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“Three Beauties”Principle in the English Versions of“Jing Ye Si”--Translations by Xu and Fletcher as Examples
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作者 LUO Ya DONG Juan 《Sino-US English Teaching》 2024年第5期242-246,共5页
“Jing Ye Si”is a classical poem with international influence by the Chinese poet Li Bai in the Tang Dynasty,which expresses the poet’s deep nostalgia.This paper compares and analyzes its two English versions transl... “Jing Ye Si”is a classical poem with international influence by the Chinese poet Li Bai in the Tang Dynasty,which expresses the poet’s deep nostalgia.This paper compares and analyzes its two English versions translated by the Chinese translator Xu Yuanchong and the British translator W.J.B.Fletcher based on the“three beauties”principle(beauty in sense,beauty in sound,and beauty in form),and discovers that the English version of Xu basically achieves the unity of the“three beauties”,while the English version of Fletcher realizes the“beauty in sound and form”,but deviates from the“beauty in sense”mainly owing to the inaccurate comprehension of the moon as an image and other factors.This analysis may afford us with certain reference in poetry translation. 展开更多
关键词 “Jing Ye Si” “three beauties”principle comparative analysis TRANSLATION
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Anisotropic etching mechanisms of 4H-SiC:Experimental and first-principles insights
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作者 Guang Yang Lingbo Xu +3 位作者 Can Cui Xiaodong Pi Deren Yang Rong Wang 《Journal of Semiconductors》 EI CAS CSCD 2024年第1期42-47,共6页
Molten-alkali etching has been widely used to reveal dislocations in 4H silicon carbide(4H-SiC),which has promoted the identification and statistics of dislocation density in 4H-SiC single crystals.However,the etching... Molten-alkali etching has been widely used to reveal dislocations in 4H silicon carbide(4H-SiC),which has promoted the identification and statistics of dislocation density in 4H-SiC single crystals.However,the etching mechanism of 4H-SiC is limited misunderstood.In this letter,we reveal the anisotropic etching mechanism of the Si face and C face of 4H-SiC by combining molten-KOH etching,X-ray photoelectron spectroscopy(XPS)and first-principles investigations.The activation energies for the molten-KOH etching of the C face and Si face of 4H-SiC are calculated to be 25.09 and 35.75 kcal/mol,respectively.The molten-KOH etching rate of the C face is higher than the Si face.Combining XPS analysis and first-principles calculations,we find that the molten-KOH etching of 4H-SiC is proceeded by the cycling of the oxidation of 4H-SiC by the dissolved oxygen and the removal of oxides by molten KOH.The faster etching rate of the C face is caused by the fact that the oxides on the C face are unstable,and easier to be removed with molten alkali,rather than the C face being easier to be oxidized. 展开更多
关键词 principleS ALKALI ETCHING
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Strong invariance principle for a counterbalanced random walk
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作者 TAN Hui-qun HU Zhi-shui DONG Liang 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2024年第2期370-380,共11页
We study a counterbalanced random walkS_(n)=X_(1)+…+X_(n),which is a discrete time non-Markovian process andX_(n) are given recursively as follows.For n≥2,X_(n) is a new independent sample from some xed law̸=0 with ... We study a counterbalanced random walkS_(n)=X_(1)+…+X_(n),which is a discrete time non-Markovian process andX_(n) are given recursively as follows.For n≥2,X_(n) is a new independent sample from some xed law̸=0 with a xed probability p,andX_(n)=−X_(v(n))with probability 1−p,where v(n)is a uniform random variable on{1;…;n−1}.We apply martingale method to obtain a strong invariance principle forS_(n). 展开更多
关键词 random walk MARTINGALE invariance principle
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General Optimal Trajectory Planning:Enabling Autonomous Vehicles with the Principle of Least Action
