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Unenumerated Rights Clauses and the System of Restriction on Fundamental Rights——Another Construction Plan for Article 51 of the Constitution of PRC
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作者 杜强强 QIAN Chuijun 《The Journal of Human Rights》 2023年第1期91-108,共18页
According to constitutional jurisprudence in China,Article 51 of the Constitution is an unenumerated restrictive clause.Such theoretical construction fails to justify the differentiated restrictions established in the... According to constitutional jurisprudence in China,Article 51 of the Constitution is an unenumerated restrictive clause.Such theoretical construction fails to justify the differentiated restrictions established in the Constitution and makes it difficult to explain the constitutional status of human dignity. Article 51 implies the possibility of other theoretical constructions. It is not an unenumerated restriction clause but an unenumerated rights clause. It aims to provide guarantees for general freedom of action not enumerated in the Constitution rather than restricting fundamental rights. The actual benefit of this kind of theoretical construction lies in that it can establish the basis of the constitutional text for general freedom of action and promote the people’s correct understanding of the model of restriction on fundamental rights established by the Constitution of PRC. 展开更多
关键词 restriction on fundamental rights ¿legal construction ¿general freedom of action
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Based on network pharmacology and molecular docking technology to explore the mechanism of Panax notoginseng in the treatment of membranous nephropathy
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作者 LI Ying MAO Yan-ping +1 位作者 WANG Yi-ping ZHANG Lei 《Journal of Hainan Medical University》 CAS 2023年第19期36-43,共8页
Objective:Based on network pharmacology and molecular docking technology to explore the mechanism of Professor Cao Enze's application of Panax notoginseng in the treatment of membranous nephropathy.Methods:TCMSP d... Objective:Based on network pharmacology and molecular docking technology to explore the mechanism of Professor Cao Enze's application of Panax notoginseng in the treatment of membranous nephropathy.Methods:TCMSP database was used to obtain the effective components and corresponding target information of Panax notoginseng,and Gene Cards database was used to obtain the disease target genes of membranous nephropathy.The intersection targets of the two were taken and the Venn diagram was drawn.The STRING database was used to obtain the protein interaction relationship,and the PPI network diagram was constructed by Cytoscape 3.9.1 software to screen out the core targets of Panax notoginseng in the treatment of membranous nephropathy.GO function and KEGG pathway enrichment analysis were performed