Analysis and Study on the Change of the Observed Geo-electric Field Data at Lhasa Geomagnetic Station before and after the Nepal MS8.1 Earthquake

In this paper,the main purpose is to analyze and research the characteristics of the geoelectric field observed data with a long time span and large amplitude abnormal change,at the Lhasa geomagnetic station( hereafter referred to as "Lhasa station "),before and after the Nepal M_S8. 1 strong earthquake,which occurred on April 25,2015. Based on the observation conditions,the observation system,and the observed data of Lhasa station preliminary discussed,the main characteristics of the abnormal change and evolution process are analyzed and studied,using the following two methods; the "synthesis energy accumulation"and the "power as MSA spectrum"analysis,from the two aspects of the"Time Domain"and"Frequency Domain. "The results show that the abnormal change of the geo-electric field observation of Lhasa station experienced a development stage following the process of "trend change- disturbance change- earthquake period-recovery period",and an evolution process of "low frequency change- high frequency change- smooth change- high frequency change ",before and after the Nepal M_S8. 1strong earthquake. Comprehensive analysis shows that the variation characteristics and evolution process of the geo-electric field at Lhasa station are basically consistent with the results of the relevant mechanism and phenomenon research. So far,this is valuable information with certain objectivity,which is typical and representative to reflect the whole process of the gestation, occurrence and complete development of such strongearthquakes.

Sentinel-1 In SAR observations and time-series analysis of co-and postseismic deformation mechanisms of the 2021 Mw 5.8 Bandar Ganaveh Earthquake,Southern Iran

In the past two decades,because of the significant increase in the availability of differential interferometry from synthetic aperture radar and GPS data,spaceborne geodesy has been widely employed to determine the co-seismic displacement field of earthquakes.On April 18,2021,a moderate earthquake(Mw 5.8)occurred east of Bandar Ganaveh,southern Iran,followed by intensive seismic activity and aftershocks of various magnitudes.We use two-pass D-InSAR and Small Baseline Inversion techniques via the LiCSBAS suite to study the coseismic displacement and monitor the four-month post-seismic deformation of the Bandar Ganaveh earthquake,as well as constrain the fault geometry of the co-seismic faulting mechanism during the seismic sequence.Analyses show that the co-and postseismic deformation are distributed in relatively shallow depths along with an NW-SE striking and NE dipping complex reverse/thrust fault branches of the Zagros Mountain Front Fault,complying with the main trend of the Zagros structures.The average cumulative displacements were obtained from-137.5 to+113.3 mm/yr in the SW and NE blocks of the Mountain Front Fault,respectively.The received maximum uplift amount is approximately consistent with the overall orogen-normal shortening component of the Arabian-Eurasian convergence in the Zagros region.No surface ruptures were associated with the seismic source;therefore,we propose a shallow blind thrust/reverse fault(depth~10 km)connected to the deeper basal decollement fault within a complex tectonic zone,emphasizing the thin-skinned tectonics.

The Co-seismic Response of Underground Fluid in Yunnan to the Nepal MS8.1 Earthquake

In this paper,statistics are taken on the co-seismic response of underground fluid in Yunnan to the Nepal M_S8. 1 earthquake,and the co-seismic response characteristics of the water level and water temperature are analyzed and summarized with the digital data. The results show that the Nepal M_S8. 1 earthquake had greater impact on the Yunnan region,and the macro and micro dynamics of fluids showed significant co-seismic response. The earthquake recording capacity of water level and temperature measurement is significantly higher than that of water radon and water quality to this large earthquake; the maximum amplitude and duration of co-seismic response of water level and water temperature vary greatly in different wells. The changing forms are dominated by fluctuation and step rise in water level,and a rising or falling restoration in water temperature. From the records of the main shock and the maximum strong aftershock,we can see that the greater magnitude of earthquake,the higher ratio of the occurrence of co-seismic response,and in the same well,the larger the response amplitude,as well as the longer the duration. The amplitude and duration of co-seismic response recorded by different instruments in a same well are different. Water temperature co-seismic response almost occurred in wells with water level response,indicating that the well water level and water temperature are closely related in co-seismic response,and the well water temperature seismic response was caused mainly by well water level seismic response.

