Folk activities are the most representative form of regional culture,the humanistic care,homeland affection and festival customs that run through them are all vivid and true embodiments of local life.The folk custom a...Folk activities are the most representative form of regional culture,the humanistic care,homeland affection and festival customs that run through them are all vivid and true embodiments of local life.The folk custom activity of"Zhang and Li combined households"is a living inheritance of the historical fact of Mongolian-Han fusion in Taomin region.It takes Shehuo(festival entertainment)as the carrier,and uses rich forms of performance to recognize relatives and worship ancestors.At the same time,as a kind of entertainment and catharsis,it also carries the social function of folk culture,such as maintaining and connecting the emotions between the ethnic groups and neighbors,etc.On the basis of field investigation,this paper deeply describes the folk custom event of"Zhang and Li combined households",shows the whole picture of the grand festival,and discusses the ancestral memory,identifies and ethnic integration process of"Zhang and Li combined households"as a cultural phenomenon.展开更多
Lin Yutang does a great deal in translating and introducing Chinese Classical literature to western countries,yet he also makes many mistakes in the translation and introduction. This essay talks about his three error...Lin Yutang does a great deal in translating and introducing Chinese Classical literature to western countries,yet he also makes many mistakes in the translation and introduction. This essay talks about his three errors. First,he misunderstands the functions of fa(法) in Confueianism. Next,he mistranslates some sayings such as Fafenwangshi(“发愤忘食”), so he distorts the image of Confucius. Last,he doesnot understand the meaning of Sanshierli(“三十而立”)correctly,so that he ignores the important role of li(礼) in Confucius′ whole life.展开更多
目的研究绞股蓝皂苷LI(Gyp LI)与顺铂(DDP)联用对食管癌EC109细胞存活的抑制作用,以及对细胞凋亡、周期和迁移的影响。方法(1)将Gyp LI 0~200μmol·L^(-1)、DDP 0~800μmol·L^(-1)、Gyp LI3.125μmol·L^(-1)+DDP 0~200μ...目的研究绞股蓝皂苷LI(Gyp LI)与顺铂(DDP)联用对食管癌EC109细胞存活的抑制作用,以及对细胞凋亡、周期和迁移的影响。方法(1)将Gyp LI 0~200μmol·L^(-1)、DDP 0~800μmol·L^(-1)、Gyp LI3.125μmol·L^(-1)+DDP 0~200μmol·L^(-1)分别作用EC109细胞24 h,MTT法检测细胞存活率并计算半抑制浓度(IC_(50))值,利用CompuSyn软件计算联合指数(CI)。(2)将EC109细胞分为细胞对照组、Gyp LI60μmol·L^(-1)组、DDP 35μmol·L^(-1)组及Gyp LI 3.125μmol·L^(-1)和DDP 10μmol·L^(-1)联用组,给药24 h后,显微镜下观察细胞形态,Annexin V-FITC/PI双染、PI/RNase染色和流式细胞术检测细胞凋亡率和细胞周期,采用划痕实验检测细胞迁移,Western印迹法检测促凋亡因子Bax、细胞色素c、细胞周期蛋白A、细胞周期蛋白D1和基质金属蛋白酶9(MMP-9)蛋白表达。结果(1)Gyp LI和DDP单用组EC109细胞存活率的IC_(50)值分别为(55.34±3.52)μmol·L^(-1)和(37.48±2.99)μmol·L^(-1),Gyp LI 3.125μmol·L^(-1)与不同浓度DDP联用时,IC_(50)值降至(8.05±5.34)μmol·L^(-1),CI值均<1,表明两药联用具有协同作用。(2)Gyp LI与DDP联用时细胞出现生长缓慢、形态不规则、细胞脱落等特征。与细胞对照组比较,Gyp LI 60μmol·L^(-1)组、DDP35μmol·L^(-1)组和联用组的细胞凋亡率从(1.30±0.08)%分别上升至(4.87±0.04)%,(6.44±0.37)%和(9.12±0.20)%(P<0.01),联用组细胞凋亡率比DDP单用组显著升高(P<0.01)。Gyp LI组、DDP组和联用组细胞周期分别阻滞于S期,G_(2)/M期和G_(0)/G_(1)期。与细胞对照组比较,Gyp LI 60μmol·L^(-1)组、DDP 35μmol·L^(-1)组和联用组细胞迁移率从(29.54±5.56)%分别降至(15.30±3.64)%,(13.61±0.06)%和(5.95±0.56)%(P<0.01)。Western印迹法结果显示,与细胞对照组相比,DDP 35μmol·L^(-1)组和联用组促凋亡因子Bax表达水平均显著升高(P<0.01),Gyp LI 60μmol·L^(-1)组、DDP 35μmol·L^(-1)组和联用组细胞色素c表达水平均显著升高(P<0.01),其中联用组Bax和细胞色素c相对表达含量最高;与细胞对照组相比,Gyp LI 60μmol·L^(-1)组细胞周期蛋白A表达水平显著降低(P<0.01),联用组细胞周期蛋白D1表达水平显著降低(P<0.05);与细胞对照组相比,Gyp LI 60μmol·L^(-1)组、DDP 35μmol·L^(-1)组和联用组MMP-9表达均下降(P<0.05,P<0.01),其中联用组MMP-9表达水平最低。结论Gyp LI具有较好的抑制EC109细胞存活作用,与DDP联用能够协同抑制EC109细胞增殖,联用机制可能与上调Bax和细胞色素c表达、下调细胞周期蛋白D1和MMP-9表达有关。展开更多
文摘Folk activities are the most representative form of regional culture,the humanistic care,homeland affection and festival customs that run through them are all vivid and true embodiments of local life.The folk custom activity of"Zhang and Li combined households"is a living inheritance of the historical fact of Mongolian-Han fusion in Taomin region.It takes Shehuo(festival entertainment)as the carrier,and uses rich forms of performance to recognize relatives and worship ancestors.At the same time,as a kind of entertainment and catharsis,it also carries the social function of folk culture,such as maintaining and connecting the emotions between the ethnic groups and neighbors,etc.On the basis of field investigation,this paper deeply describes the folk custom event of"Zhang and Li combined households",shows the whole picture of the grand festival,and discusses the ancestral memory,identifies and ethnic integration process of"Zhang and Li combined households"as a cultural phenomenon.
