The education and management of college student Party members is the focus of student Party building work in colleges and universities.In the context of students second classroom being conducted in the academy,the man...The education and management of college student Party members is the focus of student Party building work in colleges and universities.In the context of students second classroom being conducted in the academy,the management of student Party members should enhance their Party spirit cultivation through improving their self-management and self-monitoring.After the comprehensive reform of Ningxia University,Runze College targeted the characteristics of agricultural majors and set up functional Party groups to enable student Party members to play a pioneering and exemplary role in the second classroom.Runze College relies on the academy system and breaks through the traditional one-way management mode led by Party branch teachers in the second classroom of college students.It leverages the role of functional Party groups in off campus science and technology academies,and integrates Party building work into daily teaching and research and solving agricultural production technology problems for local villagers,achieving the goal of promoting and integrating Party building and business work,and improving each other.展开更多
The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In h...The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration.展开更多
On Februar 22~(nd),2021,the State Councilissued Guiding Opinions on Accelerating the Establishment and Improvement of Green and Low-carbon Circular Economic System(hereinafter referred to as the Guiding Opinions)Accor...On Februar 22~(nd),2021,the State Councilissued Guiding Opinions on Accelerating the Establishment and Improvement of Green and Low-carbon Circular Economic System(hereinafter referred to as the Guiding Opinions)According to the Guiding Opinions, establishing and improving the country's green.展开更多
This article employs the theory of conceptual metaphor to conduct a thorough analysis and exploration of translation strategies for the metaphors in Song Ci. Ci, a treasure in ancient Chinese literature, makes frequen...This article employs the theory of conceptual metaphor to conduct a thorough analysis and exploration of translation strategies for the metaphors in Song Ci. Ci, a treasure in ancient Chinese literature, makes frequent use of metaphor. Its unique form of expression and abundant artistic effects often create difficulties in translation. This study selects some representative metaphor examples from Song Ci to explore the patterns and strategies in the translation process. The study reveals that in face of the metaphors in Song Ci, translators need to comprehensively apply strategies such as retaining the metaphor, replacing the metaphor, and translating the literal meaning to ensure the quality of translation while restoring the aesthetic charm of the original metaphor.展开更多
Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat (Triti-cum aestivumL.). At present, little is known about the effect of tilage and straw mulch on the ...Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat (Triti-cum aestivumL.). At present, little is known about the effect of tilage and straw mulch on the root system of wheat under dryland areas in southwestern China. The aim of this study was to evaluate the effect of three tilage treatments (no-til, NT; rotary til, RT; conventional til, CT) and two crop residue management practices (straw mulch, ML; non-straw mulch, NML) on stand establishment, root growth and grain yield of wheat. NT resulted in lower soil cover thickness for the wheat seed, higher number of uncovered seeds, lower percentage of seedling-less ridges and lower tiler density compared to RT and CT; ML resulted in higher tiler density compared to NML. Straw mulching resulted in more soil water content and root length density (RLD) at most of the growth stages and soil depths. The maximum RLD, root surface area density and root dry matter density were obtained under NT. In the topmost 10 cm soil layer, higher RLD values were found under NT than those under RT and CT. There were no signiifcant differences in the yield or yield components of wheat among the tilage treatments in 2011–2012, but NT resulted in a signiifcant higher yield compared to RT and CT in 2012–2013. Grain yield was signiifcantly higher in ML compared to in NML. A strong relationship was observed between the water-use efifciency and the grain yield. Both NT and ML proved beneifcial for wheat in term of maintaining higher tiler density, better soil water status and root growth, leading to a higher grain yield and enhanced water-use efifciency, especialy in a low rainfal year.展开更多