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作者 Heye Huang Yicong Liu +4 位作者 Jinxin Liu Qisong Yang Jianqiang Wang David Abbink Arkady Zgonnikov 《Engineering》 SCIE EI CAS CSCD 2024年第2期63-76,共14页
This study presents a general optimal trajectory planning(GOTP)framework for autonomous vehicles(AVs)that can effectively avoid obstacles and guide AVs to complete driving tasks safely and efficiently.Firstly,we emplo... This study presents a general optimal trajectory planning(GOTP)framework for autonomous vehicles(AVs)that can effectively avoid obstacles and guide AVs to complete driving tasks safely and efficiently.Firstly,we employ the fifth-order Bezier curve to generate and smooth the reference path along the road centerline.Cartesian coordinates are then transformed to achieve the curvature continuity of the generated curve.Considering the road constraints and vehicle dynamics,limited polynomial candidate trajectories are generated and smoothed in a curvilinear coordinate system.Furthermore,in selecting the optimal trajectory,we develop a unified and auto-tune objective function based on the principle of least action by employing AVs to simulate drivers’behavior and summarizing their manipulation characteristics of“seeking benefits and avoiding losses.”Finally,by integrating the idea of receding-horizon optimization,the proposed framework is achieved by considering dynamic multi-performance objectives and selecting trajectories that satisfy feasibility,optimality,and adaptability.Extensive simulations and experiments are performed,and the results demonstrate the framework’s feasibility and effectiveness,which avoids both dynamic and static obstacles and applies to various scenarios with multi-source interactive traffic participants.Moreover,we prove that the proposed method can guarantee real-time planning and safety requirements compared to drivers’manipulation. 展开更多
关键词 Autonomous vehicle Trajectory planning Multi-performance objectives principle of least action
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Principles of Shiology——Revealing the Basic Rules of Human Shiance
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作者 Liu Guangwei 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第1期83-96,共14页
The objective principles of shiology are mainly reflected in three fields as food acquisition, eaters' health and shiance order. Most of the objective principles in the field of food acquisition have been revealed... The objective principles of shiology are mainly reflected in three fields as food acquisition, eaters' health and shiance order. Most of the objective principles in the field of food acquisition have been revealed by agronomy and foodstuff science. This research mainly focuses on 10 principles in the field of eaters' health and shiance order and in addition, there are also five lemmas that extend from the above principles. The 10 principles are the core theory of the shiology knowledge system, which play an important role in the objective principles revealed by human beings and constitute one of the basic principles of human civilization. Compared with the scientific principles of mathematics, physics, chemistry and economics, the principles of shiology have three characteristics as popularity, practicability and survivability. The principles of shiology in the field of eaters' health are all around us, and everyone can understand and master them. Using the principles of shiology can improve the healthy life span of 8 billion people. The principles of shiology in the field of shiance order is an important tool of social governance, which can reduce human social conflicts, reduce social involution, improve overall efficiency of social operation, and maintain the sustainable development of human beings. 展开更多
关键词 principle of shiology RULE shiance eater eating matter eatology
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Exploring the Implications of the Deformation Parameter and Minimal Length in the Generalized Uncertainty Principle
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作者 Mahgoub A. Salih Taysir M. Elmahdi 《Journal of Quantum Information Science》 CAS 2024年第1期1-14,共14页
