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The Importance of Hair Goats in Turkey
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作者 Funda E. Atac Hakan Burcu 《Journal of Agricultural Science and Technology(A)》 2014年第4期364-369,共6页
Of the goat population in Turkey, 98% of the goat population consists of hair goats. They are the prime breeds of goats and they can be bred in the Mediterranean, Aegean and Southeast regions of Turkey. Hair goats are... Of the goat population in Turkey, 98% of the goat population consists of hair goats. They are the prime breeds of goats and they can be bred in the Mediterranean, Aegean and Southeast regions of Turkey. Hair goats are the main income for their breeders. The goats are typically raised in scarce conditions, thus giving rise to greater yield of meat. In spite of the fact that the studies are limited to hair goats bred by the public, in 2005, Republic of Turkey Ministry of Food, Agriculture and Livestock (TAGEM) initiated a countywide reclamation project aiming to identify the regional differences and characteristics of the hair goats. This paper focuses on the yearly quantity of hair goats' produce, particularly observing the proportions of goat milk, meat and other animal produce, and to highlight the importance of the hair goats. Recommendations are made from these findings. 展开更多
关键词 hair goat population MILK MEAT suggestions.
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Effects of Different Culture Condition in vitro on Growth of Cashmere Goat Primary Hair Follicles 被引量:4
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作者 李蘅 李金泉 +1 位作者 曹贵方 张文广 《Agricultural Science & Technology》 CAS 2008年第5期43-46,共4页
[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated un... [Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.[Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.[Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium. 展开更多
关键词 CASHMERE goat PRIMARY hair follicle Culture in VITRO
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Effects of Estradiol on Cashmere Goat Primary Hair Follicles Cultured in vitro 被引量:1
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作者 李蘅 李金泉 +1 位作者 曹贵方 张文广 《Agricultural Science & Technology》 CAS 2008年第6期90-92,共3页
[Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in ... [Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in serum-free Williams E media supplemented with different doses of 17 β-E2 (0, 0.1, 1.0, 10.0, 100.0 nmol/L), and their growth rates and morphological changes were observed. [Result] The growth rate of 0.1 nmol/L 17 β-E2 group was quite comparable with that of the control group(0 nmol/L), but the 17 β-E2 with concentrations of 1.0, 10.0 and 100.0 nmol/L displayed different degrees of inhibition on the growth of hair follicles. Different morphological changes of hair follicles could also be discovered in different concentration treatments. [Conclusion] The study laid a certain foundation for exploring the regulation mechanism of estrogen on growth of cashmere goat hair follicles. 展开更多
关键词 CASHMERE goat PRIMARY hair follicle Culture in VITRO 17 β-E2
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Technology of the Chemical Softening Process of Goat Hair
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作者 隋淑英 王培政 朱平 《Journal of China Textile University(English Edition)》 EI CAS 1998年第2期85-87,共3页
Under certain conditions,goat hair can be softened bychemicals.After the process,the structure of the macro-molecules in the surfacial area has been changed,and thehair has become soft,crimped and elastic.Thus,hairtha... Under certain conditions,goat hair can be softened bychemicals.After the process,the structure of the macro-molecules in the surfacial area has been changed,and thehair has become soft,crimped and elastic.Thus,hairthat can not be spun before the process can acquire agood spinnability.Woolen woven by processed hairs hasan unique style,of mohair blended fabrics.This articlemainly discusses the technology of the chemical softeningprocess of goat hair. 展开更多
关键词 goat hair CHEMICAL SofTENING process.
