Contents of soil organic carbon and nitrogen in water-stable aggregates in abandoned agricultural lands in an arid ecosystem of Northwest China

Soil organic matter content in water-stable aggregates（WSA） in the arid ecosystems（abandoned agricultural lands especially） of China is poorly understood. In this study, we examined the WSA sizes and stability, and soil organic carbon（OC） and nitrogen（N） contents in agricultural lands with abandonment ages of 0, 3, 12, 20, 30 and 40 years, respectively, in the Minqin Oasis of Northwest China. The total soil OC and N contents at depths of 0–20, 20–40 and 40–60 cm in abandoned agricultural lands were compared to those in cultivated land（the control）. Agricultural land abandonment significantly（P0.25 mm） as the age of agricultural land abandonment increased. The effect of abandonment ages of agricultural lands on MWD was determined by the changes of OC and N accumulation in WSA sizes ＆gt;2 mm. The total OC and N contents presented a stratification phenomenon across soil depths in this arid ecosystem. That is, both of them decreased significantly at depths of 0–20 and 40–60 cm while increased at the depth of 20–40 cm. The WSA sizes ＆lt;0.053 mm had the highest soil OC and N contents（accounting for 51.41%–55.59% and 42.61%–48.94% of their total, respectively）. Soil OC and N contents in microaggregates（sizes 0.053–0.25 mm） were the dominant factors that influenced the variations of total OC and N contents in abandoned agricultural lands. The results of this study suggested that agricultural land abandonment may result in the recovery of WSA stability and the shifting of soil organic matter from the silt＋clay（＆lt;0.053 mm） and microaggregate fractions to the macroaggregate fractions. However, agricultural land abandonment did not increase total soil OC and N contents in the short-term.

Effects of Long-Term Fertilization on the Distribution of Carbon, Nitrogen and Phosphorus in Water-Stable Aggregates in Paddy Soil

We investigated the size distribution of water-stable aggregates and the soil carbon, nitrogen and phosphorus concentration over aggregate size fractions based on a long-term （1990-2006） fertilization experiment in a reddish paddy soil. The results showed that the largest water-stable aggregate （WSA） （〉5 mm） and the smallest WSA （〈0.25 mm） took up the first largest proportion （38.3%） and the second largest proportion （23.3%）, respectively. Application of organic materials increased the proportion of the large WSA （〉2 mm） and decreased the proportion of the small WSA （〈1 ram）, resulting in an increase in the mean weight diameter of WSA, whereas application of chemical fertilizer had little effect. Application of organic materials, especially combined with chemical fertilizers, increased total carbon, nitrogen and phosphorus concentrations in all sizes of WSA, and total carbon, nitrogen and phosphorus were prone to concentrate in the large WSA. Further more, application of organic materials improved the supply effectiveness of available phosphorus, whereas had little influence on the labile carbon in WSA. Application of chemical fertilizers improved concentrations of total and available phosphorus in all sizes of WSA, whereas had little influence on total carbon and nitrogen contents. Economical fertilization model maintained the soil fertility when compared with full dose of chemical fertilizers, indicating that using organic materials could reduce chemical fertilizers by about one third.

Relationship Between Water-Stable Aggregates and Nutrients in Black Soils After Reclamation

水马厩总数，是为土壤的结构的性质的评估的一个索引，被许多因素影响。Zhaoguang 农场， Longzhen 农场，和 Jiusan 农场在黑土壤的区域被选择为代表性的学习地点，在东北中国的一个典型土壤资源。在有在黑色的营养素的 0.25 个公里水马厩总数和它的关系玷污的 】 的内容的变化在开垦的不同的年以后被调查。结果证明 0.25 公里水马厩聚集的 】 比在表面下的土壤是在表面的更多，他们在下列顺序变化了：Longzhen 农场 】 Zhaoguang 农场 】 Jiusan 农场。水马厩总数在开垦的起始的阶段严厉地减少了然后与时间逐渐地变得稳定。他们显著地在黑土壤与器官的 C，全部的 N，全部的 P，和 CEC 的内容被相关，与是的相关系数 r 0.76， 0.68， 0.61，和 0.81 (P 【 0.01 ) 分别地；然而，他们与可得到的 P，可得到的 K，和全部的 K 的关系是不清楚的。这些证明那有机物是土壤水马厩总数的硬化。增加的分解和在开垦以后的有机物的减少的输入为水马厩总数的减小的数量负责。因此，维持良土团聚体结构，注意应该对土壤营养素地位的改进被给予，特别器官的 C 和 N 的供应。

