Research of Seed Testa Structure and Storage Material of Peanut Germplasm with Different Resistance to A. flavus

There was an obvious relationship between seed testa structure, storage material and resistance to A. flavus of peanut. Results showed that seed testa of peanut germplasm with high resistance （HR） to A. flavus infection had thicker wax layer, integrated and tight epidermis layer, regular vascular tissue range. However, the seed testa of peanut germplasm with high sensitivity （HS） to A. flavus had the reverse results, and results of those with medium resistance （MR） to A. flavus lay in between, but changes of testa thickness were not significant among different resistance kinds. Results also showed that some seed storage materials were closely related with resistance potential to A. flavus. It seemed that varieties with higher resistance to A. flavus had higher oleic acid and protein content, lower linoleic acid and fat content. Content of palm acid, total sugar and VE did not show positive relationship with the resistance to A. flavus.

Photochemical synthesis of bimetallic Au-Ag nanoparticles with “core-shell” type structure by seed mediated catalytic growth

The colloidal Au core/Ag shell structure composite nanoparticles were synthesized in PEG-acetone solution by photochemical route. The monodispersed Au nanoparticles with average diameter of 3.9 nm were used as growth seeds. The optical property of colloids and the sizes of composite nanoparticles were characterized when the molar ratio of Au to Ag ranges from 4∶1 to 1∶4. The results show that a composite nanoparticle structure similar to strawberry shape is formed at the molar ratio of Au to Ag from 4∶1 to 1∶1; the composite nanoparticles consisting of a core of Au and shell of Ag were generated at the 1∶4 molar ratio, having a striking feature of forming (interconnected) network structure.

Physiological traits and anatomic structures of the seed for two short cotton season genotypes(Gossypium hirsutum L.) under water stress

Cotton(Gossypium hirsutum L.) is utilized commercially in the production of textile, clothing, and household goods. Its growth is affected by various environmental conditions such as soil, climate, and water supply. Irrigation is one of the most important factors for crop management. This research was designed with the aim of studying the biochemical and anatomical features of two genotypes of cottonseed(Latif and Golestan) in order to determine optimum irrigation. Protein, starch, gossypol gland, total soluble protein, starch content, and the anatomical structure of the cotton seeds were investigated. The data were analyzed using the SAS Software. The results showed that when rainfed, Golestan genotype seeds had a larger number of secretory cavities, soluble protein, and starch compared to Latif seeds. There was also a noticeable difference in the size of the seeds. Cellulosic cell walls and protein particles were also observed in the seed structure under induced water stress conditions. It can therefore be concluded that in response to water shortage, there was a noticeable change in the morphometric, anatomic, and biochemical features of the cottonseeds. It can be concluded that when rainfed, the Golestan genotype of cottonseed has more compatibility than the Latif genotype. In general, the application of different levels of irrigation showed that at moderate levels of irrigation, anatomical features appeared more normal. The observations indicate that at high levels of irrigation, some cells begin to dehisce due to osmotic stress, which results in a lack of accurate formation of tissue structures. The Golestan genotype is therefore the best suited for dryland farming.

Characterization of the Crystal Structure of Sesame Seed Cake Burned by Nd: YAG Laser

This paper reports obtaining of useful and high-value materials from sesame seed cake (SSC). For this purpose, SSC sample was burned for 30 s using Nd: YAG laser with output power 60 W. The products of this process and non-burned SSC were characterized by X-ray diffractometer (XRD), energy dispersive x-ray (EDX) and Fourier transform infrared (FTIR) so as to investigate its crystal structure and chemical components. XRD results of the SSC before burning process showed amorphous silica, rhombohedral phase of carbon, monoclinic phase of aluminum chloride, the hexagonal phase of moissanite-4H, (yellow, black) and hexagonal phase of graphite-2H, C (black). While the results of the burned SSC sample showed that the burning process using the power of Nd: YAG laser cased in appearing of crystalline hexagonal phase for silica and Carbon Nitride and converting the rhombohedral phase of Carbon into hexagonal phase. FTIR showed a number of absorbance peaks assigned to silica.