The breakdown of the Heisenberg Uncertainty Principle occurs when energies approach the Planck scale, and the corresponding Schwarzschild radius becomes similar to the Compton wavelength. Both of these quantities are ... The breakdown of the Heisenberg Uncertainty Principle occurs when energies approach the Planck scale, and the corresponding Schwarzschild radius becomes similar to the Compton wavelength. Both of these quantities are approximately equal to the Planck length. In this context, we have introduced a model that utilizes a combination of Schwarzschild’s radius and Compton length to quantify the gravitational length of an object. This model has provided a novel perspective in generalizing the uncertainty principle. Furthermore, it has elucidated the significance of the deforming linear parameter β and its range of variation from unity to its maximum value. 展开更多
关键词 Generalized Uncertainty principle Deformed Heisenberg Algebra Minimal Length
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An 8-Node Plane Hybrid Element for StructuralMechanics Problems Based on the Hellinger-Reissner Variational Principle
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作者 Haonan Li WeiWang +1 位作者 Quan Shen Linquan Yao 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第2期1277-1299,共23页
The finite element method (FEM) plays a valuable role in computer modeling and is beneficial to the mechanicaldesign of various structural parts. However, the elements produced by conventional FEM are easily inaccurat... The finite element method (FEM) plays a valuable role in computer modeling and is beneficial to the mechanicaldesign of various structural parts. However, the elements produced by conventional FEM are easily inaccurate andunstable when applied. Therefore, developing new elements within the framework of the generalized variationalprinciple is of great significance. In this paper, an 8-node plane hybrid finite element with 15 parameters (PHQ8-15β) is developed for structural mechanics problems based on the Hellinger-Reissner variational principle.According to the design principle of Pian, 15 unknown parameters are adopted in the selection of stress modes toavoid the zero energy modes.Meanwhile, the stress functions within each element satisfy both the equilibrium andthe compatibility relations of plane stress problems. Subsequently, numerical examples are presented to illustrate theeffectiveness and robustness of the proposed finite element. Numerical results show that various common lockingbehaviors of plane elements can be overcome. The PH-Q8-15β element has excellent performance in all benchmarkproblems, especially for structures with varying cross sections. Furthermore, in bending problems, the reasonablemesh shape of the new element for curved edge structures is analyzed in detail, which can be a useful means toimprove numerical accuracy. 展开更多
关键词 8-node plane hybrid element Hellinger-Reissner variational principle locking behaviors structural mechanics problems
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International law obligations for the disposal of Fukushima nuclear-contaminated water under the principles of nuclear safety
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作者 Wei Gong 《Chinese Journal of Population,Resources and Environment》 2024年第1期10-19,共10页
The disposal of contaminated water from Japan’s Fukushima nuclear power plant is a significant international nuclear safety issue with considerable cross-border implications.This matter requires compliance not only w... The disposal of contaminated water from Japan’s Fukushima nuclear power plant is a significant international nuclear safety issue with considerable cross-border implications.This matter requires compliance not only with the law of the sea but also with the principles of nuclear safety under international law.These principles serve as the overarching tenet of international and China’s domestic nuclear laws,applicable to nuclear facilities and activities.The principle of safety in nuclear activities is fully recognized in international and