Light Fraction Carbon and Water-Stable Aggregates in Black Soils

The distribution of light fraction carbon (LF-C) in the various size classes of aggregates and its relationship to water-stable aggregates as well as the influence of cultivation on the organic components in virgin and cultivated black soils were studied by wet sieving and density separation methods. The total organic carbon (TOC) and LF-C were significantly higher (P≤0.05) in the virgin soils than in the cultivated soils. The LF-C in aggregates of different size classes varied from 0.9 to 2.5 g kg-1 in the cultivated soils and from 2.5 to 7.1 g kg-1 in the virgin soils, whereas the ratio of LF-C to TOC varied from 1.9% to 7.3% and from 5.0% to 12.2%, respectively. After being incubated under constant temperature and controlled humidity for three months, the contribution of LF-C to TOC sharply decreased to an amount (1.7%-8.5%) close to the level in soils that had been cultivated for 20 to 25 years (1.3%-8.8%). As a result, the larger water-stable macro-aggregates (especially > 1 mm) decreased sharply, indicating that the LF-C pool in virgin soils declined quickly after cultivation, which reduced the water stability of soil aggregates.

Effects of Long-Term Winter Planted Green Manure on Distribution and Storage of Organic Carbon and Nitrogen in Water-Stable Aggregates of Reddish Paddy Soil Under a Double-Rice Cropping System

In agricultural systems, maintenance of soil organic matter has long been recognized as a strategy to reduce soil degradation. Manure amendments and green manures are management practices that can increase some nutrient contents and improve soil aggregation. We investigated the effects of 28 yr of winter planted green manure on soil aggregate-size distribution and aggregateassociated carbon（C） and nitrogen（N）. The study was a randomized completed block design with three replicates. The treatments included rice-rice-fallow, rice-rice-rape, rice-rice-Chinese milk vetch and rice-rice-ryegrass. The experiment was established in 1982 on a silty light clayey paddy soil derived from Quaternary red clay（classified as Fe-Accumuli-Stagnic Anthrosols） with continuous early and late rice. In 2009, soil samples were collected（0-15 cm depth） from the field treatment plots and separated into water-stable aggregates of different sizes（i.e., 〉5, 2-5, 1-2, 0.5-1, 0.25-0.5 and 〈0.25 mm） by wet sieving. The long-term winter planted green manure significantly increased total C and N, and the formation of the 2-5-mm water-stable aggregate fraction. Compared with rice-rice-rape, rice-rice-Chinese milk vetch and rice-rice-ryegrass, the rice-rice-fallow significantly reduced 2-5-mm water-stable aggregates, with a significant redistribution of aggregates into micro-aggregates. Long-term winter planted green manure obviously improved C/N ratio and macro-aggregate-associated C and N. The highest contribution to soil fertility was from macro-aggregates of 2-5 mm in most cases.