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Effects of the concentration and nature of total dissolved solids in drinking water on feed intake,nutrient digestion,energy balance,methane emission,ruminal fermentation,and blood constituents in different breeds of young goats and hair sheep
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作者 Amlan Kumar Patra Luana Paula dos Santos Ribeiro +5 位作者 Hirut Yirga Adekayode O.Sonibare Ahmed R.Askar Ali H.Hussein Ryszard Puchala Arthur Louis Goetsch 《Animal Nutrition》 SCIE CAS CSCD 2024年第1期84-95,共12页
Understanding how different livestock species and breeds respond to consumption of brackish water could improve usage of this resource.Therefore,Angora,Boer,and Spanish goat doelings and Dorper,Katahdin,and St.Croix e... Understanding how different livestock species and breeds respond to consumption of brackish water could improve usage of this resource.Therefore,Angora,Boer,and Spanish goat doelings and Dorper,Katahdin,and St.Croix ewe lambs(6 animals per animal type[AT];initial age=296±2.1 days)consuming water with varying concentrations of minerals of a natural brackish water source(BR)and sodium chloride(NaCl;SL)were used to determine effects on water and feed intake,nutrient digestion,heat energy,methane emission,ruminal fluid conditions,and blood constituent concentrations.There were 6 simultaneous 6(water treatments[WT])×6(AT)Latin squares with 3-wk periods.The WT were fresh(FR),BR alone(100-BR),a similar total dissolved solids(TDS)concentration as 100-BR via NaCl addition to FR(100-SL),BR with concentrations of all minerals increased by approximately 50%(150-BR),a similar TDS level as 150-BR by NaCl addition to FR(150-SL),and a similar 150 TDS level achieved by addition of a 1:1 mixture of BR minerals and NaCl to 100-BR(150-BR/SL).Concentrations(mg/kg)in BR were 4928 TDS,85.9 bicarbonate,224.9 calcium,1175 chloride,60.5 magnesium,4.59 potassium,1387sodium,1962 sulfate,and 8.3 boron,and TDS in other WT were 209,5684,7508,8309,and 7319 mg/kg for FR,100-SL,150-BR,150-SL,and 150-BR/SL,respectively.There were very few significant effects of WT or AT×WT interactions,although AT had numerous effects.Water intake was affected by AT(P=0.02)and WT(P=0.04),with greater water intake for 150-SL than for FR,100-BR,100-SL,and 150-BR.Dry matter intake among AT was lowest(P<0.05)for Angora.Digestion of organic matter and neutral detergent fiber and heat energy differed among AT(P<0.05),but nitrogen digestion and ruminal methane emission were similar among AT.Blood aldosterone concentration was higher(P<0.05)for FR than for other WT.In conclusion,all AT seemed resilient to these WT regardless of mineral source and concentrations,with TDS less than 8300 mg/kg,which did not influence nutrient utilization,ruminal fermentation,energy balance,or blood constituent levels. 展开更多
关键词 goats hair sheep Nutrient utilization Ruminal fermentation SALINITY Water quality
