Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Spatial differences of Sustainable Development Goals(SDGs)among counties(cities)on the northern slope of the Kunlun Mountains

The county(city)located on the northern slope of the Kunlun Mountains is the primary area to solidify and extend the success of Xinjiang Uygur Autonomous Region,China in poverty alleviation.Its Sustainable Development Goals(SDGs)are intertwined with the concerted economic and social development of Xinjiang and the objective of achieving shared prosperity within the region.This study established a sustainable development evaluation framework by selecting 15 SDGs and 20 secondary indicators from the United Nations’SDGs.The aim of this study is to quantitatively assess the progress of SDGs at the county(city)level on the northern slope of the Kunlun Mountains.The results indicate that there are substantial variations in the scores of SDGs among the nine counties and one city located on the northern slope of the Kunlun Mountains.Notable high scores of SDGs are observed in the central and eastern regions,whereas lower scores are prevalent in the western areas.The scores of SDGs,in descending order,are as follows:62.22 for Minfeng County,54.22 for Hotan City,50.21 for Qiemo County,42.54 for Moyu County,41.56 for Ruoqiang County,41.39 for Qira County,39.86 for Lop County,38.25 for Yutian County,38.10 for Pishan County,and 36.87 for Hotan County.The performances of SDGs reveal that Hotan City,Lop County,Minfeng County,and Ruoqiang County have significant sustainable development capacity because they have three or more SDGs ranked as green color.However,Hotan County,Moyu County,Qira County,and Yutian County show the poorest performance,as they lack SDGs with green color.It is important to establish and enhance mechanisms that can ensure sustained income growth among poverty alleviation beneficiaries,sustained improvement in the capacity of rural governance,and the gradual improvement of social security system.These measures will facilitate the effective implementation of SDGs.Finally,this study offers a valuable support for governmental authorities and relevant departments in their decision-making processes.In addition,these results hold significant reference value for assessing SDGs at the county(city)level,particularly in areas characterized by low levels of economic development.

Economy Function in the Mode of Sustainable Development

To implement the previously formulated principles of sustainable economic development, all solutions of the linear system of equations and inequalities, which are satisfied by the vector of real consumption, are completely described. It is established that the vector of real consumption with the minimum level of excess supply is determined by the solution of some quadratic programming problem. The necessary and sufficient conditions are established under which the economic system, described by the “input-output” production model, functions in the mode of sustainable development. A complete description of the equilibrium states for which markets are partially cleared in the economy model of production “input-output” is given, on the basis that all solutions of system of linear equations and inequalities are completely described. The existence of a family of taxation vectors in the “input-output” model of production, under which the economic system is able to function in the mode of sustainable development, is proved. Restrictions were found for the vector of taxation in the economic system, under which the economic system is able to function in the mode of sustainable development.

Pathway and Countermeasure of Farmers' Livelihood Transformation in the Ecological Development Area of Northern Guangdong Province from the Perspective of Ecological Protection

[Objectives]To explore pathways and countermeasures for transforming farmers livelihoods in the way of reducing their dependence on land while promoting sustainable development and alleviating ecological degradation.[Methods]A combination of field research,literature review,and policy analysis was employed to identify key factors affecting farmers livelihoods and potential strategies for transformation.[Results]The study found that developing ecological agriculture and modern agriculture,promoting agricultural transformation and upgrading,cultivating alternative industries,strengthening ecological engineering construction,and establishing diversified ecological compensation methods and supporting policies are effective strategies for transforming farmers livelihoods.[Conclusions]Implementing these strategies can help alleviate the contradiction between ecological protection and farmers livelihood development,promoting coordinated development of both.This approach not only benefits farmers but also contributes to sustainable environmental management and biodiversity conservation.