The Effects of Different Ratios of Feldspathic Sandstone and Sand Compound Soil on Water-stable Aggregates in 4 Years Crop Planting

To study the formation process of feldspathic sandstone and sand compound soil in the Mu Us Desert,1∶ 1,1∶ 2 and 1∶ 5 ratios of feldspathic sandstone and sand were mixed to obtain compound soil to plant crops,and analyze the rules of changes in water-stable aggregates of the compound soil among the 4 years crops growing process. The results showed,before crop planting,the order of mass percent of> 0. 25 mm and 0. 25-2. 00 mm water-stable aggregates in three kinds of compound soil was 1∶ 1 > 1∶ 2 > 1∶ 5,showing that the overall content was low; the mass percent of > 0. 25 mm water-stable aggregates remained at 18. 38%-28. 22%; the mass percent of 0. 25-0. 50 mm,0. 50-2. 00 mm,2. 00-5. 00 mm,and > 5. 00 mm water-stable aggregates was close with each other in each kind of compound soil. After4 years of planting,the mass percent of > 0. 25 mm water-stable aggregates in 1∶ 2 compound soil increased significantly and exceeded other2 kinds of compound soil,reached 32. 34%; the main components of > 0. 25 mm water stable aggregates in 1∶ 1,1∶ 2,and 1∶ 5 compound soil were 0. 25-0. 50 mm( 53. 54%),0. 25-0. 50 mm( 59. 43%),0. 05-2. 00 mm( 52. 16%),aggregates; 0. 25-2. 00 mm aggregates increased significantly in all three kinds of compound soil,with the highest increase in 1∶ 2 compound soil; the organic matters of 1∶ 2 compound soil were significantly correlated with 0. 25-0. 50 mm and 0. 25-2. 00 mm water-stable aggregates. The results showed that the ratio of 0. 25-2. 00 mm aggregates in the three kinds of compound soil was increased after 4 years of crop planting and 1∶ 2 compound soil was most favorable for the formation of aggregates.

Accumulative Effect of Soil Organic Carbon and Nitrogen in Water-Stable Aggregates and Soil Stability Characteristics of Robinia pseudoacacia Plantation in the Loess Hilly-Gully Region

Soil water-stable aggregates (WSAs) are the basic unit of soil constitution and can contribute to remaining the stable soil constitution. The objective of this study was to clarify the distribution and stability of WSAs and the soil organic carbon (SOC), the total nitrogen (TN), and the total phosphorus (TP) concentrations in 0 - 20 cm and 20 - 40 cm soil layers under the different ages of Robinia pseudoacacia plantations. The 20, 25, 40, and 50 years-old Robinia pseudoacacia plantations were selected. Stepwise regression analysis showed that >5 mm and 1 - 2 mm WSAs, SOC concentration in 2 - 5 mm WSAs, and TN and TP concentrations in < 0.25 mm WSAs were dominant independent variables affecting aggregate stability and that SOC in 0.25 - 0.5 mm WSAs, TN in <0.25 mm and 1 - 2 mm WSAs and TP in 2 - 5 mm WSAs were dominant independent variables affecting SOC, TN, and TP concentrations in bulk soils.

Physical and Microbiological Properties of Alfalfa-Established Soil in the Semiarid Horqin Sandy Land in Northern China

In a previous article, we reported that a local variety of alfalfa (Medicago sativa L. cv. Aohan) had high potential to be a pioneer plant for ecological restoration in the Horqin Sandy Land, China. The plantation of Aohan significantly improved the organic matter, clay, total carbon and nitrogen contents of the soils. In this study, we investigated the physical properties such as dispersion ratio, water-stable aggregates content, and the soil microbiomes, five years after alfalfa establishment in the same study site. We found no significant difference in the dispersion ratios between the soils before and after alfalfa establishment, and all the soils at the study site were erosive. Water stable aggregates mainly distributed in 96%, suggesting that it would take longer time for improving soil structure. However, large-size aggregates (2 - 5 mm) content was slightly higher in the alfalfa planting plots. This slight increase is presumed to have long-term importance for soil and ecosystem recovery in semi-arid areas like Horqin Sandy Land. Moreover, we also found that Actinomycetes dominated the microbial community in both bulk and rhizosphere soils, and two kinds of rhizobia, Bradyrhizobium and Sinorhizobium fredii, were identified in the rhizosphere soil.