1,8-桉叶油素干预大鼠实验性牙周炎模型的炎症反应

背景:研究表明,天然植物精油提取物1,8-桉叶油素具有抗炎、抗氧化、抗菌、抗肿瘤等多种药理作用,在多种疾病中表现出抗炎作用。目的:探讨1,8-桉叶油素对大鼠实验性牙周炎模型炎症反应的影响。方法:按照完全随机数字表法将30只SD大鼠随机分为正常对照组、牙周炎对照组和1,8-桉叶油素组,每组10只。牙周炎对照组和1,8-桉叶油素组利用正畸钢丝结扎法构建实验性牙周炎模型。造模8周后,正常对照组和牙周炎对照组大鼠颊、腭侧牙周袋内均注射生理盐水,1,8-桉叶油素组大鼠颊、腭侧牙周袋内注射1,8-桉叶油素溶液,2次/d,连续给药4周。给药结束后,进行牙周临床指标检测、牙周组织学评估、血清炎症因子水平检测及牙龈组织中炎症因子m RNA与蛋白表达检测。结果与结论:①与正常对照组比较,牙周炎对照组大鼠牙龈出血指数、牙周探诊深度增加(P<0.05),血清中白细胞介素1β、肿瘤坏死因子α、白细胞介素6水平升高(P<0.05),血清中白细胞介素10水平降低(P<0.05),牙龈组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素6mRNA与蛋白表达均升高(P<0.05),牙龈组织中白细胞介素10 mRNA与蛋白表达均降低(P<0.05);苏木精-伊红染色结果显示,牙周炎对照组大鼠牙周组织炎症明显。②与牙周炎对照组比较,1,8-桉叶油素组大鼠牙龈出血指数、牙周探诊深度减少(P<0.05),血清中白细胞介素1β、肿瘤坏死因子α、白细胞介素6水平降低(P<0.05),血清中白细胞介素10水平升高(P<0.05),牙龈组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素6 mRNA与蛋白表达均降低(P<0.05),牙龈组织中白细胞介素10 mRNA与蛋白表达均升高(P<0.05);苏木精-伊红染色结果显示,1,8-桉叶油素组大鼠牙周组织炎症明显减轻。结果表明,1,8-桉叶油素治疗可减轻大鼠实验性牙周炎模型的炎症反应。

MMSA-8、MMSA-1在急性髓系白血病患者骨髓单个核细胞中的水平及临床意义

目的:分析多发性骨髓瘤相关抗原(MMSA)-8、MMSA-1在急性髓系白血病患者骨髓单个核细胞中的水平及MMSA-8、MMSA-1在急性髓系白血病中的临床意义。方法:选取2021年1月~2022年12月苏州大学附属第一医院收治的急性髓系白血病30例患者为观察组,再选择同期被诊断为单纯缺铁性贫血的患者25例为对照组,比较两组MMSA-8和MMSA-1 mRNA的水平差异。对观察组患者进行随访1年,分析急性髓系白血病患者1年生存情况,根据随访预后疗效将其分为良好组(n=25)和不良组(n=5)。比较不同预后急性髓系白血病患者MMSA-8和MMSA-1 mRNA的水平差异。运用受试者工作特征(ROC)曲线分析MMSA-8和MMSA-1 mRNA水平预测急性髓系白血病的效能。结果:观察组MMSA-8、MMSA-1 mRNA水平明显高于对照组(P<0.05)。30例急性髓系白血病患者术后均随访1年,预后良好25例,预后不良5例,良好率为83.33%(25/30)。不良组的MMSA-8、MMSA-1 mRNA水平明显高于良好组(P<0.05)。ROC曲线分析结果显示,MMSA-8、MMSA-1 mRNA水平与急性髓系白血病患者的曲线下面积为0.880、0.840,95%CI为0.709~0.969、0.661~0.948,预测效能较好。结论:MMSA-8和MMSA-1 mRNA的水平在急性髓系白血病中呈高水平表达,在预测急性髓系白血病的效能较好,临床可借助该两项指标预测急性髓系白血病的病程以及预后。

