium Dioxide Kernels by Test-tube Experimrent in the Laboratory

In the 1970s and 1980s, United States Department of Energy conducted numerous studies on the fabrication of nuclear fuel particles using the internal gelation process[1], the internal gelation process is an advanced production process for nuclear fuels. Recently, we had used these lessons learned on the internal gelation process to prepare quantities of uranium dioxide kernels of uniform sizes. This paper discusses the whole efforts of experimental processes.

魔芋试管微球茎成熟度对其储藏及萌发率的影响

种子在母体植株上的干燥过程是种子完成从发育到萌发的一个重要转换点。魔芋组织培养中诱导试管微球茎过程中,在色泽上有1个明显的变化过程。本实验将这个过程分为3个阶段,对不同采收时期的微球茎进行储藏及萌发研究,结果表明,不同采收期的微球茎,储藏后的萌发率具有较大的差异,而且这种差异与魔芋种具有较大的相关性,这种差异与其保水性能有关。同时对试管微球茎收获度对储藏后萌发率影响的机制进行了初步的探讨。

花魔芋试管微球茎休眠解除的研究

为了研究花魔芋试管微球茎的萌发特性,设置了贮存时间、硫脲和赤霉素处理的试验。结果表明,贮存4～8个月的试管微球茎,在合适的温湿度条件下,萌发率低(10%～13%),休眠深浅不同;当赤霉素浓度达到100 mg.L-1或硫脲浓度为1%时,试管微球茎的萌发率最高,分别为73.5%和75.0%;

Effects of Sodium Chloride Stress on Growth of Sweet Potato Plantlets in vitro and Ion Content

[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.

JIVET Research on Texel Sheep

The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was conducted on 4-8-week old Texel sheep,and in-vivo ovum collection,in-vitro oocyte maturation and fertilization,and fertilized-embryo culture and transplantation were adopted to perform JIVET research on Texel sheep.The results showed that 296 available oocytes were obtained at an average of 74 oocytes per sheep;205 fertilized eggs were obtained at the 2-4 cell stage at an average of 51.25 eggs per sheep and a cleavage rate of 69.26%;and 66 of the fertilized eggs were transplanted to 11 receptor sheep.Six sheep were conceived at a conception rate of 54.55%,and 11 lambs were born.These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep.

Test-tube Baby Option

As China’s first test tube baby celebrates her 20th birthday,the country is moving to improve IVF procedures I feel just like a normal person,despite being a little bit hi-tech,"said China’s first test-tube baby Zheng Mengzhu at her birthday party in Beijing. Zheng,born on March 10,1988 in the No.3 Hospital affiliated to the Peking University of Medical Sciences,made a

Study on In-vitro Production Technology of Embryos from Young Dorper Sheep

This study was conducted to investigate the in-vitro production technology of embryos from young Dorper sheep, so as to provide technical support for the utilization of ovarian follicles in young Dorper sheep. Tests were conducted from the induction of Dorper sheep of 4 to 8 weeks old using follicle stimulating hormone(FSH) and pregnant horse serum(PMSG), collection of oocytes, in-vitro oocyte maturation-fertilization-zygote cultivation and 2-4-cell-stage fertilized ovum transfer. The results showed that 585 oocytes were collected from eight Dorper sheep at the age of 4 and 8 weeks, with an average of 73.13 oocytes/sheep. 346 of the 2-4-cell-stage fertilized eggs were obtained, whose cleavage rate was 59.15%. 77 in-vitro fertilized eggs at 2-4-cell stage were transplanted into 17 recipient sheep, seven of which were pregnant and gave birth to 13 "test-tube sheep" with a conception rate of 41.18%. It is indicated that the hormone induction technique, in-vitro oocyte maturation-fertilization-zygote cultivation technique and 2-4-cell-stage fertilized ovum transfer technique used in this study can serve as effective techniques for the in-vitro production of embryos from Dorper sheep of 4-8 weeks old.