First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.

Stand establishment,root development and yield of winter wheat as affected by tillage and straw mulch in the water deficit hilly region of southwestern China

Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat （Triti-cum aestivumL.）. At present, little is known about the effect of tilage and straw mulch on the root system of wheat under dryland areas in southwestern China. The aim of this study was to evaluate the effect of three tilage treatments （no-til, NT; rotary til, RT; conventional til, CT） and two crop residue management practices （straw mulch, ML; non-straw mulch, NML） on stand establishment, root growth and grain yield of wheat. NT resulted in lower soil cover thickness for the wheat seed, higher number of uncovered seeds, lower percentage of seedling-less ridges and lower tiler density compared to RT and CT; ML resulted in higher tiler density compared to NML. Straw mulching resulted in more soil water content and root length density （RLD） at most of the growth stages and soil depths. The maximum RLD, root surface area density and root dry matter density were obtained under NT. In the topmost 10 cm soil layer, higher RLD values were found under NT than those under RT and CT. There were no signiifcant differences in the yield or yield components of wheat among the tilage treatments in 2011–2012, but NT resulted in a signiifcant higher yield compared to RT and CT in 2012–2013. Grain yield was signiifcantly higher in ML compared to in NML. A strong relationship was observed between the water-use efifciency and the grain yield. Both NT and ML proved beneifcial for wheat in term of maintaining higher tiler density, better soil water status and root growth, leading to a higher grain yield and enhanced water-use efifciency, especialy in a low rainfal year.

Establishment and assessment of rodent models of medication-related osteonecrosis of the jaw(MRONJ)

Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are still not fully understood.Animal models can be used to simulate the pathophysiological features of MRONJ,serving as standardized in vivo experimental platforms to explore the pathogenesis and therapies of MRONJ.Rodent models exhibit excellent effectiveness and high reproducibility in mimicking human MRONJ,but classical methods cannot achieve a complete replica of the pathogenesis of MRONJ.Modified rodent models have been reported with improvements for better mimicking of MRONJ onset in clinic.This review summarizes representative classical and modified rodent models of MRONJ created through various combinations of systemic drug induction and local stimulation and discusses their effectiveness and efficiency.Currently,there is a lack of a unified assessment system for MRONJ models,which hinders a standard definition of MRONJ-like lesions in rodents.Therefore,this review comprehensively summarizes assessment systems based on published peer-review articles,including new approaches in gross observation,histological assessments,radiographic assessments,and serological assessments.This review can serve as a reference for model establishment and evaluation in future preclinical studies on MRONJ.

Establishment of the GPS Monitoring Network in North China, the Relation of Horizontal Crustal Movement to Stress Field and Seismicity

In the paper, the establishment, measurement, data-processing program and monitoring accuracy of the GPS seismic monitoring network in North China, especially in the Capital-Circle area, have been presented briefly. The relation of horizontal crustal deformation to tectonic movement, stress-field variation and seismicity has been analyzed in detail. The results indicate that the accuracy of GPS measurement has reached the order of 10-9 and the annual rate of horizontal crustal deformation in North China is about 4 ~5 mm. Horizontal crustal movement is a direct indication of the regional stress field. Therefore, by monitoring the time-sequence variation of horizontal crustal motion, it would be possible to investigate the change in the stress field, to analyze the tendency of seismicity and to determine the seismogenic zones.

Establishment of Field Sites to Study Frost Heaving and Related Landforms in Southern Yakutia,Russia

Frost heaving processes have been a focus of study since the late 19th century.Factors controlling frost heave development were investigated over several decades.The mechanisms involved were identified based on numerous field and experimental evidences. However,despite this long history of frost