Surface rupture zone of the 1303 Hongtong M=8 earthquake, Shanxi Province

Based on the latest displacement of Huoshan piedmont fault, Mianshan west-side fault and Taigu fault obtained from the beginning of 1990s up to the present, the characteristics of distribution and displacement of surface rup-ture zone of the 1303 Hongtong M = 8 earthquake, Shanxi Province are synthesized and discussed in the paper. If Taigu fault, Mianshan west-side fault and Huoshan piedmont fault were contemporarily active during the 1303 Hongtong M = 8 earthquake, the surface rupture zone would be 160 km long and could be divided into 3 segments, that is, the 50-km-long Huoshan piedmont fault segment, 35-km-long Mianshan west-side fault segment and 70-km-long Taigu fault segment, respectively. Among them, there exist 4 km and 8 km step regions. The surface rupture zone exhibits right-lateral features. The displacements of northern and central segments are respectively 6~7 m and the southern segment has the maximum displacement of 10 m. The single basin-boundary fault of Shanxi fault-depression system usually corresponds to M 7 earthquake, while this great earthquake (M = 8) broke through the obstacle between two basins. It shows that the surface rupture scale of great earthquake is changeable.

Near-field surface deformation during the April 20,2013,Ms7.0 Lushan earthquake measured by 1-Hz GNSS

The April 20, 2013, Ms7.0 Lushan earthquake was successfully recorded by closely spaced Continuous Global Positioning System （CGPS） stations owned by the Crustal Movement Observation Network of Chi- na （CMONC）. The 1-Hz GNSS data from eight CGPS stations, which are located between 30 km and 200 km from the hypocenter, were processed within quasi-real-time. The near-field surface deformation indicated the following characteristics ： the near-field movements were limited to several centimeters ; the peak of the deformation wave was significantly larger than the static permanent offset; at the beginning of the event, the north wall of the fault moved to the southeast as the south wall moved to the southwest ; station SCTQ, which was the closest station to the hypocenter at 30 km, had the largest static permanent displacement of 2 cm; the peaks of the deformation waves were 1.5 cm, 5 cm and 3 cm, to the east, the south and vertically upward, respectively ; and the peaks of velocity and acceleration, derived from the deformation, were 3.4 cm/s and 5.3 cm/s^2,respectively.

The GIS and analysis of earthquake damage distribution of the 1303 Hongtong M=8 earthquake

The geography information system of the 1303 Hongtong M=8 earthquake has been established. Using the spatial analysis function of GIS, the spatial distribution characteristics of damage and isoseismal of the earthquake are studied. By comparing with the standard earthquake intensity attenuation relationship, the abnormal damage dis-tribution of the earthquake is found, so the relationship of the abnormal distribution with tectonics, site condition and basin are analyzed. In this paper, the influence on the ground motion generated by earthquake source and the underground structures near source also are studied. The influence on seismic zonation, anti-earthquake design, earthquake prediction and earthquake emergency responding produced by the abnormal density distribution are discussed.

Improvement of instantaneous positioning by spatial stacking and modified sidereal filtering methods with application to the 2008 Ms8.0 Wenchuan earthquake

We obtained several displacement time series from the Sichuan permanent GPS net and processed the 1-Hz data observed during a few days before the 2008 Ms8.0 Wenchuan earthquake by double-difference instantaneous positioning technique. We filtered the data by the spatial stacking and the modified sidereal filte- ring methods to reduce correlation bias in space and time. The results indicate that these methods can improve the precision significantly.

Study on Focal Mechanism Changing Characteristics of the 2013 Zogang-Markam Ms6. 1 Earthquake Sequence

The M_S6. 1 earthquake was a foreshock-mainshock-aftershock type which occurred in the boundary region between Zogang and Markam counties on August 12,2013. Within 9hours before the main shock seven earthquakes of greater than M_L2. 0 occurred,with a maximum of M_L4. 7. In this paper,the earthquake focal mechanism changing process of the Zogang-Markam M_S6. 1 earthquake sequence is studied by calculating the correlation coefficient of body wave spectral amplitudes,and the result shows that the correlation coefficients of spectral amplitude of foreshocks present high value fluctuation with an average value of 0. 86,which shows that the focal mechanism of foreshocks are similar;and the correlation coefficients of spectral amplitude of aftershocks present low value,which shows that the possibility of a large earthquake is not high after a time.

