Synthesis and Electronic Structure of A2B Type Halogen Atoms Substituted H3-Triarylcorroles

Seven electron-deficient A_2 B type H_3-triarylcorroles have been synthesized and characterized. The solvent dependence of the electronic absorption and magnetic circular dichroism（MCD） spectra and a series of TD-DFT calculations have been used to analyze trends in the electronic structures. Significant differences are observed in the optical spectra when solvents of differing polarity are used,which can be assigned to the effect of NH-tautomerism.

Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (<i>Phaseolus vulgaris</i>L.) under <i>in Vitro</i>Conditions by Scanning Electronic Microscopy

Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes development in four Costa Rican bean varieties (Brunca, Huetar, Guaymi and Bribri) cultivated on MS media with or without 5 mg·L–1 de N6-benzylaminopurine (BAP) by scanning electron microscopy (SEM) was developed in the present work. Micrographs showed similarities and differences in the ultrastructure of the apical dome, epidermal surface, stomata and different types of trichomes in the varieties cultivated on organogenesis media. Genotypes with advantageous morphological characteristics for genetic transformation, in particular an exposed apical dome, were identified. This work will contribute to the optimization of the in vitro regeneration of four common bean varieties.

High Efficiency and Light Mobile Electronic Business System Based on Mobile Agent Middleware

Mobile Network technology has been being the research focus during the 1990’s. The middleware technology is imported for the sake of running distributed transaction smoothly. In this paper, a mobile agent based middleware high efficiency mobile electronic business oriented middleware (HEMEBOM) is designed and implemented based on the requirement and background of collaborative electronic business. Its architecture, elements and excellent properties are mainly focused. Then high efficiency mobile electronic business system μMcommerce is built using HEMEBOM.

Mass-producible low-cost flexible electronic fabrics for azo dye wastewater treatment by electrocoagulation

Electrocoagulation is progressively becoming an ecologically friendly water treatment method owing to its lack of secondary pollution,high active ingredient concentration,high treatment effectiveness,simple equipment,and simplicity of automated control implementation.Herein,electrocoagulation is offered as a method for treating wastewater containing azo dyes using a revolutionary flexible electronic fabric that can be mass-producible at a reasonable price.A computer-controlled machine embroiders 316L stainless steel fiber(316L SSF)onto an insulating fabric to manufacture a flexible electronic device of cathode and anode with a monopolar arrangement on the fabric surface.Using methyl orange(MO)solution to simulate azo dye wastewater,the decolorization rate of 500 ml MO reached 99.25% under the conditions of 50 mg·L^(-1)initial mass concentration,120 min electrolysis time,15 mA·g^(-1)current density,1 cm electrode spacing,0.1 mol·L^(-1)NaCl,pH 7.6,200 r·min^(-1)rotational speed of the stirrer,and 22-25℃ room temperature.In addition,it is feasible to embroider flexible electronic fabrics with varied sizes and numbers of electrodes based on the amount of treated sewage to increase the degradation rate,which has significant practical application value.

Magnetic Properties and Electronic Structure of ThCo₄B

We present density functional theory DFT ab initio calculation of the electronic and magnetic properties of ThCo4B compound using the self-consistent full-potential linearized augmented plane wave (FPLAPW) method as implemented in the Wien2k package. The influence of the local environment on the Co magnetic moments is discussed by comparing the magnetic and electronic properties of ThCo4B to its parent ThCo5 compound. The total magnetic moment in these two compounds is dominated by the Co moment. The Spin orbit interaction affects the electronic structure and spin-density maps of the p-state of Th.

Application of Data Mining Technique in Business to Consumer Electronic Commerce

In B2C e-commerce activities, it is involving vast amounts of customer data information. Introducing data mining technology to the electronic commerce, it provides a large number of valuable business information to electronic business enterprise, enhances the core competition of the electronic commerce enterprise. This paper starts from the model of data mining, analyzes the steps of the data mining and puts forward the application of data mining technology in B2C e-commerce.