In the paper, the establishment, measurement, data-processing program and monitoring accuracy of the GPS seismic monitoring network in North China, especially in the Capital-Circle area, have been presented briefly. T...In the paper, the establishment, measurement, data-processing program and monitoring accuracy of the GPS seismic monitoring network in North China, especially in the Capital-Circle area, have been presented briefly. The relation of horizontal crustal deformation to tectonic movement, stress-field variation and seismicity has been analyzed in detail. The results indicate that the accuracy of GPS measurement has reached the order of 10-9 and the annual rate of horizontal crustal deformation in North China is about 4 ~5 mm. Horizontal crustal movement is a direct indication of the regional stress field. Therefore, by monitoring the time-sequence variation of horizontal crustal motion, it would be possible to investigate the change in the stress field, to analyze the tendency of seismicity and to determine the seismogenic zones.展开更多
Most intestinal parasites are cosmopolitan with the highest prevalence in the tropics and subtopics. Rural-to-urban migration rapidly increases the number of food eating places in towns and their environs. Some of the...Most intestinal parasites are cosmopolitan with the highest prevalence in the tropics and subtopics. Rural-to-urban migration rapidly increases the number of food eating places in towns and their environs. Some of these eating estabishments have poor sanitation and are overcrowded, facilitating disease transmission, especially through food-handling. Our investigations in Nairobi, therefore, were set to determine the presence of intestinal parasites in food-handlers with valid medical certificates. Direct and concentrated stool processing techniques were used. Chisquare test and ANOVA were used for data analysis. The parasites Ascaris lumbricoides, Entamoeba histolytica and Giardia lamblia were observed in certified food-handlers. Significant difference was found in parasite frequency by eating classes and gender (χ^2 = 9.49, P = 0.73), (F = 1.495, P = 0.297), but not in parasite occurrence between age brackets (χ^2 = 6.99, P = 0.039). The six-month medical certificate validity period may contribute significantly to the presence of intestinal parasites in certified food-handlers.展开更多
This article expounds the economic characteristics of circular agricultural technology.Since the circular agricultural technology has characteristics and externality of public goods,the government participation is nec...This article expounds the economic characteristics of circular agricultural technology.Since the circular agricultural technology has characteristics and externality of public goods,the government participation is necessary in building the technology system of circular agriculture,in order to avoid "free rider" behavior in the consumption of public goods and internalize externality.We analyze the orientation of government's role in building the technology system of circular agriculture:the government is the leader,designer,organizer and regulator in building the technology system of circular agriculture;the government orientates the four roles precisely in building the technology system of circular agriculture,to ensure that they do not cross the line,but be indispensable all the while.展开更多
The membrane trafficking of cation-independent mannose 6-phosphate receptor(CI-M6PR) between the transGolgi network(TGN) and endosomal compartments is not only critical for maintaining lysosomal function but also ...The membrane trafficking of cation-independent mannose 6-phosphate receptor(CI-M6PR) between the transGolgi network(TGN) and endosomal compartments is not only critical for maintaining lysosomal function but also a well-known event for understanding molecular and cellular mechanisms in retrograde endosome-to-TGN trafficking.Although it has been well established in literature that the C-terminus of bovine CI-M6PR determines its retrograde trafficking,it remains unclear whether the luminal domain of the protein plays a role on these sorting events.In this study,we found that partial deletion of luminal domain of human CI-M6PR mistargeted the mutant protein to nonTGN compartments.Moreover,replacing the luminal domain of both bovine and human CI-M6PR with that from irrelevant membrane proteins such as CD8 or Tac also altered the TGN targeting of the chimeric proteins.On the other hand,only short sequence from HA fused with the transmembrane domain and C-terminus of the receptor,HA-hCIM6PR-tail,resulted in its preferential targeting to TGN as for the full length receptor,strongly suggesting that sorting of the receptor may be influenced by luminal sequence.Furthermore,using this luminal truncated form of HA-hCIM6 PR as a model cargo,we found that the trafficking of the chimeric protein was regulated by the retromer complex through interacting with SNX5.In conclusion,our study strongly suggested that the disrupted luminal domain from hCI-M6PR or other irrelevant membrane proteins interfere with the process of membrane trafficking and TGN targeting of CI-M6PR.展开更多
BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are...BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.展开更多
[Objective]The aim of this study was to explore the feasibility of three kinds of urea formaldehyde foam substrate in tall fescue establishment as well as to study on evaluation of turf quality.[Method]The visual esti...[Objective]The aim of this study was to explore the feasibility of three kinds of urea formaldehyde foam substrate in tall fescue establishment as well as to study on evaluation of turf quality.[Method]The visual estimates,ecological quality estimates and utility quality estimates were used as the 1st level indicators,and the density,color,texture,uniformity,green period,cover degree,biomass,emergence energy,growth rate and turf-forming time were used as the corresponding 2nd level indicators.With T.L.Satty's method Analytic Hierarchy Process(AHP) and the fuzzy synthesis evaluation method,the weight ratio of matrix was obtained and the synthetic evaluation result was accorded with facts,then it could be regarded as the satisfying value of weight.[Result]The quality of three covering soil treatments was better than that of uncovering treatment and conventional treatment,the quality synthetic evaluation of F1 was the best,which was about 0.770.[Conclusion]Urea formaldehyde foam substrate showed high quality for turf establishment,which could be further spread and applied.展开更多
Treatment of terminal system is mainly through the interface communication protocol for treatment planningsystem to achieve the control of various kinds of medical equipments, including primary collimator, range shift...Treatment of terminal system is mainly through the interface communication protocol for treatment planningsystem to achieve the control of various kinds of medical equipments, including primary collimator, range shifter,scatterer, ridge type filter, multi-leaf collimator, etc. TPS was based on the radioactive source and the patientmodeling to simulate the plan implementation of radiotherapy. The basic structure of block diagram is shown inFig. 1.展开更多
The establishment meeting of International Society of Bionic Engineering (ISBE) convened grandly at Zhuhai,China in September 14-16, 2010. Related officials and representatives attended the opening ceremony, who camef...The establishment meeting of International Society of Bionic Engineering (ISBE) convened grandly at Zhuhai,China in September 14-16, 2010. Related officials and representatives attended the opening ceremony, who camefrom the Research Councils, UK, the National Natural Science Foundation of America, the Bionic Society of Ger-展开更多
The relationship between social justice and economic law is existed. Economic law is a general term of legal nomas to adjust social relations occurred in the course of state regulation of the economy, so that to prote...The relationship between social justice and economic law is existed. Economic law is a general term of legal nomas to adjust social relations occurred in the course of state regulation of the economy, so that to protect state regulation, and promote social and economic coordination, stability and development. From the view of the generation of the law, economic law is generated after the emergence of the socialization of production and monopoly to protect the public interest. Economic law is bound to reflect social justice in the redistribution process of interests. Legislative intent of economic law to maintain a fair society is reflected through the redistribution process of the interests. Redistribution of the economic law is an important means to achieve social justice.展开更多
A model for evaluating the capacity of the comprehensive prevention system in a region in terms of economic losses is established in this paper by using fuzzy mathematics.The authors have explored the weak links of th...A model for evaluating the capacity of the comprehensive prevention system in a region in terms of economic losses is established in this paper by using fuzzy mathematics.The authors have explored the weak links of the system mentioned above and suggested an effective procedure of increasing the capacity of earthquake resistance and disaster prevention in a region.Through taking suitable means of decision making,the mathematical model presented in this paper can be used for reference during establishing the comprehensive prevention system in a region to reduce the earthquake disasters.展开更多
