Introduction: The transition from primary to secondary school is a period when physical activity (PA) declines. Interventions delivered during curriculum time have had limited impact on PA. The after-school period may...Introduction: The transition from primary to secondary school is a period when physical activity (PA) declines. Interventions delivered during curriculum time have had limited impact on PA. The after-school period may offer a valuable opportunity to increase children’s PA. In order to identify how best to implement after-school PA interventions for older primary school children, more information regarding the provision of after-school clubs is required. This paper examined the current after-school club provision of English primary schools. Methods: All state-funded primary schools in England (n = 15,307) were sent an online questionnaire in two phases during 2013. Schools were asked about the active and non-active after-school clubs on offer to year 5 and year 6 pupils and the days on which they run, the number of children attending each after-school club, who funds the club and who leads the club. Results: Responding schools (501) were reasonably representative of the national profile. Of the 2413 clubs reported, more non-active than active clubs (5.3 vs. 4.8 per school) were described. Football was the most frequently reported activity (offered by 79.5% of schools), with netball and dance being offered by 45.3% and 44.1% of schools, respectively. A high proportion of clubs was funded by schools or parents (88.6%) and more than 40% were led by external parties. Conclusions: A number of PA programmes are provided after-school but current provision is dominated by team sports and thus, there is a need for non-sport specific PA clubs. Furthermore, there is a need to find cost-effective methods of delivering after-school PA programmes.展开更多
We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in...We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in this paper. Another T lymphocytes,such as peripheral T lymphocytes donated by a maIe patient with multiple sclerosis, could be transformed into a malignant lymphoma line by using filtered supernatant of the CM-1 cultured medium, thus the CM-2 cell line u'as estabIished. The CM-1 and CM-2 cells were transplanted by subcutaneous inoculation into nude mice, and could cause the occurrenceof typical maIignant lymphoma. The observation of eIectron micrographs suggested the existence of virions in the CM-1 and CM-2 cells, and these virions were similar toretrovirus in the ultra-structure characteristics. lt was found that this virus possesses reverse transcriptase activity. ResuIts obtained from serological assay, molecular hybridization and PCR excluded the existence of other human viruses, which were commonly usedin our laboratory. The unknown virus possesses strong transformation activity, and probably is a new retro virus. Meanwhile, the work on the clone and sequence analysis ofthis virus are being carried out.展开更多
A language is a carrier of a certain culture. Consequently,for the purpose of studying English well, it is essential for us to understand the contents of the English-spoken countries. This essay involves some ways for...A language is a carrier of a certain culture. Consequently,for the purpose of studying English well, it is essential for us to understand the contents of the English-spoken countries. This essay involves some ways for high school students to learn the culture of the English-spoken countries.展开更多
Language education centers primarily on fostering linguistic skills and language communication skills for students. In the process of teaching Chinese language, it is in the classroom that is the place tofoster studen...Language education centers primarily on fostering linguistic skills and language communication skills for students. In the process of teaching Chinese language, it is in the classroom that is the place tofoster student’s linguistic competence and language communication capacity. Therefore, classroom teaching becomes the main place tofoster the language ability and language communication capacity for students while the extracurricular activities are the place for students to apply the knowledge they have learned in the classroom in to the real world. Conducting a survey to investigate extracurricular activities for Chinese language majors at Thai Nguyen University, School of Foreign Languages, the paper proposes solutions to improve the effectiveness of extracurricular activities for students and create excitement for students in learning.展开更多