New Insight into the Surface Rupture Parameters of the Kunlun Mountains Pass M_S8.1 Earthquake

Field observation shows that the surface rupture of the Kunlun Mountains Pass M_S 8.1 earthquake is about 426km long, and the maximum sinistral displacement is about 6m. Distribution of horizontal displacement along the surface ruptures is markedly controlled by fault structure. The rupture length of this earthquake is significantly longer than statistic value. In this paper, using the method of “ultimate linear strain", we discussed the independency and integrality of the whole rupture zone and rupture segments of the Kunlun Mountains Pass earthquake by comparing with some large earthquakes on strike-slip faults on the Chinese continent. The conclusion is that the Kunlun Mountains Pass earthquake consists of successively triggered multiple earthquake events, other than a single earthquake event.

Analysis on Characteristics of Seismic Damage of the Nepal M_S8.1 Earthquake in the Tibet Area of China

On April 25,2015,a M_S8. 1 earthquake occurred in Nepal. In the Tibet area of China,this earthquake caused heavy casualties and damage to housing,roads,communications,other lifeline engineering, water conservancy and other infrastructure. This paper introduces the basic situation of the earthquake,and based on the investigation and assessment of seismic intensity,the damage of the disaster area is analyzed,and building types and damage to the lifeline systems and various industries are given. Through the analysis of the characteristics of the earthquake disaster,this paper points out the existing problems in seismic fortification,and finally puts forward proposals for the prevention and control of earthquake geological disasters, scientific planning for the restoration and reconstruction,strengthening earthquake prevention and disaster reduction propaganda,improving the awareness of earthquake preparedness in the agricultural and pastoral areas,strengthening the guidance and supervision of housing construction in rural areas to reduce the casualties and losses,and promoting the harmonious development of economy in Tibet.

Correlated Features of Horizontal Movement-Deformation on the North and East Margins of the Qinghai-Xizang Block before the Kunlun Earthquake with M_S=8.1

The high-precision GPS data observed from the northeast margin of the Qinghai-Xizang （Tibet） block and the Sichuan-Yunnan GPS monitoring areas in 1991 （1993）, 1999 and 2001 revealed that： before the Kunlun earthquake with Ms =8.1 on November 14, 2001, the dynamic variation features of horizontal movement-deformation field in the north and east marginal tectonic areas of the Qinghai-Xizang （Tibet） block had some correlated features. That is to say, under the general background of inherited movement, the movement intensifies in the two areas weakened synchronously and the state of deformation changed when the great earthquake was impending. Analysis and study in connection with geological structures showed that before the Kunlun Ms8.1 earthquake, the correlated variations of movement-deformation on the boundaries of Qinghai-Xizang （Tibet） block were related to the disturbing stress field caused by the extensive and rapid stress-strain accumulation in the late stage of large earthquake preparation. Owing to the occurrence of large earthquake inside the block, the release of large amount of strain energy, and the adjustment of tectonic stress field, in relevant structural positions （especially zones not penetrated by historical strong earthquake ruptures） in boundary zones where larger amount of strain energy was accumulated, stress-strain may be further accumulated or else released through rupture.