Establishment and evaluation of a theater influenza monitoring platform

Background:Influenza is an acute respiratory infectious disease with a high incidence rate in the Chinese army,which directly disturbs military training and affects soldiers’health.Influenza surveillance systems are widely used around the world and play an important role in influenza epidemic prevention and control.Methods:As a theater centers for disease prevention and control,we established an influenza monitoring platform(IMP)in 2014 to strengthen the monitoring of influenza-like illness and influenza virus infection.In this study,we introduced the constitution,influenza virus detection,and quality control for an IMP.The monitoring effect was also evaluated by comparing the monitoring data with data from national influenza surveillance systems.The experiences and problems associated with the platform also were summarized.Results:A theater IMP was established based on 3 levels of medical units,including monitoring sites,testing laboratories and a checking laboratory.A series of measures were taken to guarantee the quality of monitoring,such as technical training,a unified process,sufficient supervision and timely communication.The platform has run smoothly for 3 monitoring years to date.In the 2014–2015 and 2016–2017 monitoring years,sample amount coincided with that obtained from the National Influenza Surveillance program.In the 2015–2016 monitoring year,due to the strict prevention and control measures,an influenza epidemic peak was avoided in monitoring units,and the monitoring data did not coincide with that of the National Influenza Surveillance program.Several problems,including insufficient attention,unreasonable administrative intervention or subordination relationships,and the necessity of detection in monitoring sites were still observed.Conclusion:A theater IMP was established rationally and played a deserved role in the prevention and control of influenza.However,several problems remain to be solved.

Prevalence of intestinal parasitic infections in certified food-handlers working in food establishments in the City of Nairobi,Kenya

Most intestinal parasites are cosmopolitan with the highest prevalence in the tropics and subtopics. Rural-to-urban migration rapidly increases the number of food eating places in towns and their environs. Some of these eating estabishments have poor sanitation and are overcrowded, facilitating disease transmission, especially through food-handling. Our investigations in Nairobi, therefore, were set to determine the presence of intestinal parasites in food-handlers with valid medical certificates. Direct and concentrated stool processing techniques were used. Chisquare test and ANOVA were used for data analysis. The parasites Ascaris lumbricoides, Entamoeba histolytica and Giardia lamblia were observed in certified food-handlers. Significant difference was found in parasite frequency by eating classes and gender （χ^2 = 9.49, P = 0.73）, （F = 1.495, P = 0.297）, but not in parasite occurrence between age brackets （χ^2 = 6.99, P = 0.039）. The six-month medical certificate validity period may contribute significantly to the presence of intestinal parasites in certified food-handlers.

Review of Marxist Political Party Theory Innovation Over the 70 Years Since the Establishment of PRC

since the establishment of the People’s Republic of China,the central leader-groups from generation to generation have been continuously exploring and innovating Marxist political party theory in terms of its value target,ideological construction,working style,and organization developments.This process can be categorized into five stages:the founding stage in Mao Zedong’s era,the development stage in Deng Xiaoping’s ear,the promotion stage in Jiang Zemin’s times,the deepening stage under Hu Jintao’s leadership,and the stage of further innovation by Xi Jinping in the new era,with the characteristics of inheritance,development as well as scientificity and valuation.

The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months

Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity.In this study,we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S r RNA gene over the same period in lambs.The results showed that lambs’growth performance,the serum parameters,fecal bacterial community and fecal bacterial functions were all affected(P<0.05)by age of the lambs.Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply(P<0.05)after weaning at 60 days.The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age,increased(P<0.05)sharply after 30 days of age.Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators,including negative(P<0.05)correlations between the relative abundance of Lachnospiraceae UCG-010,Eubacterium coprostanoligenes group,Ruminococcaceae UCG-005,Ruminococcaceae UCG-009,Ruminococcaceae UCG-013,Ruminiclostridium 6,Ruminococcaceae UCG-008,and Oscillibacter with serum concentrations of lipopolysaccharide(LPS),D-lactate dehydrogenase(DLA),immunoglobulin(IgA,IgM,and IgG),and cytokines(interleukin-1β(IL-1β),IL-6,IL-12,and IL-17),tumor necrosis factor-α(TNF-α),and the relative abundance of these genera increased from 45 days of age.In conclusion,these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs,and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.

Factors Impacting Corn (<i>Zea mays</i>L.) Establishment and the Role of Uniform Establishment on Yield

Information from actual farm fields can help corn producers understand the value and importance of establishing uniform crop emergence and within-row plant spacing. Thirty-eight fields planted with corn (Zea mays L.) by North Dakota producers were evaluated to determine the effects of uneven plant emergence timing and within-row plant space variability, as well as identifying contributing factors. Rows within a planter’s width with the most variability yielded 6% less than the least variable rows. Individual ear weights decreased as the number of days after normal emergence (date when 50% of plant stand emerged) increased. Ears next to within-row gaps (>30.5 cm) weighed 11% more than the normally spaced plants. Combined ears from both plants situated <5.1 cm apart weighed 36% more than from a single ear from normally spaced plants. Surface residue and planting speed impacted stand establishment variability more often than other factors measured. Producers should assess each field environment individually in order to identify best practices to achieve uniform stand establishment.