Role of Organic Matter in Formation and Stability of Aggregates in Mulberry Plantation Soils

The role of organic matter in the formation and stability of soil aggregates in mulberry plantation in the Hang-Jia-Hu Plain, northern Zhejiang Province, was evaluated in this study. A positive correlation was found between water-stable aggregate contents and organic matter contents in the mulberry plantation soils, which supported the hypothesis that organic matter was the main cementing agent in formation of aggregates. A close correlation was also found between stability of aggregate and organic matter contents. Regression analysis showed that total nitrogen content was also an indicator of water-stable aggregate content and stability. The aggregate size distribution indicated that the water-stable aggregates 1--0.25 mm in diameter were the major component of the aggregates in the mulberry plantation soils. The organic matter contents of aggregates ranging from 5 to 0.25 mm in diameter increased with the decrease of aggregate sizes, and the aggregates 1-0.25 mm in diameter had the maximum organic matter content.

Arbuscular Mycorrhizal Fungus Mediate Changes in Mycorrhizosphere Soil Aggregates

Many studies have shown that arbuscular mycorrhizal (AM) fungus has an important role in soil aggregate formation and stabilization. While most studies about the effects of AM fungus on soil aggregate have experimental set-ups in single pots or containers with two compartments, these studies cannot differentiate the effects of roots, mycorrhizal roots or hyphae. In this study we used containers with four compartments to split the roots and quantitatively compare the change of soil aggregate in the mycorrhizosphere soil, rhizosphere soil, hyphosphere soil and bulk soil. Our results demonstrate a significant positive correlation among hyphal length density, easily extractable glomalin (EEG) and aggregate mean weight diameter (MWD), geometric mean diameter (GMD) and percentage of soil macroaggregate with a diameter larger than 0.25 mm (R0.25). The GMD and MWD of R0.25 in the hyphal compartment were higher than those in the non-inoculated root compartment, but were lower than those in the mycorrhizal compartment. This suggests the mycorrhizal hyphae had a greater effect than the non-inoculated roots, but less of an effect than the mycorrhizal roots on the formation and stabilization of soil aggregate. The results reveal that plant roots, mycorrhizal roots and mycorrhizal hyphae contribute to aggregate stability in individual ways and that their effects are additive, creating a synergistic stabilizing effect.

Impact of Integrated Nutrient, Crop Residue and Tillage Management on Soil Aggregates and Organic Matter Fractions in Semiarid Subtropical Soil under Soybean-Wheat Rotation

Various physical, chemical and biological soil properties in surface (0-5 cm) and subsurface (5-15 cm) soil were determined in a 4-year field experiment conducted at Punjab Agricultural University Ludhiana, India with sixteen treatments consisted of different combinations of fertilizer N (0, 20 and 25 kg N ha-1), P (0, 60 and 75 kg P2O5 ha-1), FYM (0 and 10 t&middot;ha-1) and wheat (Triticum aestivum L.) residue (WR) (0 and 6 t&middot;ha-1) applied to summer-grown soybean (Glycine max L.) and fertilizer N (0, 120 and 150 kg N ha-1), P (0, 60 and 75 kg P2O5 ha-1), and soybean residue (SR) (0 and 3 t&middot;ha-1) applied to winter-grown wheat crop continuously in both conventional tillage (CT) and conservation agriculture (CA), arranged in a split-split plot design with tillage system in main blocks, under irrigated subtropical conditions. Application of fertilizer N, P, FYM and crop residue (CR) significantly increased water stable aggregates and had profound effects in increasing the mean weight diameter as well as the formation of macro-aggregates, which were the highest in both surface (85%) and subsurface (81%) soil layers with application of 20 kg N + 60 kg P2O5 + 10 t FYM + 6 t WR ha-1 applied to soybean and 120 kg N + 60 kg P2O5 + 3 t SR ha-1 applied to wheat crop in CA, respectively, and were 83% and 77% in CT treatments after 2 years. Hence, better aggregation was found with 100% NP + FYM + CR, where macro-aggregates were greater than 50% of total soil mass. The same treatment also enhanced total organic C (TOC) from 3.8 g&middot;kg-1 in no-NP-FYM-CR control to 5.8 g&middot;kg-1 in surface layer and from 2.7 to 3.6 g&middot;kg-1 in subsurface layer after 2 years leading to the 41% and 39% higher TOC stocks over CT-Control in 0-15 cm soil layers of CT and CA, respectively. The changes in TOC stocks after 4 years were 52% and 59%.