using the David database to obtain the potential pathway of Panax notoginseng in the treatment of membranous nephropathy.Finally,Autodock software was used to verify the molecular docking of the main active components of the drug with the core targets.Results:A total of 7 effective components such as quercetin,ginsenoside rh2,Mandenol and Stigmasterol were retrieved,and 127 potential targets of Panax notoginseng in the treatment of membranous nephropathy were screened out.By PPI network topology analysis,23 core targets such as JUN,TP53,RELA,AKT1 and MAPK1 were screened out.GO functional enrichment analysis contained 703 biological processes,55 cell components and 121 molecular functions,and KEGG signal pathway enrichment analysis enriched 171 signal pathways.The results of molecular docking showed that there was a strong binding ability between the main core targets and the main components of Panax notoginseng.Conclusion:Through network pharmacology,it is concluded that Panax notoginseng treats membranous nephropathy through multiple targets and multiple pathways,which provides a theoretical basis for subsequent basic research. 展开更多
关键词 Panax notoginsen Network pharmacology Molecular docking Membranous nephropathy Effective com-ponents Mechanism of action experience of famous doctors
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The Formative Role of Art: John Dewey's Art as Quotidian Experience
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作者 Mei-Hsin Chert 《Journal of Literature and Art Studies》 2017年第12期1574-1579,共6页
关键词 艺术教育 杜威 教育艺术 亚里士多德 审美艺术 审美意识 敏感性 性行为
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FUNDAMENTAL GROUPS OF CLOSED POSITIVELY CURVED MANIFOLDS WITH ALMOST DISCRETE ABELIAN GROUP ACTIONS
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作者 王雨生 《Acta Mathematica Scientia》 SCIE CSCD 2010年第1期203-207,共5页
Let M be a closed n-manifold of positive sectional curvature. Assume that M admits an effective isometrical T1× Zpk-action with p prime. The main result of the article n+1 for n 〉 5, then there exists a positiv... Let M be a closed n-manifold of positive sectional curvature. Assume that M admits an effective isometrical T1× Zpk-action with p prime. The main result of the article n+1 for n 〉 5, then there exists a positive constant p(n), is that ifk=lforn=3or k〉 n+1/4 for n≥5,then there exists a positive constant p(n),depending only on n, such that π1 (M) is cyclic if p ≥ p(n). 展开更多
关键词 fundamental groups positive sectional curvature group actions
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Neutrinos from CERN, Reaching Too Early to Gran Sasso, Do Not Exceed the Velocity of Light. They in Fact Reveal the True Physical Mechanism of Gravity
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作者 Jacob Schaf 《Journal of Modern Physics》 2018年第12期2125-2134,共10页