Electronic Absorption Spectra and Third-Order Nonlinear Optical Property of Dinaphtho[2,3-b:2’,3’-d]Thiophene-5,7,12,13- Tetraone (DNTTRA) and Its Phenyldiazenyl Derivatives: DFT Calculations

Third-order nonlinear optical (NLO) materials have broad application prospects in high-density data storage, optical computer, modern laser technology, and other high-tech industries. The structures and frequencies of Dinaphtho[2,3-b:2’,3’-d]thiophene-5,7,12,13-tetraone (DNTTRA) and its 36 derivatives containing azobenzene were calculated by using density functional theory B3LYP and M06-2X methods at 6-311++g(d, p) level, respectively. Besides, the atomic charges of natural bond orbitals (NBO) were analyzed. The frontier orbitals and electron absorption spectra of A-G5 molecule were calculated by TD-DFT (TD-B3LYP/6-311++g(d, p) and TD-M06-2X/6-311++g(d, p)). The NLO properties were calculated by effective finite field FF method and self-compiled program. The results show that 36 molecules of these six series are D-π-A-π-D structures. The third-order NLO coefficients γ (second-order hyperpolarizability) of the D series molecules are the largest among the six series, reaching 107 atomic units (10-33 esu) of order of magnitude, showing good third-order NLO properties. Last, the third-order NLO properties of the azobenzene ring can be improved by introducing strong electron donor groups (e.g. -N(CH3)2 or -NHCH3) in the azobenzene ring, so that the third-order NLO materials with good performance can be obtained.

The Role of Electronic Bill of Lading and Challenges to the Current Legal Framework

Many instruments have helped traders to effectively communicate,establish contracts,manage risk and protect their benefits while collaborating with people who have diverse cultural backgrounds.The B/L(bill of lading)has been one of the most significant documents in the shipping industry since it was invented in the 13th century in Italy.It is an evidence of existing contracts between the shipper and the carrier,in which both of them agreed to deliver the goods safely from the port of loading to the port of discharge.However,many problems are triggered by requiring the presentation of the original paper B/L at the discharging port so that the buyer gets the cargo in time.As of today,many attempts were carried out to develop an electronic equivalent to the paper B/L.Additionally,many practices were formed in order for these electronic equivalents to be legally reinforced and enhanced.This article presents the main transitional stages of these efforts and the status of this progress.It analyses the legal background which has been established to validate these ventures and the option of using an electronic B/L in order for the ships to be able to deliver the goods without the presentation of the original document.In the meantime,this article highlights potential areas for further investigation.

Theoretical Studies on the Mechanism of the Proton-transfer-coupled Electron Transfer Reactions Between Menaquinone Q_A and Ubiquinone Q_B in the Bacterial Photosynthetic Reaction Center of Rhodopseudomona viridis

The mechanism of the proton_transfer_coupled electron transfer (PT_ET) reactions between the menaquinone Q A (MQ 1) and ubiquinone Q B (UQ 1) in the bacterial photosynthetic reaction center of Rhodopseudomona viridis was studied by using the B3LYP/6_31G(d) method. The changes of standard Gibbs free energy ΔG 0 of all possible reactions followed the ET reaction (1) were calculated. The results indicated that: (1) according to the ΔG 0 values of corresponding reactions, UQ 1 could not accept two electrons from MQ - 1 continually without the coupled proton transfer reactions. Because of ΔG 0 2b 0, ΔG 0 3b 0 and ΔG 0 4b 0, the corresponding PT_ET reactions could take place along with reactions (2b), (3b) and (4b) sequentially; (2) on the gaseous condition, the first and second transferred protons (H +(1) and H +(2)) from the surrounding amino acid residues or water molecules will combine with the oxygen No.7 and oxygen No.8 of UQ 1, respectively. On the condition of protein surroundings (by SCRF model, ε =4.0), the results are converse but the energy difference between the combination of H +(1) and H +(2) with UQ - 1 is quite small. The difference of ΔG 0 values between the corresponding reactions in gaseous surroundings and the SCRF model is not significant; (3) the PT_ET reactions between MQ 1 - and UQ 1 - should be as follows: MQ 1 -+UQ 1→MQ 1+UQ 1 - (1) UQ 1 - ( O (7) )+H +( HisL 190)→UQ 1H(2b) ( Gas ) or UQ 1 - ( O (8) )+H +(H 2O)→UQ 1H (2b') ( SCRF ) or UQ 1 - ( O (8) )+H + ( ArgL 217)→UQ 1H(2b') ( SCRF ) MQ 1 -+UQ 1H→MQ 1+UQ 1H - (3b) ( Gas ) MQ 1 -+UQ 1H→MQ 1+UQ 1H -(3b') ( SCR F) UQ 1H -+H +(H 2O)→UQ 1H 2(4b) ( Gas ) or UQ 1H -+H + ( ArgL 217)→UQ 1H 2 (4b) ( Gas ) or UQ 1H -+H + ( HisL 190)→UQ 1H 2 (4b') ( SCRF )