稻虾共作模式下克氏原螯虾对水稻土团聚体特征及有机碳分布的影响

为研究长期稻虾共作模式下克氏原螯虾对水稻土团聚体数量、稳定性以及土壤团聚体有机碳含量、分布的影响,本研究依托始于2014年的长期定位试验,设置冬泡无秸秆还田(W)、冬泡+秸秆还田(WS)和冬泡+秸秆还田+养虾(WSC)3个处理,采集各处理表层土壤样品,分析土壤团聚体及有机碳分布特征。结果表明:土壤水稳性团聚体以>1~2 mm粒级为主,在冬泡条件下秸秆还田处理显著提高了土壤中>2 mm团聚体比例、有机碳含量和有机碳贡献率,且增加了土壤团聚体平均质量直径;相对于冬泡+秸秆还田处理,冬泡+秸秆还田+养虾处理土壤>2 mm和>1~2 mm团聚体比例显著提高了19.8%和6.5%,而0.053~0.25 mm团聚体比例则显著下降了9.2%,且>0.25 mm的团聚体数量、平均质量直径和几何平均直径分别提高了7.8%、20.6%和37.5%。冬泡+秸秆还田+养虾处理的土壤总有机碳、微生物量碳和颗粒有机碳含量较冬泡+秸秆还田处理分别显著提高了6.2%、34.0%和23.1%,且显著提高了<0.25 mm团聚体中有机碳的含量。研究表明,稻虾共作模式下克氏原螯虾养殖促进了土壤大团聚体的形成,增强了团聚体稳定性,增加了土壤有机碳及活性组分含量,尤其提高了微团聚体中有机碳含量,提升了土壤固碳能力。

餐厨有机肥对设施菜地土壤理化性质的影响

为探讨餐厨有机肥对设施菜地土壤理化性质的影响,使用商品有机肥料为对照,开展了设施菜地餐厨有机肥的田间试验。结果显示,施用餐厨有机肥料可提高蔬菜产量5.3%~11.6%,增产效果和商品有机肥无显著差异。餐厨有机肥可提高设施菜地土壤有机质含量25.5%~31.8%、土壤速效氮含量8.6%~11.3%、土壤有效磷含量3.1%~12.6%、土壤速效钾含量6.5%~8.2%,但餐厨有机肥和商品有机肥处理间土壤有机质、速效氮、有效磷和速效钾含量均未出现显著差异;餐厨有机肥处理的菜地土壤可溶性总盐含量提高4.0%~5.8%,但餐厨有机肥和商品有机肥处理间无显著差异;餐厨有机肥可以提高土壤水稳性大团聚体(R0.25)含量9.9~14.5百分点,餐厨有机肥和商品有机肥处理之间无显著差异。总的来看,餐厨有机肥料和普通商品有机肥的肥效基本类似;餐厨有机肥可提高设施菜地土壤有机质、速效养分和土壤水稳性大团聚体(R0.25)含量,其效果和商品有机肥料基本相似;餐厨有机肥料可导致土壤可溶性总盐含量小幅上升,但上升幅度和商品有机肥料无明显差异。