Study of Viability and Seed Structure of Shallot

In Indonesia, shallot cultivation finds difficulties to produce high viability seed. That low viability is caused by the abnormality of seed＇s structure. Normal shallot seeds have seed structure that consists of： testa, endosperm, and embryo. Normal embryo has curved shape which it has one cotyledon in endosperm. This research purpose is to find shallot viability and structure that is cultivated in tropic area, especially in Indonesia. This research is a deep observation research about water level, seeds viability and seeds structure monitoring by using microscope. To make a clearance about seeds structure composition, seeds are mixed with tetrazolium salt. Monitoring result shows that seed water level at harvest time is 18%, laid away at one month age is 13%, two months age is 12% and three months age is 10%. Seed viability at harvest until twenty nine days, seed that is germinated around 8%. At two months viability seed laid age until twenty two days is 36.6%. Meanwhile three months age laid viability test is done with germination boost compounding shows that IAA is able to fasten germination. At IAA treatment viability seeds are 17.25% that is followed with GA3 treatment around 13%. Another boosting substance that it＇s viability is only below 4%. Low viability is also proved by the seed structure. The result shows that mostly, the seeds has abnormal structure.

Synthesis and Characterization of Fluorine-containing Polyacrylate Emulsion with Core-Shell Structure

A fluorine-containing polyacrylate copolymer emulsion was synthesized by a seed emulsion polymerization method, in which methyl methacrylate （MMA） and butyl acrylate （BA） were used as main monomers and hexafluorobutyl methacrylate （HFMA） as fluorine-containing monomer. The structure and properties were characterized by Fourier transform infrared spectrum （FT-IR）, transmission electron microscopy （TEM）, particle size analysis, X-ray photoelectron spectroscopy （XPS）, contact angle （CA）, differential scanning calorimetry （DSC） and thermogravimetry （TG） analysis. The FTIR and TEM results showed that HFMA was effectively involved in the emulsion copolymerization, and the formed emulsion particles had a core-shell structure and a narrow particle size distribution. XPS and CA analysis revealed that a gradient concentration of fluorine existed in the depth profile of fluorine-containing emulsion film which was richer in fluorine and more hydrophobic in one side. DSC and TG analysis also showed that a clear core-shell structure existed in the fluorine-containing emulsion particles, and their film showed higher thermal stability than that of fluorine-free emulsion.

Abnormal Structure of Embryo Sac in Autotetraploid Rice

The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice （Oryza sativa L.）, including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy （WCLSM）. Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and ＇double set＇ of embryo sacs. The frequency of abnormal embryo sac in japonica （26.6%） was higher than that in indica （19.34%）. In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties.

Current patterns of plant diversity and phylogenetic structure on the Kunlun Mountains

Large-scale patterns of biodiversity and the underlying mechanisms that regulate these patterns are central topics in biogeography and macroecology.The Qinghai-Tibet Plateau serves as a natural laboratory for studying these issues.However,most previous studies have focused on the entire Qinghai-Tibet Plateau,leaving independent physical geographic subunits in the region less well understood.We studied the current plant diversity of the Kunlun Mountains,an independent physical geographic subunit located in northwestern China on the northern edge of the Qinghai-Tibet Plateau.We integrated measures of species distribution,geological history,and phylogeography,and analyzed the taxonomic richness,phylogenetic diversity,and community phylogenetic structure of the current plant diversity in the area.The distribution patterns of 1911 seed plants showed that species were distributed mainly in the eastern regions of the Kunlun Mountains.The taxonomic richness,phylogenetic diversity,and genera richness showed that the eastern regions of the Kunlun Mountains should be the priority area of biodiversity conservation,particularly the southeastern regions.The proportion of Chinese endemic species inhabiting the Kunlun Mountains and their floristic similarity may indicate that the current patterns of species diversity were favored via species colonization.The Hengduan Mountains,a biodiversity hotspot,is likely the largest source of species colonization of the Kunlun Mountains after the Quaternary.The net relatedness index indicated that 20 of the 28 communities examined were phylo-genetically dispersed,while the remaining communities were phylogenetically clustered.The nearest taxon index indicated that 27 of the 28 communities were phylogenetically clustered.These results suggest that species colonization and habitat filtering may have contributed to the current plant diversity of the Kunlun Mountains via ecological and evolutionary processes,and habitat filtering may play an important role in this ecological process.

Magnetic properties and structure of Ni_80Fe_20/Ni_48Fe_12Cr_40 bilayer films deposited on SiO_2/Si(100) by electron beam evaporation