In 2011 neutrinos from CERN in Geneva-CH were announced to reach to the OPERA Lab in Gran Sasso-IT 60 ns earlier than light. In reality, the velocity of the neutrinos was compared, not with the measured one-way veloci... In 2011 neutrinos from CERN in Geneva-CH were announced to reach to the OPERA Lab in Gran Sasso-IT 60 ns earlier than light. In reality, the velocity of the neutrinos was compared, not with the measured one-way velocity of light, however with the presumed velocity of light c. As this conclusion breaks the light postulate, the data were withdrawn. In fact, to compare the neutrino velocity with the presumed velocity of light violates a fundamental precept of scientific methodologies. Such a comparison could make a sense only if the velocity of both neutrinos and light had been measured along the same path in vacuum. Actually the absence of the solar gravitational slowing of the GPS clocks, absence of light anisotropy with respect to earth etc. demonstrates that the Higgs Quantum Fluid Space (HQFS), giving mass to the elementary particles and thus ruling their inertial motion, is moving round the sun according to a Keplerian velocity field, consistently with the planetary motions. It is also moving round earth consistently with the orbital motion of the Moon. The Keplerian velocity fields are the quintessence of the gravitational fields. In the earth’s field, the velocity of the HQFS achieves 7.91 km/sec on surface and drags both the neutrinos and light toward the East. In the South-East direction, from CERN to OPERA Lab, making &sim;58 degrees with the Meridians, this drag adds 6.7 km/sec to the conventional light velocity c, making neutrinos from CERN (and light) to reach the OPERA Lab ~60 ns earlier than presumed by the current theories. 展开更多
关键词 fundamental PHYSICS theORY of Relativity RELATIVISTIC Effects RELATIVISTIC Experiments Gravitational PHYSICS Higgs theORY Neutrino PHYSICS
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Einstein’s Theory of Relativity in the Scenario of the Higgs Quantum Space Dynamics
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作者 Jacob Schaf 《Journal of Modern Physics》 2018年第5期1111-1143,共33页
This work is concerned about the true physical mechanism of gravity. The Higgs theory introduces important changes in Einstein’s view about the nature of empty space, about the meaning of motions and about the nature... This work is concerned about the true physical mechanism of gravity. The Higgs theory introduces important changes in Einstein’s view about the nature of empty space, about the meaning of motions and about the nature of the gravitational physics. The Higgs Quantum Space (HQS) is a real quantum fluid spatial medium, giving inertial mass to the elementary particles by the Higgs mechanism. Therefore, the HQS rules the inertial motion of matter-energy and is the local ultimate reference for rest and for motions. In this new scenario, velocity with respect to the local