Models, the Establishment, and the Real World: Why Do So Many Flood Problems Remain in the UK?

In spite of the proliferation of research and Government Reports on floods in the UK, there are about 5 million properties located in flood prone areas. Many of the houses have been built during the past 50 years with little regard to the existing and future flood risk. Decision makers, who are part of the Establishment, rely on the outputs of models which are produced by so called “experts”. But this information has been found to be flawed. However, the Establishment is largely unwilling to allow local knowledge when assessing flood risk, even though prevailing methodology suggests otherwise. This situation has grown worse since the formation of the Environment Agency in 1996. The public engagement with flood risk science and its application needs to proceed along a co-production of knowledge model. Three examples of the difficulties of making realistic assessments of fluvial flood risk are described. The problem of surface water flooding is also considered since it too has local causes which may not be accounted for in standard methodology. Finally, more open discussion and co-operation in identifying flood problems in the UK is called for.

Effects of micro-environmental conditions and forest disturbance on the establishment of two Andean palms in Ecuador

The wax palm (Ceroxylon echinulatum) and palm heart (Prestoea acuminata) are key elements in Andean natural forests. This ecosystem is threatened by deforestation and habitat degradation, thus, it is vital to explore the impact of anthropogenic activities on the ecological structure and preferences of these species in order to develop proper conservation strategies. Fifty-seven 400 m2 plots were established in two forest areas, a disturbed forest (n = 30 plots) and an undisturbed forest (n = 27 plots) in the Ecuadorean Andes. Nine micro-environmental variables and the number of individuals of C. echinulatum and P. acuminata in five size classes were recorded in each plot. Logistic regression models helped identify environmental variables that influence the establishment of the species. Results showed that different micro-environmental variables determined the presence of different size classes. Both species were benefitted by the environmental conditions of the disturbed forest. Light availability was important for the establishment of both species, especially for the canopy species (C. echinulatum). This palm was negatively affected by the variable “fallen wood”, while P. acuminata was negatively affected mainly by the steepness of the terrain. The environmental variables for the successful establishment of the studied species that were identified by this study provide clues for the sustainable management of C. echinulatum and P. acuminata in Andean forests.

Establishment and development of Ecological Economics in China over the past three decades

The establishment of China's Ecological Economics was proposed by the famous economist Prof. XU Dixin in 1980. Over the past 34 years since its establishment, Ecological Economics has developed into an interdisciplinary subject combined with natural sciences and social sciences. Its emergence met the demand of human society for entering the Ecological Epoch. The past three decades has witnessed the foundation of eco-economic theories and the emergence of various guiding thoughts and policy suggestions which have made great contributions to China's modernization and reform. The guiding ideology of "coordination between ecology and economy" has been widely acknowledged by governments and people, and the concept of "ecological economy" has enjoyed popular support among the general public, which both play increasingly important roles in guiding practice and have major infl uences around the world.

Dating of the topmost terrace in the Jingxian Basin,Anhui Province:an indication of the establishment of the Qingyijiang River

On the northern fringe of Mt. Huangshan, many river terraces are developed at the junction between mountains and plains. The river terraces are most typical in the Jingxian Basin, Anhui Province, where the Qingyijiang River, one of the longest tributaries of the lower Yangtze River, developed three staircase terraces. The topmost terrace(i.e., the T3 terrace) tread is 38-39 m above river level, and the top of the gravels is generally covered by 3-10.9 m of red clay deposits. Systematic magnetostratigraphy and electron spin resonance dating are used to research the timing of the establishment of the Qingyijiang River. The main results show that the topmost terrace developed no later than 900 kyr ago and that the appearance of the Qingyijiang River occurred correspondingly at least before 900 kyr ago on the northern fringe of Mt. Huangshan. In view of the almost synchronous age,the establishment of the Qingyijiang River was seemingly a response to the Mid-Pleistocene Revolution when the 100 kyr cycle commenced, and the Kunlun-Huanghe movement during the earlymiddle Pleistocene transition. In addition, the establishment of the Qingyijiang River possibly reflected the birth of the modern Yangtze River to some degree. Therefore the timing of the formation of the Yangtze River is restricted to no later than 900 kyr ago based on the appearance of the Qingyijiang