Research on Stress Drops and the Focal Mechanisms of the Xinyuan- Hejing ML6.8 Earthquake Sequences

Based on the digital waveform data recorded by Xinjiang Digital Seismic Network for the Xinyuan-Hejing M_L6.8 earthquake sequences of June 30,2012,this paper analyzes the stress drops of earthquake sequences and the correlation coefficients of focal mechanisms significant for strong aftershocks.Firstly,the source parameters of the Xinyuan-Hejing M_L6.8 earthquake sequences are obtained by applying the spectrum analysis and the Brunes source model.Then,the correlation coefficients of spectral amplitudes are calculated using the low-frequency spectral amplitude recorded by the same station for the different events.Finally,based on the results of the correlation coefficients of spectral amplitudes,the events with similar focal mechanisms are grouped using the clustering method.The results show that:(1)The stress drop values show a steady trend in the aftershock sequence calm period and the stress drop values show a rise-fall in strong aftershocks.(2)The moving average correlation coefficient of amplitude spectrum begins to spread after the main shock.It shows that the correlation decreases between the main shock and the aftershocks in mechanisms.(3)The results of focal mechanism groups show that the earthquake sequences are mainly strike slips.The stress distribution of the main pressure axis is nearly NS,which is the same as the structural stress field.(4)The magnitude and mechanism show that there is an agreement before the strong aftershock,which shows that the regional stress field is enhanced.

急性胆囊炎患者胆囊切除术后血清CCK-8、TREM1水平与发生感染的关系

目的分析急性胆囊炎(AC)患者胆囊切除术后血清胆囊收缩素-8(CCK-8)、髓系细胞触发受体1(TREM1)水平与发生感染的关系。方法将该院2020年12月至2022年12月收治的70例胆囊切除术后发生感染的AC患者纳入研究组,66例胆囊切除术后未发生感染的AC患者纳入对照组。采用酶联免疫吸附试验检测血清CCK-8、TREM1水平。采用Pearson相关分析胆囊切除术后发生感染AC患者血清中CCK-8、TREM1水平与炎症因子水平的相关性。采用受试者工作特征(ROC)曲线分析血清CCK-8、TREM1水平对AC患者胆囊切除术后发生感染的诊断价值。采用多因素Logistic回归分析AC患者胆囊切除术后感染的影响因素。结果研究组与对照组有胆囊结石、胆囊周边积液比例比较,差异均有统计学意义(P<0.05)。与对照组比较,研究组血清CCK-8水平明显降低,TREM1水平明显升高,差异均有统计学意义(P<0.05)。研究组C反应蛋白(CRP)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)水平明显高于对照组,差异均有统计学意义(P<0.05)。胆囊切除术后发生感染的AC患者血清CCK-8水平与CRP、IL-8、TNF-α水平均呈负相关(P<0.05),TREM1水平与CRP、IL-8、TNF-α水平均呈正相关(P<0.05)。ROC曲线分析显示,血清CCK-8与TREM1联合检测诊断AC患者胆囊切除术后发生感染的曲线下面积(AUC)明显大于CCK-8、TREM1单独检测的AUC(Z=5.703,P<0.001;Z=4.584,P<0.001)。有胆囊结石、胆囊周边积液及血清CCK-8水平降低、血清TREM1水平升高均为AC患者胆囊切除术后发生感染的危险因素(P<0.05)。结论胆囊切除术后发生感染的AC患者血清CCK-8水平降低,TREM1水平升高,二者联合检测能够提高对AC患者胆囊切除术后发生感染的诊断价值。

M2型急性髓系白血病患者骨髓中MMSA-8、MMSA-1表达及临床意义

目的:检测急性髓系白血病患者骨髓单个核细胞中多发性骨髓瘤相关抗原(MMSA)-8、MMSA-1表达情况,探讨二者在急性髓系白血病中的临床意义。方法:纳入2019年1月-2020年1月本院M2型急性髓系白血病患者83例为研究组,同期血液科诊断为单纯缺铁性贫血患者15例为对照组,实时荧光定量PCR检测骨髓单个核细胞中MMSA-8、MMSA-1表达水平。将研究组患者根据疗效分为缓解、未缓解,根据预后状况分为预后良好、预后中等和预后不良,分析MMSA-8、MMSA-1与疗效、预后的关系。另收集研究组患者的一般资料,包括入院时血常规白细胞数、血红蛋白、血小板数和骨髓原始细胞百分比,Pearson法分析MMSA-8、MMSA-1与患者临床资料以及MMSA-8与MMSA-1的相关性。结果:对患者骨髓单个核细胞中MMSA-8、MMSA-1 mRNA水平分析结果显示,研究组患者明显高于对照组患者(P<0.05);研究组中未缓解患者明显高于缓解患者(P<0.05),预后中等、预后不良患者均明显高于预后良好患者(P<0.05),预后不良患者仅MMSA-1 mRNA水平明显高于预后中等患者(P<0.05)。Pearson法分析结果显示,研究组患者MMSA-8、MMSA-1与白细胞数呈正相关(r=0.468,r=0.516),MMSA-8与MMSA-1呈正相关(r=0.318)。结论:M2型急性髓系白血病患者骨髓单个核细胞中MMSA-8、MMSA-1水平升高,二者均与疾病发生发展关系密切,且对预后有一定预测价值。