Professional Development of Rural PE Teachers from the Perspective of PCK:A Case Study of Huaiji County

Based on the four dimensions of PCK,this paper investigates the professional development of rural PE teachers in Huaiji County by using the research methods of literature,questionnaire,interview and mathematical statistics.Based on PCK related theories,this paper analyzes the current situation of professional development of rural PE teachers in Huaiji County,explores the problems existing in the process of professional development,and provides an optimal path for the professional development of rural PE teachers,so as to improve the teaching ability and classroom quality of rural PE teachers,and promote the further development of rural PE education under the background of new curriculum reform.Through the research,it is found that the shortcomings of rural PE teachers are:lack of situational knowledge;lack of understanding of the course content;not flexible use of teaching representation;low ability to identify the differences of students learning styles;lack of understanding of social environment,etc.

Research on the Impact and Opportunities of the Digital Economy Promoting the Development of Real Economy

The digital economy has infused vitality into the transformation and development of the real economy,urging enterprises to break through core technological barriers,address bottleneck issues,and improve their core competitiveness.It fosters the comprehensive digital transformation of agriculture,manufacturing,and service sectors,improving the dynamism of the real economy and fostering more consumption hotspots.Efforts are underway to enhance industrial supply chains and innovation chains,optimize regional resource allocation,and promote a virtuous cycle within the real economy.Initiatives are being undertaken to standardize the development of digital economic platforms,promote the high-quality development of regional economies,and leverage the advantages of the socialist market economy with Chinese characteristics.

Integrated Development of Tea and Tourism in Taishan Mountain Tea Valley in the Context of Rural Revitalization

In recent years,the integrated development of the tea and tourism has become an important way to promote the rural revitalization of Tai'an tea-producing areas,but the state of industrial upgrading still needs to be improved.In order to better integrate tea and tourism in Tea Valley,this paper uses questionnaire and SWOT analysis to study.It found the following problems:(i)45.7%of tourists were interested in tea culture,but they know litle about it,and the integration of tea culture and tea tourism products is low.(i)50.54%of the tourists were interested in the tea picking experience project and wanted to add the tea food experience item.(ii)The acceptance of 45.7%of tourists to scenic spots was 1-2 h,which shows that the quality of transportation service facilities and personnel needs to be improved.Combined with the SWOT analysis,it is found that the scenic spot is rich in resources,convenient transportation,profound cultural heritage,and good govern-ment policy support conditions,which is suitable for the SO strategy(growth development strategy),so the scenic spot relies on internal ad-vantages and seizes external opportunities to develop the integration of tea and tourism.Based on the analysis results,it came up with the perti-nent recommendations for the key problems:(i)The infrastructure service is not well established.It is suggested that scenic spots should in-crease investment,regularly train service personnel to improve their quality,and improve transportation services,accommodation,catering and public service hardware facilities.(i)Tea tourism products are insufficient.It is suggested that tea tourism products should be innovatively designed and tea tourism experiential products and tourism commodities should be planned.(i)Tea culture and tourism lack organic combi-nation.It is recommended to deeply excavate tea culture,and take consideration of both spiritual and material aspects.The specific methods include building teahouses,Taishan Mountain(Mount Tai)tea culture throughout the whole process of tea production,development and pro-cessing,and AR tea culture experience hall can be built to make tourists feel tea culture from dfferent perspectives.(iv)The publicity is in-sufficient.Strategies should be made in three aspects:improving the Internet media,taking into account the traditional media,and expanding the market based on the surrounding areas.(v)The tea industry chain is short.It is recommended to add four new industrial chain designs of"tea+accommodation,tea+catering,tea+Intemet,tea+training",hoping to bring some inspiration and ideas to the managers of scenic spots.

The Role and Place of Artificial Neural Network Architectures Structural Redundancy in the Input Data Prototypes and Generalization Development

Neural Networks (NN) are the functional unit of Deep Learning and are known to mimic the behavior of the human brain to solve complex data-driven problems. Whenever we train our own neural networks, we need to take care of something called the generalization of the neural network. The performance of Artificial Neural Networks (ANN) mostly depends upon its generalization capability. In this paper, we propose an innovative approach to enhance the generalization capability of artificial neural networks (ANN) using structural redundancy. A novel perspective on handling input data prototypes and their impact on the development of generalization, which could improve to ANN architectures accuracy and reliability is described.

