Mechanisms regulating neurogenesis involve broad and complex processes that represent intriguing therapeutic targets in the field of regenerative medicine.One influential factor guiding neural stem cell proliferation ...Mechanisms regulating neurogenesis involve broad and complex processes that represent intriguing therapeutic targets in the field of regenerative medicine.One influential factor guiding neural stem cell proliferation and cellular differentiation during neurogenesis are epigenetic mechanisms.We present an overview of epigenetic mechanisms including chromatin structure and histone modifications;and discuss novel roles of two histone modifiers,Ezh2 and Suv4-20h1/Suv4-20h2(collectively referred to as Suv4-20h),in neurodevelopment and neurogenesis.This review will focus on broadly reviewing epigenetic regulatory components,the roles of epigenetic components during neurogenesis,and potential applications in regenerative medicine.展开更多
AIM: To evaluate the serologic IgG response to H pylori and CagA across age groups and in healthy children and adults. METHODS: Totally, 386 children aged 1-15 years and 200 adults aged 20-60 years, were enrolled to s...AIM: To evaluate the serologic IgG response to H pylori and CagA across age groups and in healthy children and adults. METHODS: Totally, 386 children aged 1-15 years and 200 adults aged 20-60 years, were enrolled to study. The serum samples of participant were tested for presence of anti-H pylori and anti-CagA IgG by using ELISA method. RESULTS: The seroprevalence of H pylori in adults was signif icantly higher than that observed in children (67.5/ vs 46.6/; P < 0.000003). In children, the seropositivity rate in males (51.9/) was signifi cantly (P < 0.05) higher than that observed in females (41.7/). The prevalence of serum anti-CagA antibody was 72.8/ and 67.4/ in infected children and adults, respectively. The mean titer of serum anti-CagA antibodies was significantly higher among children in comparison to adults (64.1 Uarb/mL vs 30.7; P < 0.03). In infected children and adults the prevalence of serum anti-CagA antibody was higher in males compared to females (78.4/ vs 66.3/; P = 0.07 and 75.6/ vs 54.71/; P < 0.04, respectively). The age-specific prevalence of anti-H pylori and anti-CagA antibody (in infected subjects) was 37.6/ and 59.57/ at age 1-5 years, 46.9/ and 75/ at age 6-10 years, 54.9/ and 79.45/ at age 11-15, 59.01/ and 83.33/ at age 20-30 years, 66.6/ and 60.52/ at age 31-40 years, 73.46/ and 63.88/ at age 41-50 years and 75.75/ and 60/ at age 51-60 years with mean titer of anti-CagA antibody of 75.94, 63.32, 57.11, 52.06, 23.62, 21.52 and 21.80 Uarb/mL, respectively. There was significant difference between mean serum anti-CagA antibody in age subgroups (P < 0.001). CONCLUSION: These results showed that anti-H pylori and anti-CagA antibodies were common in the children and adults. The H pylori-specifi c antibodies influenced by age and sex of subjects. Moreover, it seems that malesare more susceptible to infection with CagA+ strains compared to females. The seroprevalence of anti-CagA antibody was increased with age, up to 30 years and then decreased. It was also found that the magnitude of the IgG response to CagA decreased with advanced age.展开更多
目的：构建人羧基肽酶 H 不同区段抗原的原核表达载体，诱导表达获得重组蛋白，初步验证人羧基肽酶 H 不同抗原区段在羧基肽酶 H 自身抗体检测中的应用价值。方法应用 RT-PCR 方法调取目的基因，构建相应的原核表达质粒，转化大肠埃希...目的：构建人羧基肽酶 H 不同区段抗原的原核表达载体，诱导表达获得重组蛋白，初步验证人羧基肽酶 H 不同抗原区段在羧基肽酶 H 自身抗体检测中的应用价值。方法应用 RT-PCR 方法调取目的基因，构建相应的原核表达质粒，转化大肠埃希氏菌诱导表达重组蛋白，用重组蛋白作为包被抗原建立人羧基肽酶 H 自身抗体的间接 ELISA 方法，评价羧基肽酶 H 抗原在检测新诊断2型糖尿病患者抗羧基肽酶 H 自身抗体中的应用价值。结果获得了羧基肽酶 H 三种不同区段抗原，其中42～476氨基酸区段为较理想的抗原。以该全长抗原作为包被抗原检测95例新诊断2型糖尿病患者，羧基肽酶 H 自身抗体阳性率为8.42％。结论原核克隆表达的人羧基肽酶 H 42～476氨基酸区段抗原具有良好的抗原性，可作为成人隐匿性自身免疫性糖尿病鉴别诊断的候选抗原。展开更多
E-learning platforms support education systems worldwide, transferring theoretical knowledge as well as soft skills. In the present study high-school pupils’, and adult students’ opinions were evaluated through a mo...E-learning platforms support education systems worldwide, transferring theoretical knowledge as well as soft skills. In the present study high-school pupils’, and adult students’ opinions were evaluated through a modern structured MOODLE interactive course, designed for the needs of the laboratory course “Automotive Systems”. The study concerns Greek secondary vocational education pupils aged 18 and vocational training adult students aged 20 to 50 years. The multistage, equal size simple random cluster sample was used as a sampling method. Pupils and adult students of each cluster completed structured 10-question questionnaires both before and after attending the course. A total of 120 questionnaires were collected. In general, our findings disclosed that the majority of pupils and adult students had significantly improved their knowledge and skills from using MOODLE. They reported strengthening conventional teaching, using the new MOODLE technology. The satisfaction indices improved quite, with the differences in their mean values being statistically significant.展开更多