The freshwater cichlid, Sarotherodon galilaeus (Linné, 1758) is an economically and commercially important fisheries species that has naturally colonized and dominated a sand-dragged man-made freshwater lake of A...The freshwater cichlid, Sarotherodon galilaeus (Linné, 1758) is an economically and commercially important fisheries species that has naturally colonized and dominated a sand-dragged man-made freshwater lake of Ahozon (South-Benin) where the species made about 85.21% of the fish community. We investigated the feeding ecology of S. galilaeus in order to evaluate resource exploitation and the establishment of this species in Lake Ahozon. During wet, flood and dry seasons, 1189 individuals of S. galilaeus have been monthly sampled in the open water and in the aquatic vegetation habitats. The study showed that S. galilaeus consumed about seventy (70) food items dominated by algae (52.88%) composed of 28 families and 52 genera from blue-green algae, green algae, desmids, and diatoms, sand particles (23.95%), detritus (12.27%) and protozoans (7.68%). Minor preys were rotifers, copepods, cladodera, crustacea and insect parts. Foods items with higher diet occurrence were sand particles with a percentage occurrence of 72.33%, detritus (69.47%), and some algae such as Scenedesmus (58.96%), Closterium (55.68%), and Microcystis (51.30%). Significant (P ≤ 0.05) ontogenetic variations of empty stomachs were recorded. S. galilaeus exhibited a wide diet breadth (DB) ranging between 5.55 and 7.29 that tended to increase with fish sizes. Diet overlaps (Øjk) varied from 0.77 to 0.97, indicating high diet similarities between different life stage categories. The slope b = 0.560 obtained from Log (body weight)-Log (gut length) linear regression, and the mean ratio of GL/SL = 6.72 ± 2.23 support our findings that S. galilaeus exhibited detritivorous, herbivorous and omnivorous food habits. The broad spectrum of food resources consumed/utilized and the high diet breadth recorded leading to allometric growth, the active breeding and the high propagation of the species, were some indicators of the successful establishment of S. galilaeus in the man-made lake of Ahozon. Sustainable fisheries/ aquaculture exploitation of S. galilaeus and successful valorization of the man-made lake of Ahozon require an integrated management of the lake including the prevention of domestic waste dumpings, the protection of the foraging and spawning grounds, the fertilization of the lake, the introduction of predators to control the population of S. galilaeus , the protection of lake margin to avoid erosion, a planned harvest of the fish stock and a periodic ecological monitoring of Lake Ahozon.展开更多
1. Aims and Significance "The aims and significance of the trial establishment of Sino-foreign coinvested foreign trade companies are to use the sales channels and nets of foreign businessmen to expand China’s e...1. Aims and Significance "The aims and significance of the trial establishment of Sino-foreign coinvested foreign trade companies are to use the sales channels and nets of foreign businessmen to expand China’s exports and increase export results. Through trial establishment,展开更多
On Sept.27, 2005, China Certification and Accreditation Association was set up, which was a sign of standardization and legalization for China certification and accreditation. Furthermore, its main task includes suppo...On Sept.27, 2005, China Certification and Accreditation Association was set up, which was a sign of standardization and legalization for China certification and accreditation. Furthermore, its main task includes supporting fairness, gaining credit, promoting development and building a harmonious society.展开更多
基金School-level Theoretical Research Project on Grassroots Party Building at Ningxia University in 2023(NXDXDJ202335).
文摘The education and management of college student Party members is the focus of student Party building work in colleges and universities.In the context of students second classroom being conducted in the academy,the management of student Party members should enhance their Party spirit cultivation through improving their self-management and self-monitoring.After the comprehensive reform of Ningxia University,Runze College targeted the characteristics of agricultural majors and set up functional Party groups to enable student Party members to play a pioneering and exemplary role in the second classroom.Runze College relies on the academy system and breaks through the traditional one-way management mode led by Party branch teachers in the second classroom of college students.It leverages the role of functional Party groups in off campus science and technology academies,and integrates Party building work into daily teaching and research and solving agricultural production technology problems for local villagers,achieving the goal of promoting and integrating Party building and business work,and improving each other.
文摘The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration.
文摘On Februar 22~(nd),2021,the State Councilissued Guiding Opinions on Accelerating the Establishment and Improvement of Green and Low-carbon Circular Economic System(hereinafter referred to as the Guiding Opinions)According to the Guiding Opinions, establishing and improving the country's green.