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Sulfur-encapsulated in heteroatom-doped hierarchical porous carbon derived from goat hair for high performance lithium–sulfur batteries 被引量:12
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作者 Juan Ren Yibei Zhou +3 位作者 Huali Wu Fengyu Xie Chenggang Xu Dunmin Lin 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2019年第3期121-131,共11页
Biomass-derived carbon materials have aroused widespread concern as host material of sulfur to enhance electrochemical performances for lithium–sulfur batteries. Herein, goat hair, as a low-cost and eco-friendly prec... Biomass-derived carbon materials have aroused widespread concern as host material of sulfur to enhance electrochemical performances for lithium–sulfur batteries. Herein, goat hair, as a low-cost and eco-friendly precursor, is employed to fabricate cauliflower-like in-situ nitrogen, oxygen and phosphorus tri-doped porous biomass carbon(NOPC) by a facile activation with H_3PO_4 and carbonization process.The morphology and microstructure of NOPC can be readily tuned by altering pyrolysis temperature. The as-prepared NOPC matrix material carbonized at 600 °C possesses 3D hierarchical porous structure, high specific surface area(535.352 m^2 g^(-1)), and appropriate pore size and pore size distribution. Encapsulating sulfur into the NOPC depends on a stem-melting technology as cathode materials of Li–S batteries. Due to the synergistic effect of special physical structure and inherent tri-doping of N, O and P, electrons and ions transfer and utilization of active sulfur in the materials are improved, and the shuttle behaviors of soluble lithium polysulfides are also mitigated. Consequently, the S/NOPC-600 composite exhibits excellent electrochemical performance, giving a high initial discharge capacity of 1185 mA h g^(-1) at 0.05 C and maintaining a relatively considerable capacity of 489 m A h g^(-1) at 0.2 C after 300 cycles. Our work shows that a promising candidate for cathode material of Li–S batteries can be synthesized using low-cost and renewable biomass materials by a facile process. 展开更多
关键词 Biomass-derived carbon materials goat hair Nitrogen Oxygen and phosphorus tri-doping LITHIUM SULFUR BATTERIES
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Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat 被引量:11
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作者 WU Jiang-hong ZHANG Yan-jun +5 位作者 ZHANG Jia-xin CHANG Zi-li LI Jin-quan YAN Zu-wei Husile ZHANG Wen-guang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第7期1159-1166,共8页
Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As a... Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxcl3 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFP- Hoxc13. The result suggested that overexpression ofHoxcl3 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression. 展开更多
关键词 Hoxcl3 Β-CATENIN Cashmere goat hair follicle activity CYCLE