HQS and not relative velocity is the origin of all the effects of motion. The HQS also necessarily is responsible for the gravitational fields because it is mass that creates the gravitational fields. The observed absence of the gravitational slowing of the GPS clocks by the solar field and the absence of light anisotropy with respect to earth are both the obvious signature of the true physical mechanism of gravity in action. These observations show that the HQS is moving itself round the sun according to a Keplerian velocity field, consistent with the planetary motions, which will be shown to accurately create, besides the observed gravitational dynamics, all the observed effects of the gravitational fields on light and on clocks. Current theories explain the absence of the solar gravitational slowing of the GPS clocks with base in the principle of equivalence. In the language of the present work, the HQS materializes the local Lorentz frames (LFs), turning them into local proper LFs, intrinsically stationary with respect to the local moving HQS. In its motions, the HQS carries these local proper LFs with it round the sun, so that the planets of the solar system are stationary with respect to them, which directly predicts the absence of the gravitational slowing of the GPS clocks by the solar field, the absence of light anisotropy with respect to earth and all the other effects of the gravitational fields observed on earth. 展开更多
关键词 fundamental Physics theORY of RELATIVITY RELATIVISTIC Effects TIME DILATION Gravitational TIME DILATION HIGGS theORY RELATIVISTIC Experiments
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The Role of Flow in Crisis Intervention and Emotional Counseling in Tree-Hole Rescue
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作者 WU Jiatong 《Psychology Research》 2022年第1期11-18,共8页
Flow is the experience of working full time and enjoying the flow of your life,regardless of the rewards,like a flowing stream of water.Based on the literature review,this paper discusses the role of flow in the actua... Flow is the experience of working full time and enjoying the flow of your life,regardless of the rewards,like a flowing stream of water.Based on the literature review,this paper discusses the role of flow in the actual rescue work,combined with the actual work of the rescue mission in the tree hole baby crisis intervention and emotional counseling and accompanying.The effect of flow intervention is positive.It can reduce the rumination of young tree hole babies and increase and enhance their sense of life meaning and subjective well-being.Since the term“Flow”is rarely used in the process of intervention,this paper also looks forward to the further application of flow intervention. 展开更多
关键词 flow experience crisis intervention ruminant thinking sense of meaning of life tree hole action