文摘This article employs the theory of conceptual metaphor to conduct a thorough analysis and exploration of translation strategies for the metaphors in Song Ci. Ci, a treasure in ancient Chinese literature, makes frequent use of metaphor. Its unique form of expression and abundant artistic effects often create difficulties in translation. This study selects some representative metaphor examples from Song Ci to explore the patterns and strategies in the translation process. The study reveals that in face of the metaphors in Song Ci, translators need to comprehensively apply strategies such as retaining the metaphor, replacing the metaphor, and translating the literal meaning to ensure the quality of translation while restoring the aesthetic charm of the original metaphor.
基金supported by the China Agriculture Research System(CARS-3)the Public Welfare Industry(Agriculture)Scientific Research of China(200903010-06)
文摘Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat (Triti-cum aestivumL.). At present, little is known about the effect of tilage and straw mulch on the root system of wheat under dryland areas in southwestern China. The aim of this study was to evaluate the effect of three tilage treatments (no-til, NT; rotary til, RT; conventional til, CT) and two crop residue management practices (straw mulch, ML; non-straw mulch, NML) on stand establishment, root growth and grain yield of wheat. NT resulted in lower soil cover thickness for the wheat seed, higher number of uncovered seeds, lower percentage of seedling-less ridges and lower tiler density compared to RT and CT; ML resulted in higher tiler density compared to NML. Straw mulching resulted in more soil water content and root length density (RLD) at most of the growth stages and soil depths. The maximum RLD, root surface area density and root dry matter density were obtained under NT. In the topmost 10 cm soil layer, higher RLD values were found under NT than those under RT and CT. There were no signiifcant differences in the yield or yield components of wheat among the tilage treatments in 2011–2012, but NT resulted in a signiifcant higher yield compared to RT and CT in 2012–2013. Grain yield was signiifcantly higher in ML compared to in NML. A strong relationship was observed between the water-use efifciency and the grain yield. Both NT and ML proved beneifcial for wheat in term of maintaining higher tiler density, better soil water status and root growth, leading to a higher grain yield and enhanced water-use efifciency, especialy in a low rainfal year.
文摘In the paper, the establishment, measurement, data-processing program and monitoring accuracy of the GPS seismic monitoring network in North China, especially in the Capital-Circle area, have been presented briefly. The relation of horizontal crustal deformation to tectonic movement, stress-field variation and seismicity has been analyzed in detail. The results indicate that the accuracy of GPS measurement has reached the order of 10-9 and the annual rate of horizontal crustal deformation in North China is about 4 ~5 mm. Horizontal crustal movement is a direct indication of the regional stress field. Therefore, by monitoring the time-sequence variation of horizontal crustal motion, it would be possible to investigate the change in the stress field, to analyze the tendency of seismicity and to determine the seismogenic zones.
文摘Most intestinal parasites are cosmopolitan with the highest prevalence in the tropics and subtopics. Rural-to-urban migration rapidly increases the number of food eating places in towns and their environs. Some of these eating estabishments have poor sanitation and are overcrowded, facilitating disease transmission, especially through food-handling. Our investigations in Nairobi, therefore, were set to determine the presence of intestinal parasites in food-handlers with valid medical certificates. Direct and concentrated stool processing techniques were used. Chisquare test and ANOVA were used for data analysis. The parasites Ascaris lumbricoides, Entamoeba histolytica and Giardia lamblia were observed in certified food-handlers. Significant difference was found in parasite frequency by eating classes and gender (χ^2 = 9.49, P = 0.73), (F = 1.495, P = 0.297), but not in parasite occurrence between age brackets (χ^2 = 6.99, P = 0.039). The six-month medical certificate validity period may contribute significantly to the presence of intestinal parasites in certified food-handlers.