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Hoxc13 Expression Pattern in Cashmere Goat Skin During Hair Follicle Development 被引量:12
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作者 WU Jiang-hong ZHANG Wen-guang +2 位作者 LI Jin-quan YIN Jun ZHANG Yan-jun 《Agricultural Sciences in China》 CAS CSCD 2009年第4期491-496,共6页
Hoxc13 has an important role in controlling hair formation. In this study, we examine the Hoxc13 RNA expression pattern of skin during embryo development. The result indicated that changes of the Hoxe13 gene expressio... Hoxc13 has an important role in controlling hair formation. In this study, we examine the Hoxc13 RNA expression pattern of skin during embryo development. The result indicated that changes of the Hoxe13 gene expression and thickness of skin have a similar trend during hair follicle morphogenesis. In interpreting these results, we investigated whether the regulation motifs is in Hoxc13 intron, which is a 5.4 kb fragment. To blast with other mammals, we found a very conservative region in all mammal animals and two regions in livestock, such as cow, sheep, horse, dog, and so on, which are not in other Hox genes. We have examined putative pre-miRNA in this region, providing an entry point for elucidating currently unknown mechanisms that are required for regulating quantitative levels of Hoxc13 gene expression. 展开更多
关键词 Hoxc13 gene expression Cashmere goat hair follicle
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半胱氨酸、蛋氨酸对体外培养绒山羊次级毛囊生长及毛乳头细胞增殖的影响 被引量:1
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作者 康佳 段香茹 +6 位作者 尹雪姣 杨若晨 李太春 单新雨 陈美静 张英杰 刘月琴 《畜牧兽医学报》 CAS CSCD 北大核心 2024年第2期515-527,共13页
旨在探讨半胱氨酸(Cys)、蛋氨酸(Met)对体外培养燕山绒山羊次级毛囊(SF)生长及毛乳头细胞(DPCs)增殖凋亡的影响。本研究选取1只1周岁左右健康雄性燕山绒山羊皮肤若干,完整分离的次级毛囊随机分成Cys试验组和Met试验组,每组各浓度3个重复... 旨在探讨半胱氨酸(Cys)、蛋氨酸(Met)对体外培养燕山绒山羊次级毛囊(SF)生长及毛乳头细胞(DPCs)增殖凋亡的影响。本研究选取1只1周岁左右健康雄性燕山绒山羊皮肤若干,完整分离的次级毛囊随机分成Cys试验组和Met试验组,每组各浓度3个重复,每个重复8根毛囊,Cys试验组添加浓度分别为0、40、80、120、160和200μg·mL^(-1);Met试验组添加浓度分别为0、10、15、20、25和30μg·mL^(-1)。毛囊培养7 d,每隔24 h观察毛囊生长形态并测量毛囊生长长度。从燕山绒山羊次级毛囊中提取、纯化DPCs,细胞计数并绘制DPCs生长曲线,将DPCs随机分为Cys试验组和Met试验组,每组各浓度6个重复,Cys试验组添加浓度分别为0、20、40、60、80、100μg·mL^(-1);Met试验组添加浓度分别为0、2、4、6、8、10μg·mL^(-1)。培养2 h后用CCK-8试剂盒进行细胞增殖活力测定,确定燕山绒山羊DPCs中Cys和Met的最适培养浓度,利用qRT-PCR技术检测不同浓度Cys和Met的DPCs增殖相关基因(PCNA、CCND1、CDC42、CDK 4)、凋亡相关基因(P21、P53、Bax、Caspase-3、Bcl-2)、皮肤细胞分化相关基因(IVL)及角蛋白相关基因(K10、K 14)mRNA表达水平。结果表明,与对照组相比,添加80和120μg·mL^(-1) Cys与10、15、20和25μg·mL^(-1) Met均可显著影响次级毛囊生长速率和累计生长长度(P<0.01),且120μg·mL^(-1) Cys和20μg·mL^(-1) Met促生长效果最好;添加20、40和60μg·mL^(-1) Cys可以显著影响DPCs增殖(P<0.05),添加40μg·mL^(-1) Cys显著上调PCNA、CCND1、CDK4、Bcl-2、K10、K 14和IVL基因mRNA表达量(P<0.05);添加6μg·mL^(-1) Met可以显著影响DPCs增殖(P<0.05),并显著上调PCNA、CCND1、CDK4、P21、Bcl-2、K 10和K 14基因mRNA表达量(P<0.05)。综上,添加Cys和Met可以显著促进长绒期燕山绒山羊次级毛囊生长,最适添加量分别为120μg·mL^(-1)和20μg·mL^(-1);添加Cys和Met可通过上调细胞增殖、分化、角蛋白等基因mRNA、下调细胞凋亡基因mRNA表达,促进乳头细胞增殖、抑制细胞凋亡进而促进燕山绒山羊次级毛囊生长。 展开更多
关键词 半胱氨酸 蛋氨酸 绒山羊 次级毛囊 毛乳头细胞 基因表达
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绒毛用羊毛囊干细胞研究进展