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Heparin-induced extracorporeal LDL-precipitation(HELP):Mode of action, safety and clinical experience(3)
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作者 P.Schuff-Werner 《中国血液净化》 2002年第4期1-3,共3页
  Safety and practicability of the HELP procedure   Compared to standard plasma exchange, HELP-apheresis is much better tolerated, primarily be-cause heparin rather than citrate is used for anti-coagulation and t...   Safety and practicability of the HELP procedure   Compared to standard plasma exchange, HELP-apheresis is much better tolerated, primarily be-cause heparin rather than citrate is used for anti-coagulation and therefore problems due to hypercalce-mia do not play a role.…… 展开更多
关键词 LDL Mode of action safety and clinical experience HELP Heparin-induced extracorporeal LDL-precipitation
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What Is the Missing Dark Energy in a Nutshell and the Hawking-Hartle Quantum Wave Collapse
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作者 Mohamed S. El Naschie 《International Journal of Astronomy and Astrophysics》 2013年第3期205-211,共7页
We reason that in quantum cosmology there are two kinds of energy. The first is the ordinary energy of the quantum particle which we can measure. The second is the dark energy of the quantum wave by quantum duality. B... We reason that in quantum cosmology there are two kinds of energy. The first is the ordinary energy of the quantum particle which we can measure. The second is the dark energy of the quantum wave by quantum duality. Because measurement collapses the Hawking-Hartle quantum wave of the cosmos, dark energy cannot be detected or measured in any conventional manner. The quantitative results are confirmed using some exact solutions for the hydrogen atom. In particular the ordinary energy of the quantum particle is given by E(0) = (/2)(mc2) where is Hardy’s probability of quantum entanglement, =( - 1)/2 is the Hausdorff dimension of the zero measure thin Cantor set modeling the quantum particle, while the dark energy of the quantum wave is given by E(D) = (5/2)(mc2) where is the Hausdorff dimension of the positive measure thick empty Cantor set modeling the quantum wave and the factor five (5) is the Kaluza-Klein spacetime dimension to which the measure zero thin Cantor set D(0) = (0,) and the thick empty set D(-1) = (1,) must be lifted to give the five dimensional analogue sets namely and 5 needed for calculating the energy density E(0) and E(D) which together add to Einstein’s maximal total energy density E(total) = E(0) + E(D) = mc2 = E(Einstein). These results seem to be in complete agreement with the WMAP, supernova and recent Planck cosmic measurement as well as the 2005 quantum gravity experiments of V. V. Nesvizhersky and his associates. It also confirms the equivalence of wormhole solutions of Einstein’s equations and quantum entanglement by scaling the Planck scale. 展开更多