自20世纪80年代起,校外活动在美国家庭生活中逐渐占据重要地位,并具备了显著的竞争性意义,这也导致中产及以上阶层父母将大量金钱和精力投入到儿童校外生活中。希拉里·弗瑞德曼的Playing to Win:Raising Children in a Competitive...自20世纪80年代起,校外活动在美国家庭生活中逐渐占据重要地位,并具备了显著的竞争性意义,这也导致中产及以上阶层父母将大量金钱和精力投入到儿童校外生活中。希拉里·弗瑞德曼的Playing to Win:Raising Children in a Competitive Culture一书深入剖析了美国中产及以上阶层家庭校外活动的历史背景、活动内容以及竞争资本的转化机制等,详细记录了家长们如何通过国际象棋、足球、舞蹈等校外竞技活动,看似在“玩”,实际上却是引导儿童内化竞争意识、积累竞争资本,揭示了美国父母“鸡娃”现象与社会竞争加剧、阶层固化之间的内在联系。这对于理解当前中国中产阶层家庭的教育选择具有重要参考价值。展开更多
The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineur...The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.展开更多
文摘Introduction: The transition from primary to secondary school is a period when physical activity (PA) declines. Interventions delivered during curriculum time have had limited impact on PA. The after-school period may offer a valuable opportunity to increase children’s PA. In order to identify how best to implement after-school PA interventions for older primary school children, more information regarding the provision of after-school clubs is required. This paper examined the current after-school club provision of English primary schools. Methods: All state-funded primary schools in England (n = 15,307) were sent an online questionnaire in two phases during 2013. Schools were asked about the active and non-active after-school clubs on offer to year 5 and year 6 pupils and the days on which they run, the number of children attending each after-school club, who funds the club and who leads the club. Results: Responding schools (501) were reasonably representative of the national profile. Of the 2413 clubs reported, more non-active than active clubs (5.3 vs. 4.8 per school) were described. Football was the most frequently reported activity (offered by 79.5% of schools), with netball and dance being offered by 45.3% and 44.1% of schools, respectively. A high proportion of clubs was funded by schools or parents (88.6%) and more than 40% were led by external parties. Conclusions: A number of PA programmes are provided after-school but current provision is dominated by team sports and thus, there is a need for non-sport specific PA clubs. Furthermore, there is a need to find cost-effective methods of delivering after-school PA programmes.
文摘We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in this paper. Another T lymphocytes,such as peripheral T lymphocytes donated by a maIe patient with multiple sclerosis, could be transformed into a malignant lymphoma line by using filtered supernatant of the CM-1 cultured medium, thus the CM-2 cell line u'as estabIished. The CM-1 and CM-2 cells were transplanted by subcutaneous inoculation into nude mice, and could cause the occurrenceof typical maIignant lymphoma. The observation of eIectron micrographs suggested the existence of virions in the CM-1 and CM-2 cells, and these virions were similar toretrovirus in the ultra-structure characteristics. lt was found that this virus possesses reverse transcriptase activity. ResuIts obtained from serological assay, molecular hybridization and PCR excluded the existence of other human viruses, which were commonly usedin our laboratory. The unknown virus possesses strong transformation activity, and probably is a new retro virus. Meanwhile, the work on the clone and sequence analysis ofthis virus are being carried out.
文摘A language is a carrier of a certain culture. Consequently,for the purpose of studying English well, it is essential for us to understand the contents of the English-spoken countries. This essay involves some ways for high school students to learn the culture of the English-spoken countries.
文摘Language education centers primarily on fostering linguistic skills and language communication skills for students. In the process of teaching Chinese language, it is in the classroom that is the place tofoster student’s linguistic competence and language communication capacity. Therefore, classroom teaching becomes the main place tofoster the language ability and language communication capacity for students while the extracurricular activities are the place for students to apply the knowledge they have learned in the classroom in to the real world. Conducting a survey to investigate extracurricular activities for Chinese language majors at Thai Nguyen University, School of Foreign Languages, the paper proposes solutions to improve the effectiveness of extracurricular activities for students and create excitement for students in learning.
文摘自20世纪80年代起,校外活动在美国家庭生活中逐渐占据重要地位,并具备了显著的竞争性意义,这也导致中产及以上阶层父母将大量金钱和精力投入到儿童校外生活中。希拉里·弗瑞德曼的Playing to Win:Raising Children in a Competitive Culture一书深入剖析了美国中产及以上阶层家庭校外活动的历史背景、活动内容以及竞争资本的转化机制等,详细记录了家长们如何通过国际象棋、足球、舞蹈等校外竞技活动,看似在“玩”,实际上却是引导儿童内化竞争意识、积累竞争资本,揭示了美国父母“鸡娃”现象与社会竞争加剧、阶层固化之间的内在联系。这对于理解当前中国中产阶层家庭的教育选择具有重要参考价值。
基金supported by the National Important Novel Medicine Research Project (2012ZX09304010, 2013ZX09102062)the National Natural Science Foundation of China (31270849)
文摘The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.