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作者 宋亚丽 卢曾奎 +3 位作者 袁超 杨博辉 刘建斌 郭婷婷 《中国草食动物科学》 CAS 北大核心 2024年第2期73-76,共4页
产毛(绒)量是绒毛用羊主要的经济性状,毛(绒)的质量和产量受毛囊发育的影响。众所周知,毛囊控制羊毛(绒)的生长,而毛囊干细胞决定着毛囊形态的发生。因此,文章从绒毛用羊毛囊干细胞的分离培养方法、鉴定、增殖与分化等方面的相关研究进... 产毛(绒)量是绒毛用羊主要的经济性状,毛(绒)的质量和产量受毛囊发育的影响。众所周知,毛囊控制羊毛(绒)的生长,而毛囊干细胞决定着毛囊形态的发生。因此,文章从绒毛用羊毛囊干细胞的分离培养方法、鉴定、增殖与分化等方面的相关研究进展进行了综合阐述,为进一步开展毛囊生长发育的分子调控机制研究、选育绒毛性状优良的绒毛生产用羊提供参考资料。 展开更多
关键词 羊绒 羊毛 毛囊 毛囊干细胞
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拉毛针织面料热传递性能的有限元仿真
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作者 刘薇 雷敏 +2 位作者 沈华 李毓陵 马颜雪 《现代纺织技术》 北大核心 2024年第8期100-107,共8页
为探究拉毛针织面料的毛羽对面料热传递性能的影响规律,提出了一种预测拉毛针织面料热传递性能的方法,建立了针织线圈-毛羽层模型,在此基础上对拉毛针织面料的热传递性能进行有限元仿真分析,并通过实验验证模型的有效性。首先对实验面... 为探究拉毛针织面料的毛羽对面料热传递性能的影响规律,提出了一种预测拉毛针织面料热传递性能的方法,建立了针织线圈-毛羽层模型,在此基础上对拉毛针织面料的热传递性能进行有限元仿真分析,并通过实验验证模型的有效性。首先对实验面料进行测量,得到织物的几何结构参数,利用SolidWorks专业建模软件建立织物的针织线圈结构,利用ANSYS DesignModeler建立织物的毛羽层;然后借助有限元分析软件ANSYS Fluent,根据模拟环境设置边界条件,进行迭代运算,得到该模型的温度分布云图;最后将仿真模拟的结果与实验测试结果进行对比,对模型的有效性进行验证。研究结果表明:拉毛针织面料的模拟热阻值与实验热阻值相关系数接近0.993,最大差异小于17.7%,表明该有限元模型具有一定的可行性;拉毛针织面料的表面毛羽密度越高,织物的热阻值越大,但是毛羽密度达到一定范围后,织物热阻值发生较小变化。 展开更多
关键词 热传递 毛羽 拉毛针织面料 有限元仿真 线圈模型
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基于RNA测序技术鉴定和分析绒山羊毛囊不同生长期mRNA的可变剪接
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作者 李莹莹 董华娇 +6 位作者 梁千千 张洁 李甜甜 许赫 马月辉 韩吉龙 杨敏 《中国草食动物科学》 CAS 北大核心 2024年第4期1-7,共7页
可变剪接(Alternative splicing,AS)是在转录后水平调控基因表达的一种重要方式。为挖掘调控绒山羊毛囊周期性生长相关的关键可变剪接,通过比较绒山羊绒毛不同生长时期的皮肤转录组数据,以P<0.05,|Inc‐LevelDifference|>0.1为标... 可变剪接(Alternative splicing,AS)是在转录后水平调控基因表达的一种重要方式。为挖掘调控绒山羊毛囊周期性生长相关的关键可变剪接,通过比较绒山羊绒毛不同生长时期的皮肤转录组数据,以P<0.05,|Inc‐LevelDifference|>0.1为标准筛选差异的可变剪接,并进行功能分析。结果显示,转换期Ⅰ、Ⅱ、Ⅲ、Ⅳ中分别鉴定到1615、1667、1387和1522个差异可变剪接,分别对应了1202、1268、1073、1170个基因;这些基因主要富集于Notch、Hedgehog、mTOR、AMPK、PI3K-Akt、Wnt、TGF-β等与毛囊发育相关的信号通路;蛋白互作网络结合富集分析表明,Ctnnb1、Gsk3b、Tgfbr1、Vegfa、Gli1等基因产生的可变剪接可能参与调控毛囊周期性生长。综上可知,可变剪接在绒山羊毛囊周期性生长过程中普遍存在,且在不同发育阶段间存在差异;鉴定到调控绒山羊毛囊周期性生长发育相关的关键差异可变剪接基因及其重要信号通路。 展开更多
关键词 绒山羊 毛囊周期性生长 可变剪接 转录组测序
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基于转录组测序筛选疆南绒山羊次级毛囊周期发育相关的circRNA
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作者 陆庆伟 唐森 +6 位作者 李彬 秦崇凯 刘文娜 蒲雪 王亚倩 吴翠玲 付雪峰 《中国畜牧兽医》 CAS CSCD 北大核心 2024年第5期1793-1806,共14页
【目的】探讨疆南绒山羊皮肤毛囊的生长发育机制,加深对次级毛囊发育相关候选基因遗传调控和功能的认识,为指导疆南绒山羊后期培育,改善其绒产量提供参考。【方法】利用高通量测序技术对疆南绒山羊次级毛囊生长期、退行期和休止期皮肤... 【目的】探讨疆南绒山羊皮肤毛囊的生长发育机制,加深对次级毛囊发育相关候选基因遗传调控和功能的认识,为指导疆南绒山羊后期培育,改善其绒产量提供参考。【方法】利用高通量测序技术对疆南绒山羊次级毛囊生长期、退行期和休止期皮肤组织中环状RNA(circRNA)进行分析,筛选出不同比较组的差异表达circRNA(DE circRNA),对其宿主基因进行GO功能、KEGG通路及蛋白互作(PPI)分析,并联合前期转录组数据构建竞争性内源RNA(ceRNA)网络。【结果】在9个疆南绒山羊皮肤组织中共鉴定到2482个circRNAs。其中包含138个DE circRNAs,退行期和生长期、退行期和休止期、休止期和生长期比较组中分别存在54、74、65个DEcircRNAs。GO功能和KEGG通路分析揭示,DEcircRNA宿主基因主要富集在细胞黏附、整合素介导信号通路、细胞整合素复合物等功能通路,以及一些与毛囊生理过程有关的信号通路,如MAPK、TGF-beta、Hedgehog等。结合PPI网络推测14个circRNAs可能参与调控疆南绒山羊皮肤次级毛囊生长发育。通过6个DEcircRNAs、20个DE miRNAs、3个DEmRNAs和2个DElncRNAs构建了疆南绒山羊ceRNA网络。【结论】本研究通过转录组测序筛选出与疆南绒山羊次级毛囊周期发育相关的关键circRNAs,包括novel-gene5238-2、novel-gene7855-1、novel-gene4455-1等。研究结果为circRNA在绒山羊次级毛囊发育中的生物学功能和调控特性提供理论依据,也为疆南绒山羊育种提供了新的方向。 展开更多