关键词 KALUZA-KLEIN DARK ENERGY QUANTUM Particle as Zero SET QUANTUM WAVE as an Empty SET QUANTUM Gravity Experiments Hawking-Hartle WAVE DARK ENERGY of the QUANTUM WAVE Scaling the Planck Scale Equivalence of Einstein-Rosen Bridges Spooky Action at Distance
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共同富裕视域下的乡村教育振兴(笔谈)
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作者 李森 张鸿翼 +7 位作者 杜尚荣 汪莹 赵鑫 李敏 王天平 牌代琼 张姝 朱艳 《现代教育管理》 北大核心 2024年第2期22-42,共21页
党的二十大报告指出,中国式现代化是全体人民共同富裕的现代化。作为推进共同富裕的重要领域和组成部分,乡村教育振兴具体表现为乡村教育现代化发展和乡村教育全面振兴、乡村教育内涵式发展和乡村教育质量振兴、乡村文化特色彰显和乡村... 党的二十大报告指出,中国式现代化是全体人民共同富裕的现代化。作为推进共同富裕的重要领域和组成部分,乡村教育振兴具体表现为乡村教育现代化发展和乡村教育全面振兴、乡村教育内涵式发展和乡村教育质量振兴、乡村文化特色彰显和乡村教育资源共享振兴。共同富裕视域下的乡村教育具有文化性、育人性、整体性和生态性特征。共同富裕中全面富裕、共建共富和逐步富裕理念为乡村教育振兴指明了方向。乡村教育的内生动力、资源配置和文化供给为实现振兴提供支持,通过共生、共治、共建、共享等路径,构建乡村教育振兴助力共同富裕的新格局。未来的乡村教育需突显学校、社会、个体各层面的乡土化、现代化和共享化,从巩固、衔接、振兴三个实践向度,以政府顶层设计、内生格局构建、良性联动机制等构筑行动框架,统筹乡村教育发展规划、激发乡村教育永续动力、助推乡村教育高质量发展。 展开更多
关键词 共同富裕 乡村教育振兴 内涵特征 理论逻辑 历史经验 行动框架
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生涯教育的国家行动:国际经验及我国战略应对
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作者 索桂芳 《西北师大学报(社会科学版)》 北大核心 2024年第3期72-81,共10页
在当前落实立德树人根本任务、全面发展素质教育的大背景下,加强生涯教育具有重要的战略意义:是培养对国家、对社会、对民族真正有用人才的需要,是促进人终身发展、全面发展、个性发展的需要,是保障育人方式转变和新高考改革顺利推进的... 在当前落实立德树人根本任务、全面发展素质教育的大背景下,加强生涯教育具有重要的战略意义:是培养对国家、对社会、对民族真正有用人才的需要,是促进人终身发展、全面发展、个性发展的需要,是保障育人方式转变和新高考改革顺利推进的需要,也是应对百年未有之大变局和全球产业竞争的需要。成功推行生涯教育,需要国家层面强有力的保障和支持,国外将发展生涯教育上升为国家行动,强化立法保障与政策支持,强化组织保障与经费支持,强化顶层设计与战略指导,强化平台建设与公共服务提供,强化机制建立与环境氛围营造,强化师资队伍建设与专业发展,从而推动生涯教育在本国蓬勃有效地开展。借鉴国际经验,从我国实际出发,为使我国生涯教育走出当前困境,应从战略上提升对生涯教育的认识,增强社会认同;坚持立法先行,建立健全相关法律;加强组织机构建设,为推进生涯教育提供组织保障;建立长效机制,破解学校生涯教育面对的缺乏经费、师资以及职业体验机会等难题;强化战略指导,提供基本公共服务,完善对学校的考核评价。 展开更多
关键词 生涯教育 国家行动 国际经验 战略应对
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中国共产党领导中国式现代化的历史进程、独特优势与基本经验
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作者 臧秀玲 李政一 《南通大学学报(社会科学版)》 北大核心 2024年第3期1-9,159,共10页
中国式现代化形成于中国共产党领导人民艰苦奋斗与艰辛探索的伟大征程中。这一过程中,中国共产党以政党属性、政党使命、政党能力、政党组织、政党理论等独特优势为要素支撑成功推进现代化,形成了中国式现代化拓展的理论架构与实践样态... 中国式现代化形成于中国共产党领导人民艰苦奋斗与艰辛探索的伟大征程中。这一过程中,中国共产党以政党属性、政党使命、政党能力、政党组织、政党理论等独特优势为要素支撑成功推进现代化,形成了中国式现代化拓展的理论架构与实践样态。作为一种政党主导的现代化模式,中国式现代化创造了大党支撑和服务大国现代化建设的新典范,彰显出鲜明的政党逻辑,展现出显著的比较优势。立足新时代新征程,要在全面从严治党、坚持守正创新、站稳人民立场、坚持独立自主中持续推进中国式现代化。 展开更多
关键词 中国式现代化 党的领导 历史进程 独特优势 基本经验
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劳动课程体系建设的逻辑理路与实践策略
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作者 王明娣 冀晓雨 《当代教育与文化》 2024年第4期90-96,共7页
核心素养时代致力于促进学生核心素养发展,培育学生劳动素养是提升学生核心素养的必然要求。提高学生劳动素养,劳动教育需具备较为成熟、完备的课程体系。基于以劳育人、跨界整合、学科实践之维对劳动课程体系建设基本参照进行解析,从... 核心素养时代致力于促进学生核心素养发展,培育学生劳动素养是提升学生核心素养的必然要求。提高学生劳动素养,劳动教育需具备较为成熟、完备的课程体系。基于以劳育人、跨界整合、学科实践之维对劳动课程体系建设基本参照进行解析,从核心理念、学科属性、行动主体之维厘清劳动课程体系建设的逻辑理路,在此基础上提出立足以劳育人,实现五育协调并举;回归具身体验,促进学生劳动素养全面提升;跨越学科边界,系统建构劳动课程体系;回应文化时空特征,扩展劳动课程活动空间;提升教师专业行动力,推进课程专业化实施等劳动课程体系建设的实践策略。 展开更多
关键词 劳动课程 学科实践 具身体验 跨界 专业行动力
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基于“问-思-辨-行”的智慧化振动理论课程建设