The Tenth Anniversary of Establishment of China National Nonferrous Metals Industries Corporation(CNNMIC)

On the 3 April 1993,in the Great Hall of the People a ceremony of the tenth anniversarywas held by CNNMIC.The Vice-premier Li Lanqing and old leaders Fang Yi,Wang Heshouet al attended this ceremony.Lu Dong,Yuan Baohua,Gao Yangwen and Chen Jinhua et alministerial representatives are present also.The ambassadors and/or envoys from Romania,

5-30 Establishment of Interface Communication between Terminal System and the Treatment Planning System

Treatment of terminal system is mainly through the interface communication protocol for treatment planningsystem to achieve the control of various kinds of medical equipments, including primary collimator, range shifter,scatterer, ridge type filter, multi-leaf collimator, etc. TPS was based on the radioactive source and the patientmodeling to simulate the plan implementation of radiotherapy. The basic structure of block diagram is shown inFig. 1.

The Establishment Meeting of International Society of Bionic Engineering

The establishment meeting of International Society of Bionic Engineering (ISBE) convened grandly at Zhuhai,China in September 14-16, 2010. Related officials and representatives attended the opening ceremony, who camefrom the Research Councils, UK, the National Natural Science Foundation of America, the Bionic Society of Ger-

Feeding Ecology and Establishment of the Naturally-Colonized Freshwater Cichlid, Sarotherodon galilaeus (Pisces: Actinopterigii: Perciformes) from a Man-Made Lake, South-Benin, West Africa

The freshwater cichlid, Sarotherodon galilaeus (Linné, 1758) is an economically and commercially important fisheries species that has naturally colonized and dominated a sand-dragged man-made freshwater lake of Ahozon (South-Benin) where the species made about 85.21% of the fish community. We investigated the feeding ecology of S. galilaeus in order to evaluate resource exploitation and the establishment of this species in Lake Ahozon. During wet, flood and dry seasons, 1189 individuals of S. galilaeus have been monthly sampled in the open water and in the aquatic vegetation habitats. The study showed that S. galilaeus consumed about seventy (70) food items dominated by algae (52.88%) composed of 28 families and 52 genera from blue-green algae, green algae, desmids, and diatoms, sand particles (23.95%), detritus (12.27%) and protozoans (7.68%). Minor preys were rotifers, copepods, cladodera, crustacea and insect parts. Foods items with higher diet occurrence were sand particles with a percentage occurrence of 72.33%, detritus (69.47%), and some algae such as Scenedesmus (58.96%), Closterium (55.68%), and Microcystis (51.30%). Significant (P ≤ 0.05) ontogenetic variations of empty stomachs were recorded. S. galilaeus exhibited a wide diet breadth (DB) ranging between 5.55 and 7.29 that tended to increase with fish sizes. Diet overlaps (&Oslash;jk) varied from 0.77 to 0.97, indicating high diet similarities between different life stage categories. The slope b = 0.560 obtained from Log (body weight)-Log (gut length) linear regression, and the mean ratio of GL/SL = 6.72 ± 2.23 support our findings that S. galilaeus exhibited detritivorous, herbivorous and omnivorous food habits. The broad spectrum of food resources consumed/utilized and the high diet breadth recorded leading to allometric growth, the active breeding and the high propagation of the species, were some indicators of the successful establishment of S. galilaeus in the man-made lake of Ahozon. Sustainable fisheries/ aquaculture exploitation of S. galilaeus and successful valorization of the man-made lake of Ahozon require an integrated management of the lake including the prevention of domestic waste dumpings, the protection of the foraging and spawning grounds, the fertilization of the lake, the introduction of predators to control the population of S. galilaeus , the protection of lake margin to avoid erosion, a planned harvest of the fish stock and a periodic ecological monitoring of Lake Ahozon.