NapA蛋白诱导巨噬细胞分泌趋化因子单核细胞趋化蛋白1和白细胞介素8的机制

目的:研究幽门螺杆菌NapA蛋白诱导巨噬细胞分泌趋化因子单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)和白细胞介素8(interleukin-8,IL-8)的机制。方法:利用NapA蛋白处理巨噬细胞,然后使用ELISA法检测上清中MCP-1和IL-8的表达量。使用C29、ST2825、SB203580、SP600125以及PDTC和巨噬细胞预先共孵育1 h,分别抑制Toll样受体2(toll-like receptors 2,TLR2)、髓样分化因子(myeloid differentiation factor 88,MyD88)、核糖核酸酶p蛋白亚基p38(ribonuclease p-protein subunit p38,p38)、应激活化蛋白激酶(c-Jun N-terminal kinase,c-Jun)和核因子κB(nuclear factor kappa B,NF-κB)的活性,然后再加入NapA蛋白孵育4 h,收集上清并检查其中MCP-1和IL-8的表达量。结果:NapA蛋白刺激巨噬细胞后,MCP-1和IL-8的表达量明显高于未处理组。利用抑制剂C29抑制TLR2的活性,NapA蛋白诱导的MCP-1和IL-8表达量降低。使用ST2825、PDTC以及SB203580分别抑制MyD88、NF-κB以及p38的活性,能够降低NapA蛋白诱导巨噬细胞分泌MCP-1和IL-8的能力,但是抑制c-Jun不影响NapA蛋白诱导巨噬细胞分泌MCP-1和IL-8。结论:幽门螺杆菌NapA蛋白通过TLR2/MyD88/NF-κB通路诱导巨噬细胞分泌MCP-1和IL-8。

血清NF-κB、TIMP-1、IL-8与儿童幽门螺杆菌感染相关性胃炎的关系

目的分析血清核因子-κB(NF-κB)、基质金属蛋白酶抑制因子-1(TIMP-1)、白细胞介素-8(IL-8)与儿童幽门螺杆菌(H.pylori)感染相关性胃炎的关系。方法选取2021年7月至2023年7月就诊的60例H.pylori感染相关性胃炎患儿设为观察组,另选取同期体检中心的60例健康体检儿童设为健康组,检测、比较2组血清NF-κB、TIMP-1、IL-8水平,比较观察组患者不同炎性活动度组血清NF-κB、TIMP-1、IL-8水平,比较观察组患者治疗前后血清NF-κB、TIMP-1、IL-8水平,绘制受试者工作曲线(ROC),分析血清NF-κB、TIMP-1、IL-8对H.pylori感染相关性胃炎的诊断效能。结果观察组血清NF-κB、TIMP-1、IL-8水平均高于健康组(P<0.05)。重度组血清NF-κB、TIMP-1、IL-8水平均高于中度组、轻度组(P<0.05),中度组血清NF-κB、TIMP-1、IL-8水平均高于轻度组(P<0.05)。治疗后血清NF-κB、TIMP-1、IL-8水平低于治疗前(P<0.05)。血清NF-κB、TIMP-1、IL-8联合检测诊断H.pylori感染相关性胃炎的AUC是0.904,95%CI可信区间是0.856~0.951,联合检测的灵敏度(94.82%)、特异度(90.13%)均高于单一检测(73.82%、71.62%)、(72.26%、68.18%)、(70.18%、64.74%),差异有统计学意义(P<0.05)。结论H.pylori感染相关性胃炎患儿血清NF-κB、TIMP-1、IL-8呈异常高表达,血清NF-κB、TIMP-1、IL-8表达量与炎性活动度及疗效存在密切联系,联合检测可提高对H.pylori感染相关性胃炎的诊断效能,值得借鉴。