目的了解天津市成人尿钠水平与高血压患病风险的关联,为高血压及相关疾病的防治提供依据。方法本研究数据来自天津市2018年成人慢性病与营养监测,采用多阶段整群随机抽样方法,抽取4423名≥18岁的常住居民进行相关研究。通过问卷调查收...目的了解天津市成人尿钠水平与高血压患病风险的关联,为高血压及相关疾病的防治提供依据。方法本研究数据来自天津市2018年成人慢性病与营养监测,采用多阶段整群随机抽样方法,抽取4423名≥18岁的常住居民进行相关研究。通过问卷调查收集调查对象的人口学特征,通过体格测量收集调查对象的高血压患病等情况,并以晨尿检测结果为点尿钠水平估算24 h尿钠水平。采用SPSS 24.0进行t检验、方差分析、多重线性回归和多因素logistic回归分析。结果加权后,研究对象的高血压患病率为39.8%,单纯收缩期高血压患病率为19.7%,单纯舒张期高血压患病率为3.3%;收缩压中位数为134.00 mm Hg(P_(25)~P_(75):121.00~148.00 mm Hg),舒张压中位数为80.33 mm Hg(P_(25)~P_(75):72.33~88.33 mm Hg);24 h尿钠水平中位数为211.45 mmol/d(P_(25)~P_(75):173.13~254.36 mmol/d)。高血压、单纯收缩期高血压、单纯舒张期高血压患者24 h尿钠水平均高于相应的非患病人群,差异均有统计学意义(P<0.01)。多重线性回归分析调整相关因素后结果显示,24 h尿钠与收缩压(β=0.17)和舒张压(β=0.13)均呈正相关。多因素logistic回归分析结果显示,调整性别、年龄、教育程度、饮酒、吸烟、高血压家族史、BMI和糖尿病后,与24 h尿钠水平最低四分位数组相比,尿钠水平最高四分位数组患高血压、单纯收缩期高血压的风险较高,OR值分别为2.15(95%CI:1.74~2.65)、1.61(95%CI:1.25~2.08),均有统计学意义(P<0.01),单纯舒张期高血压的患病风险无统计学意义(OR=1.23,95%CI:0.73~2.08,P>0.05)。结论24 h尿钠水平与高血压、单纯收缩期高血压存在关联,应开展有效的干预和广泛的科普宣传,降低广大群众的食盐摄入量。展开更多
文摘Mechanisms regulating neurogenesis involve broad and complex processes that represent intriguing therapeutic targets in the field of regenerative medicine.One influential factor guiding neural stem cell proliferation and cellular differentiation during neurogenesis are epigenetic mechanisms.We present an overview of epigenetic mechanisms including chromatin structure and histone modifications;and discuss novel roles of two histone modifiers,Ezh2 and Suv4-20h1/Suv4-20h2(collectively referred to as Suv4-20h),in neurodevelopment and neurogenesis.This review will focus on broadly reviewing epigenetic regulatory components,the roles of epigenetic components during neurogenesis,and potential applications in regenerative medicine.
文摘AIM: To evaluate the serologic IgG response to H pylori and CagA across age groups and in healthy children and adults. METHODS: Totally, 386 children aged 1-15 years and 200 adults aged 20-60 years, were enrolled to study. The serum samples of participant were tested for presence of anti-H pylori and anti-CagA IgG by using ELISA method. RESULTS: The seroprevalence of H pylori in adults was signif icantly higher than that observed in children (67.5/ vs 46.6/; P < 0.000003). In children, the seropositivity rate in males (51.9/) was signifi cantly (P < 0.05) higher than that observed in females (41.7/). The prevalence of serum anti-CagA antibody was 72.8/ and 67.4/ in infected children and adults, respectively. The mean titer of serum anti-CagA antibodies was significantly higher among children in comparison to adults (64.1 Uarb/mL vs 30.7; P < 0.03). In infected children and adults the prevalence of serum anti-CagA antibody was higher in males compared to females (78.4/ vs 66.3/; P = 0.07 and 75.6/ vs 54.71/; P < 0.04, respectively). The age-specific prevalence of anti-H pylori and anti-CagA antibody (in infected subjects) was 37.6/ and 59.57/ at age 1-5 years, 46.9/ and 75/ at age 6-10 years, 54.9/ and 79.45/ at age 11-15, 59.01/ and 83.33/ at age 20-30 years, 66.6/ and 60.52/ at age 31-40 years, 73.46/ and 63.88/ at age 41-50 years and 75.75/ and 60/ at age 51-60 years with mean titer of anti-CagA antibody of 75.94, 63.32, 57.11, 52.06, 23.62, 21.52 and 21.80 Uarb/mL, respectively. There was significant difference between mean serum anti-CagA antibody in age subgroups (P < 0.001). CONCLUSION: These results showed that anti-H pylori and anti-CagA antibodies were common in the children and adults. The H pylori-specifi c antibodies influenced by age and sex of subjects. Moreover, it seems that malesare more susceptible to infection with CagA+ strains compared to females. The seroprevalence of anti-CagA antibody was increased with age, up to 30 years and then decreased. It was also found that the magnitude of the IgG response to CagA decreased with advanced age.