Spatial Protection and Development of Traditional Mountain Settlements from the Perspective of Self-organization : A Case Study of Anju Ancient Town in Tongliang District, Chongqing

Through literature research, field research and urban design analysis methods, based on the self-organization theory, the evolution characteristics of the settlement space of Anju Ancient Town with significant characteristics of traditional mountain settlements were analyzed, and then its internal and external influencing factors were summarized. Finally, appropriate protection and development strategies were proposed, such as reasonably planning functional zones, breaking through the single structure, and creating a good living cultural environment. The innovation of this study is that it integrates the self-organization theory into the cognition of traditional mountain settlement space, and discusses the connection between the mountain settlement space and the traditional regional context under the modern development concept, so as to seek the benign protection and reasonable development of traditional mountain settlement space.

Research on the Development Path and Mechanism of“Double-Qualified”Teachers in Private Universities Under the Background of Digital Transformation

This article explores the development path and mechanism of“double-qualified”teachers in private universities under digital transformation.Through literature review,theoretical analysis,and other research methods,this study conducts an in-depth analysis of the impact of digital technology on the development of“double-qualified”teachers in private colleges and universities and puts forward corresponding strategies and suggestions.Research shows that digital transformation provides new opportunities and challenges for developing“double-qualified”teachers in private colleges and universities.To adapt to this trend,teachers must continuously improve their digital skills,and schools should establish a complete incentive mechanism and evaluation system,strengthen school-enterprise cooperation and the integration of industry and education to promote the comprehensive development of“double-qualified”teachers,and provide a useful guideline for private universities to promote the development of“double-qualified”teachers in digital transformation.

Exploration of the Curriculum Reform Path in the Period of High-Quality Development of University Educational Psychology

The theoretical and applied characteristics of educational psychology courses in colleges and universities are prominent,and actively promoting curriculum reform is more conducive to promoting the implementation of talent training goals,which is in line with the practical needs of social development for high-quality talents.However,curriculum reform is a systematic project,which involves the innovation of curriculum content systems,teaching methods,teaching evaluations,and so on.This paper mainly expounds on the curriculum reform path in the period of high-quality development of educational psychology in colleges and universities,hoping to play a reference role in improving the level of education management.

Research on the Development Strategy of Smart Tourism in Hainan Under the Background of Big Data

Hainan is a major tourist province.It is urgent to promote the transformation and upgrading of Hainan’s tourism industry from a traditional service industry to a modern service industry by means of informatization.Smart tourism is a brand-new tourism form and operation mode of tourism transformation and upgrading.Integrating big data technology will make smart tourism more accurate in three aspects:tourism management,tourism service,and tourism marketing,and further enhance the satisfaction of the tourism experience.This paper studies the development status of smart tourism in Hainan,deeply summarizes its existing problems and causes,and puts forward the development strategy of smart tourism in Hainan to promote the healthy development of the tourism industry in Hainan.

The Impact of Human Resource Management on University Development

As competition in the education industry intensifies and the knowledge economy evolves,the significance of Human Resource Management(HRM)in university institutions.This study aims to explore how HRM affects the sustainable development and competitiveness improvement of universities.This article begins with a theoretical analysis to define the concept of HRM and its particular relevance within university education.The subsequent analysis examines the multi-dimensional framework of university development,encompassing its connotation,goals,and key influencing factors.The article further elaborates on the positive effects of HRM on university teaching quality,scientific research capabilities,organizational culture,and social services.On this basis,the main challenges currently faced by university HRM are discussed,such as talent mobility,institutional constraints,resource limitations,and internationalization pressure.Finally,optimization strategies are proposed,including building a scientific human resources system,enhancing talent training and development,fostering diversity among teaching staff,and improving decision-making efficiency and transparency.The conclusions of this study aim to provide strategic insights for university education managers to better utilize human resource advantages and promote the comprehensive and sustainable development of universities.