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作者 王乐 王慧 杨智春 《高教学刊》 2024年第23期23-26,共4页
以提升学生对振动理论的理解及创新实践能力的培养为目标,结合“互联网+教育”技术,建设有趣易用的智慧化教学资源——含网页程序及微信小程序的互动虚拟实验平台,解决传统课堂缺乏对振动现象的全面展示、学生难以开展振动现象的自主探... 以提升学生对振动理论的理解及创新实践能力的培养为目标,结合“互联网+教育”技术,建设有趣易用的智慧化教学资源——含网页程序及微信小程序的互动虚拟实验平台,解决传统课堂缺乏对振动现象的全面展示、学生难以开展振动现象的自主探索等问题。进一步结合工程实践及课程思政的有机融入,提出学生深度参与的课堂教学模式——深度融合课程思政及工程实践的“问-思-辨-行”教学模式,以充分发挥理论与实践的相互促进作用,促进对学生处理振动问题及实践创新能力的培养。针对飞行器振动理论及应用课程的教学实践结果表明,课堂师生互动、生生互动氛围活跃,学生各项考核成绩均稳步提升,尤其是考核学生实践创新能力的综合性作业成绩显著提升。 展开更多
关键词 互联网+教育 智慧化教学 互动虚拟实验 “问-思-辨-行”教学模式 振动理论
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“枫桥经验”与诉源治理法治化
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作者 胡铭 王梓帆 《南都学坛(南阳师范学院人文社会科学学报)》 2024年第3期9-16,共8页
在习近平法治思想的指引下,“枫桥经验”与时俱进,创造性地解决了新时代的系列治理问题,逐渐发展为党领导基层治理的重要经验。当前我国基层矛盾纠纷仍呈高发态势,法院面临的“案多人少”问题依然严重,坚持和发展新时代“枫桥经验”,推... 在习近平法治思想的指引下,“枫桥经验”与时俱进,创造性地解决了新时代的系列治理问题,逐渐发展为党领导基层治理的重要经验。当前我国基层矛盾纠纷仍呈高发态势,法院面临的“案多人少”问题依然严重,坚持和发展新时代“枫桥经验”,推进矛盾纠纷前端预防化解和多元综合治理相结合的诉源治理,是防止我国成为“诉讼大国”的有效途径。应当在党委领导下构建诉源治理大格局,形成多元化解矛盾纠纷体系,推动矛盾调处机制法治化、智能化发展。 展开更多
关键词 诉源治理 枫桥经验 多元解纷 法治
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黄芪抑制胶质瘤的作用机制和基因靶点研究
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作者 陈潘迪 王洪财 +3 位作者 李振强 王东峰 叶耿帆 陈茂送 《浙江医学》 CAS 2024年第1期9-14,I0004,I0005,共8页
目的探讨黄芪抑制胶质瘤的作用机制和基因靶点。方法基于网络药理学分析获得黄芪可能治疗胶质瘤的基因靶点,采用基因本体论(GO)富集分析和京都基因和基因组数据库(KEGG)通路分析,构建蛋白质-蛋白质相互作用(PPI)网络并筛选出Hub基因,最... 目的探讨黄芪抑制胶质瘤的作用机制和基因靶点。方法基于网络药理学分析获得黄芪可能治疗胶质瘤的基因靶点,采用基因本体论(GO)富集分析和京都基因和基因组数据库(KEGG)通路分析,构建蛋白质-蛋白质相互作用(PPI)网络并筛选出Hub基因,最后构建疾病-药物-成分-靶点-通路的可视化网络。同时取胶质瘤细胞株U251进行细胞实验论证,采用细胞计数试剂盒、活死细胞双染色法检测不同浓度黄芪培养液对U251细胞存活率和凋亡的影响。结果共筛选出145个黄芪可能治疗胶质瘤的基因靶点,GO富集分析显示最主要的生物学功能包括不同蛋白结合、相同蛋白结合、RNA聚合酶Ⅱ启动子转录的正向调控、酶结合等,KEGG通路分析显示最主要的信号通路包括癌症通路、脂质与动脉粥样硬化、流体剪切应力和动脉粥样硬化、化学致癌-受体激活等。Hub基因关联程度从高到低依次为血管内皮生长因子A、蛋白激酶B(AKT)1、JUN、基质金属蛋白酶9、PTGS2、IL-6、IL-1B、CASP3、TP53、HIF1A。在所有生物活性成分中,MOL000098(槲皮素)的度值最高,为118;在所有信号通路中,hsa05200(癌症通路)的度值最高,为61;在所有基因靶点中,AKT1、RELA、PTGS2、MAPL1、TP53的度值较高,分别为21、20、20、19、18。细胞实验证实,不同浓度(1、5、10 mg/mL)黄芪培养液对U251细胞增殖均有抑制和促进凋亡作用,其作用强度呈浓度依赖性(均P<0.05)。结论黄芪对U251细胞增殖具有抑制和促进凋亡作用,主要基因靶点包括AKT1、RELA、PTGS2、MAPL1、TP53。 展开更多
关键词 黄芪 胶质瘤 网络药理学 基因靶点 作用机制 细胞实验
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整合网络药理学和体外实验探究夏枯草-半枝莲药对治疗乳腺癌的作用及机制
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作者 刘苏 陈洪晓 +3 位作者 金乐 张慧慧 张蕾 陈昭琳 《安徽医科大学学报》 CAS 北大核心 2024年第7期1134-1142,共9页
目的采用网络药理学和体外细胞实验探究夏枯草-半枝莲药对抗乳腺癌的作用机制。方法运用中药系统药理学数据库与分析平台筛选出夏枯草-半枝莲药对的有效成分和靶点;利用GeneCards、OMIM数据库找到乳腺癌靶点,进而构建药物-活性成分-关... 目的采用网络药理学和体外细胞实验探究夏枯草-半枝莲药对抗乳腺癌的作用机制。方法运用中药系统药理学数据库与分析平台筛选出夏枯草-半枝莲药对的有效成分和靶点;利用GeneCards、OMIM数据库找到乳腺癌靶点,进而构建药物-活性成分-关键靶点网络和蛋白-蛋白相互作用(PPI)网络;运用R语言进行GO功能和KEGG通路富集分析以及生存分析;将筛选出的活性成分和核心靶点进行分子对接验证;采用CCK-8法检测细胞活力;EdU、流式实验检测细胞增殖和凋亡情况;Western blot法检测p-AKT1、AKT1、β-catenin和c-MYC的蛋白表达水平。结果通过数据分析共筛选出有效成分36个,交集靶点105个,其核心成分是槲皮素、木犀草素、山奈酚、汉黄芩素、黄芩素;通过PPI和生存分析得出其关键靶点是AKT1、ESR1、CASP3、MYC;GO分析共包含4303条富集结果,KEGG分析共包含232条通路;分子对接显示核心成分与关键靶点均具有较强的结合能力。细胞实验证明,核心活性成分槲皮素可以抑制乳腺癌细胞的增殖并促进其凋亡(P<0.05),下调p-AKT1、β-catenin和c-MYC蛋白的表达水平(P<0.05)。结论夏枯草-半枝莲药对中活性成分槲皮素可能通过AKT1/β-catenin信号通路来发挥作用,为其治疗乳腺癌的作用机制研究提供了科学参考。 展开更多