关键词 疆南绒山羊 转录组测序 环状RNA(circRNA) 次级毛囊周期
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ZB416A包装机铝箔纸润滑装置改造研究
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作者 钟世乐 刘谦 +1 位作者 黄春赟 张金岭 《绿色包装》 2024年第4期33-36,共4页
针对ZB416A包装机组的铝箔纸润滑不均匀,涂油装置安装在输送辊与切刀辊对之间,油量大小难以把控,对产品的内在质量产生安全隐患。通过对工作原理及结构进行分析,利用气缸伸缩的工作原理对关键部件进行改造,将原来的喷雾式涂油装置改造... 针对ZB416A包装机组的铝箔纸润滑不均匀,涂油装置安装在输送辊与切刀辊对之间,油量大小难以把控,对产品的内在质量产生安全隐患。通过对工作原理及结构进行分析,利用气缸伸缩的工作原理对关键部件进行改造,将原来的喷雾式涂油装置改造为伸缩毛刷式涂油装置。经过改造后,烟包盒内衬纸涂油均匀,折叠良好,避免了因内衬纸涂油不均匀而导致的卷烟内部缺陷。同时减少了维修人员的工作量和机台操作人员的劳动强度,减少维修耗时,提升产品质量,达到提质增效的目的。 展开更多
关键词 ZB416A包装机 铝箔纸 毛刷 气缸
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羊胎儿期皮肤毛囊发育及调控机制的研究概述 被引量:2
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作者 秦志云 姜怀志 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第2期638-646,共9页
中国绵山羊养殖历史悠久,品种资源丰富,在国民经济中起重要作用。羊毛按纤维类型可分为同质毛和异质毛,同质毛的特点是被毛由同一类型的羊毛纤维组成,其特点是羊毛的纤维直径、长度、卷曲等外表特征基本相同,异质毛的特点是被毛中兼有... 中国绵山羊养殖历史悠久,品种资源丰富,在国民经济中起重要作用。羊毛按纤维类型可分为同质毛和异质毛,同质毛的特点是被毛由同一类型的羊毛纤维组成,其特点是羊毛的纤维直径、长度、卷曲等外表特征基本相同,异质毛的特点是被毛中兼有绒毛、有髓毛、无卷曲和少卷毛等不同类型的羊毛纤维,其化学、物理性能均不相同。毛囊是控制哺乳动物被毛生长的重要器官,是唯一具有周期性发育并可终生再生的器官,控制着被毛发生发育与自然脱落。毛囊依据其形成时间和结构可分为初级毛囊(primary follicles)和次级毛囊(secondary follicles),初级毛囊生长髓质发达的粗毛,次级毛囊产生无髓毛的绒毛。有髓毛较粗长且弯曲少,多用于加工粗纺织品、毛毯、地毯、毡制品;无髓毛直径一般不超40μm,弯曲多,是毛纺工业的优质原料。毛囊的发育受到不同信号通路和基因的影响,如Wnt信号通路、骨形态发生蛋白质(BMP)信号通路、成纤维细胞生长因子(FGF)信号通路、音猬因子(SHH)信号通路、Notch信号通路等以及角蛋白家族基因、BMPs家族基因、同源异型盒基因(Hox)等。作者对国内外对绵山羊胎儿期皮肤毛囊发育及调控机制进行综述,以期为提高以绵山羊被毛产量及改善被毛品质为主要育种目标的选育提供参考。 展开更多
关键词 绵山羊 胎儿期 毛囊发育 调控机制
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CSDC2蛋白免疫优势肽段分析及其多克隆抗体的制备
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作者 朱荷青 玛哈巴·肉孜 +6 位作者 李莹莹 张洁 许赫 李甜甜 韩吉龙 彭夏雨 杨敏 《石河子大学学报(自然科学版)》 CAS 北大核心 2023年第2期193-198,共6页
目的为制备含有C2的冷休克结构域(cold shock domain containing C2,CSDC2)的特异性抗体,本试验预测了CSDC2蛋白的免疫优势肽段并制备多克隆抗体,可为进一步探究其功能奠定基础。方法本研究以绒山羊CSDC2蛋白全长氨基酸序列为基础,应用... 目的为制备含有C2的冷休克结构域(cold shock domain containing C2,CSDC2)的特异性抗体,本试验预测了CSDC2蛋白的免疫优势肽段并制备多克隆抗体,可为进一步探究其功能奠定基础。方法本研究以绒山羊CSDC2蛋白全长氨基酸序列为基础,应用在线分析工具Expasy分析亲疏水性;SOPMA和Predictprotein共同分析二级结构;DNAstar分析抗原指数、表面可及性、柔韧性;TMHHM 2.0预测跨膜结构;SWISS-MODEL预测三级结构;SignalP 5.0预测信号肽;NetPhos 3.1预测磷酸化位点;SVMTriP在线分析软件预测抗原表位,综上分析获得CSDC2免疫优势肽段,制备多克隆抗体;ELISA检测多克隆抗体效价,Western blot验证抗体特异性。结果CSDC2为不稳定亲水蛋白,无跨膜结构和信号肽区域,具有22个磷酸化位点,二级结构主要由无规则卷曲、α-螺旋和延伸链构成。最终选取绒山羊CSDC2蛋白N-端15-33氨基酸序列(HSPKSPVWPTFPFHREGSR)为特异免疫肽段,诱导兔产生CSDC2蛋白特异性IgG抗体,Western blot结果表明该抗体可以与绒山羊CSDC2蛋白进行特异性结合。结论本研究得出绒山羊CSDC2的免疫优势肽段为15~33 aa(HSPKSPVWPTFPFHREGSR)并免疫试验兔制备了多克隆抗体,进一步实验证明该抗体具有较好生物学效价,为后续深入研究CSDC2在绒山羊绒毛周期性生长调控的作用奠定了理论基础。 展开更多
关键词 绒山羊 CSDC2 生物信息学分析 毛囊周期性生长 多克隆抗体
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COL 1A1在绒山羊次级毛囊生长周期中的表达模式 被引量:1
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作者 冯娟 王东 +5 位作者 王笑冰 张茹 夏慧岩 常嘉乐 刘雨静 肖红梅 《兽医导刊》 2023年第1期1-5,共5页
COL1A1为I型α1胶原蛋白(Collagen type I alpha 1),在动物体内广泛存在。为了解其在绒山羊次级毛囊生长周期中的表达模式,利用实时荧光定量PCR技术、免疫组化技术、蛋白质印迹技术,从基因和蛋白水平分别对次级毛囊生长3个不同时期的COL... COL1A1为I型α1胶原蛋白(Collagen type I alpha 1),在动物体内广泛存在。为了解其在绒山羊次级毛囊生长周期中的表达模式,利用实时荧光定量PCR技术、免疫组化技术、蛋白质印迹技术,从基因和蛋白水平分别对次级毛囊生长3个不同时期的COL1A1基因表达量及表达差异、表达部位进行了检测。结果表明,COL1A1基因在绒山羊次级毛囊的生长期(9月)表达量最高,退行期(12月)表达量其次,休止期(2月)表达量相对最低。COL1A1主要表达于次级毛囊的外根鞘。由此说明,COL1A1通过调控绒山羊次级毛囊生长从而促进绒毛的生长。 展开更多