文摘This article expounds the economic characteristics of circular agricultural technology.Since the circular agricultural technology has characteristics and externality of public goods,the government participation is necessary in building the technology system of circular agriculture,in order to avoid "free rider" behavior in the consumption of public goods and internalize externality.We analyze the orientation of government's role in building the technology system of circular agriculture:the government is the leader,designer,organizer and regulator in building the technology system of circular agriculture;the government orientates the four roles precisely in building the technology system of circular agriculture,to ensure that they do not cross the line,but be indispensable all the while.
基金supported by the National Nature Science Foundation of China to Y.Liu(Grant No.31371436 and No.8157051134)Y.Huang(Grant No.81500678)the laboratory start-up grant from Nanjing Medical University to Y.Liu
文摘The membrane trafficking of cation-independent mannose 6-phosphate receptor(CI-M6PR) between the transGolgi network(TGN) and endosomal compartments is not only critical for maintaining lysosomal function but also a well-known event for understanding molecular and cellular mechanisms in retrograde endosome-to-TGN trafficking.Although it has been well established in literature that the C-terminus of bovine CI-M6PR determines its retrograde trafficking,it remains unclear whether the luminal domain of the protein plays a role on these sorting events.In this study,we found that partial deletion of luminal domain of human CI-M6PR mistargeted the mutant protein to nonTGN compartments.Moreover,replacing the luminal domain of both bovine and human CI-M6PR with that from irrelevant membrane proteins such as CD8 or Tac also altered the TGN targeting of the chimeric proteins.On the other hand,only short sequence from HA fused with the transmembrane domain and C-terminus of the receptor,HA-hCIM6PR-tail,resulted in its preferential targeting to TGN as for the full length receptor,strongly suggesting that sorting of the receptor may be influenced by luminal sequence.Furthermore,using this luminal truncated form of HA-hCIM6 PR as a model cargo,we found that the trafficking of the chimeric protein was regulated by the retromer complex through interacting with SNX5.In conclusion,our study strongly suggested that the disrupted luminal domain from hCI-M6PR or other irrelevant membrane proteins interfere with the process of membrane trafficking and TGN targeting of CI-M6PR.
基金the National Natural Science Foundation of China,No.82060551and Lanzhou Chengguan District Science and Technology Planning Project,No.2019JSCX0092.
文摘BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.
基金Supported by Beijing Excellent Talent Training Project(20071D020500055)Beijing Municipal Academy of Agricultural Youth Foundation of China(2007020414)+1 种基金Beijing Municipal Science and Technology Commission of Township Building Based onan Innovative New Agricultural Technology Research and Demonstration(Z080005032508024)Slow-release Fertilizer TechnologyResearch Project(2008BAD4B04)~~
文摘[Objective]The aim of this study was to explore the feasibility of three kinds of urea formaldehyde foam substrate in tall fescue establishment as well as to study on evaluation of turf quality.[Method]The visual estimates,ecological quality estimates and utility quality estimates were used as the 1st level indicators,and the density,color,texture,uniformity,green period,cover degree,biomass,emergence energy,growth rate and turf-forming time were used as the corresponding 2nd level indicators.With T.L.Satty's method Analytic Hierarchy Process(AHP) and the fuzzy synthesis evaluation method,the weight ratio of matrix was obtained and the synthetic evaluation result was accorded with facts,then it could be regarded as the satisfying value of weight.[Result]The quality of three covering soil treatments was better than that of uncovering treatment and conventional treatment,the quality synthetic evaluation of F1 was the best,which was about 0.770.[Conclusion]Urea formaldehyde foam substrate showed high quality for turf establishment,which could be further spread and applied.
文摘Treatment of terminal system is mainly through the interface communication protocol for treatment planningsystem to achieve the control of various kinds of medical equipments, including primary collimator, range shifter,scatterer, ridge type filter, multi-leaf collimator, etc. TPS was based on the radioactive source and the patientmodeling to simulate the plan implementation of radiotherapy. The basic structure of block diagram is shown inFig. 1.