Ni80Fe20/Ni48Fe12Cr40 bilayer films and Ni80Fe20 monolayer films were deposited at room temperature on SiO2/Si（100） substrates by electron beam evaporation. The influence of the thickness of the Ni48Fe12Cr40 underlayer on the structure, magnetization, and magnetoresistance of the Ni80Fe20/Ni48Fe12Cr40 bilayer film was investigated. The thickness of the Ni48Fe12Cr40 layer varied from about 1 nm to 18 nm while the Ni80Fe20 layer thickness was fixed at 45 nm. For the as-deposited bilayer films the introducing of the Ni48Fe12Cr40 underlayer promotes both the （111） texture and grain growth in the Ni80Fe20 layer. The Ni48Fe12Cr40 underlayer has no significant influence on the magnetic moment of the Ni80Fe20/Ni48Fe12Cr40 bilayer film. However, the coercivity of the bilayer film changes with the thickness of the Ni48Fe12Cr40 undedayer. The optimum thickness of the Ni48Fe12Cr40 underlayer for improving the anisotropic magnetoresistance effect of the Ni80Fe20/Ni48Fe12Cr40 bilayer film is about 5 nm. With a decrease in temperature from 300 K to 81 K, the anisotropic magnetoresistance ratio of the Ni80Fe20 （45 nm）/Ni48Fe12Cr40 （5 nm） bilayer film increases linearly from 2.1% to 4.8% compared with that of the Ni80Fe20 monolayer film from 1.7% to 4.0%.

Preparation of Core-shell Structured Particles and Their Nucleation in Polyester:I. Preparation of Monodisperse SiO_2/PS Core-shell Composite Particles

To enhance the nucleation and crystallization properties of polyester (e.g., polyethylene terephthalate, PET), core-shell structured particles are used to improve these properties by controlling the inorganic dispersion properties in the polymers. In the paper, monodisperse particles of silica/polystyrene (PS) are prepared with both dispersion and emulsion polymerization techniques. The monodisperse silicon dioxide particles are first prepared with the seed growth method and modified by the coupling agents. Silica is properly modified with KH-570, and its size deviation is 3.0% or so. The modified silica then reacts with the mixture of ethanol, water medium, and monomer of styrene under dispersion polymerization. Results show that the dispersion polymerization technique is more suitable for monodisperse core-shell SiO2/PS particles than that of the emulsion. The morphology and molecular structure of the core-shell particles are investigated with the transmission electron microscope (TEM), and fourier transform infra-red spectroscopy (FTIR). The results show that the modified silica particles are successfully encapsulated with polystyrene. The average number of silica particles encapsulated into each polystyrene sphere decreases when the size of silica particles increases from 50 nm to 600 nm, and will approach one when the silica is greater than 380nm in size. The mass ratio for silica/PS particles in emulsion polymerization is 4.7/1, lower than that of 6.8/1 for dispersion polymerization, which is the first reported optimized data for preparing the similar monodisperse composite particles. Thus, the PS shell in the former is thinner than that in the latter.

Genetic diversity and structure of Drimys brasiliensis in southern Brazil:insights for conservation

Population genetics studies are widely recognized for generating useful knowledge for biodiversity conservation.To date,however,little is known about the levels and distribution of genetic diversity of Drymis brasiliensis(Miers LC),a tree species from the Atlantic Rainforest.Therefore,in this study,we investigated how genetic diversity is distributed within and among populations of D.brasiliensis from southern Brazil using allozyme markers to genotype reproductive trees(8 populations)and seedlings(3 populations).Furthermore,in two populations,we established two permanent plots(5.1 and 1 ha)to analyze fine-scale genetic structure(FSGS).Studied populations presented low levels of genetic diversity(reproductive=0.085;seedlings=0.054)and high fixation indexes(reproductive=0.396;seedlings=0.231).Genetic divergence among populations was equal to 0.05,which is significant,signaling that few populations can conserve large portions of the species total genetic diversity.FSGS was only detected for one population,when reproductive individuals were separated by less than40 m.Low genetic diversity combined with high fixation indexes clearly signal a risk of losing diversity.Therefore,conservation efforts should be aimed at enhancing gene flow within the studied populations.

Polycrystalline ZSM-5 Aggregates Induced by Seed and Catalytic Performance in Methanol to Hydrocarbon

Synthesis of ZSM-5 zeolite typically utilizes small molecule polyamines or quaternary ammonium salts as organic structure guiding agent(OSDA).By contrast,the OSDA-free hydrothermal synthesis system eliminates the use of organic templates and the subsequent calcination procedure.This not only reduces the cost of synthesis,but also prevents environmental pollution from the combustion of organic templates,representing an eco-friendly approach.Despite this,literature suggests that even so-called template-free synthesis systems often involve trace amount of organic substances like alcohol.In the present work,a calcined commercial ZSM-5 zeolite was served as seed,with sodium aluminate as aluminum source and silica sol as silicon source,ensuring an entirely template-free synthesis system.Polycrystalline ZSM-5 aggregates consisted of rod-like nanocrystals were successfully prepared in the completely OSDA-free system.Effects of the Si/Al ratio in ZSM-5 seed,dosage and crystallization conditions such as crystallization temperature and crystallization time on ZSM-5 synthesis were investigated.The results show that a highly crystallinity ZSM-5 aggregate consisting of primary nano-sized crystals less than 100 nm is produced from a gel precursor with 5.6%(in mass)seed after hydrothermal treatment for 48 h.Furthermore,the Si/Al ratio in ZSM-5 seed has little effect on the topological structure and pore structure of the synthesized samples.However,the seeds with a low Si/Al ratio facilitate faster crystallization of zeolite and enhance the acidity,especially the strong acid centers,of the catalyst.The catalytic performance of the synthesized polycrystalline ZSM-5 was evaluated during dehydration of methanol and compared with a commercial reference ZSM-5r.The results exhibit that as compared with the reference catalyst,the fabricated sample has a longer catalytic lifetime(16 h vs 8 h)attributed to its hierarchical pores derived from the loosely packed primary nanoparticles.Additionally,the prepared polycrystalline catalyst also exhibits a higher aromatics selectivity(28.1%-29.8%vs 26.5%).