domestic laws,carrying broad legal binding force.Japan’s discharge of nuclear-contaminated water into the sea violates its obligations under the principle of safety in nuclear activities,including commitments to optimum protection,as low as reasonably practicable,and prevention.The Japanese government and the International Atomic Energy Agency(IAEA)have breached the obligation of optimum protection by restricting the scope of assessments,substituting core concepts,and shielding dissenting views.In the absence of clear radiation standards,they have acted unilaterally without fulfilling the obligation as low as reasonably practicable principle.The discharge of Fukushima nuclear-contaminated water poses an imminent and unpredictable risk to all countries worldwide,including Japanese residents.Japan and the IAEA should fulfill their obligations under international law regarding disposal,adhering to the principles of nuclear safety,including optimum protection,the obligation as low as reasonably practicable,and prevention through multilateral cooperation.Specifically,the obligation to provide optimum protection should be implemented by re-evaluating the most reliable disposal technologies and methods currently available and comprehensively assessing various options.The standard of the obligation as low as reasonably practicable requires that the minimization of negative impacts on human health,livelihoods,and the environment should not be subordinated to considerations of cutting costs and expenses.Multilateral cooperation should be promoted through the establishment of sound multilateral long-term monitoring mechanisms for the discharge of nuclear-contaminated water,notification and consultation obligations,and periodic assessments.These obligations under international law were fulfilled after the accidents at the Three Mile Island and Chernobyl nuclear power plants.The implications of the principles of nuclear safety align with the concept of building a community of shared future for nuclear safety advocated by China.In cases of violations of international law regarding the disposal of nuclear-contaminated water that jeopardize the concept of a community of a shared future for nuclear safety,China can also rely on its own strength to promote the implementation of due obligations through self-help. 展开更多
关键词 principles of nuclear safety Disposal of nuclear-contaminated water Optimum protection Prevention As low as reasonably achievable
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Adaptive Optimal Discrete-Time Output-Feedback Using an Internal Model Principle and Adaptive Dynamic Programming
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作者 Zhongyang Wang Youqing Wang Zdzisław Kowalczuk 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第1期131-140,共10页
In order to address the output feedback issue for linear discrete-time systems, this work suggests a brand-new adaptive dynamic programming(ADP) technique based on the internal model principle(IMP). The proposed metho... In order to address the output feedback issue for linear discrete-time systems, this work suggests a brand-new adaptive dynamic programming(ADP) technique based on the internal model principle(IMP). The proposed method, termed as IMP-ADP, does not require complete state feedback-merely the measurement of input and output data. More specifically, based on the IMP, the output control problem can first be converted into a stabilization problem. We then design an observer to reproduce the full state of the system by measuring the inputs and outputs. Moreover, this technique includes both a policy iteration algorithm and a value iteration algorithm to determine the optimal feedback gain without using a dynamic system model. It is important that with this concept one does not need to solve the regulator equation. Finally, this control method was tested on an inverter system of grid-connected LCLs to demonstrate that the proposed method provides the desired performance in terms of both tracking and disturbance rejection. 展开更多
关键词 Adaptive dynamic programming(ADP) internal model principle(IMP) output feedback problem policy iteration(PI) value iteration(VI)