黑龙滩水库消落带植被恢复模式对土壤水稳性团聚体组成及其稳定性的影响

[目的]为阐明水库消落带不同植被恢复模式对土壤水稳性团聚体组成及其稳定性的影响。[方法]以川中丘陵区黑龙滩水库消落带人工恢复与自然恢复植被下的土壤为研究对象,利用湿筛法测定分析土壤水稳性团聚体组成特征及其稳定性指标。[结果]消落带土壤>0.25 mm水稳性大团聚体含量与未淹水对照相比降低4.21%,其中,自然恢复植被下土壤水稳性大团聚体含量较对照下降12.27%,人工恢复植被下大团聚体含量较对照增加3.84%;不同植被恢复模式下土壤水稳性团聚体组成差异显著(p<0.05),人工恢复植被下土壤大团聚体含量(69.48%)显著高于自然恢复模式(43.20%);消落带土壤水稳性团聚体稳定性随水位高程增加而升高,团聚体稳定性指标>0.25 mm水稳性团聚体含量(R_(0.25))、平均重量直径(MWD)、几何平均直径(GMD)人工恢复模式下均大于自然恢复模式,团聚体分形维数(D)、土壤可蚀性因子(K)与团聚体破坏率(PAD)人工恢复模式小于自然恢复模式,表明人工恢复模式下土壤水稳性团聚体稳定性、土壤抗蚀性高于自然恢复模式。[结论]黑龙滩水库消落带在淹水后土壤团聚体稳定性下降,但人工恢复模式能有效改善土壤团粒结构,研究结果可为黑龙滩水库消落带土壤结构稳定性评价及植被恢复提供科学依据。

黑土物理特性变化及改善途径

东北黑土区位于世界四大黑土区之一,因其高肥力和有机质含量而闻名。作为我国主要的粮食生产基地之一,在保障国家粮食安全方面具有重要地位。然而,随着东北黑土地的开垦,养分的失调导致了黑土物理特性的变化和功能的退化,从而使得黑土肥力下降,威胁着该地区的粮食安全和生态安全。黑土物理特性发生变化,主要体现在黑土层土壤容重增加,孔隙度降低,水稳性团聚体减少,田间持水量下降等。采用合理的耕作方式和施肥制度能在不同程度上改善土壤物理特性,从而遏制土壤肥力的不断下降。本文总结了自黑土开垦以来土壤容重、水稳性团聚体、田间持水量和饱和导水率的变化,分析了耕作和有机物料等对土壤物理特性的重要调节作用,讨论了不同保护途径如何通过改善土壤物理特性达到提升土壤肥力的目的,最后从制定合理的耕作体系和有机培肥机制,优化作物种植模式等展望了未来黑土重点研究的方向。

川中丘陵区紫色土坡耕地植物篱措施对土壤抗蚀性的影响

川中丘陵区紫色土坡耕地水土流失严重,影响农作物增产增收。水土保持植物篱措施是提高土壤的抗侵蚀能力的有效生态手段。以往土壤抗蚀性的研究多集中于黄土高原,较少涉及植物篱作用下紫色土坡耕地抗蚀机理方面的研究。本文以新银合欢(Leucaena leucocephala)和香根草(Vetiveria zizanioides)为研究对象,基于试验站坡耕地小区定位观测的基础数据,结合土壤采样和实验分析,利用主成分分析法对种植不同植物篱的坡耕地土壤抗蚀性进行了综合评价。结果表明:(1)在分析11项指标后,2项主成分指标对解释土壤抗蚀性贡献率达到82.7%,其中水稳性团聚体被认定为关键指标。(2)植物篱显著提高了表层土的抗蚀性。与无植物篱的样区为对照,10°坡耕地上香根草和新银合欢植物篱小区的土壤抗蚀性综合指数(SERI)分别比对照区高2.048和0.853,15°坡耕地上香根草植物篱的SERI比对照区高1.321;且植物篱下侧的土壤抗蚀性高于植物篱上侧,在10°和15°香根草植物篱小区,植物篱下侧抗蚀性指数分别达到上侧的2.0倍、1.5倍,而10°新银合欢植物篱小区的下侧抗蚀性综合指数达到上侧的1.1倍。(3)相较于新银合欢植物篱小区,香根草植物篱小区土壤表现出了更高的抗蚀性,且两种植物篱小区下侧土壤抗蚀性均优于上侧,选择密植草本植物篱并重点防治植物篱上侧土壤可有效减少坡耕地水土流失。研究成果可为川中丘陵区水土流失治理及农业增产提供科学依据。