Deletion of a 1,049 bp sequence from the 5′UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame

Sesame is a labor intensive crop with limited mechanized harvesting mainly due to the seed shattering(SS)trait.In this study,we performed a genetic analysis of the seed-shattering resistance(SR)trait with a SR sesame mutant 12M07.Unlike the SS type,the parenchyma cells in the abscission zone of the 12M07 mutant are arranged loosely but adhere to the seed coat.Inheritance analysis of six generations derived from 12M07(SR)×Xiangcheng Dazibai(SS)showed that the SR trait is recessive and controlled by a single gene pair.Association mapping of the F2population with 888,619 variants(single-nucleotide polymorphisms(SNPs)and insertion-deletion(InDels))and 31,884 structural variations(SVs)determined that only SV12002 in the 5′upstream region of gene Sindi0765000(named SiHEC3)in Chr.3 was significantly associated with the SR trait.SiHEC3 encodes the bHLH transcription factor.A 1,049 bp deletion occurred in the 5′UTR of Sihec3 in 12M07.SiHEC3 is mainly expressed in developing placental tissues,with the expression peaking in capsules at 45 days after pollination.A dual-luciferase reporter assay in tobacco confirmed that the promoter activity of Sihec3 was reduced because of the deletion of the 1,049 bp promoter sequence.Protein–protein interaction network analysis showed that HEC3 is co-expressed with nine key proteins,such as SHATTERPROOF1(SHP1)and SEEDSTICK(STK)which participate in the secondary wall biosynthesis of the abscission layer in plants.The findings of this study show the important function of Sihec3corresponding with the SR trait and supply the genetic information for breeding new varieties that are amenable to mechanized harvesting in sesame and other crops.

Changes in structure,functional properties and volatile compounds of Cinnamomum camphora seed kernel protein by Maillard reaction

In this study,Cinnamomum camphora seed kernel protein isolate(CPI)-dextran(DX)model system was used to investigate the impacts of reaction temperatures(70-100℃)and times(1-4 h)on the structure,functional properties and volatile compounds of Maillard reaction products(MRPs).The formation of MRPs was confirmed by SDS-PAGE,ultraviolet absorption and degree of glycation.Fourier transform infrared(FTIR)and circular dichroism(CD)spectroscopy showed that the secondary structure of MRPs tended to be more regular than native CPI.Moreover,MRPs had a lower free sulfhydryl group content and higher surface hydrophobicity than native CPI.Fluorescence spectra revealed that the tryptophan residues of CPI were transferred to a more hydrophobic microenvironment.The solubility and emulsifying stability of CPI were decreased,while the foaming activity,foaming stability,emulsion stability and apparent viscosity of CPI were significantly improved(p<0.05)after Maillard reaction.Furthermore,MRPs exhibited significantly improved(p<0.05)thermal stability and ABTS^(+)•radical scavenging activity than native CPI,while the DPPH+radical scavenging activity and ferric reducing power were decreased.In addition,volatile compounds,including aldehydes,ketones,benzenes and esters,were identified in MRPs.These results provide a theoretical guidance for future studies on CPI-DX conjugates.

基于SEED波形文件的运行率统计

介绍了基于SEED波形文件运行率计算的技术和实现方法,介绍了SEED文件中标识时间的特殊掩码、时间片的具体格式和程序的实现,并给出了与其相关的部分源代码。

Phenomena in late period of seeded precipitation of sodium aluminate solution

Aiming at seeded precipitation of aluminate solution with high caustic ratio(αk>2.4), corresponding to the late period of seeded precipitation, the influence of different types of seed on precipitation ratio was explained with respect to solution structure in the interface of seed and the evolution of Al(OH)3 growth units in this layer. The effects of solid content and seed size on agglomeration were determined by calculating the particle number of product. The results imply that the solution structure in the interface of seed imposes a notable significance on the process in the late period of seeded precipitation. Agglomeration still exists in this period. However, the agglomeration bodies break in the case of prolonging precipitation due to the mechanical effect, which results in the increase of particle number.