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Four Dimensions of Integrating Chinese Culture Into College English Teaching From the Perspective of Tyler’s Basic Principles
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作者 CHEN Lijuan 《Sino-US English Teaching》 2024年第5期232-237,共6页
Integrating Chinese culture into college English can not only enhance students’humanities literacy and cultivate their cultural confidence,but also facilitate the inheritance and international dissemination of Chines... Integrating Chinese culture into college English can not only enhance students’humanities literacy and cultivate their cultural confidence,but also facilitate the inheritance and international dissemination of Chinese culture.Taking Tyler’s curriculum framework as the starting point,this paper analyzes some factors that affect the integration of Chinese culture into the college English teaching and proposes some strategies for the integration of Chinese culture into college English teaching by innovating teaching objectives,enriching teaching contents,transforming modes of course delivery,and reconstructing the assessment system. 展开更多
关键词 Tyler’s basic principles Chinese culture college English
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Critical Analysis of the Origins of Heisenberg's Uncertainty Principle
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作者 André Michaud 《Journal of Modern Physics》 2024年第6期765-795,共31页
Analysis of the initial stages of the logical process followed by Louis de Broglie in establishing the electron phase wave equation in his 1924 thesis, which triggered the development of Wave Mechanics when Erwin Schr... Analysis of the initial stages of the logical process followed by Louis de Broglie in establishing the electron phase wave equation in his 1924 thesis, which triggered the development of Wave Mechanics when Erwin Schrödinger formalized this concept with his vectorial wave equation. This development was soon followed by Quantum Mechanics, when Schrödinger proved that the Matrix Mechanics independently developed by Werner Heisenberg was equivalent to Wave Mechanics, with both theories leaving room for some degree of uncertainty as to the physical localization of the moving electron. This is what led Heisenberg to also formalize the Uncertainty Principle to take this situation into account. This principle was soon regarded as a fundamental axiomatic principle that seemed to make further exploration of the subatomic level of magnitude appear impossible to most researchers. We will analyze in this article the reason why the phase-wave velocity established by de Broglie generated this uncertainty in the localization of the moving electron in light of the current state of knowledge on the behavior of the electron in motion, in view of establishing the relevance of maintaining the Uncertainty Principle in the study of the subatomic level of magnitude. 展开更多
关键词 Phase Wave Velocity Wave Mechanics Matrix Mechanics Quantum Mechanics Uncertainty principle
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The Accelerated Expansion of the Universe in the Light of the Maximum Ordinality Principle
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作者 Corrado Giannantoni 《Journal of Applied Mathematics and Physics》 2024年第2期585-602,共18页
The main aim of the paper is to present (and at the same time offer) a differ-ent perspective for the analysis of the accelerated expansion of the Universe. A perspective that can surely be considered as being “in pa... The main aim of the paper is to present (and at the same time offer) a differ-ent perspective for the analysis of the accelerated expansion of the Universe. A perspective that can surely be considered as being “in parallel” to the tradition-al ones, such as those based, for example, on the hypotheses of “Dark Matter” and “Dark Energy”, or better as a “com-possible” perspective, because it is not understood as being “exclusive”. In fact, it is an approach that, when con-firmed by experimental results, always keeps its validity from an “operative” point of view. This is because, in analogy to the traditional perspectives, on the basis of Popper’s Falsification Principle the corresponding “Generative” Logic on which it is based has not the property of the perfect induction. The basic difference then only consists in the fact that the Evolution of the Universe is now modeled by considering the Universe as a Self-Organizing System, which is thus analyzed in the light of the Maximum Ordinality Principle. 展开更多