基于R-L分数阶定义的CCM Flyback变换器建模与分析

针对电容电感都为分数阶这一事实,利用分数阶微积分R-L定义和状态空间平均法,对工作在电感电流连续模式(CCM)下的分数阶Flyback变换器进行建模与分析。推导CCM分数阶Flyback变换器的静态工作点和小信号传递函数以及变换器工作在CCM模式下的条件。其次,讨论Caputo模型与R-L导数模型的区别,得出在R-L分数阶定义下直流静态工作点不仅与占空比相关,而且还与电感和电容的阶数以及负载有关。最后,在PSIM中搭建R-L分数阶CCM Flyback变换器电路模型,得到不同分数阶电感电容阶数下分数阶Flyback变换器输出电压和电感电流的仿真波形,通过仿真结果与理论计算结果对比,验证所建模型的正确性,得出分数阶电感电容的阶数会影响闭环控制器的设计和分数阶模型能更加精确地描述Flyback变换器的工作特性。

迈腾B8L发动机故障诊断与修复

发动机电控系统诊断与修复是汽车技术国赛考核的重点,也是教学的难点。笔者结合自身参赛经历和发动机电控系统诊断与修复的教学经验,总结迈腾B8L1.4T发动机故障诊断与排除流程,对于教师教学备课和技能竞赛备赛具有参考意义。

非洲猪瘟病毒B602L蛋白的原核表达、纯化及多克隆抗体制备

【目的】非洲猪瘟病毒(African swine fever virus,ASFV)是对生猪养殖业危害极大的一种病原体,B602L蛋白作为其重要的非结构蛋白,是衣壳蛋白p72折叠为正确的三级结构的伴侣分子之一,在病毒组装成二十面体结构的过程中发挥着关键作用。特异性抗体对于抗非洲猪瘟免疫应答机制及血清学检测方法研究具有重要意义,本研究旨在获取ASFV B602L蛋白,并制备抗ASFV B602L蛋白的多克隆抗体,为ASFV B602L蛋白功能研究及相关诊断试剂的研发提供材料。【方法】以ASFV China/2018/AnhuiXCGQ株的B 602 L基因为研究对象,应用生物信息学软件对B602L蛋白的理化性质、信号肽、跨膜结构、抗原性和蛋白结构进行分析后,利用同源重组的方法将其克隆至原核表达载体pET-28a(+)中,转化大肠杆菌BL21(DE3)感受态细胞后经诱导表达获得B602L重组蛋白,利用镍柱亲和层析进行蛋白纯化,通过SDS-PAGE和Western blotting对B602L蛋白纯化效果进行验证。将纯化后的B602L蛋白免疫BALB/c小鼠制备多克隆抗体,通过间接ELISA方法测定多克隆抗体效价,通过Western blotting和Dot blotting检测多克隆抗体特异性。【结果】生物信息学分析表明,B602L蛋白属于稳定性亲水蛋白,无跨膜区和信号肽。二级结构主要含有α-螺旋(54.91%)、延伸链(9.81%)和无规则卷曲(32.08%)。成功构建了ASFV B602L重组质粒,SDS-PAGE和Western blotting结果显示,重组菌pET-28a-B602L-BL21在16℃、0.5 mmol/L IPTG诱导下高效表达,获得的重组蛋白主要表达在BL21菌株上清中,大小约为70 ku,且能与ASFV阳性血清特异性结合。间接ELISA检测结果显示,制备的抗B602L鼠源多克隆抗体效价达1∶409600,Western blotting和Dot blotting试验结果表明,抗B602L鼠源多克隆抗体能够特异性识别B602L蛋白。【结论】原核表达的B602L重组蛋白具有良好的免疫原性,能够与ASFV阳性血清特异性结合,制备的鼠抗ASFV B602L蛋白多克隆抗体具备较好的生物学活性。本试验结果可为进一步研究ASFV B602L蛋白的生物学功能和非洲猪瘟诊断方法的建立提供生物材料和理论支撑。