文摘Lin Yutang does a great deal in translating and introducing Chinese Classical literature to western countries,yet he also makes many mistakes in the translation and introduction. This essay talks about his three errors. First,he misunderstands the functions of fa(法) in Confueianism. Next,he mistranslates some sayings such as Fafenwangshi(“发愤忘食”), so he distorts the image of Confucius. Last,he doesnot understand the meaning of Sanshierli(“三十而立”)correctly,so that he ignores the important role of li(礼) in Confucius′ whole life.
基金supports from the National Natural Science Foundation of China(Nos.52204268,51974365,91962223)the Natural Science Foundation of Hunan Province,China(Nos.2020JJ5747,2020JJ5749)+3 种基金the Innovation-driven Project of Central South University,China(No.2020CX039)the National“111”Project of China(No.B14034)the Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources,China(No.2018TP1002)the Young Elite Scientists Sponsorship Program by CAST,China(No.2019QNRC001).
文摘目的研究绞股蓝皂苷LI(Gyp LI)与顺铂(DDP)联用对食管癌EC109细胞存活的抑制作用,以及对细胞凋亡、周期和迁移的影响。方法(1)将Gyp LI 0~200μmol·L^(-1)、DDP 0~800μmol·L^(-1)、Gyp LI3.125μmol·L^(-1)+DDP 0~200μmol·L^(-1)分别作用EC109细胞24 h,MTT法检测细胞存活率并计算半抑制浓度(IC_(50))值,利用CompuSyn软件计算联合指数(CI)。(2)将EC109细胞分为细胞对照组、Gyp LI60μmol·L^(-1)组、DDP 35μmol·L^(-1)组及Gyp LI 3.125μmol·L^(-1)和DDP 10μmol·L^(-1)联用组,给药24 h后,显微镜下观察细胞形态,Annexin V-FITC/PI双染、PI/RNase染色和流式细胞术检测细胞凋亡率和细胞周期,采用划痕实验检测细胞迁移,Western印迹法检测促凋亡因子Bax、细胞色素c、细胞周期蛋白A、细胞周期蛋白D1和基质金属蛋白酶9(MMP-9)蛋白表达。结果(1)Gyp LI和DDP单用组EC109细胞存活率的IC_(50)值分别为(55.34±3.52)μmol·L^(-1)和(37.48±2.99)μmol·L^(-1),Gyp LI 3.125μmol·L^(-1)与不同浓度DDP联用时,IC_(50)值降至(8.05±5.34)μmol·L^(-1),CI值均<1,表明两药联用具有协同作用。(2)Gyp LI与DDP联用时细胞出现生长缓慢、形态不规则、细胞脱落等特征。与细胞对照组比较,Gyp LI 60μmol·L^(-1)组、DDP35μmol·L^(-1)组和联用组的细胞凋亡率从(1.30±0.08)%分别上升至(4.87±0.04)%,(6.44±0.37)%和(9.12±0.20)%(P<0.01),联用组细胞凋亡率比DDP单用组显著升高(P<0.01)。Gyp LI组、DDP组和联用组细胞周期分别阻滞于S期,G_(2)/M期和G_(0)/G_(1)期。与细胞对照组比较,Gyp LI 60μmol·L^(-1)组、DDP 35μmol·L^(-1)组和联用组细胞迁移率从(29.54±5.56)%分别降至(15.30±3.64)%,(13.61±0.06)%和(5.95±0.56)%(P<0.01)。Western印迹法结果显示,与细胞对照组相比,DDP 35μmol·L^(-1)组和联用组促凋亡因子Bax表达水平均显著升高(P<0.01),Gyp LI 60μmol·L^(-1)组、DDP 35μmol·L^(-1)组和联用组细胞色素c表达水平均显著升高(P<0.01),其中联用组Bax和细胞色素c相对表达含量最高;与细胞对照组相比,Gyp LI 60μmol·L^(-1)组细胞周期蛋白A表达水平显著降低(P<0.01),联用组细胞周期蛋白D1表达水平显著降低(P<0.05);与细胞对照组相比,Gyp LI 60μmol·L^(-1)组、DDP 35μmol·L^(-1)组和联用组MMP-9表达均下降(P<0.05,P<0.01),其中联用组MMP-9表达水平最低。结论Gyp LI具有较好的抑制EC109细胞存活作用,与DDP联用能够协同抑制EC109细胞增殖,联用机制可能与上调Bax和细胞色素c表达、下调细胞周期蛋白D1和MMP-9表达有关。