关键词 网络药理学 夏枯草 半枝莲 分子对接 细胞实验 作用机制
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基于网络药理学及体外细胞实验分析生姜泻心汤治疗溃疡性结肠炎的潜在作用
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作者 熊诗琳 林小荷 +4 位作者 黄松 侯少贞 刘昌辉 姜小艳 梁健 《新中医》 CAS 2024年第5期36-43,共8页
目的:基于网络药理学及体外细胞实验分析生姜泻心汤治疗溃疡性结肠炎(UC)的潜在作用。方法:运用中医系统药理学数据库及分析平台(TCMSP)筛选生姜泻心汤的活性成分及相关靶点,利用GeneGards、在线人类孟德尔遗传(OMIM)、药物遗传学和药... 目的:基于网络药理学及体外细胞实验分析生姜泻心汤治疗溃疡性结肠炎(UC)的潜在作用。方法:运用中医系统药理学数据库及分析平台(TCMSP)筛选生姜泻心汤的活性成分及相关靶点,利用GeneGards、在线人类孟德尔遗传(OMIM)、药物遗传学和药物基因组学知识库(PharmGkb)、治疗靶点数据库(TTD)和开放数据药物和药物靶标数据库(DrugBank)筛选UC疾病靶点,将药物靶点与疾病靶点匹配所得交集靶点导入互作基因/蛋白质检索工具(STRING)数据库构建蛋白相互作用(PPI)网络,并构建药物-成分-靶点-疾病网络。采用分子对接分析核心靶点和活性成分结合力,并通过脂多糖(LPS)诱导人结直肠腺癌细胞(Caco2细胞)损伤模型,给予生姜泻心汤干预24 h后,采用qPCR和免疫荧光探索生姜泻心汤对核心靶点的作用。结果:生姜泻心汤治疗UC的有效成分共215个,靶点171个,其中潜在核心有效成分为姜烯酮A、槲皮素、山柰酚、汉黄芩素和柚皮素;核心靶点包括HIF1A、STAT3、CTNNB1、CASP3、AKT1、IL-1β、TP53、EGFR和JUN。分子对接结果显示,AKT1、TP53和IL-1β与姜烯酮A、槲皮素、山柰酚、汉黄芩素和柚皮素均有较强的结合能力。细胞实验发现,生姜泻心汤干预LPS刺激Caco2细胞模型,能显著地抑制AKT1、TP53和IL-1β基因和蛋白的表达。结论:生姜泻心汤能够调控AKT1、TP53和IL-1β等靶点,发挥治疗UC的作用。 展开更多
关键词 溃疡性结肠炎 生姜泻心汤 网络药理学 体外细胞实验 作用机制
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基于体外实验和网络药理学探讨沙生蜡菊醇提物治疗非酒精性脂肪肝炎的作用机制
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作者 崔琪悦 孙佳茜 +2 位作者 朱磊 马红梅 尤丽 《特产研究》 2024年第2期47-58,67,共13页
本研究采用体外试验和网络药理学方法,探究沙生蜡菊醇提物治疗非酒精性脂肪肝炎(Nonalcoholic steatohepatitis,NASH)的药效学基础和作用机制。用游离脂肪酸诱导HepG2细胞建立体外NASH模型,用不同浓度沙生蜡菊醇提物进行干预,发现其对于... 本研究采用体外试验和网络药理学方法,探究沙生蜡菊醇提物治疗非酒精性脂肪肝炎(Nonalcoholic steatohepatitis,NASH)的药效学基础和作用机制。用游离脂肪酸诱导HepG2细胞建立体外NASH模型,用不同浓度沙生蜡菊醇提物进行干预,发现其对于NASH细胞模型的脂质沉积有较好的改善作用;在体外进行了沙生蜡菊醇提物的油脂吸附试验和对脂肪酶活性影响试验,发现沙生蜡菊醇提物具有较好油脂吸附能力和抑制脂肪酶活性的能力,说明沙生蜡菊在体外有良好的降脂活性。在上述基础上,利用CNKI、万方数据库、Pubmed等数据库查找沙生蜡菊相关靶点,GeneCards、OMIM、TTD、DrugBank平台查找非酒精性脂肪肝炎相关蛋白,得到潜在活性成分33个,与NASH相关靶点88个;通过蛋白-蛋白互作分析得到核心靶点,选取排名靠前的10个,通过GO和KEGG富集分析及靶点-通路网络图表明,沙生蜡菊中主要活性成分通过多途径和多靶点的方式发挥治疗NASH的作用机制,其可能与糖尿病并发症中的AGE-RAGE信号通路、脂质和动脉粥样硬化通路、胰岛素抵抗通路、化学致癌-多活性氧通路、脂肪细胞因子信号通路、HIF-1信号通路等相关。利用AutoDockTools 1.5.7软件对核心靶点和核心成分进行分子对接,结果表明均具有较好的结合能。总之,本研究初步探讨了沙生蜡菊醇提物治疗NASH的“活性成分-靶点-通路”作用机制,为进一步验证其作用机制提供了参考,也为沙生蜡菊治疗NASH提供了理论依据。 展开更多
关键词 非酒精性脂肪肝炎 沙生蜡菊 体外实验 网络药理学 分子对接 作用机制
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“知行合一”中医内证体察课程教学模式的实践探讨
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作者 杜菁 肖微 +1 位作者 刘争强 章文春 《江西中医药大学学报》 2024年第3期111-114,共4页
中医内证体察学是中医学的重要组成部分,在中医学整个产生和发展过程中影响深远,是中医理论的基石,也是中医临床的基础和升华。以中医内证体察的主要教学实践内容——对形态结构的体察、人体之气的体察、经络之气的体察以及对神意的体... 中医内证体察学是中医学的重要组成部分,在中医学整个产生和发展过程中影响深远,是中医理论的基石,也是中医临床的基础和升华。以中医内证体察的主要教学实践内容——对形态结构的体察、人体之气的体察、经络之气的体察以及对神意的体察为切入点,提出“知行合一”的中医内证体察课程教学模式,说明将中医内证体察融入中医教学,对提高中医教学质量有积极推动作用,不仅有利于中医学生理解晦涩难懂的中医理论,切身体察到形态结构、气的真实性以及经络的循行等,满足中医学生学习中医的实践需求,体悟中医基础理论的精髓,提高中医专业学生的临床实践能力,树立中医学生对中医学理论体系的文化自信,而且对中医药学的传承和发展也有积极促进作用。 展开更多
关键词 知行合一 中医内证体察学 中医学教学模式 教学实践
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