关键词 Accelerated Expansion of the Universe Maximum Ordinality principle Incip-ient Differential Calculus
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A Solution to the Cosmological Constant Problem Using the Holographic Principle (A Brief Note)
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作者 Eugene Terry Tatum 《Journal of Modern Physics》 2024年第2期159-166,共8页
This paper integrates a quantum conception of the Planck epoch early universe with FSC model formulae and the holographic principle, to offer a reasonable explanation and solution of the cosmological constant problem.... This paper integrates a quantum conception of the Planck epoch early universe with FSC model formulae and the holographic principle, to offer a reasonable explanation and solution of the cosmological constant problem. Such a solution does not appear to be achievable in cosmological models which do not integrate black hole formulae with quantum formulae such as the Stephan-Boltzmann law. As demonstrated herein, assuming a constant value of Lambda over the great span of cosmic time appears to have been a mistake. It appears that Einstein’s assumption of a constant, in terms of vacuum energy density, was not only a mistake for a statically-balanced universe, but also a mistake for a dynamically-expanding universe. 展开更多
关键词 Quantum Cosmology Planck Scale Cosmological Constant Black Holes Holographic principle Flat Space Cosmology AdS-CFT ER = EPR Cosmology Model
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Research on Teaching Methods of Flight Control Principles
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作者 Dongying Li 《Journal of Electronic Research and Application》 2024年第3期155-160,共6页
With the rapid development of China’s civil aviation industry,the teaching method of operating knowledge of flight principles has changed greatly,which creates a good implementation environment to improve the safety ... With the rapid development of China’s civil aviation industry,the teaching method of operating knowledge of flight principles has changed greatly,which creates a good implementation environment to improve the safety of civil aviation in our country.At present,the main training content of air route transport pilots in China is basic aviation theory,initial flight training,airline modification,etc.The principles of flight control are an important part of basic aviation theoretical knowledge training,which will involve a large number of flight technology training content,instructors will also be based on the pilot type.Teaching flight control theory and practical knowledge requires relatively high theoretical learning ability of students,and the learning effect of this part of theoretical knowledge will directly affect the quality of subsequent learning,but also directly affect the effectiveness of flight training.This paper focuses on the analysis of the basic concepts of flight control,studies the existing problems in the teaching of flight control principles,summarizes the teaching measures of flight control principles,aiming to provide a reference to teaching personnel. 展开更多
关键词 Flight control principle knowledge Virtual simulation Teaching method
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Research on the Course of Principles of Concrete Structure Design
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作者 Yuhuan Shi Jinping Hu +2 位作者 Xue Lin Zhinan Jiang Jialian Qi 《Journal of Contemporary Educational Research》 2024年第2期80-84,共5页