不同土地利用方式砖红壤团聚体水稳性及其对前期含水率的响应

前期含水量是影响土壤团聚体稳定性的重要因素,而其对砖红壤结构稳定性的影响缺乏系统研究,本文以海南地区3种典型利用方式(林地、荒地、耕地)下玄武岩发育砖红壤团聚体为研究对象,测定其理化性质,干、湿筛团聚体组成及不同前期含水率(3%、5%、10%、15%、20%)条件下3~5 mm粒径团聚体破碎后粒径分布状况,采用冗余分析探究了土壤性质及前期含水率对不同土地利用方式砖红壤团聚体水稳性的影响。结果表明:(1)不同土地利用方式下,土壤部分性质存在显著差异,如土壤pH、有机碳、阳离子交换量及部分交换性盐基离子等,其余性质差异较小。(2)土壤水稳性团聚体组成总体呈现“单峰”或“双峰”分布,峰值主要出现在2~1 mm和0.5~0.25 mm处,对于表层土壤而言,林地土壤团聚体稳定性最高,而耕地土壤团聚体稳定性较弱,表下层土壤稳定性显著(P<0.05)低于表层土壤。(3)在风干条件下,表层土壤团聚体水稳性普遍较高(WSA>90%,MWD>1.5,GMD>1.2),随着前期含水率的增加,团聚体破碎后大团聚体(>2 mm)含量有不同程度的变化,整体呈现水稳性大团聚体含量随前期含水率增加而增加。(4)土壤有机碳是影响砖红壤团聚体水稳性的最主要因素,其对团聚体水稳性差异的解释率达80.6%。

不同种植年限苜蓿地球囊霉素相关土壤蛋白含量及其影响因素

球囊霉素相关土壤蛋白(GRSP)是丛枝菌根真菌(AMF)分泌的一种糖蛋白,有利于土壤团聚体形成。本研究借助于黄土高原半干旱区的长期定位试验,以不同建植年限(L2019、L2012、L2003)紫花苜蓿(Medicago sativa)草地为研究对象,玉米(Zea mays)田为对照,研究GRSP含量的主要调控因子及其与土壤有机碳和团聚体结构特征的关系。结果表明,随苜蓿种植年限增加,AMF丰度(侵染率、菌丝密度)和GRSP含量显著提高(P<0.05),线性回归分析结果表明,总提取球囊霉素相关土壤蛋白(T-GRSP)含量与土壤有机碳和微生物生物量碳之间显著正相关(P<0.05),而易提取球囊霉素相关土壤蛋白(EE-GRSP)和总提取球囊霉素土壤蛋白含量均与水稳性团粒特征平均重量直径(MWD)和大于0.25 mm的团聚体含量(R0.25)之间显著正相关(P<0.05),冗余分析(RDA)结果表明,影响土壤AMF丰度和GRSP含量的主要环境因子是土壤速效磷(P=0.002)和微生物量碳(P=0.002)。与农田相比,T-GRSP含量在土壤有机碳中的比例随苜蓿种植年限的增加而提高,说明持续多年种植苜蓿引起GRSP含量增加,促进GRSP含量在土壤有机碳中的积累,提高了GRSP含量对土壤团聚体稳定性和有机碳的贡献。该研究结果可为黄绵土GRSP研究及紫花苜蓿栽培草地可持续利用提供理论依据。