血清IL-8、ESM-1、VAP-1在慢性阻塞性肺疾病临床诊断及病情评估中的应用

目的探讨慢性阻塞性肺疾病(COPD)患者血清白细胞介素-8(IL-8)、内皮特异性分子-1(ESM-1)、血管黏附蛋白-1(VAP-1)表达水平,分析其与病情程度相关性及其对COPD的诊断价值。方法选取2021年11月至2022年8月郑州大学第一附属医院收治的153例COPD患者为研究对象,其中COPD稳定期42例(设为SCOPD组)、COPD急性加重期111例(设为AECOPD组),同时选取同期于医院体检的健康志愿者51例为对照组。比较两组临床资料及血清IL-8、ESM-1、VAP-1水平。对比不同病情程度患者血清IL-8、ESM-1、VAP-1水平。分析血清各指标与肺功能、生化指标、病情程度相关性。评价血清各指标对SCOPD、AECOPD的诊断价值。结果AECOPD组、SCOPD组血清IL-8、ESM-1、VAP-1水平高于对照组,且AECOPD组高于SCOPD组(P<0.05);IL-8、ESM-1、VAP-1与生化指标、COPD评估测试表(CAT)、病情程度呈正相关,与肺功能呈负相关(P<0.05);血清各指标联合诊断SCOPD与AECOPD的曲线下面积(AUC)大于单独指标诊断(P<0.05)。结论血清IL-8、ESM-1、VAP-1与COPD病情严重程度相关,联合检测其水平对COPD具有一定诊断价值,可能作为疾病诊断、病情评估的重要指标。

lncRNA FUT8-AS1通过调控miR-142-5p/BCL2轴促进上皮性卵巢癌细胞增殖、侵袭和EMT

目的 观察FUT8-AS1基因在上皮性卵巢癌(epithelial ovarian cancer, EOC)组织和细胞株中的表达,探讨影响EOC细胞增殖、侵袭和EMT的机制。方法 采用GEPIA2和Kaplan-Meier Plotter数据库分析FUT8-AS1在EOC组织中的表达及与患者生存期的关系。收集74例EOS组织标本和60例正常组织标本,应用RT-PCR法检测FUT8-AS1、miR-142-5p、BCL2和EMT相关基因的表达;运用CCK-8和Transwell实验检测敲低FUT8-AS1基因表达对EOC细胞CAOV3增殖和侵袭的影响。利用双荧光素酶报告分析FUT8-AS1与miR-142-5p的相互作用。结果 GEPIA2和Kaplan-Meier Plotter数据库分析发现,FUT8-AS1在EOC组织中的表达显著高于正常组织(P<0.05),FUT8-AS1高表达组的总生存率显著低于FUT8-AS1低表达组(P<0.01);FUT8-AS1基因在74例EOC组织中的表达明显高于正常组织[(2.547±1.370)vs(1.330±0.831),P<0.01],与大网膜转移、淋巴结转移、FIGO分期和总生存期均相关(P<0.01或P<0.05),敲低FUT8-AS1基因可以抑制CAOV3细胞的体外增殖、侵袭能力和EMT(P<0.01或P<0.05)。双荧光素酶报告分析系统检测显示,共转染miR-142-5p mimics和野生型FUT8-AS1-WT质粒,CAOV3细胞的荧光素酶活性明显降低(P<0.05),同时转染miR-142-5p mimics可抵消CAOV3细胞中由敲低FUT8-AS1导致的BCL2基因表达下调(P<0.05)。结论 FUT8-AS1基因在EOC中呈高表达且导致预后差,敲低FUT8-AS1基因可以抑制CAOV3细胞的体外增殖、侵袭能力和EMT,FUT8-AS1基因可能通过靶向miR-142-5p/BCL2分子轴促进EOC的进展。