Nowadays,education and teaching have become a hot topic,and teaching in colleges and universities is facing a brand-new development direction.Principles of Concrete Structure Design,as one of the main courses,transmit... Nowadays,education and teaching have become a hot topic,and teaching in colleges and universities is facing a brand-new development direction.Principles of Concrete Structure Design,as one of the main courses,transmits professional knowledge for students,enhances the students’professional ability,and further carries out in-depth research on the course to bring a better teaching effect for students.The article mainly focuses on the research of the principles of concrete structure design course,conducts an analysis of the teaching characteristics of the principles of concrete structure design course,and reasonably sets the teaching content from the optimization of the course teaching objectives;innovative course teaching methods can deepen the effect of knowledge understanding;reform of experimental practice teaching can lay down the effect of the internalization of knowledge,etc.The in-depth description and discussion of the relevant aspects of the research aim to provide guidelines for related research. 展开更多
关键词 Concrete structure design principle Course characteristics Teaching innovation Course research
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First-Principles Investigation of Charge Transfer Mechanism of B-Doped 3C-SiC Semiconductor Material
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作者 Abdullahi Alkali Dauda Muhammad Yusuf Onimisi +7 位作者 Adeyemi Joshua Owolabi Hameed Adeneyi Lawal Hassan Muhammad Gambo Bashir Mohammed Aliyu Surajo Bala Muhammad Lamido Madugu Muhammad Abdurrahman Nainna Johnson Akinade Bamikole 《World Journal of Condensed Matter Physics》 CAS 2024年第2期35-44,共10页
This study delves into the charge transfer mechanism of boron (B)-doped 3C-SiC through first-principles investigations. We explore the effects of B doping on the electronic properties of 3C-SiC, focusing on a 12.5% im... This study delves into the charge transfer mechanism of boron (B)-doped 3C-SiC through first-principles investigations. We explore the effects of B doping on the electronic properties of 3C-SiC, focusing on a 12.5% impurity concentration. Our comprehensive analysis encompasses structural properties, electronic band structures, and charge density distributions. The optimized lattice constant and band gap energy of 3C-SiC were found to be 4.373 Å and 1.36 eV respectively, which is in agreement with previous research (Bui, 2012;Muchiri et al., 2018). Our results show that B doping narrows the band gap, enhances electrical conductivity, and influences charge transfer interactions. The charge density analysis reveals substantial interactions between B dopants and surrounding carbon atoms. This work not only enhances our understanding of the material’s electronic properties, but also highlights the importance of charge density analysis for characterizing charge transfer mechanisms and their implications in the 3C-SiC semiconductors. 展开更多
关键词 First-principles Calculations DFT Boron (B)-Doped 3C-SiC Charge Transfer
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人参皂苷Rc通过调控AMPK通路介导的细胞焦亡减轻小鼠脑缺血再灌注损伤
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作者 李良勇 马莉 +1 位作者 李亚军 梅灯 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第5期844-851,共8页
目的:探讨人参皂苷Rc对脑缺血再灌注损伤(CIRI)小鼠的保护作用,并从细胞焦亡角度阐释其作用机制。方法:将C57BL/6小鼠随机分为假手术(sham)组、大脑中动脉闭塞/再灌注(MCAO/R)组、人参皂苷Rc+MCAO/R组(Rc组)、AMP活化蛋白激酶(AMPK)激... 目的:探讨人参皂苷Rc对脑缺血再灌注损伤(CIRI)小鼠的保护作用,并从细胞焦亡角度阐释其作用机制。方法:将C57BL/6小鼠随机分为假手术(sham)组、大脑中动脉闭塞/再灌注(MCAO/R)组、人参皂苷Rc+MCAO/R组(Rc组)、AMP活化蛋白激酶(AMPK)激动剂阿卡地新(AICAR)+MCAO/R组(agonist组)和人参皂苷Rc+MCAO/R+AMPK抑制剂Compound C组(Rc+inhibitor组)。对agonist组小鼠给予AICAR(500 mg/kg)腹腔注射,Rc+inhibitor组小鼠给予Compound C(20 mg/kg)腹腔注射;Rc组和Rc+inhibitor组小鼠造模后给予人参皂苷Rc(40 mg/kg)灌胃,每天一次,连续7 d;sham组和MCAO/R组则给予等体积的纯化水。Zea Longa评分观察小鼠神经功能缺损程度,TTC染色观察小鼠脑梗死体积,干湿重法观察小鼠脑水肿程度,HE染色观察小鼠脑组织病理形态的改变,RT-qPCR和Western blot检测小鼠脑组织AMPK、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、胱天蛋白酶1(caspase-1)和焦亡效应蛋白消皮素D(GSDMD)的表达,ELISA检测炎症因子白细胞介素1β(IL-1β)和IL-18的含量。结果:Rc组和agonist组小鼠神经功能缺损症状减轻,脑梗死体积减小,脑水肿和神经元病理损伤受到抑制,脑组织pAMPK/AMPK比值和AMPK mRNA表达水平升高,细胞焦亡相关蛋白NLRP3、caspase-1和GSDMD的mRNA和蛋白表达水平降低,炎症因子IL-1β和IL-18表达水平降低。Rc+inhibitor组小鼠脑组织p-AMPK/AMPK比值和AMPK mRNA表达水平较Rc组显著下降(P<0.05),NLRP3、caspase-1和GSDMD的mRNA和蛋白表达水平,以及IL-1β和IL-18表达水平显著升高(P<0.05),小鼠神经功能缺损程度评分升高,脑梗死体积增大,脑水肿和神经元病理损伤显著加重(P<0.05)。结论:人参皂苷Rc可能通过活化AMPK通路抑制NLRP3/caspase-1/GSDMD介导的细胞焦亡,从而减轻小鼠CIRI。 展开更多