The research progress of an E//B neutral particle analyzer

An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of fast ions in the HL-2A/3 tokamak.The E//B NPA contains three main units,i.e.the stripping unit,the analyzing unit and the detection unit.A gas stripping chamber was adopted as the stripping unit.The results of the simulations and beam tests for the stripping chamber are presented.Parallel electric and magnetic fields provided by a NdFeB permanent magnet and two parallel electric plates were designed and constructed for the analyzing unit.The calibration of the magnetic and electric fields was performed using a 50 kV electron cyclotron resonance ion source(ECRIS)platform.The detection unit consists of 32lutetium-yttrium oxyorthosilicate(LYSO)detector modules arranged in two rows.The response functions ofα,hydrogen ions(H^(+),H_(2)^(+)and H_(3)^(+))andγfor a detector module were measured with^(241)Am,^(137)Cs and^(152)Eu sources together with the 50 kV ECRIS platform.The overall results indicate that the designed E//B NPA device is capable of measuring the intensity of neutral hydrogen and deuteron atoms with energy higher than 20 keV.

双偶氮苯-二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮衍生物分子的二阶非线性光学性质

使用密度泛函理论(DFT)M06-2X方法、采用6-311+g(d,p)基组,分别对26个双偶氮-二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮衍生物分子进行结构优化与频率计算;使用含时密度泛函理论(TD-DFT)TD-M06-2X方法计算了a1~d6分子的前线分子轨道与电子吸收光谱,采用有效场FF方法研究了二阶非线性光学性质(NLO).研究结果表明,26个噻蒽四酮类衍生物分子的能隙在1.33—2.02 eV范围,归属于有机半导体;最低能量吸收峰波长在601.8~609.5nm范围;在增大分子的二阶非线性光学系数β_(μ)(或β_(0))值方面,含相同偶氮苯基团或含不同偶氮苯基团分别引入到二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮分子两侧的2,10位优于2,9位,在2,10位分别端接含推、拉基团的偶氮苯优于含相同给电子基团的偶氮苯.在偶氮苯苯环对位分别端接强吸电子基(-NO_(2))与强供电子基(如-N(CH_(3))_(2)、-N(Ph)_(3)、-N-苯基咔唑等)可增强体系的二阶非线性光学性能,获得性能良好的非线性光学材料.

MyD88L265P及CD79B基因突变对原发性中枢神经系统淋巴瘤预后的影响

目的探究MyD88L265P及CD79B基因突变对原发性中枢神经系统淋巴瘤(PCNSL)患者预后的影响。方法收集2018年8月-2020年11月于兰州大学第二医院经开颅手术或立体定位活检术确诊的18例免疫功能正常(无HIV感染及免疫抑制药服用病史)的PCNSL患者临床资料进行回顾性分析。分别采用实时荧光定量PCR和一代基因测序技术检测18例PCNSL患者肿瘤组织中MyD88L265P和CD79B基因突变情况。针对可能与PCNSL首次无疾病进展生存期(PFS)和总生存期相关的指标行单因素分析,并采用Cox回归进行多因素分析。结果18例PCNSL肿瘤组织MyD88L265P突变率为38.9%,CD79B突变率为33.3%,MyD88L265P/CD79B共突变率为27.8%。单因素分析结果显示,病灶多发、病灶深部受累、肿瘤组织CD79B突变患者的PFS时间更短(P<0.05);多因素分析结果显示,病灶深部受累(HR=0.135,95%CI 0.023~0.799,P<0.05)、肿瘤组织CD79B突变(HR=0.149,95%CI 0.028~0.800,P<0.05)是PCNSL患者预后不良的独立危险因素。结论本组PCNSL患者肿瘤组织MyD88L265P、CD79B突变频率较高,这两种基因突变尤其是CD79B突变可能与PCNSL不良预后相关。