文摘The establishment meeting of International Society of Bionic Engineering (ISBE) convened grandly at Zhuhai,China in September 14-16, 2010. Related officials and representatives attended the opening ceremony, who camefrom the Research Councils, UK, the National Natural Science Foundation of America, the Bionic Society of Ger-
文摘The relationship between social justice and economic law is existed. Economic law is a general term of legal nomas to adjust social relations occurred in the course of state regulation of the economy, so that to protect state regulation, and promote social and economic coordination, stability and development. From the view of the generation of the law, economic law is generated after the emergence of the socialization of production and monopoly to protect the public interest. Economic law is bound to reflect social justice in the redistribution process of interests. Legislative intent of economic law to maintain a fair society is reflected through the redistribution process of the interests. Redistribution of the economic law is an important means to achieve social justice.
文摘A model for evaluating the capacity of the comprehensive prevention system in a region in terms of economic losses is established in this paper by using fuzzy mathematics.The authors have explored the weak links of the system mentioned above and suggested an effective procedure of increasing the capacity of earthquake resistance and disaster prevention in a region.Through taking suitable means of decision making,the mathematical model presented in this paper can be used for reference during establishing the comprehensive prevention system in a region to reduce the earthquake disasters.
文摘The freshwater cichlid, Sarotherodon galilaeus (Linné, 1758) is an economically and commercially important fisheries species that has naturally colonized and dominated a sand-dragged man-made freshwater lake of Ahozon (South-Benin) where the species made about 85.21% of the fish community. We investigated the feeding ecology of S. galilaeus in order to evaluate resource exploitation and the establishment of this species in Lake Ahozon. During wet, flood and dry seasons, 1189 individuals of S. galilaeus have been monthly sampled in the open water and in the aquatic vegetation habitats. The study showed that S. galilaeus consumed about seventy (70) food items dominated by algae (52.88%) composed of 28 families and 52 genera from blue-green algae, green algae, desmids, and diatoms, sand particles (23.95%), detritus (12.27%) and protozoans (7.68%). Minor preys were rotifers, copepods, cladodera, crustacea and insect parts. Foods items with higher diet occurrence were sand particles with a percentage occurrence of 72.33%, detritus (69.47%), and some algae such as Scenedesmus (58.96%), Closterium (55.68%), and Microcystis (51.30%). Significant (P ≤ 0.05) ontogenetic variations of empty stomachs were recorded. S. galilaeus exhibited a wide diet breadth (DB) ranging between 5.55 and 7.29 that tended to increase with fish sizes. Diet overlaps (Øjk) varied from 0.77 to 0.97, indicating high diet similarities between different life stage categories. The slope b = 0.560 obtained from Log (body weight)-Log (gut length) linear regression, and the mean ratio of GL/SL = 6.72 ± 2.23 support our findings that S. galilaeus exhibited detritivorous, herbivorous and omnivorous food habits. The broad spectrum of food resources consumed/utilized and the high diet breadth recorded leading to allometric growth, the active breeding and the high propagation of the species, were some indicators of the successful establishment of S. galilaeus in the man-made lake of Ahozon. Sustainable fisheries/ aquaculture exploitation of S. galilaeus and successful valorization of the man-made lake of Ahozon require an integrated management of the lake including the prevention of domestic waste dumpings, the protection of the foraging and spawning grounds, the fertilization of the lake, the introduction of predators to control the population of S. galilaeus , the protection of lake margin to avoid erosion, a planned harvest of the fish stock and a periodic ecological monitoring of Lake Ahozon.
文摘1. Aims and Significance "The aims and significance of the trial establishment of Sino-foreign coinvested foreign trade companies are to use the sales channels and nets of foreign businessmen to expand China’s exports and increase export results. Through trial establishment,
文摘On Sept.27, 2005, China Certification and Accreditation Association was set up, which was a sign of standardization and legalization for China certification and accreditation. Furthermore, its main task includes supporting fairness, gaining credit, promoting development and building a harmonious society.