关键词 绒山羊 COL1A1 次级毛囊 免疫组化 蛋白质印迹
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Production of transgenic cashmere goat embryos expressing red fluorescent protein and containing IGF1 hair-follicle-cell specific expression cassette by somatic cell nuclear transfer 被引量:2
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作者 BOU ShorGan 《Science China(Life Sciences)》 SCIE CAS 2009年第4期390-397,共8页
In the present study, cashmere goat fetal fibroblasts were transfected with pCDsR-KI, a hair-follicle-cell specific expression vector for insulin-like growth factor 1 (IGF1) that contains two markers for selection (re... In the present study, cashmere goat fetal fibroblasts were transfected with pCDsR-KI, a hair-follicle-cell specific expression vector for insulin-like growth factor 1 (IGF1) that contains two markers for selection (red fluorescent protein gene and neomycin resistant gene). The transgenic fibroblasts cell lines were obtained after G418 selection. Prior to the somatic cell nuclear transfer (SCNT), the maturation rate of caprine cumulus oocytes complexes (COCs) was optimized to an in vitro maturation time of 18 h. Parthenogenetic ooctyes were used as a model to investigate the effect of two activation methods, one with calcium ionophore IA23187 plus 6-DMAP and the other with ethanol plus 6-DMAP. The cleavage rates after 48 h were respectively 88.7% and 86.4%, with no significant difference (P>0.05). There was no significant difference between the cleavage rate and the blastocyst rate in two different media (SO- Faa and CR1aa; 86.3% vs 83.9%, P>0.05 and 23.1% vs 17.2%,P>0.05). The fusion rate of a 190 V/mm group (62.4%) was significantly higher than 130 V/mm (32.8%) and 200 V/mm (42.9%), groups (P<0.05). After transgenic somatic cell nuclear transfer (TSCNT) manipulation, 203 reconstructed embryos were obtained in which the cleavage rate after in vitro development (IVD) for 48 h was 79.3% (161/203). The blastocyst rate after IVD for 7 to 9 d was 15.3% (31/203). There were 17 embryos out of 31 strongly ex- pressing red fluorescence. Two of the red fluorescent blastocysts were randomly selected to identify transgene by polymerase chain reaction. Both were positive. These results showed that: (i) RFP and Neor genes were correctly expressed indicating that transgenic somatic cell lines and positive trans- genic embryos were obtained; (ii) one more selection at the blastocyst stage was necessary although the donor cells were transgenic positive, because only partially transgenic embryos expressing red fluorescence were obtained; and (iii) through TSCNT manipulation and optimization, transgenic cash- mere goat embryos expressing red fluorescence and containing an IGF1 expression cassette were obtained, which was sufficient for production of transgenic cashmere goats. 展开更多