A transmission electron microscopy study of the diversity of Candida albicans cells induced by Euphorbia hirta L.leaf extract in vitro

Objective:To determine the major changes in the microstructure of Candida albicans(C. albicans) after treatment with Euphorbia hirta(E.hirta) L.leaf extract.Methods:Transmission electron microscopy was used to study the ultrastructural changes caused by E.hirta extract on C. albicans cells al various exposure time.Results:It was found that the main abnormalities were the alterations in morphology,lysis and complete collapse of the yeast cells after 36 h of exposure to the extract.Whereas the control cultures showed a typical morphology of Candida with a uniform central density,typically structured nucleus,and a cytoplasm with several elements of endomembrane system and enveloped by a regular,intact cell wall.Conclusions:The significant antifungal activity shown by this methanol extract of E.hirta L.suggests its potential against infections caused by C.albicans.The extract may be developed as an anticandidal agent.

A study on the energetic electron precipitation observed by CSES

High energy particles are the main target of satellite space exploration; particle storm events are closely related to solar activity,cosmic ray distribution, and magnetic storms. The commonly seen energetic particle(electron) precipitation anomalies include mainly the inner and outer Van Allen radiation belts, the South Atlantic Anomaly, and the anomalous stripes excited by artificial electromagnetic waves. The China Seismo-Electromagnetic Satellite(CSES), launched in February of 2018, provides a platform for studying ionospheric particle disturbances. This paper reports the first studies of electron precipitation phenomenon based on high energy particle data from the CSES. We find that the global distribution of electron fluxes in the low energy band(0.1–3 MeV) can relatively well reflect the anomalous precipitation belt, which is consistent with results based on the DEMETER satellite, indicating that the quality of the lowenergy band payload of the CSES is good. In addition, this paper makes an in-depth study of the electron precipitation belt excited by the NWC artificial VLF electromagnetic transmitter located in Australia, which appears as a typical wisp structure on the energy spectrum. The magnetic shell parameter L corresponding to the precipitation belt ranges from 1.44 to 1.74, which is close to the L value(～1.45) of the NWC transmitter; the energy of the precipitation electrons is between 100 keV and 361.57 keV, among which the precipitation of 213.73 keV electrons is most conspicuous.

汽油车无法发动综合故障快速诊断研究分析——以大众迈腾B8L为例

本文主要分析汽油发动机作为动力主体的燃油车辆无法发动的具体故障现象以及根据故障现象快速判断故障方向的方法。详细阐述以大众迈腾B8L为例的汽油车无法发动的故障现象与原因以及测量方法,总结了汽油车无法发动故障的快速诊断诀窍。内容包含汽油发动机电控系统的全部内容与要点,汽油车仍旧是社会交通工具的主体,本文仍具有一定的研究价值与意义。

Evaluation of the Potential of Araucaria angustifolia Seeds as Source of Oligosaccharides, Resistant Starch and Growth of Probiotic Bacteria

“Pinhão”, the seed of Araucaria angustifolia, is an important food, being part of the eating habits of Indigenous communities. In this study, we evaluated the oligosaccharide content, resistant starch and the growth of probiotic bacteria. GF4 (1-fructofuranosylnystose) was the main fructo-oligosaccharides found, in higher contents compared to other food sources. Maltooligosaccharides (MOS) represented the main part of the oligosaccharides profile of Brazilian pine seeds. In descending order of importance was maltoheptaose (G7), maltohexose (G6) and maltotriose (G3). The starches from the variety Sanct josephi presented the highest amount of resistant starch that could stimulate probiotic strains, mainly B. breve and L. plantarum, and may have a prebiotic effect, potentially promoting health benefits. This study advances the understanding of the chemical composition of the main portion of the “pinhão” enhancing awareness of its potential as a healthy food source, contributing to different uses and indirectly with the species preservation.