关键词 人参皂苷Rc 脑缺血再灌注损伤 amp活化蛋白激酶 细胞焦亡
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基于NOD2介导的AMPK/mTOR信号通路探讨宫颈癌细胞恶性行为的机制
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作者 杜瑞亭 伍东月 +1 位作者 郭清民 靳冬梅 《安徽医科大学学报》 CAS 北大核心 2024年第2期316-324,共9页
目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC... 目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC)以及NOD2过表达(NOD2)质粒和载体(Vec)转染CC细胞。通过CCK-8测定、集落形成和Transwell细胞侵袭测定来确定NOD2对CC细胞生长的影响。通过高通量RNA测序(RNA-Seq)进行转录组分析。Western blot试验检测细胞系中NOD2、AMPK/mTOR信号通路和自噬蛋白的表达。24只雌性BALB/c裸鼠随机分为4组,每组6只:载体组(Vec组)、NOD2过表达组(NOD2组)、shNC组和shNOD2组。构建小鼠远处转移模型,监测肺转移的荧光强度,计数肺转移结节的数量。结果 在线数据库分析显示,NOD2在CC组织中表达明显高于正常组织,并且不同分期的CC中NOD2的mRNA表达差异有统计学意义(P<0.05)。此外,NOD2的高表达与较差的总生存期和无病生存期相关(P<0.05)。NOD2过表达对CC细胞增殖、集落形成、迁移和侵袭具有促进作用,而NOD2敲低则相反。与体外结果一致,在转移的小鼠尾静脉注射模型中,NOD2组CC细胞的肺定殖、肺转移灶较Vec组增加(P<0.05),而shNOD2组CC细胞的肺定殖、肺转移灶较shNC组减少(P<0.05)。RNA-Seq结果显示NOD2表达与AMPK信号激活、mTOR信号抑制、自噬调节途径激活和自噬体形成显著相关。与shNC组相比,shNOD2组磷酸化AMPK、LC3蛋白表达水平减少(P<0.05),磷酸化mTOR、p62蛋白表达水平增加(P<0.05);与Vec组相比,NOD2组LC3、AMPK蛋白表达水平增加(P<0.05),磷酸化mTOR、p62蛋白表达水平减少(P<0.05)。与shNC组相比,shNOD2组GFP-mRFP-LC3的点积累减少(P<0.05);与Vec组相比,GFP-mRFP-LC3的点积累增加(P<0.05)。结论 NOD2可能通过AMPK/mTOR信号促进CC增殖、迁移和侵袭,其作用机制部分涉及自噬激活。 展开更多
关键词 核苷酸结合寡聚化结构域受体2 amp活化蛋白激酶 雷帕霉素靶蛋白 宫颈癌细胞 自噬
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黄芩苷调节cAMP/PKA/CREB信号通路对湿疹大鼠皮肤屏障功能的影响 被引量:1
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作者 王首帆 徐宜厚 +1 位作者 徐爱琴 朱立宏 《天津医药》 CAS 2024年第2期148-153,共6页
目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、... 目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、高剂量BA组(BA-H组,100 mg/kg)、泼尼松组(PNS组,25 mg/kg)、BAH+cAMP抑制剂(SQ22536)组(100 mg/kg+2.13 mg/kg)、BA-H+PKA抑制剂(H-89)组(100 mg/kg+5 mg/kg),每组12只。除NC组外,其余组大鼠均构建湿疹大鼠模型。建模成功2 d后,分组进行给药处理。检测湿疹面积及严重度指数(EASI)评分、经皮肤水分流失量(TEWL)、角质层含水量(WCSC)变化;酶联免疫吸附试验(ELISA)检测大鼠血清中免疫球蛋白E(IgE)、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平及大鼠背部受试区皮损组织中c AMP蛋白表达;HE染色检测大鼠背部受试区皮损组织病理变化;Western blot检测大鼠背部受试区皮损组织中水通道蛋白3(AQP3)、cathelicidin相关抗菌肽(CRAMP)、p-PKA、p-CREB蛋白表达。结果与NC组比较,Model组大鼠背部受试区皮损组织病理损伤严重,EASI评分、TEWL、IgE、IL-4水平升高,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平降低(P<0.05)。与Model组比较,BA-L组、BA-M组、BA-H组、PNS组大鼠背部受试区皮损组织病理损伤减轻,EASI评分、TEWL、IgE、IL-4水平降低,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平升高(P<0.05);且BA-L组、BA-M组、BA-H组上述指标变化呈剂量依赖性。SQ22536或H-89减弱了高剂量BA对湿疹大鼠皮肤屏障功能的改善作用。结论BA可能通过激活cAMP/PKA/CREB信号通路改善湿疹大鼠皮肤屏障功能。 展开更多
关键词 湿疹 黄芩苷 环磷酸腺苷/蛋白激酶A/camp反应元件结合蛋白通路 皮肤屏障功能
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基于AMPK通路探讨茶多酚改善糖尿病大鼠糖脂代谢机制
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作者 黄苏萍 林欢 +2 位作者 曾淑华 杨柳媛 许陆达 《山东中医药大学学报》 2024年第2期192-200,共9页
目的:探讨茶多酚从AMP活化蛋白激酶(AMPK)通路途径调控糖尿病大鼠糖脂代谢的机制。方法:经糖耐量试验筛选6周龄SD大鼠45只,按随机数字表法分为正常组10只和造模组35只,造模组糖尿病造模成功后剩余30只,根据体质量分层法和血糖水平分为... 目的:探讨茶多酚从AMP活化蛋白激酶(AMPK)通路途径调控糖尿病大鼠糖脂代谢的机制。方法:经糖耐量试验筛选6周龄SD大鼠45只,按随机数字表法分为正常组10只和造模组35只,造模组糖尿病造模成功后剩余30只,根据体质量分层法和血糖水平分为模型组、二甲双胍组和茶多酚组,每组各10只。各组均于每日下午灌胃,正常组与模型组给予无菌生理盐水,二甲双胍组给予二甲双胍水溶液,茶多酚组给予茶多酚。干预120 d后,检测各组大鼠的空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C);酶联免疫吸附试验法检测肝脏乙酰辅酶A羧化酶(ACC)和激素敏感性脂肪酶(HSL);实时荧光定量法检测肝脏ACCmRNA、转录因子胆固醇调节元件结合蛋白1(SREBP-1)mRNA、3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA;免疫印迹法检测肝脏AMPKα1、磷酸化AMP活化蛋白激酶α1(P-AMPKα1)、SREBP1、HMGCR、肉毒碱棕榈酰转移酶1(CPT1)、葡萄糖转运蛋白4(GLUT4)和磷酸烯醇丙酮酸羧化激酶(PEPCK)。结果:与正常组比较,模型组大鼠的一般情况较差,FBG、TG、TC、LDL-C、ACC升高(P<0.05),SREBP1、HMGCR蛋白及mRNA表达升高(P<0.05),CPTI、PEPCK蛋白表达升高(P<0.05),HDL-C、HSL降低(P<0.05),AMPKα1、P-AMPKα1、GLUT4蛋白表达降低(P<0.05)。与模型组比较,茶多酚组大鼠的一般情况改善,TG、LDL-C、ACC降低(P<0.05),SREBP1、HMGCR蛋白及mRNA表达降低(P<0.05),PEPCK蛋白表达降低(P<0.05),HDL-C、HSL升高(P<0.05),AMPKα1、P-AMPKα1、GLUT4、CPT1蛋白表达升高(P<0.05)。与二甲双胍组比较,茶多酚组TC升高(P<0.05),FBG、TG、LDL-C、HDL-C、HSL、ACC、ACC mRNA、HMGCR mRNA、SREBP1 mRNA、AMPKα1、P-AMPKα1、SREBP1、HMGCR、CPT1、GLUT4、PEPCK相对表达量差异无统计学意义(P>0.05)。结论:茶多酚可能通过调节AMPK通路,影响HSL、HMGCR、SREBP1、CPTI、GLUT4和PEPCK,从而改善糖尿病大鼠糖脂代谢。 展开更多
关键词 糖尿病 茶多酚 二甲双胍 amp活化蛋白激酶通路 糖脂代谢 大鼠
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