Objective:To investigate the role and mechanism of adrenomedullin(AM) in diabetic nephropathy. Methods: This research observed the changes of the expression and secretion of AM,TGF-β1 in the cultured human mesangial ...Objective:To investigate the role and mechanism of adrenomedullin(AM) in diabetic nephropathy. Methods: This research observed the changes of the expression and secretion of AM,TGF-β1 in the cultured human mesangial cells under high glucose conditions and the contents of the laminin and type Ⅳ collagen in the supernatants,and the effect of intervention with AM on the changes. Results: High glucose condition resulted in increase in the expression and secretion of AM, TGF-β1,laminin and type Ⅳ collagen, and AM could reverse the influence of high glucose on the cultured human mesangial cells. Conclusion: The results showed that high glucose condition in one of the stimulating factors of AM,and the renal protective action of AM may be associated with suppression of TGF-β1 and reducing excessive accumulation of laminin and type Ⅳ collagen.展开更多
基金Nature Science Foundation of the Department of Eucation of Jiangsu Province(00KJD320017)
文摘Objective:To investigate the role and mechanism of adrenomedullin(AM) in diabetic nephropathy. Methods: This research observed the changes of the expression and secretion of AM,TGF-β1 in the cultured human mesangial cells under high glucose conditions and the contents of the laminin and type Ⅳ collagen in the supernatants,and the effect of intervention with AM on the changes. Results: High glucose condition resulted in increase in the expression and secretion of AM, TGF-β1,laminin and type Ⅳ collagen, and AM could reverse the influence of high glucose on the cultured human mesangial cells. Conclusion: The results showed that high glucose condition in one of the stimulating factors of AM,and the renal protective action of AM may be associated with suppression of TGF-β1 and reducing excessive accumulation of laminin and type Ⅳ collagen.