Comparison of the Development Model of DTP Pharmacy between China and the United States and Its Enlightenment

Objective To systematically analyze the development status of DTP pharmacy in the United States and China,and to find out the problems in China’s DTP pharmacy and put forward some countermeasures and suggestions.Methods Relevant literature was searched and reviewed to study the history,market scale and development trend of DTP pharmacy in the United States and China.Then,the problems in China’s DTP pharmacy were found out.Results and Conclusion DTP pharmacy model in the U.S.has taken shape and is maturing.The high degree of industry concentration is an important feature of the U.S.pharmacy.In contrast,China’s DTP pharmacy started late,and the industry pattern has not yet been finalized.There are still some problems in the DTP pharmacy certification and quality management.It is recommended to promote the rapid development of DTP pharmacies in China by encouraging the outflow of prescription drugs,establishing a third-party pharmacy accreditation organization and strengthening the team of licensed pharmacists and other personnel.

Exploration of the Evolution of Teacher Roles and Professional Development Needs Among University English Instructors

The role of university English instructors has evolved significantly over the years,encompassing various aspects.This paper explores the evolution of the role of university English instructors and examines the current challenges and opportunities they face in today’s educational landscape.It delves into the multifaceted nature of their responsibilities and discusses the importance of continuous professional development to meet the evolving needs of students and the demands of the profession.

The Market Development of Children’s Go Training and the Difficulty and Countermeasure Analysis of Go Training

The children’s Go training market is experiencing vigorous growth but also faces several challenges.As the importance of Go in cultural education increases,the market expands,with growing demand and involvement from training and educational institutions.However,the market encounters difficulties such as a shortage of high-quality Go coaches,uniformity in training content and methods,and high training costs.To address these issues,measures can be taken to strengthen the team of Go coaches,diversify training content and methods,and optimize the training cost structure,thereby promoting the healthy development of the children’s Go training market.

Reform and Development of English Teaching in Colleges and Universities with the Integration of ChatGPT Technology

As an artificial intelligence-powered language generation model based on natural language processing,ChatGPT,like other artificial intelligence technologies,possesses super real-time dialogue capabilities and is considered an innovative and intelligent auxiliary teaching tool in the field of language learning.This article first analyzes the current state of development of artificial intelligence technologies such as ChatGPT.Secondly,it examines the challenges encountered in the reform and development of English teaching in colleges and universities in China.Lastly,it summarizes the applications and recommendations for using ChatGPT technology in college-level English teaching.

Analysis of Innovative Paths for Enterprise Marketing Management Development Strategy under the Background of New Media

From the perspective of enterprise marketing strategy planning,innovative research should be conducted based on the current trends in new media.The findings should be applied to practical activities within enterprises to achieve innovation in marketing strategy decision-making in the new media environment.Enterprises should adopt advanced communication transmission technologies and big data processing capabilities to effectively leverage the hidden value and promotional planning advantages of network media platforms.This approach can enhance customer loyalty and increase demand for new products.Enterprises should integrate into the continuously evolving new media landscape,explore their potential business characteristics,and innovate promotional methods.By effectively combining product information with digital media content,companies can ultimately achieve significant increases in corporate interests.

Research on the Role of Guiding Students to Participate in Skills Competitions on the Development of Young Teachers in Vocational Colleges

This paper explores the role of guiding students to participate in skills competitions on the development of young teachers in vocational colleges.We investigate the impact of skills competitions on the consolidation of professional knowledge,development of innovation capabilities,improvement of hands-on abilities,and enhancement of communication skills with students.The results of this research demonstrate that skills competitions have a positive influence on the professional growth of young teachers.The consolidation of professional knowledge,development of innovation capabilities,improvement of hands-on abilities,and enhancement of communication skills collectively contribute to the effectiveness of young teachers in vocational colleges.It is recommended that vocational colleges prioritize and support the participation of young teachers in skills competitions to foster their professional development.