关键词 CASHMERE goat insulin like growth factor 1 hair follicle fetal fibroblasts cells TRANSGENE SOMATIC cell nuclear transfer
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A Single-cell Transcriptome Atlas of Cashmere Goat Hair Follicle Morphogenesis 被引量:4
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作者 Wei Ge Weidong Zhang +10 位作者 Yuelang Zhang Yujie Zheng Fang Li Shanhe Wang Jinwang Liu Shaojing Tan Zihui Yan Lu Wang Wei Shen Lei Qu Xin Wang 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2021年第3期437-451,共15页
Cashmere,also known as soft gold,is produced from the secondary hair follicles(SHFs)of cashmere goats.The number of SHFs determines the yield and quality of cashmere;therefore,it is of interest to investigate the tran... Cashmere,also known as soft gold,is produced from the secondary hair follicles(SHFs)of cashmere goats.The number of SHFs determines the yield and quality of cashmere;therefore,it is of interest to investigate the transcriptional profiles present during cashmere goat hair follicle development.However,mechanisms underlying this development process remain largely unexplored,and studies regarding hair follicle development mostly use a murine research model.In this study,to provide a comprehensive understanding of cellular heterogeneity and cell fate decisions,single-cell RNA sequencing was performed on 19,705 single cells of the dorsal skin from cashmere goat fetuses at induction(embryonic day 60;E60),organogenesis(E90),and cytodifferentiation(E120)stages.For the first time,unsupervised clustering analysis identified 16 cell clusters,and their corresponding cell types were also characterized.Based on lineage inference,a detailed molecular landscape was revealed along the dermal and epidermal cell lineage developmental pathways.Notably,our current data also confirmed the heterogeneity of dermal papillae from different hair follicle types,which was further validated by immunofluorescence analysis.The current study identifies different biomarkers during cashmere goat hair follicle development and has implications for cashmere goat breeding in the future. 展开更多
关键词 Single-cell transcriptome Cashmere goat Cellular heterogeneity Developmental trajectory hair follicle morphogenesis
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内蒙古绒山羊与辽宁绒山羊皮肤毛囊周期性变化的比较研究 被引量:50
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作者 李长青 尹俊 +6 位作者 张燕军 郭志成 张文广 高爱琴 李玉荣 赖双英 李金泉 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第7期674-679,共6页
对内蒙古阿尔巴斯白绒山羊和辽宁绒山羊皮肤及毛囊的组织学周期性变化作了比较研究,结果表明:表皮生发层细胞从3月份开始活动并向下延伸,此时毛囊也开始重建,绒山羊毛囊8—9月份进入兴盛期,12月份进入退行期,2—3月份为休止期。它们进... 对内蒙古阿尔巴斯白绒山羊和辽宁绒山羊皮肤及毛囊的组织学周期性变化作了比较研究,结果表明:表皮生发层细胞从3月份开始活动并向下延伸,此时毛囊也开始重建,绒山羊毛囊8—9月份进入兴盛期,12月份进入退行期,2—3月份为休止期。它们进入兴盛期和持续的时间不同,初级毛囊除毛球宽外,其它各性状阿尔巴斯白绒山羊均大于辽宁绒山羊,而次级毛囊各性状辽宁绒山羊都大于阿尔巴斯白绒山羊,在大多数月份二者差异显著。毛囊深、密度、S/P、毛球宽和生长期是影响产绒量的因素。 展开更多
关键词 内蒙古绒山羊 辽宁绒山羊 毛囊 周期性
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