文摘目的：构建人羧基肽酶 H 不同区段抗原的原核表达载体，诱导表达获得重组蛋白，初步验证人羧基肽酶 H 不同抗原区段在羧基肽酶 H 自身抗体检测中的应用价值。方法应用 RT-PCR 方法调取目的基因，构建相应的原核表达质粒，转化大肠埃希氏菌诱导表达重组蛋白，用重组蛋白作为包被抗原建立人羧基肽酶 H 自身抗体的间接 ELISA 方法，评价羧基肽酶 H 抗原在检测新诊断2型糖尿病患者抗羧基肽酶 H 自身抗体中的应用价值。结果获得了羧基肽酶 H 三种不同区段抗原，其中42～476氨基酸区段为较理想的抗原。以该全长抗原作为包被抗原检测95例新诊断2型糖尿病患者，羧基肽酶 H 自身抗体阳性率为8.42％。结论原核克隆表达的人羧基肽酶 H 42～476氨基酸区段抗原具有良好的抗原性，可作为成人隐匿性自身免疫性糖尿病鉴别诊断的候选抗原。
文摘E-learning platforms support education systems worldwide, transferring theoretical knowledge as well as soft skills. In the present study high-school pupils’, and adult students’ opinions were evaluated through a modern structured MOODLE interactive course, designed for the needs of the laboratory course “Automotive Systems”. The study concerns Greek secondary vocational education pupils aged 18 and vocational training adult students aged 20 to 50 years. The multistage, equal size simple random cluster sample was used as a sampling method. Pupils and adult students of each cluster completed structured 10-question questionnaires both before and after attending the course. A total of 120 questionnaires were collected. In general, our findings disclosed that the majority of pupils and adult students had significantly improved their knowledge and skills from using MOODLE. They reported strengthening conventional teaching, using the new MOODLE technology. The satisfaction indices improved quite, with the differences in their mean values being statistically significant.
文摘目的了解天津市成人尿钠水平与高血压患病风险的关联,为高血压及相关疾病的防治提供依据。方法本研究数据来自天津市2018年成人慢性病与营养监测,采用多阶段整群随机抽样方法,抽取4423名≥18岁的常住居民进行相关研究。通过问卷调查收集调查对象的人口学特征,通过体格测量收集调查对象的高血压患病等情况,并以晨尿检测结果为点尿钠水平估算24 h尿钠水平。采用SPSS 24.0进行t检验、方差分析、多重线性回归和多因素logistic回归分析。结果加权后,研究对象的高血压患病率为39.8%,单纯收缩期高血压患病率为19.7%,单纯舒张期高血压患病率为3.3%;收缩压中位数为134.00 mm Hg(P_(25)~P_(75):121.00~148.00 mm Hg),舒张压中位数为80.33 mm Hg(P_(25)~P_(75):72.33~88.33 mm Hg);24 h尿钠水平中位数为211.45 mmol/d(P_(25)~P_(75):173.13~254.36 mmol/d)。高血压、单纯收缩期高血压、单纯舒张期高血压患者24 h尿钠水平均高于相应的非患病人群,差异均有统计学意义(P<0.01)。多重线性回归分析调整相关因素后结果显示,24 h尿钠与收缩压(β=0.17)和舒张压(β=0.13)均呈正相关。多因素logistic回归分析结果显示,调整性别、年龄、教育程度、饮酒、吸烟、高血压家族史、BMI和糖尿病后,与24 h尿钠水平最低四分位数组相比,尿钠水平最高四分位数组患高血压、单纯收缩期高血压的风险较高,OR值分别为2.15(95%CI:1.74~2.65)、1.61(95%CI:1.25~2.08),均有统计学意义(P<0.01),单纯舒张期高血压的患病风险无统计学意义(OR=1.23,95%CI:0.73~2.08,P>0.05)。结论24 h尿钠水平与高血压、单纯收缩期高血压存在关联,应开展有效的干预和广泛的科普宣传,降低广大群众的食盐摄入量。