Separation,structural identification and antibacterial activity of pectin oligosaccharides derived from seed melon

In this study,the pectin of waste seed melon peel in Xinjiang was used as raw material to prepare seed melon pectin oligosaccharide(Sm-POS).The separation and purification of Sm-POS were carried out by HPD-750 macroporous resin chromatography column and gel chromatography to further study the molecular structure of its active component with antibacterial effect by FT-IR,NMR,and LC/MS.The antibacterial activity and mechanism of the Sm-POS active component were investigated by inhibition zone experiment,minimum inhibitory concentration determination,cell growth curve determination,scanning electron microscopy,and flow cytometry analysis.Results showed that Sm-POS-2 was an oligosaccharide composed of two galacturonic acids,one glucose,one arabinose,and one xylose.It showed good antibacterial activity and suppressed the growth of E.coli and S.aureus at minimum inhibitory concentrations(MIC)of 25.0 and 50.0 g/L,respectively.The inhibitory effect of Sm-POS on E.coli and S.aureus was mainly due to cell membrane contraction or rupture,leading to the apoptosis or necrosis of bacterial cells.

A New Pseudo Sesquiterpenoid from the Seeds of Koelreuteria paniculata

From the seeds of medicinal plant Koelreuteria paniculata, a new pseudo sesquiter- penoid with novel skeleton namely paniculoid 1 was isolated. The structure of 1 was established on the basis of extensive 2D NMR spectroscopy in conjugation with MS and IR spectral analysis.

气吸双行错置式玉米密植精量排种器设计与试验

针对大豆-玉米复合密植播种模式下传统气吸式排种器单行种盘高转速作业导致充种时间短、气流稳定性差,难以实现高速精量密植播种的问题,设计了一种气吸双行错置式玉米密植精量排种器,阐述了排种器结构与工作原理,对其工作过程及关键部件进行理论分析,构建充种和投种环节的种子力学模型,确定排种盘内外环型孔排布、投种轮、气室等关键结构参数,并开展单、双气道内负压分布、型孔内气流场特性分析,基于DEM-CFD耦合方法对排种器的排种过程进行仿真分析,以作业速度、气室结构和负压为试验因素,充种合格指数、重充指数和漏充指数为评价指标,优选出最优气室结构。通过台架试验开展不同气吸式排种器排种性能对比试验。试验结果表明,在作业速度为5~10 km/h的高速密植工况下,气吸双行错置式密植精量排种器排种合格指数均大于88.7%,且作业速度为10 km/h时,相较于常用单圆环气吸式排种器合格指数提高5.5个百分点,漏播指数降低5.6个百分点;田间试验结果表明,在作业速度为5 km/h下,播种合格指数为95.7%,重播指数为1.6%,漏播指数为2.8%。提出的气吸双行错置式玉米密植精量排种器在高速作业时拥有良好的排种性能,能够满足大豆-玉米高速精量密植播种要求。

油菜高速机械离心式集排器设计与试验

针对传统油菜机械离心式集排器高速作业时供种及投种能力不足,导致排种量与作业速度不匹配、排种性能不稳定等问题,设计了一种被动式供种、“圆孔+渐变孔柱”组合式型孔投种的油菜高速机械离心式集排器。构建了供种、投种过程中的力学模型,分析确定了影响排种性能的关键结构参数;利用EDEM离散元仿真开展了供种调节高度对供种速率调节量影响的试验,结果表明当供种调节高度为3~8 mm时,供种速率在64.95~357.54 g/min范围内可调;采用两因素三水平正交试验分析了限种套筒下摆高度及倾角对初始种量、临界转速及环状种层最大高度的影响。通过台架试验确定了较优限种套筒结构参数,结合高速摄影对比了5种型孔结构下动锥体转速与排种量关系,确定最优型孔结构为“圆孔+渐变孔柱”组合式型孔。较优参数组合集排器排种性能验证试验结果表明:当转速为115~205 r/min时,排种速率为60.96~355.76 g/min,油菜各行排量一致性变异系数均低于5.2%,总排量稳定性变异系数均低于1.3%,破损率低于0.5%,满足作业速度6~12 km/h时的排种量要求。田间试验结果表明,当机组作业速度为7.89、11.98 km/h时,油菜各行植株均匀性变异系数低于11%,种植密度为43~58株/m^(2),满足油菜精量播种要求。